ES8705976A1 - Un metodo para detectar una interaccion ligante - Google Patents
Un metodo para detectar una interaccion liganteInfo
- Publication number
- ES8705976A1 ES8705976A1 ES549950A ES549950A ES8705976A1 ES 8705976 A1 ES8705976 A1 ES 8705976A1 ES 549950 A ES549950 A ES 549950A ES 549950 A ES549950 A ES 549950A ES 8705976 A1 ES8705976 A1 ES 8705976A1
- Authority
- ES
- Spain
- Prior art keywords
- disclosed
- nucleic acid
- separation step
- requiring
- coelenterate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
METODO PARA DETECTAR UNA INTERACCION LIGANTE. CONSISTE EN: A) PONER EN CONTACTO CON UN MEDIO REACCIONAL EL PRIMER LIGANTE CON UNA CANTIDAD DEL SEGUNDO LIGANTE QUE ES IGUAL AL LIGANTE QUE HA DE DETECTARSE, SIENDO EL SEGUNDO LIGANTE ENLAZADO COVALENTEMENTE CON UNA MARCA PROTEINICA ELEGIDA ENTRE FOTOPROTEINAS, LUCIFERASA Y APOFOTOPROTEINAS DERIVADAS DE COELENTERATO, Y CON LA MUESTRA CON LA QUE SE FORMA UN PRIMER COMPLEJO ENTRE EL PRIMER Y SEGUNDO LIGANTES, Y FORMAR UN SEGUNDO COMPLEJO ENTRE EL PRIMER LIGANTE Y EL LIGANTE QUE HA DE DETECTARSE; B) SEPARAR DE LA MEZCLA REACCIONAL AMBOS COMPLEJOS; C) PONER EN CONTACTO UN REACTIVO DISPARADOR DE LUZ CON AMBOS COMPLEJOS PARA EMITIR LUZ; Y D) DETECTAR LA LUZ EMITIDA POR LA MOLECULA DE LUCIFERINA. TIENE APLICACION PARA FINES DE DIAGNOSTICO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US54140583A | 1983-10-13 | 1983-10-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES549950A0 ES549950A0 (es) | 1987-05-16 |
ES8705976A1 true ES8705976A1 (es) | 1987-05-16 |
Family
ID=24159462
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES536680A Expired ES8607563A1 (es) | 1983-10-13 | 1984-10-11 | Un metodo para detectar una interaccion ligante |
ES549950A Expired ES8705976A1 (es) | 1983-10-13 | 1985-12-16 | Un metodo para detectar una interaccion ligante |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES536680A Expired ES8607563A1 (es) | 1983-10-13 | 1984-10-11 | Un metodo para detectar una interaccion ligante |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0137515A3 (es) |
JP (1) | JPS6122254A (es) |
AU (1) | AU590511B2 (es) |
CA (1) | CA1234756A (es) |
DK (1) | DK487784A (es) |
ES (2) | ES8607563A1 (es) |
FI (1) | FI844017L (es) |
NO (1) | NO164442C (es) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4820630A (en) * | 1984-11-23 | 1989-04-11 | Digene Diagnostics, Incorporated | Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels |
JP3293820B2 (ja) * | 1985-12-13 | 2002-06-17 | エンゾ− バイオケム インコ−ポレイテツド | 標的ポリヌクレオチドにハイブリツド形成するための新規な一工程方法とポリヌクレオチド化合物 |
GB8610426D0 (en) * | 1986-04-29 | 1986-06-04 | Leaback D H | Bound assay components |
JPS62261942A (ja) * | 1986-05-08 | 1987-11-14 | Chisso Corp | 金属の検出方法 |
FR2617974B1 (fr) * | 1987-07-07 | 1992-11-13 | Stabiligen | Procede de dosage immunologique par bio- ou chimio- luminescence |
AU3342689A (en) * | 1988-03-24 | 1989-10-16 | Igen Incorporated | Luminescent chimeric proteins |
ATE123033T1 (de) * | 1989-03-22 | 1995-06-15 | Nippon Zoki Pharmaceutical Co | Luciferinderivate. |
FI901681A0 (fi) * | 1989-04-10 | 1990-04-03 | Ela Technologies Inc | Foerfarande foer oekning av kaensligheten hos luminescensanalyser. |
FI901680A0 (fi) * | 1989-04-10 | 1990-04-03 | Univ Georgia | Enzymluminescensanalys. |
US5683888A (en) | 1989-07-22 | 1997-11-04 | University Of Wales College Of Medicine | Modified bioluminescent proteins and their use |
GB8916806D0 (en) * | 1989-07-22 | 1989-09-06 | Univ Wales Medicine | Modified proteins |
JP3058673B2 (ja) * | 1989-11-10 | 2000-07-04 | 株式会社明電舎 | サイトカインの測定方法及びその測定用キット |
JPH04149199A (ja) * | 1990-10-11 | 1992-05-22 | Chisso Corp | 発光蛋白エクオリン標識抗体 |
BE1006179A3 (fr) * | 1992-09-22 | 1994-05-31 | Lambdatech S A | Conjugue destine a la detection et/ou au dosage d'un compose biologique. |
EP0683822B1 (en) * | 1993-02-12 | 2002-05-02 | Sealite Sciences, Inc. | Preparation of photoprotein conjugates and methods of use thereof |
US6576419B1 (en) | 1993-07-23 | 2003-06-10 | University Of Utah Research Foundation | Assay procedure using fluorogenic tracers |
DE4438660A1 (de) * | 1994-10-28 | 1996-05-02 | Boehringer Mannheim Gmbh | Biolumineszenzmarkierte Hapten-Konjugate für den Einsatz in kompetitiven Immunoassays |
BE1009196A3 (fr) * | 1995-03-09 | 1996-12-03 | Univ Catholique Louvain | Composition pharmaceutique, cosmetique et/ou alimentaire aux proprietes anti-oxydantes. |
US5661035A (en) * | 1995-06-07 | 1997-08-26 | The Regents Of The University Of California | Voltage sensing by fluorescence resonance energy transfer |
US6342379B1 (en) | 1995-06-07 | 2002-01-29 | The Regents Of The University Of California | Detection of transmembrane potentials by optical methods |
US6596522B2 (en) | 1997-05-08 | 2003-07-22 | The Regents Of The University Of California | Detection of transmembrane potentials by optical methods |
DE19537952A1 (de) | 1995-10-12 | 1997-04-17 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis eines Analyten |
US6124128A (en) * | 1996-08-16 | 2000-09-26 | The Regents Of The University Of California | Long wavelength engineered fluorescent proteins |
DE19652576A1 (de) * | 1996-12-17 | 1998-06-25 | Boehringer Mannheim Gmbh | Niedermolekulare Photoprotein-Konjugate |
US5876935A (en) * | 1997-01-08 | 1999-03-02 | Dade Behring Inc. | Luminescent specific binding assay |
EP1925320A3 (en) * | 1998-03-27 | 2008-09-03 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
WO2000014271A1 (en) * | 1998-09-03 | 2000-03-16 | Loma Linda University | METHOD FOR STUDYING PROTEIN INTERACTIONS $i(IN VIVO) |
WO2005050206A2 (en) * | 2003-09-17 | 2005-06-02 | Molecular Probes, Inc. | Competitive immunoassay |
US8329390B2 (en) | 2004-10-21 | 2012-12-11 | Anaspec Incorporated | Detection of transmembrane potentials using N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonols |
CN108802373A (zh) * | 2018-05-31 | 2018-11-13 | 湖南远璟生物技术有限公司 | 一种孕酮磁微粒化学发光免疫定量检测试剂盒及其制备方法 |
CN108802365A (zh) * | 2018-05-31 | 2018-11-13 | 湖南远璟生物技术有限公司 | 一种孕酮酶结合物的制备方法 |
CN109030811A (zh) * | 2018-05-31 | 2018-12-18 | 湖南远璟生物技术有限公司 | 一种睾酮酶结合物的制备方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1079045B (it) * | 1977-05-16 | 1985-05-08 | Syva Co | Analisi imuunologica fluorescente a doppio ricettore |
SE7811630L (sv) * | 1977-11-17 | 1979-05-18 | Welsh Nat School Med | Metod for uppteckt eller mengdbestemning av substanser med anvendning av merkningsteknik |
WO1981001883A1 (en) * | 1979-12-19 | 1981-07-09 | Electro Nucleonics | Chemical luminescence amplification substrate system for immunochemistry |
CA1179942A (en) * | 1981-10-06 | 1984-12-27 | Lubert Stryer | Fluorescent conjugates for analysis of molecules and cells |
-
1984
- 1984-10-11 DK DK487784A patent/DK487784A/da not_active Application Discontinuation
- 1984-10-11 ES ES536680A patent/ES8607563A1/es not_active Expired
- 1984-10-12 EP EP84112326A patent/EP0137515A3/en not_active Withdrawn
- 1984-10-12 FI FI844017A patent/FI844017L/fi not_active Application Discontinuation
- 1984-10-12 JP JP59214022A patent/JPS6122254A/ja active Pending
- 1984-10-12 CA CA000465285A patent/CA1234756A/en not_active Expired
- 1984-10-12 NO NO844102A patent/NO164442C/no unknown
- 1984-10-12 AU AU34203/84A patent/AU590511B2/en not_active Ceased
-
1985
- 1985-12-16 ES ES549950A patent/ES8705976A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ES8607563A1 (es) | 1986-06-01 |
CA1234756A (en) | 1988-04-05 |
ES549950A0 (es) | 1987-05-16 |
DK487784D0 (da) | 1984-10-11 |
NO844102L (no) | 1985-04-15 |
EP0137515A3 (en) | 1988-01-07 |
FI844017A0 (fi) | 1984-10-12 |
ES536680A0 (es) | 1986-06-01 |
FI844017L (fi) | 1985-04-14 |
EP0137515A2 (en) | 1985-04-17 |
AU590511B2 (en) | 1989-11-09 |
NO164442C (no) | 1990-10-03 |
NO164442B (no) | 1990-06-25 |
AU3420384A (en) | 1985-04-18 |
JPS6122254A (ja) | 1986-01-30 |
DK487784A (da) | 1985-04-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20041102 |