ES8507607A1 - Procedimiento para la fabricacion de la reductasa del acido 2, 5-diceto-d-gluconico - Google Patents
Procedimiento para la fabricacion de la reductasa del acido 2, 5-diceto-d-gluconicoInfo
- Publication number
- ES8507607A1 ES8507607A1 ES537480A ES537480A ES8507607A1 ES 8507607 A1 ES8507607 A1 ES 8507607A1 ES 537480 A ES537480 A ES 537480A ES 537480 A ES537480 A ES 537480A ES 8507607 A1 ES8507607 A1 ES 8507607A1
- Authority
- ES
- Spain
- Prior art keywords
- diketo
- gluconic acid
- acid reductase
- catalizes
- nadph
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- RXMWXENJQAINCC-DMTCNVIQSA-N 2,5-didehydro-D-gluconic acid Chemical compound OCC(=O)[C@@H](O)[C@H](O)C(=O)C(O)=O RXMWXENJQAINCC-DMTCNVIQSA-N 0.000 title abstract 2
- RXMWXENJQAINCC-UHFFFAOYSA-N 2,5-diketo-D-gluconic acid Natural products OCC(=O)C(O)C(O)C(=O)C(O)=O RXMWXENJQAINCC-UHFFFAOYSA-N 0.000 title abstract 2
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 abstract 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 2
- 150000003839 salts Chemical class 0.000 abstract 2
- VBUYCZFBVCCYFD-NUNKFHFFSA-N 2-dehydro-L-idonic acid Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)C(=O)C(O)=O VBUYCZFBVCCYFD-NUNKFHFFSA-N 0.000 abstract 1
- AWQIYVPBMVSGCL-PHDIDXHHSA-N 5-dehydro-D-fructose Chemical compound OCC(=O)[C@@H](O)[C@H](O)C(=O)CO AWQIYVPBMVSGCL-PHDIDXHHSA-N 0.000 abstract 1
- HDBDSFLMOWWRBQ-UHFFFAOYSA-N 5-fructonose Natural products OC1C(O)C2(O)COC1(O)CO2 HDBDSFLMOWWRBQ-UHFFFAOYSA-N 0.000 abstract 1
- 241000186216 Corynebacterium Species 0.000 abstract 1
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 abstract 1
- 239000003054 catalyst Substances 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
- C12P7/60—2-Ketogulonic acid
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/843—Corynebacterium
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PROCEDIMIENTO PARA LA FABRICACION DE LA REDUCTASA DEL ACIDO 2,5-DICETO-D-GLUCONICO.COMPRENDE: A) CULTIVAR UN MICROORGANISMO QUE TIENE UN GEN CORYNEBACTERIUM, EN UN MEDIO NUTRIENTE QUE CONTIENE FUENTES DE CARBONO COMO D-GLUCOSA Y SACAROSA, FUENTES DE NITROGENO COMO POLIPEPTONA Y SALES INORGANICAS COMO FOSFATOS, A UNA TEMPERATURA ENTRE 25J Y 35JC, DURANTE 15 A 30 H; Y B) AISLAR A PARTIR DE LAS CELULAS FRACTURADAS DE LOS MICROORGANISMOS, A LA REDUCTASA DEL ACIDO 2,5-DICETO-D-GLUCONICO QUE TIENE LAS SIGUIENTES PROPIEDADES QUIMICAS: A) PH OPTIMO, PH 6 A 7; 2) ESTABILIDAD PH, PH 5 A 7; 3) PESO MOLECULAR 29.000 G 2.000; 4) PUNTO ISOELECTRICO PH 4,4 G 0,3; 5) ESPECIFICIDADES DE SUSTRATOS, EL ACIDO 2,5-DICETO-D-GLUCONICO Y EL 5-CETI-D-FRUCTOSA; 6) REDUCCION DE LA 5-CETO-D-FRUCTOSA A L-SORBOSA; Y 7) REDUCCION DEL ACIDO 2,5-DICETO-D-GLUCONICO O SUS SALES A ACIDO 2-CETO-L-GLUCONICO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58217176A JPS60118186A (ja) | 1983-11-17 | 1983-11-17 | ２・５−ジケト−ｄ−グルコン酸リダクタ−ゼ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8507607A1 true ES8507607A1 (es) | 1985-09-16 |
| ES537480A0 ES537480A0 (es) | 1985-09-16 |
Family
ID=16700049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES537480A Granted ES537480A0 (es) | 1983-11-17 | 1984-11-07 | Procedimiento para la fabricacion de la reductasa del acido 2, 5-diceto-d-gluconico |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US4748122A (es) |
| EP (1) | EP0142169B1 (es) |
| JP (1) | JPS60118186A (es) |
| KR (1) | KR880001944B1 (es) |
| AU (1) | AU569925B2 (es) |
| CA (1) | CA1226836A (es) |
| DE (1) | DE3485321D1 (es) |
| DK (1) | DK545484A (es) |
| ES (1) | ES537480A0 (es) |
| GB (1) | GB2151233B (es) |
| HU (1) | HU192892B (es) |
| IE (1) | IE57594B1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3485981T2 (de) * | 1983-06-28 | 1993-06-09 | Genentech Inc | Biosynthetische 2,5-diketoglukonsaeure-reduktase, verfahren, rekombinante zellen und expressionsvektoren zu deren herstellung und ihre verwendung zur herstellung von 2-keto-l-gulonsaeure. |
| US5004690A (en) * | 1983-06-28 | 1991-04-02 | Genetech, Inc. | Ascorbic acid intermediates and process enzymes |
| US4755467A (en) * | 1985-06-03 | 1988-07-05 | Unisearch Limited | Method for the production of sorbitol and gluconate |
| AU594080B2 (en) * | 1985-08-02 | 1990-03-01 | Genencor International, Inc. | Production of a vitamin c precursor using genetically modified organisms |
| GB8519536D0 (en) * | 1985-08-02 | 1985-09-11 | Biogen Nv | Vitamin c precursor |
| US5032514A (en) * | 1988-08-08 | 1991-07-16 | Genentech, Inc. | Metabolic pathway engineering to increase production of ascorbic acid intermediates |
| US5376544A (en) * | 1992-09-08 | 1994-12-27 | Rutgers The State University Of New Jersey | Enzymes for the production of 2-keto-L-gulonic acid |
| US5795761A (en) * | 1996-01-11 | 1998-08-18 | Rutgers, The State University Of New Jersey | Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A |
| US7256027B1 (en) | 1999-06-15 | 2007-08-14 | Rutgers, The State University Of New Jersey | Enzymes for the production of 2-keto-L-gulonic acid |
| US6979717B2 (en) * | 2001-08-13 | 2005-12-27 | Moore Eugene R | Anionic process design for rapid polymerization of polystyrene without gel formation and product produced there from |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5021559B2 (es) * | 1973-03-22 | 1975-07-23 | ||
| JPS5135487A (en) * | 1974-09-20 | 1976-03-25 | Shionogi Seiyaku Kk | 22 keto ll guronsan no seizohoho |
| JPS5135485A (en) * | 1974-09-20 | 1976-03-25 | Shionogi Seiyaku Kk | 22 keto ll guronsan no seizohoho |
| JPS5135486A (en) * | 1974-09-20 | 1976-03-25 | Shionogi Seiyaku Kk | 22 keto ll guronsan no seizohoho |
| US4245049A (en) * | 1980-01-21 | 1981-01-13 | Pfizer Inc. | Preparation of 2-keto-L-gulonic acid |
| JPS58162298A (ja) * | 1982-03-05 | 1983-09-26 | Shionogi & Co Ltd | ２−ケト−ｌ−グロン酸の製造方法 |
| US4543331A (en) * | 1982-03-05 | 1985-09-24 | Shionogi & Co., Ltd. | Fermentative or enzymatic production of 2-keto-L-gulonic acid |
| DE3485981T2 (de) * | 1983-06-28 | 1993-06-09 | Genentech Inc | Biosynthetische 2,5-diketoglukonsaeure-reduktase, verfahren, rekombinante zellen und expressionsvektoren zu deren herstellung und ihre verwendung zur herstellung von 2-keto-l-gulonsaeure. |
-
1983
- 1983-11-17 JP JP58217176A patent/JPS60118186A/ja active Granted
-
1984
- 1984-10-24 CA CA000466243A patent/CA1226836A/en not_active Expired
- 1984-10-29 US US06/665,965 patent/US4748122A/en not_active Expired - Lifetime
- 1984-11-07 ES ES537480A patent/ES537480A0/es active Granted
- 1984-11-13 GB GB08428615A patent/GB2151233B/en not_active Expired
- 1984-11-13 IE IE2913/84A patent/IE57594B1/en not_active IP Right Cessation
- 1984-11-14 DE DE8484113750T patent/DE3485321D1/de not_active Expired - Fee Related
- 1984-11-14 EP EP84113750A patent/EP0142169B1/en not_active Expired - Lifetime
- 1984-11-16 HU HU844273A patent/HU192892B/hu not_active IP Right Cessation
- 1984-11-16 AU AU35612/84A patent/AU569925B2/en not_active Ceased
- 1984-11-16 DK DK545484A patent/DK545484A/da not_active Application Discontinuation
- 1984-11-16 KR KR1019840007209A patent/KR880001944B1/ko not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| KR880001944B1 (ko) | 1988-10-04 |
| US4748122A (en) | 1988-05-31 |
| CA1226836A (en) | 1987-09-15 |
| EP0142169A2 (en) | 1985-05-22 |
| GB8428615D0 (en) | 1984-12-19 |
| HU192892B (en) | 1987-07-28 |
| DE3485321D1 (de) | 1992-01-16 |
| IE842913L (en) | 1985-05-18 |
| KR850003731A (ko) | 1985-06-26 |
| JPS60118186A (ja) | 1985-06-25 |
| ES537480A0 (es) | 1985-09-16 |
| DK545484A (da) | 1985-05-18 |
| EP0142169B1 (en) | 1991-12-04 |
| AU3561284A (en) | 1985-05-23 |
| AU569925B2 (en) | 1988-02-25 |
| GB2151233B (en) | 1987-04-29 |
| JPH0335B2 (es) | 1991-01-07 |
| EP0142169A3 (en) | 1987-03-04 |
| IE57594B1 (en) | 1993-01-13 |
| GB2151233A (en) | 1985-07-17 |
| DK545484D0 (da) | 1984-11-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD1A | Patent lapsed |
Effective date: 20041102 |