NO178157C - Use of pSG5 as a Temperature Sensitive Plasmid and Method for Using Hybrid Plasmid with the pSG5 Replicon for Studies of Streptomyces - Google Patents

Use of pSG5 as a Temperature Sensitive Plasmid and Method for Using Hybrid Plasmid with the pSG5 Replicon for Studies of Streptomyces

Info

Publication number
NO178157C
NO178157C NO891267A NO891267A NO178157C NO 178157 C NO178157 C NO 178157C NO 891267 A NO891267 A NO 891267A NO 891267 A NO891267 A NO 891267A NO 178157 C NO178157 C NO 178157C
Authority
NO
Norway
Prior art keywords
psg5
plasmid
streptomyces
replicon
studies
Prior art date
Application number
NO891267A
Other languages
Norwegian (no)
Other versions
NO891267L (en
NO891267D0 (en
NO178157B (en
Inventor
Wolfgang Wohlleben
Gunter Muth
Alfred Puhler
Bernhard Nussbaumer
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of NO891267D0 publication Critical patent/NO891267D0/en
Publication of NO891267L publication Critical patent/NO891267L/en
Publication of NO178157B publication Critical patent/NO178157B/en
Publication of NO178157C publication Critical patent/NO178157C/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces

Abstract

The streptomycetes plasmid pSG5 is temperature-sensitive. It is thus possible with the aid of the pSG5 replicon to prepare temperature-sensitive hybrid plasmids. The latter are suitable for screening for DNA segments which are homologous with DNA segments of the hybrid plasmid.
NO891267A 1988-03-23 1989-03-22 Use of pSG5 as a Temperature Sensitive Plasmid and Method for Using Hybrid Plasmid with the pSG5 Replicon for Studies of Streptomyces NO178157C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3809692A DE3809692A1 (en) 1988-03-23 1988-03-23 USE OF PSG5 AS A TEMPERATURE-SENSITIVE PLASMIDE

Publications (4)

Publication Number Publication Date
NO891267D0 NO891267D0 (en) 1989-03-22
NO891267L NO891267L (en) 1989-09-25
NO178157B NO178157B (en) 1995-10-23
NO178157C true NO178157C (en) 1996-01-31

Family

ID=6350421

Family Applications (1)

Application Number Title Priority Date Filing Date
NO891267A NO178157C (en) 1988-03-23 1989-03-22 Use of pSG5 as a Temperature Sensitive Plasmid and Method for Using Hybrid Plasmid with the pSG5 Replicon for Studies of Streptomyces

Country Status (16)

Country Link
EP (1) EP0334282B1 (en)
JP (1) JP2770975B2 (en)
KR (1) KR970003961B1 (en)
AT (1) ATE108485T1 (en)
AU (1) AU615524B2 (en)
CA (1) CA1335964C (en)
DE (2) DE3809692A1 (en)
DK (1) DK175475B1 (en)
ES (1) ES2056987T3 (en)
FI (1) FI95394C (en)
HU (1) HU213350B (en)
IE (1) IE63496B1 (en)
IL (1) IL89686A (en)
NO (1) NO178157C (en)
PT (1) PT90090B (en)
ZA (1) ZA892123B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3809691A1 (en) * 1988-03-23 1989-10-12 Hoechst Ag METHOD FOR SELECTION OF LARGE DNA SECTIONS
DE4011863A1 (en) * 1990-04-12 1991-10-17 Hoechst Ag REGULATED GENE EXPRESSION IN STREPTOMYCETES
FR2688515B1 (en) * 1992-03-13 1995-03-31 Institut Rech Agronomique THERMOSENSITIVE PLASMID.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0158872B1 (en) * 1984-03-31 1989-01-18 Hoechst Aktiengesellschaft Streptomycetes plasmid psg5, process for its preparation and its use
DE3412093A1 (en) * 1984-03-31 1985-10-10 Hoechst Ag, 6230 Frankfurt HYBRID PLASMIDE WITH A STREPTOMYCETE AND ESCHERICHIA COLI REPLICON
DE3614310A1 (en) * 1986-04-28 1987-10-29 Hoechst Ag METHOD FOR ISOLATING MUTED GENES AND THE CORRESPONDING WILD-TYPE GENES
DE3627392A1 (en) * 1986-08-13 1988-04-28 Hoechst Ag COLOR MARKER FOR CLONING IN STREPTOMYCES LIVIDANS

Also Published As

Publication number Publication date
FI891335A (en) 1989-09-24
DE58908022D1 (en) 1994-08-18
KR970003961B1 (en) 1997-03-24
EP0334282A2 (en) 1989-09-27
CA1335964C (en) 1995-06-20
EP0334282A3 (en) 1989-11-29
DK146089A (en) 1989-09-24
JPH029376A (en) 1990-01-12
IL89686A0 (en) 1989-09-28
EP0334282B1 (en) 1994-07-13
HU213350B (en) 1997-05-28
DK146089D0 (en) 1989-03-22
PT90090A (en) 1989-11-10
ATE108485T1 (en) 1994-07-15
AU3158289A (en) 1989-09-28
IL89686A (en) 1994-12-29
HUT50511A (en) 1990-02-28
DK175475B1 (en) 2004-11-08
NO891267L (en) 1989-09-25
IE890898L (en) 1989-09-23
KR890014741A (en) 1989-10-25
PT90090B (en) 1994-06-30
FI95394B (en) 1995-10-13
JP2770975B2 (en) 1998-07-02
NO891267D0 (en) 1989-03-22
FI95394C (en) 1996-01-25
FI891335A0 (en) 1989-03-21
DE3809692A1 (en) 1989-10-12
ES2056987T3 (en) 1994-10-16
IE63496B1 (en) 1995-05-03
NO178157B (en) 1995-10-23
AU615524B2 (en) 1991-10-03
ZA892123B (en) 1989-11-29

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Legal Events

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