ES8609477A1 - Un metodo para aislar material genetico a partir de cepas delevaduras del genero pichia - Google Patents

Un metodo para aislar material genetico a partir de cepas delevaduras del genero pichia

Info

Publication number
ES8609477A1
ES8609477A1 ES548305A ES548305A ES8609477A1 ES 8609477 A1 ES8609477 A1 ES 8609477A1 ES 548305 A ES548305 A ES 548305A ES 548305 A ES548305 A ES 548305A ES 8609477 A1 ES8609477 A1 ES 8609477A1
Authority
ES
Spain
Prior art keywords
pichia
genes
phosphoribosyl
atp
yeast strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES548305A
Other languages
English (en)
Other versions
ES548305A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phillips Petroleum Co
Original Assignee
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phillips Petroleum Co filed Critical Phillips Petroleum Co
Publication of ES548305A0 publication Critical patent/ES548305A0/es
Publication of ES8609477A1 publication Critical patent/ES8609477A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

METODO DE AISLAMIENTO DE GENES FUNCIONALES DE CEPAS DE LEVADURA DEL GENERO PICHIA. SEGUN ESTE METODO SE AISLA Y CARACTERIZA EL GEN HIS4, DESCUBRIENDOSE, AISLANDOSE Y CARACTERIZANDOSE UNA REGION REGULADORA RESPONSABLE DE LA PRESENCIA O AUSENCIA DE AMINOACIDOS UTIL PARA LA EXPRESION CONTROLADA DE PRODUCTOS POLIPEPTIDICOS EN LEVADURAS. TRANSFORMACION INTEGRADORA DE LAS CEPAS DE LEVADURA DEL GENERO PICHIA, QUE PROPORCIONA UN MEDIO PARA RECOMBINACION DE SECUENCIAS DE VECTORES EN EL ADN CROMOSOMICO DEL HOSPEDANTE. ESTA RECOMBINACION DA COMO RESULTADO Y QUE SE MANTENGAN ESTABLES SECUENCIAS DE ADN INSERTADAS COMO ADN EXTRACROMOSOMICO. LOS GENES FUNCIONALES DE CEPAS DE LEVADURA DEL GENERO PICHIA SON UTILES COMO MARCADORES DE SELECCION GENOTIPICA.
ES548305A 1984-10-30 1985-10-29 Un metodo para aislar material genetico a partir de cepas delevaduras del genero pichia Expired ES8609477A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/666,578 US4885242A (en) 1984-10-30 1984-10-30 Genes from pichia histidine pathway and uses thereof

Publications (2)

Publication Number Publication Date
ES548305A0 ES548305A0 (es) 1986-07-16
ES8609477A1 true ES8609477A1 (es) 1986-07-16

Family

ID=24674601

Family Applications (1)

Application Number Title Priority Date Filing Date
ES548305A Expired ES8609477A1 (es) 1984-10-30 1985-10-29 Un metodo para aislar material genetico a partir de cepas delevaduras del genero pichia

Country Status (12)

Country Link
US (1) US4885242A (es)
EP (1) EP0188677A3 (es)
JP (3) JP2617443B2 (es)
AU (1) AU571748B2 (es)
DK (1) DK496285A (es)
ES (1) ES8609477A1 (es)
FI (1) FI854144L (es)
GR (1) GR852608B (es)
IL (1) IL76764A0 (es)
NO (1) NO854333L (es)
PT (1) PT81401B (es)
ZA (1) ZA858182B (es)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
CA2000101A1 (en) * 1988-12-22 1990-06-22 Mary E. Digan Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene
AU5196290A (en) * 1989-02-13 1990-09-05 Salk Institute Biotechnology/Industrial Associates, Inc., The Production of superoxide dismutase in pichia pastoris yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
DE69232666T2 (de) * 1991-04-01 2003-03-20 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US6090916A (en) 1994-10-18 2000-07-18 Corvas International, Inc. Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
JP2002512812A (ja) * 1998-04-24 2002-05-08 ヘキスト・マリオン・ルセル 抗真菌性物質のサッカロミセス・セレビシエ由来の必須遺伝子を用いるスクリーニング法
EP0972847A3 (en) * 1998-04-24 2000-02-09 Hoechst Marion Roussel Method for screening antimycotic substances
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
ES2330330T3 (es) * 2000-06-28 2009-12-09 Glycofi, Inc. Procedimiento de produccion de glucoproteinas modificadas.
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
DE60335602D1 (de) * 2002-12-18 2011-02-17 Roche Diagnostics Gmbh Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
DE60319333D1 (de) * 2002-12-20 2008-04-10 Roche Diagnostics Gmbh Hitzelabile Desoxyribonuklease I-Varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
ATE455849T1 (de) * 2003-12-05 2010-02-15 Hoffmann La Roche Rekombinante carboxypeptidase b und ihre aufreinigung
SI1926749T1 (sl) * 2005-09-14 2011-11-30 Sanofi Aventis Deutschland Cepitev prekurzorjev insulinov z varianto tripsina
DE202008014341U1 (de) 2008-10-28 2009-02-12 Heßler, Christoph, Dr. Mobile Fräse zur Kantenbearbeitung

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4615974A (en) * 1981-08-25 1986-10-07 Celltech Limited Yeast expression vectors
US4617274A (en) * 1981-10-29 1986-10-14 Phillips Petroleum Company Biochemical conversions by yeast fermentation at high cell densities
EP0128743A3 (en) * 1983-06-07 1985-12-27 Genex Corporation Expression of foreign genes in yeast
AU578486B2 (en) * 1984-08-03 1988-10-27 Ronald Norman Belsham Picture frame clamp
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia

Also Published As

Publication number Publication date
IL76764A0 (en) 1986-02-28
DK496285A (da) 1986-05-01
JPH07163354A (ja) 1995-06-27
AU571748B2 (en) 1988-04-21
FI854144A0 (fi) 1985-10-23
US4885242A (en) 1989-12-05
GR852608B (es) 1986-03-04
NO854333L (no) 1986-05-02
EP0188677A2 (en) 1986-07-30
JP2617443B2 (ja) 1997-06-04
ZA858182B (en) 1986-06-25
DK496285D0 (da) 1985-10-29
EP0188677A3 (en) 1987-09-30
PT81401B (pt) 1987-11-11
JP2710610B2 (ja) 1998-02-10
PT81401A (en) 1985-11-01
AU4875185A (en) 1986-06-12
JPS61108391A (ja) 1986-05-27
JPH09168388A (ja) 1997-06-30
ES548305A0 (es) 1986-07-16
FI854144L (fi) 1986-05-01

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