FI813069A7 - Menetelmä, jolla voidaan stabiloida ja identifioida isäntäsoluja, jotka sisältävät hyödyllisiä polypeptidejä ekspressoivia yhdistelmä-DNA-molekyyleja - Google Patents

Menetelmä, jolla voidaan stabiloida ja identifioida isäntäsoluja, jotka sisältävät hyödyllisiä polypeptidejä ekspressoivia yhdistelmä-DNA-molekyyleja Download PDF

Info

Publication number
FI813069A7
FI813069A7 FI813069A FI813069A FI813069A7 FI 813069 A7 FI813069 A7 FI 813069A7 FI 813069 A FI813069 A FI 813069A FI 813069 A FI813069 A FI 813069A FI 813069 A7 FI813069 A7 FI 813069A7
Authority
FI
Finland
Prior art keywords
stabilizing
host cells
recombinant dna
dna molecules
cells containing
Prior art date
Application number
FI813069A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI813069L (fi
Inventor
Anna Kay Radue
Jr Paul Robert Rosteck
Charles Lee Hershberger
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of FI813069L publication Critical patent/FI813069L/fi
Publication of FI813069A7 publication Critical patent/FI813069A7/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
FI813069A 1980-10-03 1981-10-02 Menetelmä, jolla voidaan stabiloida ja identifioida isäntäsoluja, jotka sisältävät hyödyllisiä polypeptidejä ekspressoivia yhdistelmä-DNA-molekyyleja FI813069A7 (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19355680A 1980-10-03 1980-10-03
US06/275,088 US4506013A (en) 1980-10-03 1981-06-18 Stabilizing and selecting recombinant DNA host cells

Publications (2)

Publication Number Publication Date
FI813069L FI813069L (fi) 1982-04-04
FI813069A7 true FI813069A7 (fi) 1982-04-04

Family

ID=26889117

Family Applications (1)

Application Number Title Priority Date Filing Date
FI813069A FI813069A7 (fi) 1980-10-03 1981-10-02 Menetelmä, jolla voidaan stabiloida ja identifioida isäntäsoluja, jotka sisältävät hyödyllisiä polypeptidejä ekspressoivia yhdistelmä-DNA-molekyyleja

Country Status (21)

Country Link
US (1) US4506013A (enExample)
EP (1) EP0049619B1 (enExample)
JP (2) JPH0636742B2 (enExample)
AU (1) AU543168B2 (enExample)
BR (1) BR8106374A (enExample)
CA (1) CA1189467A (enExample)
DD (1) DD209476A5 (enExample)
DE (1) DE3177100D1 (enExample)
DK (1) DK437281A (enExample)
ES (1) ES506000A0 (enExample)
FI (1) FI813069A7 (enExample)
GB (1) GB2084584B (enExample)
GR (1) GR75339B (enExample)
IE (1) IE51796B1 (enExample)
IL (1) IL63981A (enExample)
NZ (1) NZ198539A (enExample)
PH (1) PH19901A (enExample)
PL (1) PL233296A1 (enExample)
PT (1) PT73768B (enExample)
RO (1) RO87260A (enExample)
SU (1) SU1187725A3 (enExample)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5878589A (ja) * 1981-11-06 1983-05-12 Kanegafuchi Chem Ind Co Ltd プラスミド含有微生物の培養法
US4650761A (en) * 1981-11-27 1987-03-17 Eli Lilly And Company Method for stabilizing and selecting recombinant DNA containing host cell
US4436815A (en) * 1981-11-27 1984-03-13 Eli Lilly And Company Method for stabilizing and selecting recombinant DNA containing host cells
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US5112755A (en) * 1982-04-15 1992-05-12 Genentech, Inc. Preparation of functional human urokinase proteins
IL68366A (en) * 1982-04-15 1990-08-31 Genentech Inc Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture
US4863855A (en) * 1982-05-14 1989-09-05 The Research Foundation Of State University Of New York Novel cloning vehicles for polypeptide expression in microbial hosts
US4530904A (en) * 1982-09-03 1985-07-23 Eli Lilly And Company Method for conferring bacteriophage resistance to bacteria
US4673641A (en) * 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
GB8308483D0 (en) * 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
US4912046A (en) * 1983-06-27 1990-03-27 Genentech, Inc. Portable inducible control system
US4637980A (en) * 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
EP0155189A3 (en) * 1984-03-16 1987-09-16 Genentech, Inc. Expression of cell wall degrading proteins and host cells harboring dna encoding such protein
IL71555A (en) * 1984-04-15 1992-06-21 State Of Israel Israel Inst Fo Bovine interferon
US5723292A (en) * 1984-05-25 1998-03-03 Zymogenetics, Inc. Stable DNA constructs
AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs
US5871957A (en) * 1984-05-25 1999-02-16 Zymogenetics, Inc. Stable DNA constructs
US5143836A (en) * 1984-08-27 1992-09-01 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda pl promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
US5081020A (en) * 1984-08-27 1992-01-14 Bio-Technology General Corp. Expression vectors containing λPL promoter, T1 T2 rRNA termination sequence and origin of replication derived from a constitutive high copy number plasmid hosts and related methods
US5759816A (en) * 1984-08-27 1998-06-02 Bio-Technology General Corp. Expression vectors containing λPL promoter and T1 T2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods
CA1340597C (en) * 1984-08-27 1999-06-22 Haim Aviv Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods
US5162217A (en) * 1984-08-27 1992-11-10 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
US5112744A (en) * 1984-08-27 1992-05-12 Bio-Technology General Corp. Plasmids for the production of human Cu-Zn superoxide dismutase, bacterial hosts containing the plasmids and methods for production and recovery of such superoxide dismutase
US5059529A (en) * 1984-08-27 1991-10-22 Bio-Technology General Corp. Stabilized expression vectors containing λPL promoter and the gene for the cI434 repressor, plasmids containing the vectors, hosts containing the plasmids and related methods
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US4659805A (en) * 1984-12-11 1987-04-21 California Biotechnology, Inc. Recombinant alveolar surfactant protein
US4912038A (en) * 1984-12-11 1990-03-27 California Biotechnology Inc. Recombinant DNA sequence encoding alveolar surfactant protein
US5840527A (en) * 1984-12-11 1998-11-24 Byk Gulden Lomberg Chemische Fabrik Gmbh Recombinant alveolar surfactant protein
US5169761A (en) * 1984-12-11 1992-12-08 California Biotechnology Inc. Dna encoding and expression systems for alveolar surfactant proteins
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
US5028530A (en) * 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
US5206154A (en) * 1985-01-28 1993-04-27 Xoma Corporation Method of producing cecropins by microbiological techniques
DK275685D0 (da) * 1985-06-18 1985-06-18 Benzon As Alfred Stabilisering af biologiske systemer
SE8505027D0 (sv) * 1985-10-24 1985-10-24 Kabigen Ab A lytic virulent phage, a hostcell containing same and a process for protein production
DE3750125T2 (de) * 1986-03-26 1994-10-27 Genexpress Aps Biologische eindämmung.
US5279939A (en) * 1986-08-18 1994-01-18 Smithkline Beecham Corporation Protein protease inhibitors from streptomyces
WO1988005820A1 (en) * 1987-01-29 1988-08-11 California Biotechnology, Inc. Recombinant alveolar surfactant protein
NL8701450A (nl) * 1987-06-22 1989-01-16 Solvay Werkwijze voor het transformeren van cellen.
US4960707A (en) * 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
US5002875A (en) * 1987-08-28 1991-03-26 The United States Of America As Represented By The United States Department Of Energy Plasimids containing the gene for DNA polymerase I from Streptococcus pneumoniae
US5096815A (en) * 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
US5198346A (en) * 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
JPH11507532A (ja) * 1995-06-07 1999-07-06 ワシントン ユニバーシティ 環境に制限される生存能を有する組換え細菌システム
JP5918125B2 (ja) * 2009-05-22 2016-05-18 メリアル リミテッド 抗生物質フリープラスミド

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MW3378A1 (en) * 1977-11-08 1980-03-12 Genentech Inc Method and means for microbial polypeptide expression;synthetic dna and process therefor
US4366246A (en) * 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
US4356270A (en) * 1977-11-08 1982-10-26 Genentech, Inc. Recombinant DNA cloning vehicle
EP0009930B2 (en) * 1978-10-10 1988-08-24 The Board Of Trustees Of The Leland Stanford Junior University Recombinant dna, method for preparing it and production of foreign proteins by unicellular hosts containing it
JPS55156591A (en) * 1979-05-23 1980-12-05 Ajinomoto Co Inc Method for stabilizing property of bacteria containing plasmid

Also Published As

Publication number Publication date
FI813069L (fi) 1982-04-04
GB2084584B (en) 1984-02-08
ES8305413A1 (es) 1982-12-16
JPH05292951A (ja) 1993-11-09
IE812297L (en) 1982-04-03
ES506000A0 (es) 1982-12-16
CA1189467A (en) 1985-06-25
IL63981A0 (en) 1982-01-31
RO87260A (ro) 1986-07-30
JPS57132885A (en) 1982-08-17
EP0049619B1 (en) 1989-09-06
AU7601281A (en) 1982-04-08
AU543168B2 (en) 1985-04-04
DE3177100D1 (en) 1989-10-12
DD209476A5 (de) 1984-05-09
SU1187725A3 (ru) 1985-10-23
DK437281A (da) 1982-04-04
JPH0669369B2 (ja) 1994-09-07
BR8106374A (pt) 1982-06-22
PH19901A (en) 1986-08-13
IE51796B1 (en) 1987-04-01
PT73768A (en) 1981-11-01
GB2084584A (en) 1982-04-15
NZ198539A (en) 1985-07-12
PL233296A1 (en) 1983-07-18
EP0049619A3 (en) 1983-03-16
PT73768B (en) 1983-11-30
US4506013A (en) 1985-03-19
EP0049619A2 (en) 1982-04-14
GR75339B (enExample) 1984-07-13
JPH0636742B2 (ja) 1994-05-18
IL63981A (en) 1989-01-31

Similar Documents

Publication Publication Date Title
FI813069A7 (fi) Menetelmä, jolla voidaan stabiloida ja identifioida isäntäsoluja, jotka sisältävät hyödyllisiä polypeptidejä ekspressoivia yhdistelmä-DNA-molekyyleja
DK61595A (da) Rekombinant DNA-molekyler, værtscelletransformering dermed, værtsceller indeholdende disse samt fremgangsmåde til fremstilling af et polypeptid af IFN-alfa-typen
IL67176A0 (en) Method for stabilizing and selecting recombinant dna containing host cells
DK559186D0 (da) Beholder til laese, flydende materialer
DK75986D0 (da) Alfa, omega-dicarboxylsyrer og fremgangsmaade til fremstilling deraf samt laegemidler indeholdende disse forbindelser
YU43634B (en) Process for obtaining recombinant dna comprising an amylase coding gene
FI880899L (fi) Oleellisesti puhdas DNA ja RNA ja menetelmä erytropoietiinin ekspressoimiseksi
NL940022I1 (nl) Recombinant-dna-moleculen
DE3672059D1 (de) Verfahren zum komprimieren von zwei-pegeldaten.
NO830560L (no) Belagt elektrode for elektrolyse og fremgangsmaate ved fremstilling derav.
AU3038789A (en) Dry ion-selective electrodes for the determination of ionic species in aqueous media
NO174970C (no) Rekombinant DNA, rekombinant DNA-vektor samt vertscelle
NO871648L (no) Fremgangsmaate ved raffinering av fibermateriale.
NO860550L (no) Polypeptider av rhinovirus, samt dna-molekyler som koder for slike.
DE3671850D1 (de) Vorrichtung zum handhaben von gusspfannen.
DE3483618D1 (de) Analytisches element zur mengenbestimmung von bilirubin.
IT8009398A0 (it) Apparecchio per la lettura ottico-densitometrica di campioni, in specie elettroforetici, con supporto discoidale porta-campioni
DE3689095D1 (de) Dielektrischer wellenleiter zur verwendung in einem analyseverfahren.
DK439883A (da) Referenceelektrodesystem til et potentiometrisk analyseinstrument
DK190183D0 (da) Modificerede lerarter samt fremgangsmade til fremstilling deraf
IT7949424A0 (it) Reagente di controllo di riferimento di analisi ematologica multipla e metodo per la sua realizzazione
DE3475005D1 (en) Ion sensing electrode and methods for producing such electrodes and ion-selective membranes therefor
NO852447L (no) Fremgangsmaate til reduksjon av datavolumet ved seismisk databehandling.
DK440387D0 (da) Fremgangsmaade til udvaelgelse af streptomyces-vaertsceller og andre vaertsceller indeholdende rekombinant dna
NO850732L (no) Rekombinante dna-molekyler

Legal Events

Date Code Title Description
FD Application lapsed

Owner name: ELI LILLY AND COMPANY