FI60888C - Foerfarande foer upptaeckande av patogena mikroorganismer i ett vaetskeformigt prov och vid foerfarandet anvaendbar anordning - Google Patents
Foerfarande foer upptaeckande av patogena mikroorganismer i ett vaetskeformigt prov och vid foerfarandet anvaendbar anordning Download PDFInfo
- Publication number
- FI60888C FI60888C FI773237A FI773237A FI60888C FI 60888 C FI60888 C FI 60888C FI 773237 A FI773237 A FI 773237A FI 773237 A FI773237 A FI 773237A FI 60888 C FI60888 C FI 60888C
- Authority
- FI
- Finland
- Prior art keywords
- liquid
- layer
- sample
- pathogenic microorganisms
- centrifuge
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Centrifugal Separators (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Claims (8)
1. Förfarande för upptackande av i ett vätskeformigt prov möjligen ingäende patogena mikroorganismer genom att placera provet i en sluten, steril centrifugeringszon och genom att centrifugera, sä att det nämnda provet trycks mot det vätskeformiga mediets yta, och genom att härefter separera det nämnda vätskeformiga mediet och de däri ingäende patogena mikroorganismerna frän nämnda centrifugeringszon, och genom att analy-sera detta för upptackande av nämnda patogena mikroorganismer, k ä n -netecknat därav, att a) det nämnda provet, säsom blod, benmärgsvätska, ryggmärgsvätska, lungsäcksvätska eller urin, genom centrifugering vid 1500-3000 x g pressas mot en ett skickt bildande vätska, vilken är ogiftig för de nämnda patogena mikroorganismerna, hydrofob och icke blandbar med vatten, och vars täthet är större än det nämnda provets täthet, säsom ett inert fluorerat kolväte med tätheten ca. 1,2-2,0 g/cm^, kokpunkten ca. 90-210°C och en genomsnittlig molekylvikt av ca. 300-850, varvid centrifugalkraften fär verka sä länge, att de patogena mikroorganismerna hinner förflyttas ända fram tili den nämnda ett skikt bildande vätskan, men de hinner inte förflyttas genom detta vätskeskikt, sä att huduvddelen av de närvarande patogena mikroorganismerna koncentreras i den ett skikt bildande vätskan och i gränsskiktet mellan denna och det vätskeformiga provet, b) efter centrifugeringen avlägsnas huvuddelen av det vätskeformiga provet utan att dess understa, pä den ett skikt bildande vätskan liggande del vidrörs, sä att de i gränsskiktet eller närä detta befintliga patogena mikroorganismerna inte avlägsnas, och c) den resterande, den ett skikt bildande vätskan och det vätskeformiga provets understa del samt de patogena mikroorganismerna omfattande ämnes-blandningen analyseras.
2. Förfarande enligt patentkravet 1, kännetecknat därav, att den nämnda ett skikt bildande vätskan är närvarande i en mängd av 3,3-30,0 volym-%.
3. Förfarande enligt patentkravet 1, kännetecknat därav, att den ett skikt bildande vätskan utgörs av ett inert, perfluorerat kolväte med en täthet av ca. 1,7-2,0 g/cm , en kokpunkt av ca. 125-200 C och en genomsnittlig molekylvikt av ca. 350-600.
4. Anordning användbar vid förfarandet enligt patentkravet 1, k ä n -netecknad därav, att den omfattar ett slutet centrifugalkärl (20),
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/739,274 US4131512A (en) | 1976-11-05 | 1976-11-05 | Method for detecting microbial pathogens employing a cushioning agent |
| US73927476 | 1976-11-05 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI773237A FI773237A (fi) | 1978-05-06 |
| FI60888B FI60888B (fi) | 1981-12-31 |
| FI60888C true FI60888C (fi) | 1982-04-13 |
Family
ID=24971572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI773237A FI60888C (fi) | 1976-11-05 | 1977-10-28 | Foerfarande foer upptaeckande av patogena mikroorganismer i ett vaetskeformigt prov och vid foerfarandet anvaendbar anordning |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US4131512A (sv) |
| JP (1) | JPS5386012A (sv) |
| AT (1) | AT359207B (sv) |
| AU (1) | AU519142B2 (sv) |
| BE (1) | BE860490A (sv) |
| CA (1) | CA1098429A (sv) |
| CH (1) | CH636128A5 (sv) |
| DE (1) | DE2747496A1 (sv) |
| DK (1) | DK153763C (sv) |
| ES (1) | ES463854A1 (sv) |
| FI (1) | FI60888C (sv) |
| FR (1) | FR2393063A1 (sv) |
| GB (1) | GB1546043A (sv) |
| IL (1) | IL53258A (sv) |
| IT (1) | IT1090428B (sv) |
| NL (1) | NL180253C (sv) |
| NO (1) | NO150521C (sv) |
| NZ (1) | NZ185557A (sv) |
| SE (1) | SE447485B (sv) |
| ZA (1) | ZA776438B (sv) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4212948A (en) * | 1978-10-18 | 1980-07-15 | J. K. And Susie L. Wadley Research Institute And Blood Bank | Apparatus for detecting microbial pathogens employing a cushioning agent |
| US4342724A (en) * | 1980-08-21 | 1982-08-03 | E. R. Squibb & Sons, Inc. | Red cell labeling vial |
| US5070014A (en) * | 1982-09-30 | 1991-12-03 | Wadley Technologies, Inc. | Stabilization of specimens for microbial analysis |
| US4581331A (en) * | 1983-09-29 | 1986-04-08 | E. I. Du Pont De Nemours And Company | Method for the rapid detection of virus and viral antigens |
| US4693972A (en) * | 1984-01-16 | 1987-09-15 | Becton, Dickinson And Company | Composition and method for rapid detection of microorganisms in clinical samples |
| US5043267A (en) * | 1984-05-18 | 1991-08-27 | E. I. Du Pont De Nemours And Company | Method for rapid detection of bacterial and fungal infection |
| WO1995015397A1 (en) * | 1993-12-03 | 1995-06-08 | Dorn Gordon L | Method for improving quantitative recovery of microorganisms fro m specimens containing blood components |
| FR2732037B1 (fr) * | 1995-03-20 | 1997-05-30 | Dbv | Procede de detection de microorganismes par separation et culture sur un systeme gelifie, systeme gelifie et necessaire de dosage pour mettre en oeuvre ce procede, utilisation en microbiologie |
| DE19801090A1 (de) * | 1998-01-14 | 1999-07-15 | Gerhard Haase | Lysis-Filtrations-Assay LYFA |
| CN102271814B (zh) * | 2008-10-31 | 2015-07-08 | 生物梅里埃公司 | 用于微生物的分离、表征和/或鉴定的分离装置 |
| WO2010062350A1 (en) * | 2008-10-31 | 2010-06-03 | Biomerieux, Inc. | Methods for detection, characterization and/or identification of microorganisms in a sealed container |
| US8647835B2 (en) | 2008-10-31 | 2014-02-11 | BIO MéRIEUX, INC. | Methods for separation, characterization and/or identification of microorganisms using spectroscopy |
| US10059975B2 (en) * | 2008-10-31 | 2018-08-28 | Biomerieux, Inc. | Methods for the isolation and identification of microorganisms |
| WO2010062354A1 (en) * | 2008-10-31 | 2010-06-03 | Biomerieux, Inc. | Methods for separation, characterization, and/or identification of microorganisms using mass spectrometry |
| JP2012507710A (ja) * | 2008-10-31 | 2012-03-29 | バイオメリュー・インコーポレイテッド | 同定作用物質を使用した微生物の分離、キャラクタリゼーションおよび／または同定方法 |
| AU2009320337B2 (en) * | 2008-10-31 | 2014-07-31 | Biomerieux, Inc. | Methods for separation, characterization and/or identification of microorganisms using spectroscopy |
| KR20110095277A (ko) * | 2008-10-31 | 2011-08-24 | 바이오메리욱스, 인코포레이티드. | 라만 분광법을 이용하여 미생물을 분리, 특성규명, 및/또는 동정하는 방법 |
| US9707555B2 (en) | 2012-11-30 | 2017-07-18 | Qvella Corporation | Method for pretreatment of microbial samples |
| US8975060B2 (en) * | 2012-11-30 | 2015-03-10 | Qvella Corporation | Method for pretreatment of microbial samples |
| EP3142796A4 (en) | 2014-05-16 | 2017-12-20 | Qvella Corporation | Apparatus, system and method for performing automated centrifugal separation |
| US10870110B2 (en) * | 2015-12-11 | 2020-12-22 | Babson Diagnostics, Inc. | Specimen container and centrifugation method for separating serum or plasma from whole blood therewith |
| WO2019023218A1 (en) * | 2017-07-24 | 2019-01-31 | Epitope Diagnostics, Inc. | DEVICE AND METHOD FOR COLLECTING FECAL SAMPLE AND EXTRACTING ANALYTE |
| US12050052B1 (en) | 2021-08-06 | 2024-07-30 | Babson Diagnostics, Inc. | Refrigerated carrier device for biological samples |
| US12025629B2 (en) | 2022-04-06 | 2024-07-02 | Babson Diagnostics, Inc. | Automated centrifuge loader |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE617137A (sv) * | 1961-05-02 | |||
| US3519400A (en) * | 1967-01-25 | 1970-07-07 | Atomic Energy Commission | Method of centrifugal separation and recovery of chemical species utilizing a liquid medium |
| US3928139A (en) * | 1973-02-12 | 1975-12-23 | Wadley Res Inst & Blood Bank | Detection of microbial pathogens |
| US3875012A (en) * | 1974-01-30 | 1975-04-01 | Wadley Res Inst & Blood Bank | Apparatus and method for the detection of microbial pathogens |
| US3932222A (en) * | 1974-12-20 | 1976-01-13 | J. K. & Susie L. Wadley Research Institute And Blood Bank | For isolating pathogenic microorganisms |
-
1976
- 1976-11-05 US US05/739,274 patent/US4131512A/en not_active Expired - Lifetime
-
1977
- 1977-10-22 DE DE19772747496 patent/DE2747496A1/de active Granted
- 1977-10-24 AU AU29995/77A patent/AU519142B2/en not_active Expired
- 1977-10-28 FI FI773237A patent/FI60888C/fi not_active IP Right Cessation
- 1977-10-28 CH CH1315177A patent/CH636128A5/de not_active IP Right Cessation
- 1977-10-28 ZA ZA00776438A patent/ZA776438B/xx unknown
- 1977-10-31 NZ NZ185557A patent/NZ185557A/xx unknown
- 1977-10-31 IL IL53258A patent/IL53258A/xx unknown
- 1977-11-01 NL NLAANVRAGE7712022,A patent/NL180253C/xx not_active IP Right Cessation
- 1977-11-02 FR FR7732920A patent/FR2393063A1/fr active Granted
- 1977-11-04 DK DK492777A patent/DK153763C/da not_active IP Right Cessation
- 1977-11-04 IT IT51693/77A patent/IT1090428B/it active
- 1977-11-04 NO NO773796A patent/NO150521C/no unknown
- 1977-11-04 ES ES463854A patent/ES463854A1/es not_active Expired
- 1977-11-04 GB GB45958/77A patent/GB1546043A/en not_active Expired
- 1977-11-04 AT AT789477A patent/AT359207B/de not_active IP Right Cessation
- 1977-11-04 BE BE182350A patent/BE860490A/xx not_active IP Right Cessation
- 1977-11-04 CA CA290,205A patent/CA1098429A/en not_active Expired
- 1977-11-04 SE SE7712508A patent/SE447485B/sv not_active IP Right Cessation
- 1977-11-05 JP JP13297077A patent/JPS5386012A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DE2747496A1 (de) | 1978-05-18 |
| FR2393063A1 (fr) | 1978-12-29 |
| IL53258A0 (en) | 1977-12-30 |
| FI773237A (fi) | 1978-05-06 |
| NO150521B (no) | 1984-07-23 |
| AT359207B (de) | 1980-10-27 |
| SE447485B (sv) | 1986-11-17 |
| ES463854A1 (es) | 1978-06-16 |
| DK153763B (da) | 1988-08-29 |
| SE7712508L (sv) | 1978-05-06 |
| NL180253C (nl) | 1987-01-16 |
| JPS5733040B2 (sv) | 1982-07-14 |
| NO773796L (no) | 1978-05-08 |
| DK492777A (da) | 1978-05-06 |
| NO150521C (no) | 1984-10-31 |
| FR2393063B1 (sv) | 1981-11-27 |
| AU519142B2 (en) | 1981-11-12 |
| JPS5386012A (en) | 1978-07-29 |
| IT1090428B (it) | 1985-06-26 |
| GB1546043A (en) | 1979-05-16 |
| DK153763C (da) | 1989-09-04 |
| IL53258A (en) | 1981-05-20 |
| CA1098429A (en) | 1981-03-31 |
| NL7712022A (nl) | 1978-05-09 |
| AU2999577A (en) | 1979-05-03 |
| NL180253B (nl) | 1986-08-18 |
| CH636128A5 (de) | 1983-05-13 |
| ATA789477A (de) | 1980-03-15 |
| US4131512A (en) | 1978-12-26 |
| ZA776438B (en) | 1978-06-28 |
| BE860490A (fr) | 1978-05-05 |
| DE2747496C2 (sv) | 1988-03-17 |
| NZ185557A (en) | 1980-11-14 |
| FI60888B (fi) | 1981-12-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MA | Patent expired | ||
| MA | Patent expired |
Owner name: J.K. AND SUSIE L. WADLEY RESEARCH |