FI120149B - Renat P5 protein av icke-typbar Haemophilus influenzae som vaccin mot en icke-typbar Haemophilus influenzae -stam - Google Patents
Renat P5 protein av icke-typbar Haemophilus influenzae som vaccin mot en icke-typbar Haemophilus influenzae -stam Download PDFInfo
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- FI120149B FI120149B FI952144A FI952144A FI120149B FI 120149 B FI120149 B FI 120149B FI 952144 A FI952144 A FI 952144A FI 952144 A FI952144 A FI 952144A FI 120149 B FI120149 B FI 120149B
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- protein
- haemophilus influenzae
- bacterium
- exchange column
- sequence
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- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000000734 protein sequencing Methods 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 235000004400 serine Nutrition 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
- ZNJHFNUEQDVFCJ-UHFFFAOYSA-M sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid;hydroxide Chemical compound [OH-].[Na+].OCCN1CCN(CCS(O)(=O)=O)CC1 ZNJHFNUEQDVFCJ-UHFFFAOYSA-M 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 235000008521 threonine Nutrition 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 235000002374 tyrosine Nutrition 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 108010005834 tyrosyl-alanyl-glycine Proteins 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 239000012646 vaccine adjuvant Substances 0.000 description 1
- 229940124931 vaccine adjuvant Drugs 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 108010073969 valyllysine Proteins 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 229960004854 viral vaccine Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/16—Otologicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S424/00—Drug, bio-affecting and body treating compositions
- Y10S424/831—Drug, bio-affecting and body treating compositions involving capsular polysaccharide of bacterium, e.g. polyribosyl ribitol phosphate
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/807—Hapten conjugated with peptide or protein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Communicable Diseases (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Oncology (AREA)
- Biophysics (AREA)
- Pulmonology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (15)
1. Nativt PS yttre membranprotein av bakteriestammen Haemopbilus influenzae, vilket kan erhallas geriom ett 5 rengöringsförfarande, soin omfattar ieke—denaturerande dif- ferentialdetergentextraktion av Haemophilus influenzae cel-ler.
2. Protein enligfc patentkrav 1, där näirinda rengö-ringsförfarande omf at tax' ex fraktion av ett föreningsprepa- 1.0· rat hos nämnda cellers yttre membran med en vattenlösning av N-lauroylsarkos natriumsalt, vilken extraktion omfattar steg där: (a.) ett olösligt residuumf vilket omfattar P5 protein, extraheras med en vattenlösning av sulfobetain; 15 (b) det olösliga residuet frän steg (a) upplöses med N-lauroylsarkosinat; (c) residuet frän steg (b) dialyseras med sulfobetain,· {d) det dialyserade extraktet passeras genom en 2 0 anjohbyteskolonn och en katjoiibyteskoionn; (e) PS protein elueras frän en lämplig katjonby-teskolonn, och (f) väsentligen rent PS protein ätervinns frän katjonbyteskolonnen efter elueringen.
3. PS protein enligt patentkrav 1, vilket har se- kVens nr 1 aminosyrasnkvens eller vilken soin heist modif1-erad sekvens därav, där aminosyraresiduer har tillsatts, inlagbs, ersatts eller: avlägsnats litan skada tili protei-nets baktericida eller öpsoniska egenskaper eller bäda, 30 varvid proteinet framkallar baktericida antikroppar mot Haemophilus influenzae bakterie i en värd.
4, För far ande för isolering och rengöring av P5 protein frän Haemophilus influenzae; bakteriens yttre membran efter extrahering av den yttre memhranens förenings-35 preparet med en vattenlösning av N-lauroylsarkös natrium- sait, varvid forfarandet omfattar följahde steg: (a) ett olösligt residuum, vilkefc omfattar P5 protein, extraheras med en yattenlösning av suiföbetain; (b) det olösliga residuet f ran s teg (a) upplöses med M-lauroylsarkosinat;
5. Vaccinsammansättning, som innebäller en immunogen! sk mängd av ett väsentligen rent nativt P5 protain av
15 Haemophilus influenzae bakterie enligt nägot av patentkra-ven 1 - 3 i en farmaceutiskt godtagbar vehikel och i ett valfritt adjuvans.
5 Cc) residuet frän steg (b) dialyseras med sulfohe ta in; (d) det dialyserade extraktet passeras genom en anjonbyteskolonn och en kat jonbyteskolorin; (e) PS protein elueras frän en lämplig katjonby^ 10 teskolonn, och (f) väsentligen rent P5 protein ätervinns frän katjonbyteskolonnen efter elueringen.
6. Vaccinsammansättning enligt patentkrav S, vil-ken ytterligare omfattar ätminstone en tilläggsantigen el- 20 ler en ellen flera organismer.
7- Vaccinsammansättning enligt patentkrav 6, där organismen en bakterie, ett virus, en parasit eller en svamp,
8. Vaccinsammansättning, vilken omfattar en anti-25 genkpnjugat, som bestär väsentligen av ett rent nativt P5 protein enligt nägot av patentkraven 1 - 3, konjugerat i organismens antigen eller i des s epitop eller i en för epitoper farmaceutiskt godtagbar vehikel eller i ett valfritt adjuvans. j
9. Vaccinsammansättning enligt patentkrav 8, där j organismen är Haemophilus influenzae. j
10. Imrnunologisk mängd av ett väsentligen rent na~ j tivt P5 protein av Haemophilus influenzae bakterie enligt j nägot av patentkraven 1 - 3 för anvähdning i immunisering | 35 mot. Haemophilus influenzae; bakterie. j
11. Immunologisk mängd av ett rent nativt P5 protein av Haemophilus influenzae; bakterie enligt nägot av patentkraven 1-3 för användning i immunisering mot Hae-mophl1us inf1uenzae bakterie.
12. Användning enligt patentkrav 11, där proteinet har väsentligen sekvens nr 1 sekvens eller vilken som heist modifierad sekvens därav, där aminosyraresiduer har tiilsatts, inlagts, ersatts eller avlägsnats utan att ska~ da proteinets bakterie idä; eller opsoniska egenskaper eller 10 bäda,:
13. Användning enligt patentkrav 11 eller 12, där proteinet adfninistreras tillsammans med ätminstone en andra antigen av Haemophilus influenzae bakterie.:
14. Immunologisk mängd av ett väsentligen rent na-15 tivt P5 protein av Haemophilus influenzae bakterie, som härstammar frän en etiologisk faktor av otitis media, sinuit: eller kronisk obstruktiv Iungsjukdom, för användning i förebyggande av, behandling av eller minskning av mot-taglighet för otitis, media, sinuit eller kronisk obstruk-20 t iV luhgs j ukdom.
15. Immunologisk mängd av ett väsentligen rent nativt P5 protein av Haemophilus influenzae bakterie, som härstammar f ran en etiologisk faktor av otitis media, sinuit eller kronisk obstruktiv iungsjukdom, för användning 25. framställning av ett läkemedel för förebyggande av, behandling av eller minskning av mottaglighet för otitis media, sinuit eller kronisk obstruktiv Iungsjukdom, j j
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/210,394 US5770213A (en) | 1994-05-05 | 1994-05-05 | Purified nontypable haemophilus influenzae P5 protein as a vaccine for nontypable haemophilus influenzae infection |
| US21039494 | 1994-05-05 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI952144A0 FI952144A0 (sv) | 1995-05-04 |
| FI952144A FI952144A (sv) | 1995-11-06 |
| FI120149B true FI120149B (sv) | 2009-07-15 |
Family
ID=22782738
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI952144A FI120149B (sv) | 1994-05-05 | 1995-05-04 | Renat P5 protein av icke-typbar Haemophilus influenzae som vaccin mot en icke-typbar Haemophilus influenzae -stam |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US5770213A (sv) |
| EP (1) | EP0680765B1 (sv) |
| JP (2) | JP4236215B2 (sv) |
| KR (1) | KR100366482B1 (sv) |
| AT (1) | ATE356633T1 (sv) |
| AU (1) | AU704882B2 (sv) |
| CA (1) | CA2148563C (sv) |
| DE (1) | DE69535422T2 (sv) |
| DK (1) | DK0680765T3 (sv) |
| ES (1) | ES2282992T3 (sv) |
| FI (1) | FI120149B (sv) |
| IL (1) | IL113595A (sv) |
| NO (1) | NO951750L (sv) |
| PT (1) | PT680765E (sv) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002089839A1 (en) * | 2001-05-03 | 2002-11-14 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Intranasal immunization with detoxified lipooligosaccharide from nontypeable haemophilus influenzae or moraxella catarrhalis |
| US20040126381A1 (en) * | 1996-04-23 | 2004-07-01 | Xin-Xing Gu | Intranasal immunization with detoxified lipooligosaccharide from nontypeable haemophilus influenzae or moraxella |
| GB9812613D0 (en) * | 1998-06-11 | 1998-08-12 | Smithkline Beecham Biolog | Vaccine |
| US6974581B1 (en) * | 1998-12-15 | 2005-12-13 | Aventis Pasteur Limited | Multi-component vaccine comprising at least two antigens from haemophilus influenzae to protect against disease |
| GB9904183D0 (en) * | 1999-02-24 | 1999-04-14 | Smithkline Beecham Biolog | Novel compounds |
| US7101989B1 (en) | 1999-07-09 | 2006-09-05 | University Of North Carolina At Chapel Hill | DsrA protein and polynucleotides encoding the same |
| GB0025486D0 (en) * | 2000-10-17 | 2000-11-29 | Smithkline Beecham Biolog | Novel compounds |
| WO2004009621A2 (en) * | 2002-07-24 | 2004-01-29 | Id Biomedical Corporation | Polypeptides of nontypeable haemophilus influenzae |
| US7709001B2 (en) | 2005-04-08 | 2010-05-04 | Wyeth Llc | Multivalent pneumococcal polysaccharide-protein conjugate composition |
| ME01334B (me) | 2005-04-08 | 2013-12-20 | Wyeth Llc | Polivalentni pripravak konjugata pneumokoknog polisaharida s proteinom |
| JP2012502898A (ja) | 2008-09-17 | 2012-02-02 | ハンター・イミュノロジー・リミテッド | 分類不能（ｎｏｎ−ｔｙｐｅａｂｌｅ）インフルエンザ菌（Ｈａｅｍｏｐｈｉｌｕｓｉｎｆｌｕｅｎｚａｅ）ワクチンおよびその使用 |
| KR102409709B1 (ko) | 2021-11-30 | 2022-06-16 | 박정민 | 케이블 접속장치 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5300632A (en) * | 1986-11-18 | 1994-04-05 | Research Foundation Of State University Of New York | Method for purifying an outer membrane protein of Haemophilus influenzae |
| US5098997A (en) * | 1987-12-11 | 1992-03-24 | Praxis Biologics, Inc. | Vaccines for Haemophilus influenzae |
| US5098987A (en) * | 1989-07-13 | 1992-03-24 | The United States Of America As Represented By The Secretary Of The Air Force | Crosslinkable rigid-rod benzobisazole polymer |
| WO1994026304A1 (en) | 1993-05-18 | 1994-11-24 | Ohio State Research Foundation | Otitis media vaccine |
-
1994
- 1994-05-05 US US08/210,394 patent/US5770213A/en not_active Ceased
-
1995
- 1995-05-02 EP EP95302996A patent/EP0680765B1/en not_active Expired - Lifetime
- 1995-05-02 ES ES95302996T patent/ES2282992T3/es not_active Expired - Lifetime
- 1995-05-02 AT AT95302996T patent/ATE356633T1/de not_active IP Right Cessation
- 1995-05-02 PT PT95302996T patent/PT680765E/pt unknown
- 1995-05-02 DK DK95302996T patent/DK0680765T3/da active
- 1995-05-02 DE DE69535422T patent/DE69535422T2/de not_active Expired - Lifetime
- 1995-05-02 JP JP13295495A patent/JP4236215B2/ja not_active Expired - Fee Related
- 1995-05-03 AU AU17814/95A patent/AU704882B2/en not_active Ceased
- 1995-05-03 CA CA002148563A patent/CA2148563C/en not_active Expired - Fee Related
- 1995-05-03 IL IL11359595A patent/IL113595A/en not_active IP Right Cessation
- 1995-05-04 FI FI952144A patent/FI120149B/sv active IP Right Grant
- 1995-05-04 KR KR1019950011163A patent/KR100366482B1/ko not_active IP Right Cessation
- 1995-05-04 NO NO951750A patent/NO951750L/no unknown
-
2000
- 2000-06-21 US US09/599,652 patent/USRE37741E1/en not_active Expired - Lifetime
-
2008
- 2008-10-24 JP JP2008274435A patent/JP2009051859A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US5770213A (en) | 1998-06-23 |
| IL113595A (en) | 2001-05-20 |
| FI952144A (sv) | 1995-11-06 |
| DE69535422D1 (de) | 2007-04-26 |
| CA2148563A1 (en) | 1995-11-06 |
| DK0680765T3 (da) | 2007-07-02 |
| EP0680765B1 (en) | 2007-03-14 |
| AU704882B2 (en) | 1999-05-06 |
| JP4236215B2 (ja) | 2009-03-11 |
| ES2282992T3 (es) | 2007-10-16 |
| USRE37741E1 (en) | 2002-06-11 |
| IL113595A0 (en) | 1995-08-31 |
| PT680765E (pt) | 2007-05-31 |
| ATE356633T1 (de) | 2007-04-15 |
| NO951750D0 (no) | 1995-05-04 |
| FI952144A0 (sv) | 1995-05-04 |
| CA2148563C (en) | 2008-10-07 |
| DE69535422T2 (de) | 2007-12-13 |
| EP0680765A1 (en) | 1995-11-08 |
| KR950031108A (ko) | 1995-12-18 |
| JP2009051859A (ja) | 2009-03-12 |
| KR100366482B1 (ko) | 2003-08-02 |
| JPH08337598A (ja) | 1996-12-24 |
| NO951750L (no) | 1995-11-06 |
| AU1781495A (en) | 1995-11-16 |
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