FR2472015A1 - Enzymes immobilisees sur un support solide - Google Patents
Enzymes immobilisees sur un support solide Download PDFInfo
- Publication number
- FR2472015A1 FR2472015A1 FR7931382A FR7931382A FR2472015A1 FR 2472015 A1 FR2472015 A1 FR 2472015A1 FR 7931382 A FR7931382 A FR 7931382A FR 7931382 A FR7931382 A FR 7931382A FR 2472015 A1 FR2472015 A1 FR 2472015A1
- Authority
- FR
- France
- Prior art keywords
- enzyme
- support
- lignin
- process according
- invertase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 39
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 39
- 239000007787 solid Substances 0.000 title abstract description 4
- 238000000034 method Methods 0.000 claims abstract description 29
- 229920005610 lignin Polymers 0.000 claims abstract description 20
- 230000008569 process Effects 0.000 claims abstract description 19
- 239000001913 cellulose Substances 0.000 claims abstract description 18
- 229920002678 cellulose Polymers 0.000 claims abstract description 18
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 15
- 230000004913 activation Effects 0.000 claims abstract description 13
- 150000004985 diamines Chemical class 0.000 claims abstract description 11
- 239000007800 oxidant agent Substances 0.000 claims abstract description 3
- 230000006641 stabilisation Effects 0.000 claims abstract description 3
- 238000011105 stabilization Methods 0.000 claims abstract description 3
- 239000012736 aqueous medium Substances 0.000 claims abstract 2
- 229940088598 enzyme Drugs 0.000 claims description 38
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 33
- 235000011073 invertase Nutrition 0.000 claims description 33
- 239000001573 invertase Substances 0.000 claims description 31
- 230000003647 oxidation Effects 0.000 claims description 19
- 238000007254 oxidation reaction Methods 0.000 claims description 19
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical group [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 claims description 19
- 240000008042 Zea mays Species 0.000 claims description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 13
- 239000002609 medium Substances 0.000 claims description 13
- 230000009467 reduction Effects 0.000 claims description 13
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 9
- 235000009973 maize Nutrition 0.000 claims description 9
- 238000005576 amination reaction Methods 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 7
- 239000003638 chemical reducing agent Substances 0.000 claims description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 6
- 102100026189 Beta-galactosidase Human genes 0.000 claims description 4
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 claims description 4
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims description 4
- 108010059881 Lactase Proteins 0.000 claims description 4
- 108010036940 Levansucrase Proteins 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- -1 cyanoborohydride Chemical compound 0.000 claims description 4
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims description 4
- 235000011180 diphosphates Nutrition 0.000 claims description 4
- 229940116108 lactase Drugs 0.000 claims description 4
- 229920002307 Dextran Polymers 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- 125000003277 amino group Chemical group 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 150000001299 aldehydes Chemical class 0.000 claims description 2
- 238000009833 condensation Methods 0.000 claims description 2
- 230000005494 condensation Effects 0.000 claims description 2
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 230000001590 oxidative effect Effects 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 241000427324 Glinus Species 0.000 claims 5
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 1
- 150000001768 cations Chemical class 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 claims 1
- 235000000346 sugar Nutrition 0.000 abstract description 9
- 150000001412 amines Chemical group 0.000 abstract description 2
- 229930006000 Sucrose Natural products 0.000 description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 17
- 230000000694 effects Effects 0.000 description 17
- 239000005720 sucrose Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 11
- 150000008163 sugars Chemical class 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008351 acetate buffer Substances 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 229930091371 Fructose Natural products 0.000 description 4
- 239000005715 Fructose Substances 0.000 description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 239000012279 sodium borohydride Substances 0.000 description 4
- 229910000033 sodium borohydride Inorganic materials 0.000 description 4
- 239000006188 syrup Substances 0.000 description 4
- 235000020357 syrup Nutrition 0.000 description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- QPXWUAQRJLSJRT-UHFFFAOYSA-N diethoxyphosphinothioyl diethyl phosphate Chemical compound CCOP(=O)(OCC)OP(=S)(OCC)OCC QPXWUAQRJLSJRT-UHFFFAOYSA-N 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- BEOOHQFXGBMRKU-UHFFFAOYSA-N sodium cyanoborohydride Chemical compound [Na+].[B-]C#N BEOOHQFXGBMRKU-UHFFFAOYSA-N 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 241001502129 Mullus Species 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 239000003082 abrasive agent Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000004984 aromatic diamines Chemical class 0.000 description 1
- 150000001540 azides Chemical class 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000004696 coordination complex Chemical class 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 108010042194 dextransucrase Proteins 0.000 description 1
- ZZTCPWRAHWXWCH-UHFFFAOYSA-N diphenylmethanediamine Chemical compound C=1C=CC=CC=1C(N)(N)C1=CC=CC=C1 ZZTCPWRAHWXWCH-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 229910001651 emery Inorganic materials 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000006266 etherification reaction Methods 0.000 description 1
- 125000003916 ethylene diamine group Chemical group 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000026030 halogenation Effects 0.000 description 1
- 238000005658 halogenation reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910001510 metal chloride Inorganic materials 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- NALMPLUMOWIVJC-UHFFFAOYSA-N n,n,4-trimethylbenzeneamine oxide Chemical compound CC1=CC=C([N+](C)(C)[O-])C=C1 NALMPLUMOWIVJC-UHFFFAOYSA-N 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- KHIWWQKSHDUIBK-UHFFFAOYSA-M periodate Chemical compound [O-]I(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-M 0.000 description 1
- 229920000157 polyfructose Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 description 1
- 229960001860 salicylate Drugs 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000011697 sodium iodate Substances 0.000 description 1
- 229940032753 sodium iodate Drugs 0.000 description 1
- 235000015281 sodium iodate Nutrition 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- HPGGPRDJHPYFRM-UHFFFAOYSA-J tin(iv) chloride Chemical compound Cl[Sn](Cl)(Cl)Cl HPGGPRDJHPYFRM-UHFFFAOYSA-J 0.000 description 1
- XJDNKRIXUMDJCW-UHFFFAOYSA-J titanium tetrachloride Chemical compound Cl[Ti](Cl)(Cl)Cl XJDNKRIXUMDJCW-UHFFFAOYSA-J 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K3/00—Invert sugar; Separation of glucose or fructose from invert sugar
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7931382A FR2472015A1 (fr) | 1979-12-21 | 1979-12-21 | Enzymes immobilisees sur un support solide |
| PCT/FR1980/000185 WO1981001860A1 (fr) | 1979-12-21 | 1980-12-18 | Enzymes immobilisees sur un support solide |
| DE8181900065T DE3066663D1 (en) | 1979-12-21 | 1980-12-18 | Immobilized enzymes on a solid carrier |
| EP81900065A EP0042395B1 (fr) | 1979-12-21 | 1980-12-18 | Enzymes immobilisees sur un support solide |
| AT81900065T ATE6272T1 (de) | 1979-12-21 | 1980-12-18 | Enzyme die an einen festen traeger gebunden sind. |
| US06/290,804 US4405715A (en) | 1979-12-21 | 1981-08-03 | Enzymes immobilized on a solid support containing cellulose and lignin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7931382A FR2472015A1 (fr) | 1979-12-21 | 1979-12-21 | Enzymes immobilisees sur un support solide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2472015A1 true FR2472015A1 (fr) | 1981-06-26 |
| FR2472015B1 FR2472015B1 (enExample) | 1985-05-24 |
Family
ID=9233052
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7931382A Granted FR2472015A1 (fr) | 1979-12-21 | 1979-12-21 | Enzymes immobilisees sur un support solide |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4405715A (enExample) |
| EP (1) | EP0042395B1 (enExample) |
| AT (1) | ATE6272T1 (enExample) |
| DE (1) | DE3066663D1 (enExample) |
| FR (1) | FR2472015A1 (enExample) |
| WO (1) | WO1981001860A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990012873A1 (en) * | 1989-04-17 | 1990-11-01 | Davy Research And Development Limited | Micro-particules for immobilising macromolecules |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1218348B (it) * | 1983-05-13 | 1990-04-12 | Farmaceutico Lofarma S A S Ora | Metodo per legare in modo stabile antigenti e allergeni a supporti solidi |
| FR2547593B1 (fr) * | 1983-06-15 | 1985-11-22 | Beghin Say Sa | Procede d'obtention de saccharose phosphorylase, saccharose phosphorylase obtenue par le procede et application a la synthese de l'acide glucose-1-phosphorique et de ses sels |
| US4861721A (en) * | 1984-09-25 | 1989-08-29 | Research Corporation | Bacteria for cellulose digestion |
| FR2573772B1 (fr) * | 1984-11-23 | 1987-03-20 | Sucre Rech & Dev | Enzyme invertase insolubilisee a haute activite specifique, son procede d'obtention et procede utilisant cet enzyme |
| FI88933C (fi) * | 1990-10-15 | 1993-07-26 | Xyrofin Oy | Foerfarande foer produktion av glukos och fruktos av sackaros |
| JP2001525367A (ja) | 1997-12-11 | 2001-12-11 | ザ・チャンセラー・マスターズ・アンド・スカラーズ・オブ・ザ・ユニバーシティ・オブ・オックスフォード | 膜関連ウイルス複製の阻害 |
| WO2001027626A2 (en) * | 1999-10-01 | 2001-04-19 | Biopreventive Ltd. | Means and system for carrying out immunoassays |
| FR2818662B1 (fr) * | 2000-12-22 | 2003-09-19 | Commissariat Energie Atomique | Procede d'immobilisation de sondes, en particulier pour realiser des puces biologiques |
| US7435564B2 (en) * | 2003-09-29 | 2008-10-14 | Instituto Technologico Y De Estudios Superiores De Monterrey | Production of invert syrup from sugarcane juice using immobilized invertase |
| US8236535B2 (en) | 2008-04-30 | 2012-08-07 | Xyleco, Inc. | Processing biomass |
| WO2010080531A1 (en) * | 2008-12-17 | 2010-07-15 | Novozymes North America, Inc. | Methods for enzymatic modification |
| KR101735543B1 (ko) * | 2009-05-20 | 2017-05-15 | 질레코 인코포레이티드 | 바이오매스의 가공처리방법 |
| US9829882B2 (en) | 2013-12-20 | 2017-11-28 | Sphero, Inc. | Self-propelled device with center of mass drive system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2247472A1 (enExample) * | 1973-10-12 | 1975-05-09 | Aquitaine Petrole | |
| US3947352A (en) * | 1974-05-31 | 1976-03-30 | Pedro Cuatrecasas | Polysaccharide matrices for use as adsorbents in affinity chromatography techniques |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3669841A (en) * | 1970-02-11 | 1972-06-13 | Monsanto Co | Attachment of enzymes to siliceous materials |
| US3706633A (en) * | 1970-04-10 | 1972-12-19 | Monsanto Co | Preparation of water-insoluble enzyme derivatives |
| GB1346631A (en) * | 1971-02-10 | 1974-02-13 | Ranks Hovis Mcdougall Ltd | Enzymes |
| US4013514A (en) * | 1972-01-04 | 1977-03-22 | Monsanto Company | Preparing a reactor containing enzymes attached to dialdehyde cellulose |
| US3741871A (en) * | 1972-01-04 | 1973-06-26 | Monsanto Co | Preparation of immobilized enzymes |
| US4279998A (en) * | 1976-06-17 | 1981-07-21 | The Board Of Regents Of The University Of Nebraska | Regenerable insoluble support for protein immobilization |
-
1979
- 1979-12-21 FR FR7931382A patent/FR2472015A1/fr active Granted
-
1980
- 1980-12-18 WO PCT/FR1980/000185 patent/WO1981001860A1/fr not_active Ceased
- 1980-12-18 EP EP81900065A patent/EP0042395B1/fr not_active Expired
- 1980-12-18 AT AT81900065T patent/ATE6272T1/de active
- 1980-12-18 DE DE8181900065T patent/DE3066663D1/de not_active Expired
-
1981
- 1981-08-03 US US06/290,804 patent/US4405715A/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2247472A1 (enExample) * | 1973-10-12 | 1975-05-09 | Aquitaine Petrole | |
| US3947352A (en) * | 1974-05-31 | 1976-03-30 | Pedro Cuatrecasas | Polysaccharide matrices for use as adsorbents in affinity chromatography techniques |
Non-Patent Citations (1)
| Title |
|---|
| EXBK/74 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990012873A1 (en) * | 1989-04-17 | 1990-11-01 | Davy Research And Development Limited | Micro-particules for immobilising macromolecules |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3066663D1 (en) | 1984-03-22 |
| EP0042395A1 (fr) | 1981-12-30 |
| WO1981001860A1 (fr) | 1981-07-09 |
| US4405715A (en) | 1983-09-20 |
| FR2472015B1 (enExample) | 1985-05-24 |
| EP0042395B1 (fr) | 1984-02-15 |
| ATE6272T1 (de) | 1984-03-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FR2472015A1 (fr) | Enzymes immobilisees sur un support solide | |
| DE2703615C2 (enExample) | ||
| JPS60232090A (ja) | 固定化酵素、その製造方法およびその使用方法 | |
| JPS6016238B2 (ja) | キシロ−スの製法 | |
| EP0676145A1 (fr) | Hydrolyse de café avec une bêta-mannanase immobilisée | |
| JPH02291265A (ja) | 水―不溶性グルコースイソメラーゼ結晶およびその製造法 | |
| Romero-Fernández et al. | Preparation of a robust immobilized biocatalyst of β-1, 4-endoxylanase by surface coating with polymers for production of xylooligosaccharides from different xylan sources | |
| EP0271289A2 (en) | Method for immobilization of enzyme and immobilized enzymes | |
| CH653363A5 (fr) | Procede de preparation de biocatalyseurs doues d'activite enzymatique et produits obtenus. | |
| FR2534272A1 (fr) | Procede de production d'une preparation d'enzyme immobilisee au moyen d'un agent de reticulation, preparation d'enzyme immobilisee ainsi obtenue et utilisation de celle-ci | |
| Lenders et al. | Immobilization of a soluble chemically thermostabilized enzyme | |
| Spagna et al. | Immobilization of the glycosidases: α-l-arabinofuranosidase and β-d-glucopyranosidase from Aspergillus niger on a chitosan derivative to increase the aroma of wine. Part II | |
| FR2553790A1 (fr) | Procede pour l'isomerisation enzymatique du glucose en fructose | |
| Stults et al. | Immobilization of proteins on partially hydrolyzed agarose beads | |
| US7312056B2 (en) | Enhancement of enzyme activity through purification and immobilization | |
| Veljković et al. | Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides | |
| JP2721968B2 (ja) | 新規酵素およびゲンチオオリゴ糖高含有シラップの製造方法 | |
| Kochanė et al. | Starch hydrolysis using maltogenase immobilized via different techniques | |
| US3833555A (en) | Polysaccharide cyclic carbamate containing compounds | |
| EP0231668A1 (fr) | Enzyme invertase insolubilisée à haute activité spécifique, son procédé d'obtention et procédé utilisant cet enzyme | |
| Nsude et al. | Immobilization and Characteristics of Trichoderma longibrachiatum Pectinase on Periwinkle (Tympanotonos fuscatus) Shell-Derived Chitosan Support | |
| RU2054481C1 (ru) | Способ получения иммобилизованных ферментов | |
| El-Shora et al. | Purification, thermostability and chemical modification of pullulanase from sunflower | |
| JPH057999B2 (enExample) | ||
| SU735594A1 (ru) | Способ получени иммобилизованной эндонуклеазы |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| CD | Change of name or company name |