FR2685004A1 - Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique. - Google Patents

Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique. Download PDF

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Publication number
FR2685004A1
FR2685004A1 FR9115389A FR9115389A FR2685004A1 FR 2685004 A1 FR2685004 A1 FR 2685004A1 FR 9115389 A FR9115389 A FR 9115389A FR 9115389 A FR9115389 A FR 9115389A FR 2685004 A1 FR2685004 A1 FR 2685004A1
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FR
France
Prior art keywords
rag
vectors
sequences
genes
derivatives
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR9115389A
Other languages
English (en)
French (fr)
Other versions
FR2685004B1 (https=
Inventor
Kallenbach Sacha
Doyen Noelle
Rougeon Francois
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur
Original Assignee
Institut Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR9115389A priority Critical patent/FR2685004A1/fr
Application filed by Institut Pasteur filed Critical Institut Pasteur
Priority to DK93902325T priority patent/DK0618965T3/da
Priority to CA002125192A priority patent/CA2125192A1/fr
Priority to JP5510674A priority patent/JPH07504086A/ja
Priority to DE69233612T priority patent/DE69233612T2/de
Priority to AT93902325T priority patent/ATE322543T1/de
Priority to ES93902325T priority patent/ES2260753T3/es
Priority to EP93902325A priority patent/EP0618965B1/fr
Priority to US08/244,378 priority patent/US5756323A/en
Priority to PT93902325T priority patent/PT618965E/pt
Priority to PCT/FR1992/001178 priority patent/WO1993012228A1/fr
Publication of FR2685004A1 publication Critical patent/FR2685004A1/fr
Application granted granted Critical
Publication of FR2685004B1 publication Critical patent/FR2685004B1/fr
Granted legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
FR9115389A 1991-12-11 1991-12-11 Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique. Granted FR2685004A1 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
FR9115389A FR2685004A1 (fr) 1991-12-11 1991-12-11 Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique.
US08/244,378 US5756323A (en) 1991-12-11 1992-12-11 Method for generating structural and functional diversity in a peptide sequence
JP5510674A JPH07504086A (ja) 1991-12-11 1992-12-11 ペプチド配列の構造と機能を多様化させる方法
DE69233612T DE69233612T2 (de) 1991-12-11 1992-12-11 Verfahren zur bildung einer strukturellen und funktionellen diversität in einer peptidsequenz
AT93902325T ATE322543T1 (de) 1991-12-11 1992-12-11 Verfahren zur bildung einer strukturellen und funktionellen diversität in einer peptidsequenz
ES93902325T ES2260753T3 (es) 1991-12-11 1992-12-11 Procedimiento de generacion de una diversidad estructural y funcional en una secuencia peptidica.
DK93902325T DK0618965T3 (da) 1991-12-11 1992-12-11 Fremgangsmåde til dannelse af strukturel og funktionel diversitet i en peptidsekvens
CA002125192A CA2125192A1 (fr) 1991-12-11 1992-12-11 Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique
PT93902325T PT618965E (pt) 1991-12-11 1992-12-11 Processo de geracao de uma diversidade estrutural e funcional numa sequencia peptidica
PCT/FR1992/001178 WO1993012228A1 (fr) 1991-12-11 1992-12-11 Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique
EP93902325A EP0618965B1 (fr) 1991-12-11 1992-12-11 Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9115389A FR2685004A1 (fr) 1991-12-11 1991-12-11 Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique.

Publications (2)

Publication Number Publication Date
FR2685004A1 true FR2685004A1 (fr) 1993-06-18
FR2685004B1 FR2685004B1 (https=) 1995-01-06

Family

ID=9419935

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9115389A Granted FR2685004A1 (fr) 1991-12-11 1991-12-11 Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique.

Country Status (11)

Country Link
US (1) US5756323A (https=)
EP (1) EP0618965B1 (https=)
JP (1) JPH07504086A (https=)
AT (1) ATE322543T1 (https=)
CA (1) CA2125192A1 (https=)
DE (1) DE69233612T2 (https=)
DK (1) DK0618965T3 (https=)
ES (1) ES2260753T3 (https=)
FR (1) FR2685004A1 (https=)
PT (1) PT618965E (https=)
WO (1) WO1993012228A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DK1036198T3 (da) 1997-12-08 2013-01-02 California Inst Of Techn Fremgangsmåde til fremstilling af polynukleotid- og polypeptidsekvenser
US7122339B2 (en) 1998-10-09 2006-10-17 Medical Research Council Method for generating diversity
DK1117774T3 (da) * 1998-10-09 2010-05-03 Medical Res Council Fremgangsmåde til diversitetsgenerering
EP1010763A1 (en) * 1998-12-11 2000-06-21 Institut Pasteur Enhanced expression of heterologous proteins in recombinant bacteria through reduced growth temperature and co-expression of rare tRNA's
US6977245B2 (en) 1999-04-12 2005-12-20 The United States Of America As Represented By The Department Of Health And Human Services Oligodeoxynucleotide and its use to induce an immune response
WO2005007823A2 (en) * 2003-07-11 2005-01-27 New York University Compositions, kits, and methods for stimulation of homologous recombination
BRPI0807952A2 (pt) 2007-02-20 2014-06-10 Anaptysbio Inc Sistemas de hipermutação somática
WO2009129247A2 (en) * 2008-04-14 2009-10-22 Innovative Targeting Solutions Inc. Sequence diversity generation in immunoglobulins
EP2412806B1 (en) 2010-07-28 2014-01-08 Institut Pasteur Use of terminal deoxynucleotidyl transferase for mutagenic DNA repair to generate variability, at a determined position in DNA
EP2825653A4 (en) 2012-03-14 2016-01-20 Innovative Targeting Solutions Inc PRODUCTION OF TARGETED SEQUENCE DIVERSITY IN FUSION PROTEINS
WO2013134880A1 (en) 2012-03-14 2013-09-19 Innovative Targeting Solutions Inc. Generating targeted sequence diversity in proteins

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CELL vol. 59, 22 Décembre 1989, CELL PRESS, CAMBRIDGE, NA.; pages 1035 - 1048; D.G. SCHATZ ET AL.: 'The V(D)J recombination activating gene, RAG-1' *
MOL. CELL. BIOL. vol. 7, no. 9, Septembre 1987, AM. SOC. MICROBIOL., WASHINGTON, D.C pages 3237 - 3243; N.R. LANDAU ET AL.: 'Increased frequency of N-region insertion in a murine pre-B-cell line infected with a terminal deoxynucleotidyl transferase retroviral expression vector' *
NUCL. ACID RES. vol. 14, no. 14, 25 Juillet 1986, IRL PRESS, OXFORD, ENGLAND; pages 5777 - 5792; O. KOIWAI ET AL.: 'Isolation and characterization of bovine and mouse terminal deoxynecleotidyltransferase cDNAs expressible in mammalian cells' *
NUCL. ACID RES. vol. 18, no. 22, 25 Novembre 1990, IRL PRESS, OXFORD, ENGLAND; page 6730; S. KALLENBACH ET AL.: 'A rapid test for V(D)J recombinase activity' *
SCIENCE, vol. 246, 8 Décembre 1989, AAAS, WASHINGTON, DC, US; pages 1275 - 1281; W.D. HUSE ET AL.: 'Generation of a large combinatorial library of the immunoglobolin repertoire in phage lambda' *
SCIENCE, vol. 248, 22 Juin 1990, AAAS, WASHINGTON, DC, US; pages 1517 - 1523; M.A. OETTINGER ET AL.: 'RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination' *

Also Published As

Publication number Publication date
WO1993012228A1 (fr) 1993-06-24
DE69233612T2 (de) 2007-07-05
PT618965E (pt) 2006-08-31
JPH07504086A (ja) 1995-05-11
ES2260753T3 (es) 2006-11-01
EP0618965B1 (fr) 2006-04-05
DE69233612D1 (de) 2006-05-18
EP0618965A1 (fr) 1994-10-12
FR2685004B1 (https=) 1995-01-06
CA2125192A1 (fr) 1993-06-24
ATE322543T1 (de) 2006-04-15
DK0618965T3 (da) 2006-08-14
US5756323A (en) 1998-05-26

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