FI79552B - Plasmidvektorer och foerfarande foer framstaellning daerav. - Google Patents
Plasmidvektorer och foerfarande foer framstaellning daerav. Download PDFInfo
- Publication number
- FI79552B FI79552B FI843600A FI843600A FI79552B FI 79552 B FI79552 B FI 79552B FI 843600 A FI843600 A FI 843600A FI 843600 A FI843600 A FI 843600A FI 79552 B FI79552 B FI 79552B
- Authority
- FI
- Finland
- Prior art keywords
- plasmid
- dna
- pbr322
- gene
- plasmids
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (6)
1. Plasmidvektorer som producerar ett stort antal kopior, kännetecknade av att de har samma replikations-omräde som pBR322 och innehäller i det genomräde som sva-rar för produktionen av repressorn för plasmidens replikation en mutation vid transkriptorterminalen för modifiering av repressorproduktionen och a) innehäller säsom selektiv genmarkör plasmidens pBR322 ampicillinresistensgen och i nämnda plasmidvektorer Sr tet-racyklinresistensgenen inaktiverad eller saknas helt eller har en represserad funktion, eller b) innehäller säsom selektiva genmarkörer plasmidens pBR322 ampicillinresistensgen och tetracyklinresistensgen, men i nämnda plasmidvektorer utförs tetracyklinresistensge-nens transkription frän en mutant som är svagare än den naturliga mutanten eller frän en främmande promotor.
2. Plasmidvektorer enligt patentkravet 1, kännetecknade av att de har en G->T punktmutation i den gen som kodar för det lilla RNA som fungerar som repressor för replikationen.
3. Plasmidvektorer, kännetecknade av att de är pHC 314 (00280,OKI, Ungern), pHC 312 (00279, OKI, Ungern) och pHC 624 (00281, OKI, Ungern).
4. Förfarande för framställning av ett stort antal kopior producerande plasmidvektorer frän sädana plasmider som upp-visar samma replikationsomräde som pBR322, k ä n n e -tecknat av att i det genomräde som svarar för produktionen av repressorn för plasmidreplikationen ästadkommes i transkriptorterminalen en mutation som ändrar repressorproduktionen.
5. Förfarande enligt patentkrav 4, kännetecknat av att i pBR322, vars tetracyklinresistensgen är i inaktive-
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU321283 | 1983-09-16 | ||
| HU833212A HU194307B (en) | 1983-09-16 | 1983-09-16 | Process for producing plasmidvectors of high copy number |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI843600A0 FI843600A0 (fi) | 1984-09-14 |
| FI843600L FI843600L (fi) | 1985-03-17 |
| FI79552B true FI79552B (fi) | 1989-09-29 |
| FI79552C FI79552C (sv) | 1990-01-10 |
Family
ID=10963133
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI843600A FI79552C (sv) | 1983-09-16 | 1984-09-14 | Plasmidvektorer och förfarande för framställning därav. |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US4703012A (sv) |
| JP (1) | JPS60156389A (sv) |
| AT (1) | AT384031B (sv) |
| AU (1) | AU578041B2 (sv) |
| BE (1) | BE900570A (sv) |
| CA (1) | CA1275631C (sv) |
| CH (1) | CH667672A5 (sv) |
| DE (1) | DE3434041A1 (sv) |
| DK (1) | DK439784A (sv) |
| FI (1) | FI79552C (sv) |
| FR (1) | FR2552102B1 (sv) |
| GB (1) | GB2148301B (sv) |
| HU (1) | HU194307B (sv) |
| IL (1) | IL72952A (sv) |
| IT (1) | IT1178510B (sv) |
| NL (1) | NL8402846A (sv) |
| PL (1) | PL152527B1 (sv) |
| SE (1) | SE463266B (sv) |
| SU (1) | SU1443806A3 (sv) |
| ZA (1) | ZA847273B (sv) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE67243T1 (de) * | 1985-10-21 | 1991-09-15 | Fujisawa Pharmaceutical Co | Verfahren zur herstellung von insulinaehnlichem wachstumsfaktor i (igf-i) und plasmid zu dessen herstellung. |
| DE4136389A1 (de) * | 1991-03-18 | 1992-10-08 | Hoechst Ag | Verfahren zur herstellung von glutarylacylase in grossen mengen |
| JPH06327480A (ja) * | 1993-05-20 | 1994-11-29 | Mitsubishi Petrochem Co Ltd | プラスミドの自律複製を司る機能に関与する遺伝子を含むｄｎａ断片 |
| US20040259252A1 (en) * | 2001-07-18 | 2004-12-23 | Sanders Mitchell C. | Vector containing an enhanced p15a origin of replication |
| US8999672B2 (en) | 2005-01-28 | 2015-04-07 | James A. Williams | Compositions and processes for improved plasmid DNA production |
| DE602006013479D1 (de) * | 2005-09-16 | 2010-05-20 | Monsanto Technology Llc | Plasmide mit mobilen hybridreplikationsursprüngen |
| BRPI0721630A2 (pt) | 2007-05-02 | 2013-10-15 | Merial Ltd | Plasmídeo de dna tendo expressão e estabilidade aperfeiçoadas |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2759053A1 (de) * | 1977-12-30 | 1979-07-12 | Uhlin | Verfahren zum herstellen von genprodukten von plasmid-dns |
| FI793884A7 (sv) * | 1978-12-26 | 1981-01-01 | Cetus Corp | Stabila plasmider med stort kopieantal. |
| CA1198067A (en) * | 1981-02-27 | 1985-12-17 | David H. Gelfand | Stable high copy number plasmids |
| CA1209501A (en) * | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
| GB8308236D0 (en) * | 1983-03-25 | 1983-05-05 | Celltech Ltd | Dna vectors |
| EP0126166B1 (en) * | 1983-05-11 | 1990-01-31 | Fina Research S.A. | Cloning vectors comprising a restriction site bank and the construction thereof |
-
1983
- 1983-09-16 HU HU833212A patent/HU194307B/hu not_active IP Right Cessation
-
1984
- 1984-09-12 CH CH4367/84A patent/CH667672A5/de not_active IP Right Cessation
- 1984-09-13 BE BE1/011088A patent/BE900570A/fr not_active IP Right Cessation
- 1984-09-13 GB GB08423161A patent/GB2148301B/en not_active Expired
- 1984-09-14 FR FR8414122A patent/FR2552102B1/fr not_active Expired
- 1984-09-14 CA CA000463157A patent/CA1275631C/en not_active Expired - Lifetime
- 1984-09-14 AT AT0293584A patent/AT384031B/de not_active IP Right Cessation
- 1984-09-14 ZA ZA847273A patent/ZA847273B/xx unknown
- 1984-09-14 SU SU843792252A patent/SU1443806A3/ru active
- 1984-09-14 SE SE8404637A patent/SE463266B/sv not_active Application Discontinuation
- 1984-09-14 PL PL1984249593A patent/PL152527B1/pl unknown
- 1984-09-14 IL IL72952A patent/IL72952A/xx unknown
- 1984-09-14 FI FI843600A patent/FI79552C/sv not_active IP Right Cessation
- 1984-09-14 AU AU33077/84A patent/AU578041B2/en not_active Ceased
- 1984-09-14 NL NL8402846A patent/NL8402846A/nl not_active Application Discontinuation
- 1984-09-14 IT IT22677/84A patent/IT1178510B/it active
- 1984-09-14 JP JP59191860A patent/JPS60156389A/ja active Pending
- 1984-09-14 DK DK439784A patent/DK439784A/da not_active Application Discontinuation
- 1984-09-17 DE DE19843434041 patent/DE3434041A1/de not_active Withdrawn
- 1984-09-17 US US06/651,679 patent/US4703012A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| PL249593A1 (en) | 1985-07-16 |
| SE8404637D0 (sv) | 1984-09-14 |
| ZA847273B (en) | 1986-01-29 |
| FI79552C (sv) | 1990-01-10 |
| FI843600L (fi) | 1985-03-17 |
| ATA293584A (de) | 1987-02-15 |
| DK439784D0 (da) | 1984-09-14 |
| IL72952A (en) | 1990-11-05 |
| GB8423161D0 (en) | 1984-10-17 |
| NL8402846A (nl) | 1985-04-16 |
| FR2552102B1 (fr) | 1987-09-18 |
| JPS60156389A (ja) | 1985-08-16 |
| SE463266B (sv) | 1990-10-29 |
| GB2148301B (en) | 1988-05-11 |
| AU3307784A (en) | 1985-03-21 |
| HU194307B (en) | 1988-01-28 |
| IT8422677A0 (it) | 1984-09-14 |
| DK439784A (da) | 1985-03-17 |
| US4703012A (en) | 1987-10-27 |
| CH667672A5 (de) | 1988-10-31 |
| FI843600A0 (fi) | 1984-09-14 |
| DE3434041A1 (de) | 1985-09-12 |
| CA1275631C (en) | 1990-10-30 |
| PL152527B1 (en) | 1991-01-31 |
| SE8404637L (sv) | 1985-03-17 |
| SU1443806A3 (ru) | 1988-12-07 |
| GB2148301A (en) | 1985-05-30 |
| IT1178510B (it) | 1987-09-09 |
| AT384031B (de) | 1987-09-25 |
| HUT35280A (en) | 1985-06-28 |
| IT8422677A1 (it) | 1986-03-14 |
| FR2552102A1 (fr) | 1985-03-22 |
| AU578041B2 (en) | 1988-10-13 |
| BE900570A (fr) | 1985-03-13 |
| IL72952A0 (en) | 1984-12-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM | Patent lapsed | ||
| MM | Patent lapsed |
Owner name: RICHTER GEDEON VEGYESZETI GYAR R.T. |