FI86886C - Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli - Google Patents

Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli Download PDF

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Publication number
FI86886C
FI86886C FI840918A FI840918A FI86886C FI 86886 C FI86886 C FI 86886C FI 840918 A FI840918 A FI 840918A FI 840918 A FI840918 A FI 840918A FI 86886 C FI86886 C FI 86886C
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FI
Finland
Prior art keywords
plasmid
dna
ecori
map shown
prochymosin
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Application number
FI840918A
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English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI86886B (fi
FI840918L (fi
FI840918A0 (fi
Inventor
Takeshi Uozumi
Katsuhiko Nishimori
Norio Shimizu
Yoshiyuki Kawaguchi
Makoto Hidaka
Teruhiko Beppu
Original Assignee
Teruhiko Beppu
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Application filed by Teruhiko Beppu filed Critical Teruhiko Beppu
Publication of FI840918A0 publication Critical patent/FI840918A0/fi
Publication of FI840918L publication Critical patent/FI840918L/fi
Application granted granted Critical
Publication of FI86886B publication Critical patent/FI86886B/fi
Publication of FI86886C publication Critical patent/FI86886C/fi

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6481Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/71Expression systems using regulatory sequences derived from the trp-operon
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6483Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23Aspartic endopeptidases (3.4.23)
    • C12Y304/23004Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
FI840918A 1983-03-09 1984-03-07 Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli FI86886C (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
JP3843983 1983-03-09

Publications (4)

Publication Number Publication Date
FI840918A0 FI840918A0 (fi) 1984-03-07
FI840918L FI840918L (fi) 1984-09-10
FI86886B FI86886B (fi) 1992-07-15
FI86886C true FI86886C (fi) 1992-10-26

Family

ID=12525334

Family Applications (1)

Application Number Title Priority Date Filing Date
FI840918A FI86886C (fi) 1983-03-09 1984-03-07 Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli

Country Status (9)

Country Link
EP (1) EP0121775A1 (OSRAM)
JP (1) JPS59166086A (OSRAM)
AR (1) AR241795A1 (OSRAM)
AU (1) AU572827B2 (OSRAM)
BR (1) BR8401049A (OSRAM)
DK (1) DK136184A (OSRAM)
FI (1) FI86886C (OSRAM)
IE (1) IE57264B1 (OSRAM)
NZ (1) NZ207414A (OSRAM)

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JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンcDΝAの全配列を有する新規な発現プラスミド
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Also Published As

Publication number Publication date
IE840559L (en) 1984-09-09
DK136184D0 (da) 1984-02-29
AU572827B2 (en) 1988-05-19
EP0121775A1 (en) 1984-10-17
BR8401049A (pt) 1984-10-16
FI86886B (fi) 1992-07-15
NZ207414A (en) 1988-02-29
IE57264B1 (en) 1992-07-01
AR241795A1 (es) 1992-12-30
AU2535784A (en) 1984-09-13
JPS6240999B2 (OSRAM) 1987-09-01
JPS59166086A (ja) 1984-09-19
FI840918L (fi) 1984-09-10
FI840918A0 (fi) 1984-03-07
DK136184A (da) 1984-09-10

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Owner name: BEPPU, TERUHIKO