FI991014A - Mikrobinen proteiinin ilmentämisjärjestelmä - Google Patents

Mikrobinen proteiinin ilmentämisjärjestelmä Download PDF

Info

Publication number
FI991014A
FI991014A FI991014A FI991014A FI991014A FI 991014 A FI991014 A FI 991014A FI 991014 A FI991014 A FI 991014A FI 991014 A FI991014 A FI 991014A FI 991014 A FI991014 A FI 991014A
Authority
FI
Finland
Prior art keywords
caf1
mature
signal peptide
protein
subunit
Prior art date
Application number
FI991014A
Other languages
English (en)
Swedish (sv)
Other versions
FI991014A0 (fi
FI109361B (fi
Inventor
Sheila Macintyre-Ayane
Anton Vladimirovich Zavialov
Natalia Vsevolodov Battchikova
Lada Evgenievna Petrovskaya
Vyacheslav Grigorievic Korobko
Yalov Vladimir Petrovich Zav
Timo Kalevi Korpela
Original Assignee
Macintyre Ayane Sheila
Anton Vladimirovich Zavialov
Natalia Vsevolodov Battchikova
Lada Evgenievna Petrovskaya
Vyacheslav Grigorievic Korobko
Yalov Vladimir Petrovich Zav
Timo Kalevi Korpela
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Macintyre Ayane Sheila, Anton Vladimirovich Zavialov, Natalia Vsevolodov Battchikova, Lada Evgenievna Petrovskaya, Vyacheslav Grigorievic Korobko, Yalov Vladimir Petrovich Zav, Timo Kalevi Korpela filed Critical Macintyre Ayane Sheila
Publication of FI991014A0 publication Critical patent/FI991014A0/fi
Priority to FI991014A priority Critical patent/FI109361B/fi
Priority to ES00922689T priority patent/ES2245306T3/es
Priority to DK00922689T priority patent/DK1173592T3/da
Priority to PT00922689T priority patent/PT1173592E/pt
Priority to CA002370436A priority patent/CA2370436A1/en
Priority to DE60021719T priority patent/DE60021719T2/de
Priority to US09/959,650 priority patent/US6919198B1/en
Priority to EP00922689A priority patent/EP1173592B1/en
Priority to PCT/FI2000/000387 priority patent/WO2000066756A1/en
Priority to AU43003/00A priority patent/AU777246B2/en
Priority to AT00922689T priority patent/ATE301200T1/de
Priority to NZ515483A priority patent/NZ515483A/en
Publication of FI991014A publication Critical patent/FI991014A/fi
Priority to ZA200109231A priority patent/ZA200109231B/en
Application granted granted Critical
Publication of FI109361B publication Critical patent/FI109361B/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FI991014A 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä FI109361B (fi)

Priority Applications (13)

Application Number Priority Date Filing Date Title
FI991014A FI109361B (fi) 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä
US09/959,650 US6919198B1 (en) 1999-05-04 2000-05-03 Microbial protein expression system
PCT/FI2000/000387 WO2000066756A1 (en) 1999-05-04 2000-05-03 Microbial protein expression system
PT00922689T PT1173592E (pt) 1999-05-04 2000-05-03 Sistema de expressao da proteina microbiana
CA002370436A CA2370436A1 (en) 1999-05-04 2000-05-03 Microbial protein expression system
DE60021719T DE60021719T2 (de) 1999-05-04 2000-05-03 Mikrobielle proteinexpressionssysteme
ES00922689T ES2245306T3 (es) 1999-05-04 2000-05-03 Sistema de expresion de proteina microbiana.
EP00922689A EP1173592B1 (en) 1999-05-04 2000-05-03 Microbial protein expression system
DK00922689T DK1173592T3 (da) 1999-05-04 2000-05-03 Mikrobielt proteinekspressionssystem
AU43003/00A AU777246B2 (en) 1999-05-04 2000-05-03 Microbial protein expression system
AT00922689T ATE301200T1 (de) 1999-05-04 2000-05-03 Mikrobielle proteinexpressionssysteme
NZ515483A NZ515483A (en) 1999-05-04 2000-05-03 Microbial protein expression system for secretion of a recombinant heterologous protein into periplasm of bacteria
ZA200109231A ZA200109231B (en) 1999-05-04 2001-11-08 Microbial protein expression system.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI991014 1999-05-04
FI991014A FI109361B (fi) 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä

Publications (3)

Publication Number Publication Date
FI991014A0 FI991014A0 (fi) 1999-05-04
FI991014A true FI991014A (fi) 2000-11-05
FI109361B FI109361B (fi) 2002-07-15

Family

ID=8554586

Family Applications (1)

Application Number Title Priority Date Filing Date
FI991014A FI109361B (fi) 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä

Country Status (13)

Country Link
US (1) US6919198B1 (fi)
EP (1) EP1173592B1 (fi)
AT (1) ATE301200T1 (fi)
AU (1) AU777246B2 (fi)
CA (1) CA2370436A1 (fi)
DE (1) DE60021719T2 (fi)
DK (1) DK1173592T3 (fi)
ES (1) ES2245306T3 (fi)
FI (1) FI109361B (fi)
NZ (1) NZ515483A (fi)
PT (1) PT1173592E (fi)
WO (1) WO2000066756A1 (fi)
ZA (1) ZA200109231B (fi)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
US6677139B1 (en) 1999-12-23 2004-01-13 Genecor International, Inc. Methods for production of proteins in host cells
KR100892889B1 (ko) 2000-12-26 2009-04-15 세키스이가가쿠 고교가부시키가이샤 재조합 단백질의 생산방법 및 융합 단백질
ATE512230T1 (de) * 2004-08-18 2011-06-15 Univ Alberta Proteinherstellungsverfahren unter nutzung von yebf
JP4644680B2 (ja) 2004-10-15 2011-03-02 積水化学工業株式会社 動物の免疫方法、免疫用組成物、抗体の製造方法、ハイブリドーマの製造方法、及びモノクローナル抗体の製造方法
CN104703627A (zh) * 2012-06-11 2015-06-10 泰恩河上的纽卡斯尔大学 分子伴侣/引领蛋白融合蛋白
CN110724187B (zh) * 2018-07-16 2022-09-16 甘李药业股份有限公司 一种高效表达利拉鲁肽前体的重组工程菌及其应用
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994008012A1 (en) * 1992-10-02 1994-04-14 Research Corporation Technologies, Inc. Methods for increasing secretion of overexpressed proteins
US5639635A (en) 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
JPH09173078A (ja) * 1995-09-14 1997-07-08 Tadayuki Imanaka 分子シャペロンを用いる蛋白質の製造方法
JP3344618B2 (ja) * 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 シャペロン発現プラスミド

Also Published As

Publication number Publication date
AU4300300A (en) 2000-11-17
NZ515483A (en) 2003-09-26
US6919198B1 (en) 2005-07-19
EP1173592A1 (en) 2002-01-23
DE60021719T2 (de) 2006-06-08
FI991014A0 (fi) 1999-05-04
ATE301200T1 (de) 2005-08-15
AU777246B2 (en) 2004-10-07
PT1173592E (pt) 2005-11-30
DK1173592T3 (da) 2005-10-10
WO2000066756A1 (en) 2000-11-09
EP1173592B1 (en) 2005-08-03
ZA200109231B (en) 2002-11-08
FI109361B (fi) 2002-07-15
DE60021719D1 (de) 2005-09-08
ES2245306T3 (es) 2006-01-01
CA2370436A1 (en) 2000-11-09

Similar Documents

Publication Publication Date Title
DK1173592T3 (da) Mikrobielt proteinekspressionssystem
HUP9800730A2 (hu) Expressziós rendszer heterológ antigének fúziós proteinként történő expresszáltatására
IL157414A0 (en) Fusion protein for the secretion of a protein of interest into the supernatant of the bacterial culture
IT1270123B (it) Composizioni farmaceutiche contenenti microorganismi ingegnerizzati e loro uso per terapia
ATE312932T1 (de) Zwillingsarginin translokation in bacillus
DK1356073T3 (da) Fermenteringsfremgangsmåde til fremstilling af heterologe genprodukter i mælkesyrebakterier
CA2033176A1 (en) Growth hormone fusion proteins
WO1993023434A3 (en) Bpi-immunoglobulin fusion proteins
DE69412593T2 (de) Anthrax-toxin-fusionsproteine und deren verwendungen
CY1114413T1 (el) Πρωτεϊνικη μετατοπiση σε φυτικα κυτταρα
ATE251464T1 (de) Verwendung natriuretischer peptide als antibiotisch wirksame substanzen zur behandlung von bakteriellen infektionen
AUPN442295A0 (en) Regulated autocrine growth of mammalian cells
CY1106681T1 (el) Διεργασια παραγωγης του ανασυνδyασμενου πλακουντιου αυξητικου παραγοντα
CA2385287A1 (en) Signal sequences for preparing leu-hirudin by secretion by e. coli into the culture medium
EE200000102A (et) Meetodid ja kompositsioonid teraapiaks, kasutades sekreteeritavaid valke, näiteks interferoon-beetat kodeerivaid geene
AU590861B2 (en) New human lymphotoxin (TNF-beta) polypeptides their prepartion and use
GB0116460D0 (en) Biological products
Honjo et al. Construction of the secretion vector containing the prepro-structure coding region of Bacillus amyloliquefaciens neutral protease gene and secretion of Bacillus subtilis α-amylase and human interferon-beta in Bacillus subtilis
EP1678308A4 (en) EXPRESSION VECTOR FOR SECRETING AN ANTIBODY FRAGMENT FROM AN E. COLI SIGNAL SEQUENCE AND METHOD FOR THE SERIAL PRODUCTION OF THE ANTIBODY FRAGMENT
WO2003004599A3 (en) Methods of making and using expression constructs for the expression of recombinant proteins
Lundell et al. Cytoplasmic and periplasmic expression of a highly basic protein, human interleukin 4, in Escherichia coli
Boos et al. The maltose system
KR20060006204A (ko) 비브리오 패혈증균의 ｐａｓ 인자를 이용한 재조합 융합단백질의 생산 방법
EP1132398A3 (en) DNA sequence encoding the specific and antigenic outer membrane protein of salmonella typhi
WO2001044278A3 (en) Antigen preparations

Legal Events

Date Code Title Description
MA Patent expired