FI891335A0 - Use of pSG5 as a temperature sensitive plasmid - Google Patents

Use of pSG5 as a temperature sensitive plasmid

Info

Publication number
FI891335A0
FI891335A0 FI891335A FI891335A FI891335A0 FI 891335 A0 FI891335 A0 FI 891335A0 FI 891335 A FI891335 A FI 891335A FI 891335 A FI891335 A FI 891335A FI 891335 A0 FI891335 A0 FI 891335A0
Authority
FI
Finland
Prior art keywords
psg5
temperature sensitive
sensitive plasmid
plasmid
temperature
Prior art date
Application number
FI891335A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI891335A (en
FI95394B (en
FI95394C (en
Inventor
Wolfgang Wohlleben
Guenter Muth
Alfred Puehler
Bernhard Nussbaumer
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of FI891335A0 publication Critical patent/FI891335A0/en
Publication of FI891335A publication Critical patent/FI891335A/en
Publication of FI95394B publication Critical patent/FI95394B/en
Application granted granted Critical
Publication of FI95394C publication Critical patent/FI95394C/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces

Abstract

The streptomycetes plasmid pSG5 is temperature-sensitive. It is thus possible with the aid of the pSG5 replicon to prepare temperature-sensitive hybrid plasmids. The latter are suitable for screening for DNA segments which are homologous with DNA segments of the hybrid plasmid.
FI891335A 1988-03-23 1989-03-21 Use of pSG5 as a temperature sensitive plasmid FI95394C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3809692A DE3809692A1 (en) 1988-03-23 1988-03-23 USE OF PSG5 AS A TEMPERATURE-SENSITIVE PLASMIDE
DE3809692 1988-03-23

Publications (4)

Publication Number Publication Date
FI891335A0 true FI891335A0 (en) 1989-03-21
FI891335A FI891335A (en) 1989-09-24
FI95394B FI95394B (en) 1995-10-13
FI95394C FI95394C (en) 1996-01-25

Family

ID=6350421

Family Applications (1)

Application Number Title Priority Date Filing Date
FI891335A FI95394C (en) 1988-03-23 1989-03-21 Use of pSG5 as a temperature sensitive plasmid

Country Status (16)

Country Link
EP (1) EP0334282B1 (en)
JP (1) JP2770975B2 (en)
KR (1) KR970003961B1 (en)
AT (1) ATE108485T1 (en)
AU (1) AU615524B2 (en)
CA (1) CA1335964C (en)
DE (2) DE3809692A1 (en)
DK (1) DK175475B1 (en)
ES (1) ES2056987T3 (en)
FI (1) FI95394C (en)
HU (1) HU213350B (en)
IE (1) IE63496B1 (en)
IL (1) IL89686A (en)
NO (1) NO178157C (en)
PT (1) PT90090B (en)
ZA (1) ZA892123B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3809691A1 (en) * 1988-03-23 1989-10-12 Hoechst Ag METHOD FOR SELECTION OF LARGE DNA SECTIONS
DE4011863A1 (en) * 1990-04-12 1991-10-17 Hoechst Ag REGULATED GENE EXPRESSION IN STREPTOMYCETES
FR2688515B1 (en) * 1992-03-13 1995-03-31 Institut Rech Agronomique THERMOSENSITIVE PLASMID.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0158872B1 (en) * 1984-03-31 1989-01-18 Hoechst Aktiengesellschaft Streptomycetes plasmid psg5, process for its preparation and its use
DE3412093A1 (en) * 1984-03-31 1985-10-10 Hoechst Ag, 6230 Frankfurt HYBRID PLASMIDE WITH A STREPTOMYCETE AND ESCHERICHIA COLI REPLICON
DE3614310A1 (en) * 1986-04-28 1987-10-29 Hoechst Ag METHOD FOR ISOLATING MUTED GENES AND THE CORRESPONDING WILD-TYPE GENES
DE3627392A1 (en) * 1986-08-13 1988-04-28 Hoechst Ag COLOR MARKER FOR CLONING IN STREPTOMYCES LIVIDANS

Also Published As

Publication number Publication date
FI891335A (en) 1989-09-24
DE58908022D1 (en) 1994-08-18
KR970003961B1 (en) 1997-03-24
EP0334282A2 (en) 1989-09-27
CA1335964C (en) 1995-06-20
EP0334282A3 (en) 1989-11-29
DK146089A (en) 1989-09-24
JPH029376A (en) 1990-01-12
IL89686A0 (en) 1989-09-28
EP0334282B1 (en) 1994-07-13
HU213350B (en) 1997-05-28
DK146089D0 (en) 1989-03-22
PT90090A (en) 1989-11-10
ATE108485T1 (en) 1994-07-15
AU3158289A (en) 1989-09-28
IL89686A (en) 1994-12-29
HUT50511A (en) 1990-02-28
DK175475B1 (en) 2004-11-08
NO891267L (en) 1989-09-25
IE890898L (en) 1989-09-23
NO178157C (en) 1996-01-31
KR890014741A (en) 1989-10-25
PT90090B (en) 1994-06-30
FI95394B (en) 1995-10-13
JP2770975B2 (en) 1998-07-02
NO891267D0 (en) 1989-03-22
FI95394C (en) 1996-01-25
DE3809692A1 (en) 1989-10-12
ES2056987T3 (en) 1994-10-16
IE63496B1 (en) 1995-05-03
NO178157B (en) 1995-10-23
AU615524B2 (en) 1991-10-03
ZA892123B (en) 1989-11-29

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Legal Events

Date Code Title Description
FG Patent granted

Owner name: HOECHST AKTIENGESELLSCHAFT

BB Publication of examined application
FG Patent granted

Owner name: AVENTIS PHARMA DEUTSCHLAND GMBH

PC Transfer of assignment of patent

Owner name: SANOFI-AVENTIS DEUTSCHLAND GMBH

Free format text: SANOFI-AVENTIS DEUTSCHLAND GMBH

MA Patent expired