GB1070603A - Composition for the determination of isocitrate dehydrogenase activity - Google Patents

Composition for the determination of isocitrate dehydrogenase activity

Info

Publication number
GB1070603A
GB1070603A GB3754665A GB3754665A GB1070603A GB 1070603 A GB1070603 A GB 1070603A GB 3754665 A GB3754665 A GB 3754665A GB 3754665 A GB3754665 A GB 3754665A GB 1070603 A GB1070603 A GB 1070603A
Authority
GB
United Kingdom
Prior art keywords
isocitrate
composition
dichloroindophenol
determination
methosulphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3754665A
Inventor
Claus Wagenknecht
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AWD Pharma GmbH and Co KG
Original Assignee
Arzneimittelwerk Dresden GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arzneimittelwerk Dresden GmbH filed Critical Arzneimittelwerk Dresden GmbH
Priority to GB3754665A priority Critical patent/GB1070603A/en
Publication of GB1070603A publication Critical patent/GB1070603A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Abstract

A composition for the determination of isocitrate dehydrogenase, e.g. in body fluids of animals and man, comprises sodium isocitrate, nicotinamide adenine dinucleotide phosphate, a source of Mg\sW\sW ions, 2:6-dichloroindophenol, phenazine, methosulphate and a triethanolamine buffer of pH 6.8-8.5. In the presence of the dinucleotide and Mg\sW\sW, isocitrate dehydrogenase catalyses the conversion of isocitrate to a -ketoglutarate and CO2: the dye-stuff system (2:6-dichloroindophenol and phenazine methosulphate) is then reduced by the resultant reduced form of the dinucleotide. The materials may be put up as a test pack in the form of the following aqueous solutions: (1) triethanolamine buffer, nicotinamide adenine dinucleotide phosphate, dichloroindophenol, MgCl2, trisodium isocitrate, (2) as (1) but omitting the citrate and (3) phenazine methosulphate. Solution (2) is used with (3) as a control. Alternatively the composition may be impregnated in a suitable carrier e.g. filter paper strips, and the product lyophilised.
GB3754665A 1965-09-02 1965-09-02 Composition for the determination of isocitrate dehydrogenase activity Expired GB1070603A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB3754665A GB1070603A (en) 1965-09-02 1965-09-02 Composition for the determination of isocitrate dehydrogenase activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3754665A GB1070603A (en) 1965-09-02 1965-09-02 Composition for the determination of isocitrate dehydrogenase activity

Publications (1)

Publication Number Publication Date
GB1070603A true GB1070603A (en) 1967-06-01

Family

ID=10397283

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3754665A Expired GB1070603A (en) 1965-09-02 1965-09-02 Composition for the determination of isocitrate dehydrogenase activity

Country Status (1)

Country Link
GB (1) GB1070603A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0199363A1 (en) * 1985-04-26 1986-10-29 Oriental Yeast Co., Ltd. Method of terminating isocitrate dehydrogenase reaction
US5258286A (en) * 1985-07-02 1993-11-02 Oriental Yeast Co., Ltd. Method of terminating isocitrate dehydrogenase reaction

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0199363A1 (en) * 1985-04-26 1986-10-29 Oriental Yeast Co., Ltd. Method of terminating isocitrate dehydrogenase reaction
US5258286A (en) * 1985-07-02 1993-11-02 Oriental Yeast Co., Ltd. Method of terminating isocitrate dehydrogenase reaction

Similar Documents

Publication Publication Date Title
GB1373562A (en) Process for the conversion of glucose into gluconic acid
ES471845A1 (en) Enzymatic synthesis of L-carnitine
GB1391876A (en) Cholesterol oxidase
GB1527243A (en) Determination of isoenzymes
JPS5596455A (en) Preserving agent for urine sample
GB1318568A (en) Colourimetric assay of dehydrogenases
GB1381662A (en) Detecting hydrogen peroxide and peroxidative compounds
GB883744A (en) Diagnostic composition
GB1535233A (en) Method for preventing aerobic decomposition processes in fermentated fodder
GB1395127A (en) Process for the quantitative photometric determination of uric acid
GB1070603A (en) Composition for the determination of isocitrate dehydrogenase activity
GB1435877A (en) Composition and test strip for detecting homocystinuria and cystinuria
GB1492530A (en) Process for keeping constant the activity of alkaline phosphatase
GB1423949A (en) Lysis of yeast cell walls
GB1275944A (en) DETERMINATION OF THE alpha-AMYLASE ACTIVITY OF A MATERIAL
GB1070157A (en) Compositions for the determination of aldolase activity
GB1267105A (en)
GB1202120A (en) A method of preparing comenic acid
GB1070266A (en) Composition for the determination of transaminase activities
GB1509539A (en) Whole blood standards for use in haematology
GB1122419A (en) Agent for the determination of ª‡-hydroxybutyrate-dehydrogenase activity
GB1507252A (en) Process for the preparation of diacetone-2-ketogulonic acid
GB874366A (en) Improvements in or relating to the treatment of steel surfaces
Chow et al. Gluconeogenic dependence on ketogenesis in isolated sheep hepatocytes
GB1462013A (en) Process for the preparation of trimethylbenzoquinone