FI109361B - Mikrobinen proteiinin ilmentämisjärjestelmä - Google Patents

Mikrobinen proteiinin ilmentämisjärjestelmä Download PDF

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Publication number
FI109361B
FI109361B FI991014A FI991014A FI109361B FI 109361 B FI109361 B FI 109361B FI 991014 A FI991014 A FI 991014A FI 991014 A FI991014 A FI 991014A FI 109361 B FI109361 B FI 109361B
Authority
FI
Finland
Prior art keywords
protein
proteins
cafl
periplasmic
hil
Prior art date
Application number
FI991014A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI991014A0 (fi
FI991014A (fi
Inventor
Timo Kalevi Korpela
Yalov Vladimir Petrovich Zav
Sheila Macintyre-Ayane
Anton Vladimirovich Zavialov
Natalia Vsevolodov Battchikova
Lada Evgenievna Petrovskaya
Vyacheslav Grigorievic Korobko
Original Assignee
Korpela Timo
Yalov Vladimir Petrovich Zav
Sheila Macintyre-Ayane
Anton Vladimirovich Zavialov
Natalia Vsevolodov Battchikova
Lada Evgenievna Petrovskaya
Vyacheslav Grigorievic Korobko
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korpela Timo, Yalov Vladimir Petrovich Zav, Sheila Macintyre-Ayane, Anton Vladimirovich Zavialov, Natalia Vsevolodov Battchikova, Lada Evgenievna Petrovskaya, Vyacheslav Grigorievic Korobko filed Critical Korpela Timo
Publication of FI991014A0 publication Critical patent/FI991014A0/fi
Priority to FI991014A priority Critical patent/FI109361B/fi
Priority to AU43003/00A priority patent/AU777246B2/en
Priority to NZ515483A priority patent/NZ515483A/en
Priority to ES00922689T priority patent/ES2245306T3/es
Priority to PT00922689T priority patent/PT1173592E/pt
Priority to US09/959,650 priority patent/US6919198B1/en
Priority to DK00922689T priority patent/DK1173592T3/da
Priority to DE60021719T priority patent/DE60021719T2/de
Priority to CA002370436A priority patent/CA2370436A1/en
Priority to AT00922689T priority patent/ATE301200T1/de
Priority to PCT/FI2000/000387 priority patent/WO2000066756A1/en
Priority to EP00922689A priority patent/EP1173592B1/en
Publication of FI991014A publication Critical patent/FI991014A/fi
Priority to ZA200109231A priority patent/ZA200109231B/en
Application granted granted Critical
Publication of FI109361B publication Critical patent/FI109361B/fi

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FI991014A 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä FI109361B (fi)

Priority Applications (13)

Application Number Priority Date Filing Date Title
FI991014A FI109361B (fi) 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä
DK00922689T DK1173592T3 (da) 1999-05-04 2000-05-03 Mikrobielt proteinekspressionssystem
CA002370436A CA2370436A1 (en) 1999-05-04 2000-05-03 Microbial protein expression system
ES00922689T ES2245306T3 (es) 1999-05-04 2000-05-03 Sistema de expresion de proteina microbiana.
PT00922689T PT1173592E (pt) 1999-05-04 2000-05-03 Sistema de expressao da proteina microbiana
US09/959,650 US6919198B1 (en) 1999-05-04 2000-05-03 Microbial protein expression system
AU43003/00A AU777246B2 (en) 1999-05-04 2000-05-03 Microbial protein expression system
DE60021719T DE60021719T2 (de) 1999-05-04 2000-05-03 Mikrobielle proteinexpressionssysteme
NZ515483A NZ515483A (en) 1999-05-04 2000-05-03 Microbial protein expression system for secretion of a recombinant heterologous protein into periplasm of bacteria
AT00922689T ATE301200T1 (de) 1999-05-04 2000-05-03 Mikrobielle proteinexpressionssysteme
PCT/FI2000/000387 WO2000066756A1 (en) 1999-05-04 2000-05-03 Microbial protein expression system
EP00922689A EP1173592B1 (en) 1999-05-04 2000-05-03 Microbial protein expression system
ZA200109231A ZA200109231B (en) 1999-05-04 2001-11-08 Microbial protein expression system.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI991014 1999-05-04
FI991014A FI109361B (fi) 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä

Publications (3)

Publication Number Publication Date
FI991014A0 FI991014A0 (fi) 1999-05-04
FI991014A FI991014A (fi) 2000-11-05
FI109361B true FI109361B (fi) 2002-07-15

Family

ID=8554586

Family Applications (1)

Application Number Title Priority Date Filing Date
FI991014A FI109361B (fi) 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä

Country Status (13)

Country Link
US (1) US6919198B1 (es)
EP (1) EP1173592B1 (es)
AT (1) ATE301200T1 (es)
AU (1) AU777246B2 (es)
CA (1) CA2370436A1 (es)
DE (1) DE60021719T2 (es)
DK (1) DK1173592T3 (es)
ES (1) ES2245306T3 (es)
FI (1) FI109361B (es)
NZ (1) NZ515483A (es)
PT (1) PT1173592E (es)
WO (1) WO2000066756A1 (es)
ZA (1) ZA200109231B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
US6677139B1 (en) 1999-12-23 2004-01-13 Genecor International, Inc. Methods for production of proteins in host cells
DE60141769D1 (de) 2000-12-26 2010-05-20 Sekisui Chemical Co Ltd Verfahren zur herstellung von rekombinantem protein und fusionsprotein
ATE512230T1 (de) 2004-08-18 2011-06-15 Univ Alberta Proteinherstellungsverfahren unter nutzung von yebf
EP1811026B1 (en) 2004-10-15 2015-01-28 Sekisui Chemical Co., Ltd. Method of immunizing animal, composition for immunization, method of producing antibody, method of producing hybridoma and method of producing monoclonal antibody
EP2858675A2 (en) * 2012-06-11 2015-04-15 University Of Newcastle Upon Tyne Recombinant polypeptide
CN110724187B (zh) * 2018-07-16 2022-09-16 甘李药业股份有限公司 一种高效表达利拉鲁肽前体的重组工程菌及其应用
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0663010T3 (da) * 1992-10-02 2000-11-13 Res Corp Technologies Inc Fremgangsmåde til forøgelse af sekretion af overudtrykte proteiner
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
JPH09173078A (ja) * 1995-09-14 1997-07-08 Tadayuki Imanaka 分子シャペロンを用いる蛋白質の製造方法
JP3344618B2 (ja) * 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 シャペロン発現プラスミド

Also Published As

Publication number Publication date
DE60021719T2 (de) 2006-06-08
EP1173592B1 (en) 2005-08-03
WO2000066756A1 (en) 2000-11-09
ZA200109231B (en) 2002-11-08
NZ515483A (en) 2003-09-26
AU777246B2 (en) 2004-10-07
PT1173592E (pt) 2005-11-30
FI991014A0 (fi) 1999-05-04
FI991014A (fi) 2000-11-05
EP1173592A1 (en) 2002-01-23
AU4300300A (en) 2000-11-17
CA2370436A1 (en) 2000-11-09
ES2245306T3 (es) 2006-01-01
DE60021719D1 (de) 2005-09-08
US6919198B1 (en) 2005-07-19
DK1173592T3 (da) 2005-10-10
ATE301200T1 (de) 2005-08-15

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