ES8602114A1 - Procedimiento para la obtencion de la nueva enzima 1-meti- lhidantoinasa. - Google Patents

Procedimiento para la obtencion de la nueva enzima 1-meti- lhidantoinasa.

Info

Publication number
ES8602114A1
ES8602114A1 ES540654A ES540654A ES8602114A1 ES 8602114 A1 ES8602114 A1 ES 8602114A1 ES 540654 A ES540654 A ES 540654A ES 540654 A ES540654 A ES 540654A ES 8602114 A1 ES8602114 A1 ES 8602114A1
Authority
ES
Spain
Prior art keywords
nucleosidetriphosphate
hydantoinase
dependent
methyl
methylhydan
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES540654A
Other languages
English (en)
Other versions
ES540654A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES540654A0 publication Critical patent/ES540654A0/es
Publication of ES8602114A1 publication Critical patent/ES8602114A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/70—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving creatine or creatinine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • G01N2333/986—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides (3.5.2), e.g. beta-lactamase (penicillinase, 3.5.2.6), creatinine amidohydrolase (creatininase, EC 3.5.2.10), N-methylhydantoinase (3.5.2.6)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Lubricants (AREA)
  • Detergent Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE ENZIMA L-METILHIDANTOINASA, CAPAZ DE HIDROLIZAR LA METILHIDANTOINA EN PRESENCIA DE UN NUCLEOSIDOTRIFOSFATO Y DE UN ION METALICO POLIVALENTE. CONSISTE EN EL CULTIVO DE ARTHROBACTER SPEC. DSM 2563, DSM 2564, MORAXELLA SPEC. DSM 2562, MICROCOCCUS SPEC. DSM 2565 O BREVIBACTERIUM SPEC. DSM 2843; SEGUIDA DE LA SEPARACION DE LA ENZIMA, PARA LO CUAL SE DISGREGA EL MICROORGANISMO, SE PRECIPITA LA ENZIMA POR ADICION DE POLIETILENIMINA Y EVENTUALMENTE SE ENRIQUECE MEDIANTE FRACCIONAMIENTO CON SULFATO AMONICO, UNA ETAPA DE CALENTAMIENTO A LA TEMPERATURA DE 50, EN UN MARGEN DE PH EN EL QUE LA ENZIMA TIENE BUENA ESTABILIDAD Y PURIFICACION POR CROMATOGRAFIA. ESTAS ENZIMAS TIENEN APLICACIONES EN QUIMICA ANALITICA, PARA EL DIAGNOSTICO CLINICO-QUIMICO.
ES540654A 1984-02-24 1985-02-22 Procedimiento para la obtencion de la nueva enzima 1-meti- lhidantoinasa. Expired ES8602114A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843406770 DE3406770A1 (de) 1984-02-24 1984-02-24 Nucleosidtriphosphat-abhaengige 1-methylhydantoinase und ihre verwendung

Publications (2)

Publication Number Publication Date
ES540654A0 ES540654A0 (es) 1985-12-01
ES8602114A1 true ES8602114A1 (es) 1985-12-01

Family

ID=6228749

Family Applications (1)

Application Number Title Priority Date Filing Date
ES540654A Expired ES8602114A1 (es) 1984-02-24 1985-02-22 Procedimiento para la obtencion de la nueva enzima 1-meti- lhidantoinasa.

Country Status (15)

Country Link
US (1) US4816393A (es)
EP (1) EP0154269B1 (es)
JP (1) JPS60203190A (es)
AT (1) ATE50789T1 (es)
AU (1) AU555270B2 (es)
CA (1) CA1261288A (es)
CZ (1) CZ277729B6 (es)
DD (1) DD241919A5 (es)
DE (2) DE3406770A1 (es)
DK (1) DK77185A (es)
ES (1) ES8602114A1 (es)
FI (1) FI82071C (es)
HU (1) HU194303B (es)
IE (1) IE58353B1 (es)
ZA (1) ZA851352B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4021571A1 (de) * 1990-07-06 1992-01-16 Boehringer Mannheim Gmbh N-methylhydantoinase, kloniert
DE4029844A1 (de) * 1990-09-20 1992-03-26 Boehringer Mannheim Gmbh Carbamoylsarcosinhydrolase, kloniert
DE4103220A1 (de) * 1991-02-02 1992-08-06 Boehringer Mannheim Gmbh Verfahren zur stabilisierung von 1-methylhydantoinase, verwendung einer stabilisierten 1-methylhydantoinase zur bestimmung eines analyten, entsprechendes bestimmungsverfahren sowie hierfuer geeignete mittel
US6524837B1 (en) 1999-03-29 2003-02-25 California Institute Of Technology Hydantoinase variants with improved properties and their use for the production of amino acids
EP1517996A4 (en) * 2002-05-01 2005-10-26 Polymer Technology Systems Inc TEST STRIP AND METHOD FOR DETERMINING THE CONCENTRATION OF CREATININE IN A BODILY FLUID
US7002670B2 (en) * 2002-06-12 2006-02-21 Baxter International Inc. Optical sensor and method for measuring concentration of a chemical constituent using its intrinsic optical absorbance
WO2009014722A1 (en) * 2007-07-25 2009-01-29 Richmond Chemical Corporation A method for detecting biofuel producing microbes
EP3435868A4 (en) * 2016-03-31 2020-01-01 Polymer Technology Systems, Inc. SYSTEMS AND METHODS FOR ELECTROCHEMICAL ASSAYS OF CREATININE

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2122294C3 (de) * 1971-05-05 1979-08-16 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Gewinnung von Creatininamidohydrolase
US4087329A (en) * 1975-08-28 1978-05-02 Kyowa Hakko Kogyo Co., Ltd. Creatinine desimidase and its method of production
US4134793A (en) * 1975-08-28 1979-01-16 Kyowa Hakko Kogyo Co., Ltd. Creatinine desimidase in the quantitative determination of creatinine
US4215197A (en) * 1978-08-04 1980-07-29 Miles Laboratories, Inc. Test means and method for creatinine determination
IT1137075B (it) * 1981-05-28 1986-09-03 Chemical Lab S R L Procedimento per la determinazione della creattinina (creatininio) in liquidi biologici, e reattivo per la sua attuazione
DE3248145A1 (de) * 1982-12-27 1984-06-28 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zur bestimmung von n-carbamoylsarcosin und hierfuer geeignetes neues enzym

Also Published As

Publication number Publication date
DK77185A (da) 1985-08-25
DK77185D0 (da) 1985-02-20
FI82071C (fi) 1991-01-10
EP0154269B1 (de) 1990-03-07
AU555270B2 (en) 1986-09-18
DD241919A5 (de) 1987-01-07
HUT37456A (en) 1985-12-28
JPS60203190A (ja) 1985-10-14
ZA851352B (en) 1985-10-30
EP0154269A2 (de) 1985-09-11
FI82071B (fi) 1990-09-28
IE850331L (en) 1985-08-24
DE3406770A1 (de) 1985-08-29
CZ277729B6 (en) 1993-04-14
CS121685A3 (en) 1992-06-17
JPH0373276B2 (es) 1991-11-21
CA1261288A (en) 1989-09-26
US4816393A (en) 1989-03-28
ES540654A0 (es) 1985-12-01
FI850732A0 (fi) 1985-02-22
IE58353B1 (en) 1993-09-08
FI850732L (fi) 1985-08-25
DE3576358D1 (de) 1990-04-12
AU3895485A (en) 1985-08-29
ATE50789T1 (de) 1990-03-15
HU194303B (en) 1988-01-28
EP0154269A3 (en) 1987-04-22

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