ES8605904A1 - Metodo inmunologico para la deteccion de celulas malignas con actividad metastatica - Google Patents

Metodo inmunologico para la deteccion de celulas malignas con actividad metastatica

Info

Publication number
ES8605904A1
ES8605904A1 ES532597A ES532597A ES8605904A1 ES 8605904 A1 ES8605904 A1 ES 8605904A1 ES 532597 A ES532597 A ES 532597A ES 532597 A ES532597 A ES 532597A ES 8605904 A1 ES8605904 A1 ES 8605904A1
Authority
ES
Spain
Prior art keywords
type
malignant cells
monoclonal antibodies
antibodies specific
collagenase enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES532597A
Other languages
English (en)
Other versions
ES532597A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of ES8605904A1 publication Critical patent/ES8605904A1/es
Publication of ES532597A0 publication Critical patent/ES532597A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C12N9/6491—Matrix metalloproteases [MMP's], e.g. interstitial collagenase (3.4.24.7); Stromelysins (3.4.24.17; 3.2.1.22); Matrilysin (3.4.24.23)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2474/00—Immunochemical assays or immunoassays characterised by detection mode or means of detection
    • G01N2474/20—Immunohistochemistry assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y10S436/813—Cancer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

METODO INMUNOLOGICO PARA LA DETECCION DE CELULAS MALIGNAS CON ACTIVIDAD METASTATICA. CONSISTE EN DETERMINAR ANTIGENOS ENZIMATICOS DE COLAGENASA A PARTIR DE MUESTRAS BIOLOGICAS O POR MEDIO DE INMUNOENSAYOS COMPETITIVOS UTILIZANDO ANTIGENOS ENZIMATICOS DE COLAGENASA TIPO IV Y/O ANTICUERPOS SEÑALADOS PARA DICHOS ANTIGENOS ENZIMATICOS, EN DONDE LOS ANTIGENOS O ANTICUERPOS PUEDEN LIGARSE A VEHICULOS SOLIDOS APROPIADOS O DESLIGARSE DE ESTOS, Y DONDE SE INCUBAN SECCIONES DE TEJIDOS O CELULAS CON UN PRIMER ANTICUERPO ESPECIFICO A DICHOS ANTIGENOS ENZIMATICOS DE COLAGENASA TIPO IV, Y DESPUES DE UN PERIODO DE TIEMPO SUFICIENTE PARA PERMITIR QUE LOS PRIMEROS ANTICUERPOS SE LIGUEN AL ANTIGENO ENZIMATICO, ADICIONAR UN SEGUNDO ANTICUERPO SEÑALADO A DICHO PRIMER ANTICUERPO QUE LIGA DICHO PRIMER ANTICUERPO, INDICANDO ASI LA PRESENCIA DE ANTIGENO ENZIMATICO DE COLAGENASA TIPO IV EN LA MUESTRA.
ES532597A 1983-05-19 1984-05-18 Metodo inmunologico para la deteccion de celulas malignas con actividad metastatica Expired ES8605904A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/496,230 US4677058A (en) 1983-05-19 1983-05-19 Detecting malignant cells with monoclonal antibodies specific to type IV collagenase enzyme

Publications (2)

Publication Number Publication Date
ES8605904A1 true ES8605904A1 (es) 1986-04-01
ES532597A0 ES532597A0 (es) 1986-04-01

Family

ID=23971775

Family Applications (1)

Application Number Title Priority Date Filing Date
ES532597A Expired ES8605904A1 (es) 1983-05-19 1984-05-18 Metodo inmunologico para la deteccion de celulas malignas con actividad metastatica

Country Status (26)

Country Link
US (1) US4677058A (es)
JP (1) JPS602187A (es)
AU (1) AU2808784A (es)
BE (1) BE899663A (es)
CA (1) CA1230052A (es)
CH (1) CH668838A5 (es)
DD (1) DD251208A5 (es)
DE (1) DE3418625A1 (es)
DK (1) DK243184A (es)
ES (1) ES8605904A1 (es)
FI (1) FI841897A (es)
FR (1) FR2546302B1 (es)
GB (1) GB2142030B (es)
HU (1) HU195004B (es)
IL (1) IL71802A0 (es)
IT (1) IT1175842B (es)
LU (1) LU85366A1 (es)
NL (1) NL8401616A (es)
NO (1) NO841993L (es)
NZ (1) NZ208108A (es)
PL (1) PL247745A1 (es)
PT (1) PT78610B (es)
RO (1) RO92427A (es)
SE (1) SE8402703L (es)
YU (1) YU86984A (es)
ZA (1) ZA843539B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6294172B1 (en) 1983-08-12 2001-09-25 Dade Behring Marburg Gmbh Monoclonal antibodies with specificity for membrane-associated antigens
US4859581A (en) * 1986-03-10 1989-08-22 Board Of Regents, The University Of Texas System Endoglycosidase assay
GB2209526B (en) * 1987-09-04 1992-06-03 Univ Washington Dna clone of human type iv collagenase
US4923818A (en) * 1987-09-04 1990-05-08 Washington University DNA clone of human type IV collagenase
US4876332A (en) * 1987-10-08 1989-10-24 Regents Of The Univeristy Of Minnesota Polypeptides with type IV collagen activity
US5280106A (en) * 1988-05-20 1994-01-18 Liotta Lance A Matrix metalloproteinase peptides: role in diagnosis and therapy
US5030555A (en) * 1988-09-12 1991-07-09 University Of Florida Membrane-strip reagent serodiagnostic apparatus and method
DE3909799A1 (de) * 1989-03-24 1990-09-27 Behringwerke Ag Monoklonale antikoerper (mak) gegen tumorassoziierte antigene, ihre herstellung und verwendung
US4992537A (en) * 1989-05-15 1991-02-12 Washington University cDNA encoding 92-kDA type IV collagenase
DK0495910T3 (da) 1989-10-18 2002-01-14 Us Health Fremstilling og anvendelse af humant nm23-H2-protein og derimod rettede antistoffer
CA2074507A1 (en) * 1990-01-26 1991-07-27 William G. Stetler-Stevenson Method for quantitatively measuring collagenase
WO1992020820A1 (en) * 1991-05-15 1992-11-26 Vanderbilt University Method to determine metastatic potential of tumor cells
US5324634A (en) * 1992-03-31 1994-06-28 The Research Foundation Of State University Of New York Diagnostic tests measuring gelatinase/inhibitor complexes for detection of aggressive and metastatic cancer
JP2673929B2 (ja) * 1993-04-12 1997-11-05 富士薬品工業株式会社 ヒト間質型コラゲナーゼと阻害剤との複合体の免疫学的定量法および臨床診断への応用
DE602005024418D1 (de) * 2004-08-26 2010-12-09 Life Technologies Corp Elektrobenetzende abgabevorrichtungen und dazugehörige verfahren

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE343949B (es) * 1966-06-02 1972-03-20 Pharmacia Ab
DE2363854C3 (de) * 1973-12-21 1980-01-03 Behringwerke Ag, 3550 Marburg Substrat zur Proteinasebestimmung sowie dessen Herstellung
US4201763A (en) * 1975-10-09 1980-05-06 Bio-Rad Laboratories, Inc. Solid phase immunofluorescent assay method
GB2109407B (en) * 1981-10-27 1985-12-18 Hybritech Inc Tumour imaging with radiolabelled monoclonal antibodies
US4424278A (en) * 1981-11-16 1984-01-03 Research Corporation Cancer detection procedure using an acyl carrier protein
US4443367A (en) * 1982-04-08 1984-04-17 Monsanto Company Peptide and peptolide substrates for mammalian collagenase

Also Published As

Publication number Publication date
PT78610A (en) 1984-06-01
LU85366A1 (fr) 1984-11-19
ZA843539B (en) 1985-07-31
BE899663A (fr) 1984-08-31
CA1230052A (en) 1987-12-08
US4677058A (en) 1987-06-30
ES532597A0 (es) 1986-04-01
GB8411959D0 (en) 1984-06-13
GB2142030B (en) 1987-02-04
GB2142030A (en) 1985-01-09
PT78610B (en) 1986-07-15
NO841993L (no) 1984-11-20
RO92427A (ro) 1987-09-30
DE3418625A1 (de) 1984-11-22
JPS602187A (ja) 1985-01-08
AU2808784A (en) 1984-11-22
NL8401616A (nl) 1984-12-17
DD251208A5 (de) 1987-11-04
IT1175842B (it) 1987-07-15
CH668838A5 (de) 1989-01-31
DK243184D0 (da) 1984-05-17
HU195004B (en) 1988-03-28
FR2546302A1 (fr) 1984-11-23
FI841897A (fi) 1984-11-20
FR2546302B1 (fr) 1988-03-04
HUT34261A (en) 1985-02-28
PL247745A1 (en) 1985-08-27
FI841897A0 (fi) 1984-05-11
SE8402703D0 (sv) 1984-05-18
IT8420998A1 (it) 1985-11-18
SE8402703L (sv) 1984-11-20
YU86984A (en) 1991-01-28
DK243184A (da) 1984-11-20
NZ208108A (en) 1988-04-29
IT8420998A0 (it) 1984-05-18
IL71802A0 (en) 1984-09-30

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