FR2460481A1 - Nouveau reactif de diagnostic des parasitoses, procede de preparation de ce nouveau reactif et procede de diagnostic des parasitoses a l'aide dudit reactif - Google Patents
Nouveau reactif de diagnostic des parasitoses, procede de preparation de ce nouveau reactif et procede de diagnostic des parasitoses a l'aide dudit reactif Download PDFInfo
- Publication number
- FR2460481A1 FR2460481A1 FR7916772A FR7916772A FR2460481A1 FR 2460481 A1 FR2460481 A1 FR 2460481A1 FR 7916772 A FR7916772 A FR 7916772A FR 7916772 A FR7916772 A FR 7916772A FR 2460481 A1 FR2460481 A1 FR 2460481A1
- Authority
- FR
- France
- Prior art keywords
- wells
- buffer
- parasitosis
- antigen
- liter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000030852 Parasitic disease Diseases 0.000 title claims abstract description 29
- 239000000427 antigen Substances 0.000 title claims description 43
- 108091007433 antigens Proteins 0.000 title claims description 43
- 102000036639 antigens Human genes 0.000 title claims description 43
- 210000003651 basophil Anatomy 0.000 title claims description 27
- 238000006243 chemical reaction Methods 0.000 title description 7
- 206010020751 Hypersensitivity Diseases 0.000 title description 4
- 230000007815 allergy Effects 0.000 title description 4
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 23
- 238000003745 diagnosis Methods 0.000 claims abstract description 18
- 239000000725 suspension Substances 0.000 claims abstract description 14
- 210000004369 blood Anatomy 0.000 claims abstract description 12
- 239000008280 blood Substances 0.000 claims abstract description 12
- 241000282414 Homo sapiens Species 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims description 23
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- 210000004027 cell Anatomy 0.000 claims description 21
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 15
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 12
- 238000005119 centrifugation Methods 0.000 claims description 11
- 238000010186 staining Methods 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000002405 diagnostic procedure Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 229950003937 tolonium Drugs 0.000 claims description 8
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 claims description 8
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 7
- 230000003458 metachromatic effect Effects 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 229910001868 water Inorganic materials 0.000 claims description 7
- 229960001927 cetylpyridinium chloride Drugs 0.000 claims description 6
- YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 claims description 6
- BUACSMWVFUNQET-UHFFFAOYSA-H dialuminum;trisulfate;hydrate Chemical compound O.[Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O BUACSMWVFUNQET-UHFFFAOYSA-H 0.000 claims description 5
- 239000008188 pellet Substances 0.000 claims description 5
- 229940043517 specific immunoglobulins Drugs 0.000 claims description 5
- 230000001066 destructive effect Effects 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 210000000265 leukocyte Anatomy 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 claims description 3
- 230000003071 parasitic effect Effects 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 230000002378 acidificating effect Effects 0.000 claims description 2
- 210000004395 cytoplasmic granule Anatomy 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 210000004698 lymphocyte Anatomy 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 235000014066 European mistletoe Nutrition 0.000 claims 1
- 235000012300 Rhipsalis cassutha Nutrition 0.000 claims 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
- 241000221012 Viscum Species 0.000 claims 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims 1
- 229910052782 aluminium Inorganic materials 0.000 claims 1
- 239000003086 colorant Substances 0.000 claims 1
- 239000000032 diagnostic agent Substances 0.000 claims 1
- 229910021653 sulphate ion Inorganic materials 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 239000000969 carrier Substances 0.000 abstract description 2
- 208000009366 Echinococcosis Diseases 0.000 description 10
- 230000035931 haemagglutination Effects 0.000 description 8
- 206010014096 Echinococciasis Diseases 0.000 description 6
- 239000006285 cell suspension Substances 0.000 description 6
- 210000003743 erythrocyte Anatomy 0.000 description 5
- 238000000760 immunoelectrophoresis Methods 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 201000003808 Cystic echinococcosis Diseases 0.000 description 4
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 230000001900 immune effect Effects 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 208000026935 allergic disease Diseases 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 229920006051 Capron® Polymers 0.000 description 2
- 229920001917 Ficoll Polymers 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002390 adhesive tape Substances 0.000 description 2
- 230000004520 agglutination Effects 0.000 description 2
- 239000003708 ampul Substances 0.000 description 2
- JBKVHLHDHHXQEQ-UHFFFAOYSA-N epsilon-caprolactam Chemical compound O=C1CCCCCN1 JBKVHLHDHHXQEQ-UHFFFAOYSA-N 0.000 description 2
- 229960001340 histamine Drugs 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000002980 postoperative effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 201000004409 schistosomiasis Diseases 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000242722 Cestoda Species 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 201000006353 Filariasis Diseases 0.000 description 1
- 208000006968 Helminthiasis Diseases 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 241000242683 Schistosoma haematobium Species 0.000 description 1
- 241000242680 Schistosoma mansoni Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- -1 aluminum sulphate cetylpyridinium chloride Chemical compound 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 201000009361 ascariasis Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 208000010216 atopic IgE responsiveness Diseases 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 244000000013 helminth Species 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000009593 lumbar puncture Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 208000014837 parasitic helminthiasis infectious disease Diseases 0.000 description 1
- 230000003108 parasitologic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229950000550 sodium metrizoate Drugs 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7916772A FR2460481A1 (fr) | 1979-06-28 | 1979-06-28 | Nouveau reactif de diagnostic des parasitoses, procede de preparation de ce nouveau reactif et procede de diagnostic des parasitoses a l'aide dudit reactif |
| DE8080400959T DE3071693D1 (en) | 1979-06-28 | 1980-06-26 | Reagent for detecting parasitoses and allergies, detection process using this reagent and test kit therefor |
| DK276480A DK276480A (da) | 1979-06-28 | 1980-06-26 | Diagnostisk reagens for parasitose og allergi fremgangsmaade til fremstilling og til anvendelse deraf |
| CA000354881A CA1166566A (fr) | 1979-06-28 | 1980-06-26 | Reactif de diagnostic des parasitoses et des allergies, procede de preparation de ce nouveau reactif et procede de diagnostic des parasitoses et des allergies a l'aide dudit |
| EP80400959A EP0022698B1 (fr) | 1979-06-28 | 1980-06-26 | Réactif de diagnostic des parasitoses et des allergies, procédé de diagnostic à l'aide dudit réactif et trousse de réactif pour ledit procédé |
| NO801936A NO801936L (no) | 1979-06-28 | 1980-06-27 | Diagnosereagens for parasitoser og allergier, fremgangsmaate for fremstilling av reagenset, samt anvendelse derav |
| OA57146A OA06560A (fr) | 1979-06-28 | 1980-06-28 | Nouveau réactif de diagnostic des parasitoses et des allergies, procédé de préparation de ce nouveau réactif et procédé de diagnostic des parasitoses et des allergies à l'aide dudit réactif. |
| ES492912A ES492912A0 (es) | 1979-06-28 | 1980-06-28 | Metodo de analisis para diagonosticar la parasitosis y alergias. |
| US06/489,603 US4640897A (en) | 1979-06-28 | 1983-05-02 | Immunoanalysis of basophil-containing blood fraction for diagnosing parasitoses and allergies |
| ES524192A ES524192A0 (es) | 1979-06-28 | 1983-07-16 | Procedimiento de preparacion de un nuevo reactivo para el diagnostico de las parasitosis y de las alergias. |
| ES530527A ES530527A0 (es) | 1979-06-28 | 1984-03-12 | Sistema de diagnostico de parasitosis y alergias |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7916772A FR2460481A1 (fr) | 1979-06-28 | 1979-06-28 | Nouveau reactif de diagnostic des parasitoses, procede de preparation de ce nouveau reactif et procede de diagnostic des parasitoses a l'aide dudit reactif |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2460481A1 true FR2460481A1 (fr) | 1981-01-23 |
| FR2460481B1 FR2460481B1 (enExample) | 1983-02-25 |
Family
ID=9227251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7916772A Granted FR2460481A1 (fr) | 1979-06-28 | 1979-06-28 | Nouveau reactif de diagnostic des parasitoses, procede de preparation de ce nouveau reactif et procede de diagnostic des parasitoses a l'aide dudit reactif |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2460481A1 (enExample) |
-
1979
- 1979-06-28 FR FR7916772A patent/FR2460481A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| FR2460481B1 (enExample) | 1983-02-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |