SE8103735L - PROCEDURE FOR ACTIVATING CHOLESTEROLOXIDAS - Google Patents

PROCEDURE FOR ACTIVATING CHOLESTEROLOXIDAS

Info

Publication number
SE8103735L
SE8103735L SE8103735A SE8103735A SE8103735L SE 8103735 L SE8103735 L SE 8103735L SE 8103735 A SE8103735 A SE 8103735A SE 8103735 A SE8103735 A SE 8103735A SE 8103735 L SE8103735 L SE 8103735L
Authority
SE
Sweden
Prior art keywords
cholesterol
lipophilic
hydrophilic properties
detecting
active compound
Prior art date
Application number
SE8103735A
Other languages
Swedish (sv)
Inventor
P Roeschlau
G Lang
K Beaucamp
E Bernt
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of SE8103735L publication Critical patent/SE8103735L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Abstract

Cholesterol oxidase is activated by adding a surface-active compound with lipophilic and hydrophilic properties. This method is advantageously used for detecting and for determining cholesterol and cholesterol esters in body fluids by oxidising the cholesterol with oxygen in the presence of cholesterol oxidase to cholestenone and hydrogen peroxide and detecting the hydrogen peroxide by peroxidase-catalysed oxidation of an oxidation catalyst, wherein the cholesterol oxidase is activated before it is used by adding a surface-active compound with lipophilic and hydrophilic properties. The diagnostic composition for carrying out this method is distinguished by consisting of an absorbent carrier or a plastic film which is impregnated with cholesterol oxidase, a system for detecting H2O2, buffer and at least one surface-active compound with lipophilic and hydrophilic properties, or contains these embedded in it.

Claims (1)

PatentkravPatent claims 1. , FOrfarande for aktivering av !ran detergentspAr befriad kcaesteroloxidas, kAnnetecknat darav, att minst en ytaktiv fOrening med lipofila och hydrofila egenskaper, vilken innehAller minst en 011-grupp, tillsattes Xolestercloidasen i en verksam mAngd fore dess anvAndning fOr bestamning av kolester01.1. A process for activating calcium detergent-liberated detergent trace, characterized in that at least one surfactant compound having lipophilic and hydrophilic properties, which contains at least one 011 group, was added to the cholesterol ester chloride in an effective amount prior to its use to determine carbon.
SE8103735A 1973-12-07 1981-06-15 PROCEDURE FOR ACTIVATING CHOLESTEROLOXIDAS SE8103735L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2361169A DE2361169C3 (en) 1973-12-07 1973-12-07 Process for the activation of cholesterol oxidase freed from detergent traces
DE742439348A DE2439348C3 (en) 1973-12-07 1974-08-16 Diagnostic agent for the detection and determination of cholesterol and cholesterol esters in body fluids

Publications (1)

Publication Number Publication Date
SE8103735L true SE8103735L (en) 1981-06-15

Family

ID=25766212

Family Applications (2)

Application Number Title Priority Date Filing Date
SE7415324A SE7415324L (en) 1973-12-07 1974-12-06
SE8103735A SE8103735L (en) 1973-12-07 1981-06-15 PROCEDURE FOR ACTIVATING CHOLESTEROLOXIDAS

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SE7415324A SE7415324L (en) 1973-12-07 1974-12-06

Country Status (18)

Country Link
JP (1) JPS5733954B2 (en)
AR (1) AR201067A1 (en)
AT (1) AT338441B (en)
BE (1) BE822843A (en)
CA (1) CA1044582A (en)
CH (1) CH613046A5 (en)
DD (1) DD115759A5 (en)
DE (2) DE2361169C3 (en)
DK (1) DK136293C (en)
FI (1) FI57616C (en)
FR (1) FR2254026B1 (en)
GB (1) GB1435400A (en)
HK (1) HK49279A (en)
HU (1) HU170109B (en)
IL (1) IL46073A (en)
IT (1) IT1026827B (en)
NL (2) NL170022C (en)
SE (2) SE7415324L (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL48804A (en) * 1975-01-29 1979-05-31 Baxter Travenol Lab Imminological reagent comprising a mixture of polyethyleneglycol and a nonionic surfactant
DE2612725C3 (en) * 1976-03-25 1979-05-03 Boehringer Mannheim Gmbh, 6800 Mannheim Method and reagent for the determination of cholesterol
US4161425A (en) * 1976-07-01 1979-07-17 Beckman Instruments, Inc. Enzymatic reagent system for total cholesterol assay using oxygen-rate method
US4275152A (en) 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
CA1104041A (en) * 1977-02-03 1981-06-30 Charles T. Goodhue Hydrolysis of protein-bound cholesterol esters
US4275151A (en) 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
US4298345A (en) 1977-11-21 1981-11-03 Damon Corporation Method and apparatus for chemical spot test analysis
US4409326A (en) * 1980-07-10 1983-10-11 Modrovich Ivan Endre Stabilized enzymatic solutions and method for determining total cholesterol in human serum
JPS5791197A (en) * 1980-11-20 1982-06-07 Toyobo Co Ltd Reagent for measuring activity of substrate or enzyme
DE3249743C2 (en) * 1982-02-18 1990-10-04 Amano Pharmaceutical Co., Ltd., Nagoya, Aichi, Jp
FR2556010B1 (en) * 1982-02-18 1988-07-29 Amano Pharma Co Ltd METHOD FOR THE QUANTITATIVE DETERMINATION OF PHYSIOLOGICAL COMPONENTS IN BIOLOGICAL FLUIDS
US5047327A (en) * 1982-04-02 1991-09-10 Ivan E. Modrovich Time-stable liquid cholesterol assay compositions
DE3374019D1 (en) * 1982-07-23 1987-11-12 Wako Pure Chem Ind Ltd Process for quantitative determination of substrate treated with oxidase

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT988069B (en) * 1971-09-22 1975-04-10 Nat Res Dev ENZYMATIC PREPARATION AND METHOD FOR ITS PRODUCTION

Also Published As

Publication number Publication date
IL46073A (en) 1977-05-31
NL170022C (en) 1982-09-16
NL7415037A (en) 1975-06-10
DE2439348A1 (en) 1976-03-04
FI57616B (en) 1980-05-30
DK637074A (en) 1975-07-28
DE2439348B2 (en) 1977-09-15
FI57616C (en) 1980-09-10
GB1435400A (en) 1976-05-12
AU7557874A (en) 1976-05-20
DK136293C (en) 1978-02-20
DE2361169B2 (en) 1978-01-19
IT1026827B (en) 1978-10-20
DE2361169C3 (en) 1978-09-14
NL8201349A (en) 1982-08-02
DE2361169A1 (en) 1975-06-19
JPS5733954B2 (en) 1982-07-20
FR2254026A1 (en) 1975-07-04
HU170109B (en) 1977-04-28
CA1044582A (en) 1978-12-19
DE2439348C3 (en) 1979-03-01
AT338441B (en) 1977-08-25
AR201067A1 (en) 1975-02-06
SE7415324L (en) 1975-06-09
IL46073A0 (en) 1975-02-10
FR2254026B1 (en) 1977-11-04
JPS5089588A (en) 1975-07-18
HK49279A (en) 1979-07-27
DK136293B (en) 1977-09-26
DD115759A5 (en) 1975-10-12
NL170022B (en) 1982-04-16
CH613046A5 (en) 1979-08-31
BE822843A (en) 1975-06-02
FI351374A (en) 1975-06-08
ATA974074A (en) 1976-12-15

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