DK45790D0 - PROCEDURE AND APPARATUS FOR GETTING PLASMIDS AND COSMIDES - Google Patents

PROCEDURE AND APPARATUS FOR GETTING PLASMIDS AND COSMIDES

Info

Publication number
DK45790D0
DK45790D0 DK045790A DK45790A DK45790D0 DK 45790 D0 DK45790 D0 DK 45790D0 DK 045790 A DK045790 A DK 045790A DK 45790 A DK45790 A DK 45790A DK 45790 D0 DK45790 D0 DK 45790D0
Authority
DK
Denmark
Prior art keywords
treatment chamber
plasmids
cosmides
getting
procedure
Prior art date
Application number
DK045790A
Other languages
Danish (da)
Other versions
DK45790A (en
Inventor
Daniel Cohen
Original Assignee
Bertin & Cie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bertin & Cie filed Critical Bertin & Cie
Publication of DK45790A publication Critical patent/DK45790A/en
Publication of DK45790D0 publication Critical patent/DK45790D0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material

Abstract

The invention concerns a process and an installation for obtaining whole plasmids and cosmids. The installation for obtaining whole plasmids and cosmids present in bacterial cells comprises at least one means (5) for separating the cells from their culture medium, at least one treatment chamber (4), at least one electrophoresis gel (7) which serves as an electrophoretic migration medium adjacent to, and associated with, said treatment chamber, at least one means (8) for applying an electric field which induces the electrophoretic migration, removable and/or retractable means (11) for placing said electrophoresis gel in communication with said treatment chamber, and a microprocessor for computerizing the sequential control of said separating means, said communication means and said means for applying an electric field.
DK045790A 1988-06-21 1990-02-21 PROCEDURE AND APPARATUS FOR GETTING PLASMIDS AND COSMIDES DK45790D0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8808306A FR2632970B1 (en) 1988-06-21 1988-06-21 PROCESS AND PLANT FOR OBTAINING PLASMIDS AND COSMIDES
PCT/FR1989/000301 WO1989012682A1 (en) 1988-06-21 1989-06-15 Process and installation for obtaining plasmids and cosmids

Publications (2)

Publication Number Publication Date
DK45790A DK45790A (en) 1990-02-21
DK45790D0 true DK45790D0 (en) 1990-02-21

Family

ID=9367529

Family Applications (1)

Application Number Title Priority Date Filing Date
DK045790A DK45790D0 (en) 1988-06-21 1990-02-21 PROCEDURE AND APPARATUS FOR GETTING PLASMIDS AND COSMIDES

Country Status (9)

Country Link
EP (1) EP0348275B1 (en)
JP (1) JPH03501210A (en)
AT (1) ATE101645T1 (en)
AU (1) AU3843689A (en)
DE (1) DE68913087T2 (en)
DK (1) DK45790D0 (en)
ES (1) ES2049340T3 (en)
FR (1) FR2632970B1 (en)
WO (1) WO1989012682A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
FR2742163B1 (en) * 1995-12-06 1998-03-20 Agronomique Inst Nat Rech METHOD FOR IDENTIFYING BACTERIAL SPECIES

Also Published As

Publication number Publication date
JPH03501210A (en) 1991-03-22
EP0348275B1 (en) 1994-02-16
ATE101645T1 (en) 1994-03-15
DK45790A (en) 1990-02-21
FR2632970A1 (en) 1989-12-22
WO1989012682A1 (en) 1989-12-28
DE68913087D1 (en) 1994-03-24
FR2632970B1 (en) 1990-09-28
DE68913087T2 (en) 1994-05-26
AU3843689A (en) 1990-01-12
EP0348275A1 (en) 1989-12-27
ES2049340T3 (en) 1994-04-16

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Legal Events

Date Code Title Description
AHB Application shelved due to non-payment