AU3158289A - The use of psg5 as a temperature-sensitive plasmid - Google Patents

The use of psg5 as a temperature-sensitive plasmid

Info

Publication number
AU3158289A
AU3158289A AU31582/89A AU3158289A AU3158289A AU 3158289 A AU3158289 A AU 3158289A AU 31582/89 A AU31582/89 A AU 31582/89A AU 3158289 A AU3158289 A AU 3158289A AU 3158289 A AU3158289 A AU 3158289A
Authority
AU
Australia
Prior art keywords
psg5
temperature
sensitive plasmid
sensitive
plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU31582/89A
Other versions
AU615524B2 (en
Inventor
Gunter Muth
Bernhard Nussbaumer
Alfred Puhler
Wolfgang Wohlleben
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Aventis Deutschland GmbH
Original Assignee
Sanofi Aventis Deutschland GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Aventis Deutschland GmbH filed Critical Sanofi Aventis Deutschland GmbH
Publication of AU3158289A publication Critical patent/AU3158289A/en
Application granted granted Critical
Publication of AU615524B2 publication Critical patent/AU615524B2/en
Assigned to AVENTIS PHARMA DEUTSCHLAND GMBH reassignment AVENTIS PHARMA DEUTSCHLAND GMBH Alteration of Name(s) in Register under S187 Assignors: HOECHST AKTIENGESELLSCHAFT
Assigned to SANOFI-AVENTIS DEUTSCHLAND GMBH reassignment SANOFI-AVENTIS DEUTSCHLAND GMBH Request to Amend Deed and Register Assignors: AVENTIS PHARMA DEUTSCHLAND GMBH
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces

Abstract

The streptomycetes plasmid pSG5 is temperature-sensitive. It is thus possible with the aid of the pSG5 replicon to prepare temperature-sensitive hybrid plasmids. The latter are suitable for screening for DNA segments which are homologous with DNA segments of the hybrid plasmid.
AU31582/89A 1988-03-23 1989-03-22 The use of psg5 as a temperature-sensitive plasmid Expired AU615524B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3809692A DE3809692A1 (en) 1988-03-23 1988-03-23 USE OF PSG5 AS A TEMPERATURE-SENSITIVE PLASMIDE
DE3809692 1988-03-23

Publications (2)

Publication Number Publication Date
AU3158289A true AU3158289A (en) 1989-09-28
AU615524B2 AU615524B2 (en) 1991-10-03

Family

ID=6350421

Family Applications (1)

Application Number Title Priority Date Filing Date
AU31582/89A Expired AU615524B2 (en) 1988-03-23 1989-03-22 The use of psg5 as a temperature-sensitive plasmid

Country Status (16)

Country Link
EP (1) EP0334282B1 (en)
JP (1) JP2770975B2 (en)
KR (1) KR970003961B1 (en)
AT (1) ATE108485T1 (en)
AU (1) AU615524B2 (en)
CA (1) CA1335964C (en)
DE (2) DE3809692A1 (en)
DK (1) DK175475B1 (en)
ES (1) ES2056987T3 (en)
FI (1) FI95394C (en)
HU (1) HU213350B (en)
IE (1) IE63496B1 (en)
IL (1) IL89686A (en)
NO (1) NO178157C (en)
PT (1) PT90090B (en)
ZA (1) ZA892123B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU630551B2 (en) * 1990-04-12 1992-10-29 Hoechst Aktiengesellschaft Regulated gene expression in streptomycetes

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3809691A1 (en) * 1988-03-23 1989-10-12 Hoechst Ag METHOD FOR SELECTION OF LARGE DNA SECTIONS
FR2688515B1 (en) * 1992-03-13 1995-03-31 Institut Rech Agronomique THERMOSENSITIVE PLASMID.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0158872B1 (en) * 1984-03-31 1989-01-18 Hoechst Aktiengesellschaft Streptomycetes plasmid psg5, process for its preparation and its use
DE3412093A1 (en) * 1984-03-31 1985-10-10 Hoechst Ag, 6230 Frankfurt HYBRID PLASMIDE WITH A STREPTOMYCETE AND ESCHERICHIA COLI REPLICON
DE3614310A1 (en) * 1986-04-28 1987-10-29 Hoechst Ag METHOD FOR ISOLATING MUTED GENES AND THE CORRESPONDING WILD-TYPE GENES
DE3627392A1 (en) * 1986-08-13 1988-04-28 Hoechst Ag COLOR MARKER FOR CLONING IN STREPTOMYCES LIVIDANS

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU630551B2 (en) * 1990-04-12 1992-10-29 Hoechst Aktiengesellschaft Regulated gene expression in streptomycetes

Also Published As

Publication number Publication date
FI891335A (en) 1989-09-24
DE58908022D1 (en) 1994-08-18
KR970003961B1 (en) 1997-03-24
EP0334282A2 (en) 1989-09-27
CA1335964C (en) 1995-06-20
EP0334282A3 (en) 1989-11-29
DK146089A (en) 1989-09-24
JPH029376A (en) 1990-01-12
IL89686A0 (en) 1989-09-28
EP0334282B1 (en) 1994-07-13
HU213350B (en) 1997-05-28
DK146089D0 (en) 1989-03-22
PT90090A (en) 1989-11-10
ATE108485T1 (en) 1994-07-15
IL89686A (en) 1994-12-29
HUT50511A (en) 1990-02-28
DK175475B1 (en) 2004-11-08
NO891267L (en) 1989-09-25
IE890898L (en) 1989-09-23
NO178157C (en) 1996-01-31
KR890014741A (en) 1989-10-25
PT90090B (en) 1994-06-30
FI95394B (en) 1995-10-13
JP2770975B2 (en) 1998-07-02
NO891267D0 (en) 1989-03-22
FI95394C (en) 1996-01-25
FI891335A0 (en) 1989-03-21
DE3809692A1 (en) 1989-10-12
ES2056987T3 (en) 1994-10-16
IE63496B1 (en) 1995-05-03
NO178157B (en) 1995-10-23
AU615524B2 (en) 1991-10-03
ZA892123B (en) 1989-11-29

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Legal Events

Date Code Title Description
PC Assignment registered

Owner name: AVENTIS PHARMA DEUTSCHLAND GMBH

Free format text: FORMER OWNER WAS: HOECHST AKTIENGESELLSCHAFT