GB1070266A - Composition for the determination of transaminase activities - Google Patents
Composition for the determination of transaminase activitiesInfo
- Publication number
- GB1070266A GB1070266A GB3296365A GB3296365A GB1070266A GB 1070266 A GB1070266 A GB 1070266A GB 3296365 A GB3296365 A GB 3296365A GB 3296365 A GB3296365 A GB 3296365A GB 1070266 A GB1070266 A GB 1070266A
- Authority
- GB
- United Kingdom
- Prior art keywords
- transaminase
- dichloroindophenol
- dinucleotide
- glutamic
- solutions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108090000340 Transaminases Proteins 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 3
- 102000003929 Transaminases Human genes 0.000 title 1
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 abstract 3
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 abstract 3
- 102000016901 Glutamate dehydrogenase Human genes 0.000 abstract 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 3
- 239000000243 solution Substances 0.000 abstract 3
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 abstract 2
- 108010082126 Alanine transaminase Proteins 0.000 abstract 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 2
- FBWADIKARMIWNM-UHFFFAOYSA-N N-3,5-dichloro-4-hydroxyphenyl-1,4-benzoquinone imine Chemical compound C1=C(Cl)C(O)=C(Cl)C=C1N=C1C=CC(=O)C=C1 FBWADIKARMIWNM-UHFFFAOYSA-N 0.000 abstract 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract 2
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 abstract 2
- 235000004279 alanine Nutrition 0.000 abstract 2
- 229940009098 aspartate Drugs 0.000 abstract 2
- 229950006238 nadide Drugs 0.000 abstract 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 2
- 102000014898 transaminase activity proteins Human genes 0.000 abstract 2
- 101710205573 Alanine aminotransferase Proteins 0.000 abstract 1
- 101710092506 Aspartate aminotransferase Proteins 0.000 abstract 1
- 101710203251 Aspartate aminotransferase 1 Proteins 0.000 abstract 1
- 101710200994 Aspartate aminotransferase, cytoplasmic Proteins 0.000 abstract 1
- 102100036608 Aspartate aminotransferase, cytoplasmic Human genes 0.000 abstract 1
- 101710201058 Aspartate aminotransferase, mitochondrial Proteins 0.000 abstract 1
- 101710192252 Aspartate/prephenate aminotransferase Proteins 0.000 abstract 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 101710101107 Probable aspartate aminotransferase Proteins 0.000 abstract 1
- 101710100249 Probable aspartate/prephenate aminotransferase Proteins 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 239000010839 body fluid Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000000975 dye Substances 0.000 abstract 1
- 229930195712 glutamate Natural products 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A composition for the determination of glutamic oxalacetic transaminase and glutamic pyruvic transaminase e.g. in the body fluids of animals or man comprises an a -keto-glutarate together with an aspartate (for glutamic oxalacetic transaminase) or with alanine (for glutamic pyruvic transaminase), glutamate dehydrogenase, nicotinamide adenine dinucleotide, 2:6-dichloroindophenol, phenazine methosulphate and a triethanolamine buffer of pH 7-8.5. In the presence of the transaminase a glutamate is formed from the first two components, and this is then oxidised to a -ketoglutarate by the glutamate dehydrogenase in the presence of, and with consequent reduction of, the dinucleotide: the dyestuff system (dichloroindophenol, phenazine methosulphate) is then reduced by the reduced dinucleotide. The materials may be separately packed in the form of the following aqueous solutions: (1) and (2) triethanolamine buffer, nicotinamide adenine dinucleotide, dichloroindophenol, aspartate or alanine (3) phenazine methosulphate, (4) glutamate dehydrogenase stabilised with glycerol and (5) a -ketoglutarate. Solutions 1, 3, 4 and 5 comprise the test reagent, solutions 2 (which has different proportions to solution 1) and 3 are used as a control. Alternatively the compositions may be impregnated into a suitable carrier, e.g. filter paper strips, and the product lyophilised.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB3296365A GB1070266A (en) | 1965-08-02 | 1965-08-02 | Composition for the determination of transaminase activities |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB3296365A GB1070266A (en) | 1965-08-02 | 1965-08-02 | Composition for the determination of transaminase activities |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1070266A true GB1070266A (en) | 1967-06-01 |
Family
ID=10346612
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB3296365A Expired GB1070266A (en) | 1965-08-02 | 1965-08-02 | Composition for the determination of transaminase activities |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1070266A (en) |
-
1965
- 1965-08-02 GB GB3296365A patent/GB1070266A/en not_active Expired
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