FI80801C - Konjugat av en monoklonal antikropp eller ett fragment därav och ett k elaterande ämne, dess framställning och användning - Google Patents

Konjugat av en monoklonal antikropp eller ett fragment därav och ett k elaterande ämne, dess framställning och användning Download PDF

Info

Publication number
FI80801C
FI80801C FI870122A FI870122A FI80801C FI 80801 C FI80801 C FI 80801C FI 870122 A FI870122 A FI 870122A FI 870122 A FI870122 A FI 870122A FI 80801 C FI80801 C FI 80801C
Authority
FI
Finland
Prior art keywords
ett
fragment
och
conjugate
antibody
Prior art date
Application number
FI870122A
Other languages
English (en)
Finnish (fi)
Other versions
FI870122A0 (fi
FI80801B (fi
FI870122A (fi
Inventor
Pirkko Vihko
Marja Soedervall
Reijo Vihko
Original Assignee
Innofarm Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innofarm Oy filed Critical Innofarm Oy
Priority to FI870122A priority Critical patent/FI80801C/sv
Publication of FI870122A0 publication Critical patent/FI870122A0/fi
Priority to PCT/FI1987/000163 priority patent/WO1988005537A1/en
Publication of FI870122A publication Critical patent/FI870122A/fi
Publication of FI80801B publication Critical patent/FI80801B/fi
Application granted granted Critical
Publication of FI80801C publication Critical patent/FI80801C/sv

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (6)

1. Med ett fluoreserande ämne märkt konjugat av en mono-klonal antikropp eller ett fragment därav, och ett kela-terande ämne för in vitro-diagnostik, känneteck- 12 80801 n a t därav, att det kelaterande ämnet är mono- eller bicyklisk DTPA.
2. Konjugat enligt patentkravet 1, känneteck- n a t därav, att den monoklonala antikroppen eller dess fragment är antikropp för SHBG-antigenen eller dess fragment .
3. Konjugat enligt patentkravet 1, känneteck-n a t därav att den monoklonala antikroppen eller dess fragment är antikropp för PAP-antigenen eller dess fragment .
4. Konjugat enligt patentkraven 1-3, känneteck-n a t därav, att det fluoreserande ämnet är Eu.
5. Förfarande för framställning av ett konjugat enligt patentkraven 1-4, kännetecknat därav, att den biologiska molekylen och det kelaterande ämnet först kopplas ihop varefter konjugatet märks med ett fluoreserande ämne.
6. In vitro-diagnostiskt immunologiskt förfarande, kännetecknat därav, att man använder ett konjugat enligt patentkraven 1-4 för bestämning av en ana-lyts antigen. l!
FI870122A 1987-01-14 1987-01-14 Konjugat av en monoklonal antikropp eller ett fragment därav och ett k elaterande ämne, dess framställning och användning FI80801C (sv)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FI870122A FI80801C (sv) 1987-01-14 1987-01-14 Konjugat av en monoklonal antikropp eller ett fragment därav och ett k elaterande ämne, dess framställning och användning
PCT/FI1987/000163 WO1988005537A1 (en) 1987-01-14 1987-12-08 Conjugate of biological molecules and a chelating agent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI870122A FI80801C (sv) 1987-01-14 1987-01-14 Konjugat av en monoklonal antikropp eller ett fragment därav och ett k elaterande ämne, dess framställning och användning
FI870122 1987-01-14

Publications (4)

Publication Number Publication Date
FI870122A0 FI870122A0 (fi) 1987-01-14
FI870122A FI870122A (fi) 1988-07-15
FI80801B FI80801B (fi) 1990-03-30
FI80801C true FI80801C (sv) 1990-07-10

Family

ID=8523765

Family Applications (1)

Application Number Title Priority Date Filing Date
FI870122A FI80801C (sv) 1987-01-14 1987-01-14 Konjugat av en monoklonal antikropp eller ett fragment därav och ett k elaterande ämne, dess framställning och användning

Country Status (2)

Country Link
FI (1) FI80801C (sv)
WO (1) WO1988005537A1 (sv)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5792444A (en) * 1989-05-09 1998-08-11 The General Hospital Corporation Labeled chemotactic peptides to image focal sites of infection or inflammation
EP0709100A1 (en) * 1994-09-30 1996-05-01 Nihon Medi-Physics Co., Ltd. Contrast agent, containing several detection groups per molecule

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4647447A (en) * 1981-07-24 1987-03-03 Schering Aktiengesellschaft Diagnostic media
US4668503A (en) * 1982-07-26 1987-05-26 Trustees Of University Of Massachusetts Process for labeling amines with 99m Tc
US4479930A (en) * 1982-07-26 1984-10-30 Trustees Of The University Of Massachusetts Amines coupled wth dicyclic dianhydrides capable of being radiolabeled product
NL194579C (nl) * 1983-01-21 2002-08-05 Schering Ag Diagnostisch middel.
SE465907B (sv) * 1984-11-01 1991-11-18 Nyegaard & Co As Diagnosticeringsmedel innehaallande en paramagnetisk metall
DD229595A1 (de) * 1984-12-14 1985-11-13 Adw Ddr Verfahren zur herstellung von mit chelatbildnern gekoppelten biomolekuelen
WO1987007955A1 (en) * 1986-06-17 1987-12-30 Baxter Travenol Laboratories, Inc. Homogeneous fluoroassay methods employing fluorescent background rejection and water-soluble rare earth metal chelate fluorophores

Also Published As

Publication number Publication date
FI870122A0 (fi) 1987-01-14
WO1988005537A1 (en) 1988-07-28
FI80801B (fi) 1990-03-30
FI870122A (fi) 1988-07-15

Similar Documents

Publication Publication Date Title
US4808541A (en) Determination method utilizing reagents covalently labelled with essentially non-fluorescent lanthanide chelates in combination with time-resolved fluorescence spectroscopy and the reagents to be used in the method
US4476229A (en) Substituted carboxyfluoresceins
JP2866419B2 (ja) 希土類クリプテート及びその製造方法とその合成中間体とその蛍光トレーサーとしての利用方法
Mukkala et al. The synthesis and use of activated N-benzyl derivatives of diethylenetriaminetetraacetic acids: alternative reagents for labeling of antibodies with metal ions
Bailey et al. Terbium chelate for use as a label in fluorescent immunoassays
RU2111494C1 (ru) Способ определения гликолизированного гемоглобина в присутствии негликолизированного гемоглобина, аналитический тестовый набор
US4082738A (en) Cyanocobalamin derivatives
JPS58757A (ja) 同位体を使用しない免疫検定を実施する方法、ラベルされた分析物とかかる検定に使用するキット
EP0493745A1 (en) Fluorescent compound, complex, reagent, and specific binding assay employing said reagent
US4337339A (en) Process for preparation of folic acid derivatives
JPH02504640A (ja) 表面カップリング官能性を有するイットリウム、ランタニドおよびアクチニドの大環状錯体
JPH0137693B2 (sv)
CA1152490A (en) Beta-galactosyl-umbelliferone-labeled protein and polypeptide conjugates
US20210395606A1 (en) Chromophoric structures for macrocyclic lanthanide chelates
FI87022C (sv) Märkta och märkningsbara reagenser för fluorometriska bestämningar
JPH08208691A (ja) オリゴペプチド接合体
CN101865917A (zh) 三碘甲腺原氨酸的检测试剂盒及其使用方法
JP3713087B2 (ja) ハプテンのイムノアッセイおよびそれに使用可能なハプテントレーサー抗体複合体ならびにそれらの製造方法
Yang et al. Time-resolved fluorescence immunoassay with measurement of a europium chelate in solution: dissociation conditions and application for determination of cortisol
EP0100543B1 (en) Method for quantitative analysis of low molecular weight components contained in protein-containing liquid sample
FI80801C (sv) Konjugat av en monoklonal antikropp eller ett fragment därav och ett k elaterande ämne, dess framställning och användning
US3983099A (en) Thyroxine-and triiodothyronine-tyrosine dipeptide derivatives
US6190923B1 (en) Diethylenetriamine-N,N′,N″-triacetic acid derivatives
SAWAI et al. Sensitive Radioimmuno Assay for 2′, 5′-Oligoadenylates Using a Novel 125I-Labeled Derivative of 2′, 5′-TriadenyIate 5′-Triphosphate
CN102539761A (zh) 一种促甲状腺素定量检测试剂盒(时间分辨荧光免疫法)制备方法

Legal Events

Date Code Title Description
MM Patent lapsed

Owner name: INNOFARM OY