FR2482620A1 - Procede de purification de solutions contenant de la levane-saccharase - Google Patents
Procede de purification de solutions contenant de la levane-saccharase Download PDFInfo
- Publication number
- FR2482620A1 FR2482620A1 FR8011079A FR8011079A FR2482620A1 FR 2482620 A1 FR2482620 A1 FR 2482620A1 FR 8011079 A FR8011079 A FR 8011079A FR 8011079 A FR8011079 A FR 8011079A FR 2482620 A1 FR2482620 A1 FR 2482620A1
- Authority
- FR
- France
- Prior art keywords
- sep
- solutions containing
- purifying solutions
- levansucrase
- ultrafiltration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000108 ultra-filtration Methods 0.000 title claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 8
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 8
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 7
- 238000000855 fermentation Methods 0.000 title claims abstract description 7
- 230000004151 fermentation Effects 0.000 title claims abstract description 7
- 238000005194 fractionation Methods 0.000 title claims abstract description 4
- 239000012535 impurity Substances 0.000 title description 2
- -1 levan saccharide Chemical class 0.000 title 1
- 108010036940 Levansucrase Proteins 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 14
- 102000007327 Protamines Human genes 0.000 claims abstract description 7
- 108010007568 Protamines Proteins 0.000 claims abstract description 7
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims abstract description 6
- 239000001054 red pigment Substances 0.000 claims abstract description 6
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 5
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 5
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 5
- 230000020477 pH reduction Effects 0.000 claims abstract description 5
- 239000006228 supernatant Substances 0.000 claims abstract description 5
- 229940048914 protamine Drugs 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims abstract description 3
- 229910021653 sulphate ion Inorganic materials 0.000 claims abstract description 3
- 230000008030 elimination Effects 0.000 claims description 3
- 238000003379 elimination reaction Methods 0.000 claims description 3
- 229950008679 protamine sulfate Drugs 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims 9
- 239000002253 acid Substances 0.000 claims 1
- 150000007513 acids Chemical class 0.000 claims 1
- 239000008055 phosphate buffer solution Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 14
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 abstract description 4
- 229930006000 Sucrose Natural products 0.000 abstract description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 4
- 239000005720 sucrose Substances 0.000 abstract description 4
- 239000008363 phosphate buffer Substances 0.000 abstract description 3
- 229920000157 polyfructose Polymers 0.000 abstract description 3
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 abstract description 2
- 229920002307 Dextran Polymers 0.000 abstract description 2
- 229930091371 Fructose Natural products 0.000 abstract description 2
- 239000005715 Fructose Substances 0.000 abstract description 2
- 229920002472 Starch Polymers 0.000 abstract description 2
- 244000309464 bull Species 0.000 abstract description 2
- 239000008107 starch Substances 0.000 abstract description 2
- 235000019698 starch Nutrition 0.000 abstract description 2
- 229920001285 xanthan gum Polymers 0.000 abstract description 2
- 229920001218 Pullulan Polymers 0.000 abstract 1
- 239000004373 Pullulan Substances 0.000 abstract 1
- 239000000049 pigment Substances 0.000 abstract 1
- 235000019423 pullulan Nutrition 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 6
- 239000002518 antifoaming agent Substances 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 235000011073 invertase Nutrition 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 229920002670 Fructan Polymers 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 238000012872 hydroxylapatite chromatography Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1055—Levansucrase (2.4.1.10)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8011079A FR2482620A1 (fr) | 1980-05-19 | 1980-05-19 | Procede de purification de solutions contenant de la levane-saccharase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8011079A FR2482620A1 (fr) | 1980-05-19 | 1980-05-19 | Procede de purification de solutions contenant de la levane-saccharase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2482620A1 true FR2482620A1 (fr) | 1981-11-20 |
| FR2482620B1 FR2482620B1 (enExample) | 1984-01-20 |
Family
ID=9242081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8011079A Granted FR2482620A1 (fr) | 1980-05-19 | 1980-05-19 | Procede de purification de solutions contenant de la levane-saccharase |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2482620A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4477568A (en) * | 1981-09-24 | 1984-10-16 | Proefstation voor Aardappelverweking-TNO en Cooperatieve Verkoop en Produktievereniging van Aardappelmeel en Derivaten AVERE B.A. | Process for the manufacture of cyclodextrin |
-
1980
- 1980-05-19 FR FR8011079A patent/FR2482620A1/fr active Granted
Non-Patent Citations (4)
| Title |
|---|
| CA1963 * |
| CA1964 * |
| CA1975 * |
| EXBK/78 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4477568A (en) * | 1981-09-24 | 1984-10-16 | Proefstation voor Aardappelverweking-TNO en Cooperatieve Verkoop en Produktievereniging van Aardappelmeel en Derivaten AVERE B.A. | Process for the manufacture of cyclodextrin |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2482620B1 (enExample) | 1984-01-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| CD | Change of name or company name |