FI95394B - Användning av pSG5 som temperaturkänslig plasmid - Google Patents
Användning av pSG5 som temperaturkänslig plasmid Download PDFInfo
- Publication number
- FI95394B FI95394B FI891335A FI891335A FI95394B FI 95394 B FI95394 B FI 95394B FI 891335 A FI891335 A FI 891335A FI 891335 A FI891335 A FI 891335A FI 95394 B FI95394 B FI 95394B
- Authority
- FI
- Finland
- Prior art keywords
- plasmid
- psg5
- marker
- mutants
- screened
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pyrrole Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Claims (6)
1. Användning av pSG5-replikonen för framställning av en temperaturkänslig hybridplasmid.
2. Användning av pSG5-replikonen för detektion av IS-element och transposoner, kännetecknad därav, att en marker insätts i en plasmid omfattande pSG5-replikonen, Streptomyces-organismer transformeras med den sä erhällna hybridplasmiden, transformanterna inkuberas 10 vid en temperatur över 36 °C, screenas pä basis av markern och DNA-omrädet, i vilket rekombination skett, analyseras.
3. Förfarande för framställning av Streptomyces-mutanter, kännetecknat därav, att hela DNA:t isoleras frän en Streptomyces-utg&ngsstam, det omvandlas 15 till korta fragment, dessa integreras i en hybridplasmid som omfattar markern samt pSG5-replikonen, den erhällna hybridplasmidpopulationen transformeras i utgängsstammen, transformanterna screenas under användning av pä markern ·' baserad screening, hybridplasmiderna elimineras genom att 20 höja temperaturen över tröskelvärdet och mutanterna screenas under användning av upprepad pä markern baserad screening.
4. Användning av Streptomyces-mutanter framställda medelst förfarandet enligt patentkrav 3 för isolering av ·*·'' 25 muterade gener, kännetecknad därav, att mute- rade gener isoleras ur de screenade mutanterna.
5. Användning av Streptomyces-mutanter framställda medelst förfarandet enligt patentkrav 3 för isolering av gener av motsvarande vildtyp, kännetecknad 30 därav, att motsvarande vildtypsgener isoleras med hjälp av *; de muterade gener som anges i patentkrav 4.
6. Användning enligt patentkrav 5, kännetecknad därav, att hybridplasmiden som omfattar pSG5-replikonen innehäller en marker som kan screenas i E. 3. coli och att man vid isoleringen av de muterade generna använder en kosmidbank.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3809692 | 1988-03-23 | ||
| DE3809692A DE3809692A1 (de) | 1988-03-23 | 1988-03-23 | Verwendung von psg5 als temperatursensitives plasmid |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI891335A0 FI891335A0 (sv) | 1989-03-21 |
| FI891335A FI891335A (sv) | 1989-09-24 |
| FI95394B true FI95394B (sv) | 1995-10-13 |
| FI95394C FI95394C (sv) | 1996-01-25 |
Family
ID=6350421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI891335A FI95394C (sv) | 1988-03-23 | 1989-03-21 | Användning av pSG5 som temperaturkänslig plasmid |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP0334282B1 (sv) |
| JP (1) | JP2770975B2 (sv) |
| KR (1) | KR970003961B1 (sv) |
| AT (1) | ATE108485T1 (sv) |
| AU (1) | AU615524B2 (sv) |
| CA (1) | CA1335964C (sv) |
| DE (2) | DE3809692A1 (sv) |
| DK (1) | DK175475B1 (sv) |
| ES (1) | ES2056987T3 (sv) |
| FI (1) | FI95394C (sv) |
| HU (1) | HU213350B (sv) |
| IE (1) | IE63496B1 (sv) |
| IL (1) | IL89686A (sv) |
| NO (1) | NO178157C (sv) |
| PT (1) | PT90090B (sv) |
| ZA (1) | ZA892123B (sv) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3809691A1 (de) * | 1988-03-23 | 1989-10-12 | Hoechst Ag | Verfahren zur selektion grosser dna-abschnitte |
| DE4011863A1 (de) * | 1990-04-12 | 1991-10-17 | Hoechst Ag | Regulierte genexpression in streptomyceten |
| FR2688515B1 (fr) * | 1992-03-13 | 1995-03-31 | Institut Rech Agronomique | Plasmide thermosensible. |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0158872B1 (de) * | 1984-03-31 | 1989-01-18 | Hoechst Aktiengesellschaft | Das Streptomyceten-Plasmid pSG5, Verfahren zu seiner Gewinnung und seine Verwendung |
| DE3412093A1 (de) * | 1984-03-31 | 1985-10-10 | Hoechst Ag, 6230 Frankfurt | Hybridplasmide mit einem streptomyceten- und einem escherichia coli-replicon |
| DE3614310A1 (de) * | 1986-04-28 | 1987-10-29 | Hoechst Ag | Verfahren zur isolierung mutierter gene sowie der entsprechenden wildtyp-gene |
| DE3627392A1 (de) * | 1986-08-13 | 1988-04-28 | Hoechst Ag | Farbmarker fuer klonierungen in streptomyces lividans |
-
1988
- 1988-03-23 DE DE3809692A patent/DE3809692A1/de not_active Withdrawn
-
1989
- 1989-03-21 AT AT89105012T patent/ATE108485T1/de not_active IP Right Cessation
- 1989-03-21 EP EP89105012A patent/EP0334282B1/de not_active Expired - Lifetime
- 1989-03-21 FI FI891335A patent/FI95394C/sv not_active IP Right Cessation
- 1989-03-21 ES ES89105012T patent/ES2056987T3/es not_active Expired - Lifetime
- 1989-03-21 IL IL8968689A patent/IL89686A/en not_active IP Right Cessation
- 1989-03-21 ZA ZA892123A patent/ZA892123B/xx unknown
- 1989-03-21 DE DE58908022T patent/DE58908022D1/de not_active Expired - Lifetime
- 1989-03-22 HU HU891388A patent/HU213350B/hu unknown
- 1989-03-22 IE IE89889A patent/IE63496B1/en not_active IP Right Cessation
- 1989-03-22 JP JP1067773A patent/JP2770975B2/ja not_active Expired - Lifetime
- 1989-03-22 AU AU31582/89A patent/AU615524B2/en not_active Expired
- 1989-03-22 KR KR1019890003551A patent/KR970003961B1/ko not_active IP Right Cessation
- 1989-03-22 DK DK198901460A patent/DK175475B1/da not_active IP Right Cessation
- 1989-03-22 NO NO891267A patent/NO178157C/no not_active IP Right Cessation
- 1989-03-22 CA CA000594516A patent/CA1335964C/en not_active Expired - Lifetime
- 1989-03-22 PT PT90090A patent/PT90090B/pt not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DE58908022D1 (de) | 1994-08-18 |
| DE3809692A1 (de) | 1989-10-12 |
| IE63496B1 (en) | 1995-05-03 |
| NO891267D0 (no) | 1989-03-22 |
| KR970003961B1 (ko) | 1997-03-24 |
| IL89686A0 (en) | 1989-09-28 |
| CA1335964C (en) | 1995-06-20 |
| NO178157B (no) | 1995-10-23 |
| PT90090A (pt) | 1989-11-10 |
| FI95394C (sv) | 1996-01-25 |
| ATE108485T1 (de) | 1994-07-15 |
| NO891267L (no) | 1989-09-25 |
| KR890014741A (ko) | 1989-10-25 |
| ZA892123B (en) | 1989-11-29 |
| HU213350B (en) | 1997-05-28 |
| NO178157C (no) | 1996-01-31 |
| AU3158289A (en) | 1989-09-28 |
| JP2770975B2 (ja) | 1998-07-02 |
| AU615524B2 (en) | 1991-10-03 |
| DK146089A (da) | 1989-09-24 |
| JPH029376A (ja) | 1990-01-12 |
| EP0334282A2 (de) | 1989-09-27 |
| PT90090B (pt) | 1994-06-30 |
| DK146089D0 (da) | 1989-03-22 |
| FI891335A0 (sv) | 1989-03-21 |
| HUT50511A (en) | 1990-02-28 |
| FI891335A (sv) | 1989-09-24 |
| DK175475B1 (da) | 2004-11-08 |
| EP0334282B1 (de) | 1994-07-13 |
| EP0334282A3 (en) | 1989-11-29 |
| ES2056987T3 (es) | 1994-10-16 |
| IL89686A (en) | 1994-12-29 |
| IE890898L (en) | 1989-09-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Patent granted |
Owner name: HOECHST AKTIENGESELLSCHAFT |
|
| BB | Publication of examined application | ||
| FG | Patent granted |
Owner name: AVENTIS PHARMA DEUTSCHLAND GMBH |
|
| PC | Transfer of assignment of patent |
Owner name: SANOFI-AVENTIS DEUTSCHLAND GMBH Free format text: SANOFI-AVENTIS DEUTSCHLAND GMBH |
|
| MA | Patent expired |