FI116848B - Xylanas erhållen ur en Bacillusart, expressionsvektorer för sådana xylanaser och andra proteiner, värdorganismer för och användning av dessa - Google Patents

Xylanas erhållen ur en Bacillusart, expressionsvektorer för sådana xylanaser och andra proteiner, värdorganismer för och användning av dessa Download PDF

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Publication number
FI116848B
FI116848B FI943389A FI943389A FI116848B FI 116848 B FI116848 B FI 116848B FI 943389 A FI943389 A FI 943389A FI 943389 A FI943389 A FI 943389A FI 116848 B FI116848 B FI 116848B
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xylanase
seq
plasmid
sequence
bacillus
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FI943389A
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English (en)
Finnish (fi)
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FI943389A (sv
FI943389A0 (sv
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Antoine Amory
Roman Vetter
Pierre Ledoux
Buyl Eric De
Rene Detroz
Andree Lahaye
Christophe Andre
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Genencor Int
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Paper (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (19)

1. Renad xylanas, kännetecknad den har aminosyrasekvensen enligt sekvensidentif 5 nuiraner 24.
2. Renad xylanas enligt patentkrav 1, k ä tecknad av att den dessutom omfattar en 1 yens, som har aminosyrasekvensen enligt sekven fieringsnummer 25.
3. Xylanas enligt patentkrav 1 eller 2, netecknad av att den härstammar frän Baci milus PRL Bl2-stammen.
4. Förfarande for att producera xylanas : cillus-species, kännetecknat av att E 15 species transformeras med en nukleotidsekvens, s· för en xylanas, som omfattar aminosyrasekvenser sekvensidentifieringsnummer 24, Bacillus-species lämpliga förhällanden för expression av xylanasen lanasen tillvaratas frän odlingen. 20 5. Förfarande enligt patentkrav 4, k ä tecknat av att Bacillus är en transformerad · Bacillus licheniformis. • · V.* 6. Förfarande enligt patentkrav 4 eller 5, Γ·*: netecknat av att xylanasen härstammar frä ·*·*: 25 lus pumilus PRL B12 och den produceras heterologis • * mikroorganism av slaktet Bacillus, som uppviss * » »* naturen förekommande gen som kodar för alkalisk pr « » I
7. Förfarande enligt patentkrav 6, k ä .. tecknat av att frän mikroorganismen har av • · · 121
9. DNA-molekyl enligt patentkrav 8, k ä tecknad av att nukleotidsekvensen kodar f xylanas, som har aminosyrasekvensen enligt sekver fieringsnummer 26.
10. DNA-molekyl enligt patentkrav 8 e kännetecknad av att den dessutom innef nukleotidsekvens enligt sekvensidentifieringsnumme kodar för presekvensen av xylanasen av B. Pumilus
11. DNA-molekyl enligt nägot av patentkr; 10 10, kännetecknad av att den dessut halier uppströms nukleotidsekvenser av xylanasgen Pumilus PRL B12, sasom visas i sekvensen med ic ringsnummer 28.
12. DNA-molekyl enligt patentkrav 11, k ä 15 tecknad av att den omfattar en isolerad x^ av B. Pumilus PRL B12, som har sekvensidentifierir 1.
13. Expressionsvektor, kännetec av att den innehäller en DNA-molekyl enligt nago' 20 tentkraven 8-12.
14. Expressionsvektor enligt patentki V : kännetecknad av att den är expressic :.V pUB-BPX12 enligt figur 4. M *
15. Expressionsvektor enligt patentki 25 kännetecknad av att den är expressic ·;. pUBDEBRAl enligt figur 5. ·*·»
16. Expressionsvektor enligt patentki * # kännetecknad av att den är expressic • . pKACl enligt figur 6. *··.* 30 17. Expressionsvektor enligt patentki • · 1 kännetecknad av att den är expressic 122
19. Expressionsvektor enligt patentki kännetecknad av att den är expressio pL7TAKA enligt figur 9.
20. Bacillus licheniformis, kannet 5 n a d av att den har transformerats med express torn enligt patentkrav 13.
21. Bacillus licheniformis enligt patent] kännetecknad av att den har transforme DNA-molekylen enligt patentkrav 9, och fran den 10 lägsnats DNA-sekvensen som kodar för alkalisk prot
22. Bacillus licheniformis enligt patent] kännetecknad av att den är Bacillus formis SE2, LGM P-14034 och mutanter och varianter
23. Förfarande för behandling av lignoce 15 haltig massa, kännetecknat av att t behandlas med en xylanas enligt nägot av patentkr. 3.
24. Förfarande enligt patentkrav 23, k ä tecknat av att förfarandet omfattar förbe 20 av den lignocellulosahaltiga massan med xylanasen. Ml « 9 · 9 > · • * ♦ * » » • · ·· ♦ ·· 9 • * • 9 · · 9 9« • · • · ♦ «« 9999 ♦ ·» 9 9« 9 9| • * 9 9« ♦ · · 999 « *·· • « • · • 9 ·
FI943389A 1993-07-15 1994-07-15 Xylanas erhållen ur en Bacillusart, expressionsvektorer för sådana xylanaser och andra proteiner, värdorganismer för och användning av dessa FI116848B (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9314780A GB2279955B (en) 1993-07-15 1993-07-15 Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
GB9314780 1993-07-15

Publications (3)

Publication Number Publication Date
FI943389A0 FI943389A0 (sv) 1994-07-15
FI943389A FI943389A (sv) 1995-01-16
FI116848B true FI116848B (sv) 2006-03-15

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FI943389A FI116848B (sv) 1993-07-15 1994-07-15 Xylanas erhållen ur en Bacillusart, expressionsvektorer för sådana xylanaser och andra proteiner, värdorganismer för och användning av dessa

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US (3) US6180382B1 (sv)
EP (1) EP0634490B1 (sv)
JP (1) JPH0767637A (sv)
AU (1) AU687808B2 (sv)
BR (1) BR9402834A (sv)
CA (1) CA2128050C (sv)
DE (1) DE69428238T2 (sv)
DK (1) DK0634490T3 (sv)
FI (1) FI116848B (sv)
GB (1) GB2279955B (sv)
MX (1) MX9405386A (sv)
NO (1) NO942652L (sv)
NZ (1) NZ260989A (sv)

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EP1995319B1 (en) * 2002-04-10 2011-08-31 Novozymes A/S Bacillus licheniformis mutant host cell
AU2003214036A1 (en) * 2002-04-10 2003-10-27 Novozymes A/S Bacillus licheniformis mutant host cell
KR20050010053A (ko) * 2002-06-14 2005-01-26 다이버사 코포레이션 자일라나제, 이를 암호화하는 핵산, 및 이의 제조 및 사용방법
WO2006047713A2 (en) * 2004-10-27 2006-05-04 Novozymes North America, Inc. Enzymatic-assisted chlorine dioxide treatment
BRPI0520253B1 (pt) * 2005-05-04 2016-09-06 Novozymes As métodos para a deslignificação e/ou alvejamento de uma polpa, e para o tratamento de água de rejeito e/ou lama
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US9732330B2 (en) 2008-01-25 2017-08-15 Battelle Energy Alliance, Llc Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
US8492114B2 (en) * 2008-01-25 2013-07-23 Battelle Energy Alliance, Llc Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
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JP2011510657A (ja) 2008-01-31 2011-04-07 バテル エナジー アライアンス，エルエルシー アリサイクロバチルス・アシドカルダリウスおよび関連生物体に由来する好熱性および好熱好酸性バイオポリマー分解遺伝子および酵素、方法
US8557557B2 (en) * 2008-01-31 2013-10-15 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
US8426185B2 (en) 2008-01-31 2013-04-23 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
US8497110B2 (en) 2008-01-31 2013-07-30 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
BRPI0907407A2 (pt) 2008-02-22 2019-09-24 Battelle Energy Alliance Llc controle transcricional em alicyclobacillus acidocaldarius e genes, proteínas e métodos associados
CN102105588A (zh) 2008-02-26 2011-06-22 巴特勒能源同盟有限公司 来自酸热脂环酸杆菌的嗜热的与嗜热嗜酸的糖转运蛋白的基因和酶以及相关生物、方法
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Publication number Publication date
JPH0767637A (ja) 1995-03-14
FI943389A (sv) 1995-01-16
AU687808B2 (en) 1998-03-05
NO942652D0 (no) 1994-07-14
GB2279955A (en) 1995-01-18
US6423523B1 (en) 2002-07-23
GB9314780D0 (en) 1993-08-25
NZ260989A (en) 1995-08-28
CA2128050A1 (en) 1995-01-16
CA2128050C (en) 2006-12-19
MX9405386A (es) 1995-01-31
GB2279955B (en) 1998-02-18
NO942652L (no) 1995-01-16
EP0634490A1 (en) 1995-01-18
DE69428238T2 (de) 2002-06-27
US6180382B1 (en) 2001-01-30
DK0634490T3 (da) 2002-01-14
AU6743294A (en) 1995-01-27
DE69428238D1 (de) 2001-10-18
US6426211B1 (en) 2002-07-30
FI943389A0 (sv) 1994-07-15
EP0634490B1 (en) 2001-09-12
BR9402834A (pt) 1995-06-13

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