ES8500995A1 - Un procedimiento para la preparacion de un vector de clonacion de adn recombinante. - Google Patents

Un procedimiento para la preparacion de un vector de clonacion de adn recombinante.

Info

Publication number
ES8500995A1
ES8500995A1 ES521468A ES521468A ES8500995A1 ES 8500995 A1 ES8500995 A1 ES 8500995A1 ES 521468 A ES521468 A ES 521468A ES 521468 A ES521468 A ES 521468A ES 8500995 A1 ES8500995 A1 ES 8500995A1
Authority
ES
Spain
Prior art keywords
antibiotic
cell
resistance
streptomyces
coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES521468A
Other languages
English (en)
Other versions
ES521468A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of ES521468A0 publication Critical patent/ES521468A0/es
Publication of ES8500995A1 publication Critical patent/ES8500995A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Saccharide Compounds (AREA)
  • Portable Nailing Machines And Staplers (AREA)

Abstract

PROCEDIMIENTO PARA LA PREPARACION DE UN VECTOR DE CLONACION DE ADN RECOMBINANTE.COMPRENDE: A) DIGERIR EL PLASMIDO SCP2 O EL SCP2 O UN PLASMIDO QUE LO CONTENGA CON UNA ENZIMA DE RESTRICCION; B) HACER REACCIONAR EL PLASMIDO DIGERIDO DE LA ETAPA ANTERIOR CON UNA SECUENCIA DE ADN PREVIAMENTE DIGERIDA CON UN ENZIMA DE RESTRICCION SIMILAR AL DE LA ETAPA (A), POR MEDIO DE UNA ADN LIGASA, CON EL FIN DE LIGAR DICHO PLASMIDO Y SECUENCIA DE ADN DIGERIDOS; Y C) TRANSFORMAR UNA CELULA CON EL PLASMIDO ASI OBTENIDO Y AISLAR EL PLASMIDO DE LAS CELULAS TRANSFORMADAS, CONSTITUIDO POR UN FRAGMENTO DE RESTRICCION DEL PLASMIDO SCP2 O SCP2 QUE CONTIENE UN ORIGEN FUNCIONAL DE REPLICACION Y UNO O MAS SECUENCIAS DE ADN.
ES521468A 1982-04-16 1983-04-14 Un procedimiento para la preparacion de un vector de clonacion de adn recombinante. Expired ES8500995A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36894782A 1982-04-16 1982-04-16

Publications (2)

Publication Number Publication Date
ES521468A0 ES521468A0 (es) 1984-11-01
ES8500995A1 true ES8500995A1 (es) 1984-11-01

Family

ID=23453416

Family Applications (1)

Application Number Title Priority Date Filing Date
ES521468A Expired ES8500995A1 (es) 1982-04-16 1983-04-14 Un procedimiento para la preparacion de un vector de clonacion de adn recombinante.

Country Status (19)

Country Link
JP (1) JPS58189198A (es)
KR (1) KR840004451A (es)
AT (1) ATE54327T1 (es)
AU (1) AU570631B2 (es)
CA (1) CA1207685A (es)
DD (1) DD211360A5 (es)
DE (1) DE3381699D1 (es)
DK (1) DK162083A (es)
ES (1) ES8500995A1 (es)
FI (1) FI831245L (es)
GB (1) GB2118947B (es)
GR (1) GR78559B (es)
IE (1) IE54818B1 (es)
IL (1) IL68349A0 (es)
NZ (1) NZ203864A (es)
PH (1) PH21843A (es)
PL (1) PL241511A1 (es)
PT (1) PT76536B (es)
ZA (1) ZA832526B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1203185A (en) * 1982-06-03 1986-04-15 Thomas G. Eckhardt Cloned streptomycete gene
US4468462A (en) * 1982-12-22 1984-08-28 Eli Lilly And Company Vectors for cloning in streptomyces
US4753886A (en) * 1984-08-10 1988-06-28 Eli Lilly And Company Plasmid PHJL210 and related bifunctional cloning vectors for use in streptomycetes
IL76421A0 (en) * 1984-09-27 1986-01-31 Lilly Co Eli Improvements in or relating to recombinant dna cosmid shuttle vectors
US4874748A (en) * 1986-03-24 1989-10-17 Abbott Laboratories Cloning vectors for streptomyces and use thereof in macrolide antibiotic production
US5149639A (en) * 1986-03-24 1992-09-22 Abbott Laboratories Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production
JP4726719B2 (ja) * 2006-03-31 2011-07-20 花王株式会社 抄紙シートの製造方法及び抄紙機
WO2013022504A1 (en) * 2011-05-06 2013-02-14 New England Biolabs, Inc. Ligation enhancement
CN114214356B (zh) * 2021-12-28 2023-11-24 塔里木大学 罗中链霉菌trm 49605遗传操作体系的构建方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3117131A1 (de) * 1981-04-30 1982-11-25 Hoechst Ag, 6000 Frankfurt "plasmid psg 2 und verfahren zu seiner gewinnung"
DE3128669A1 (de) * 1981-07-20 1983-02-03 Hoechst Ag, 6000 Frankfurt "plasmid p svh 1 und seine verwendung"
US4503155A (en) * 1982-02-01 1985-03-05 Eli Lilly And Company Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli

Also Published As

Publication number Publication date
IE830848L (en) 1983-10-16
AU570631B2 (en) 1988-03-24
DK162083D0 (da) 1983-04-13
PL241511A1 (en) 1983-12-05
DK162083A (da) 1983-10-17
CA1207685A (en) 1986-07-15
IE54818B1 (en) 1990-02-14
FI831245A0 (fi) 1983-04-13
PH21843A (en) 1988-03-17
GB2118947B (en) 1985-07-03
GB8310069D0 (en) 1983-05-18
ATE54327T1 (de) 1990-07-15
DD211360A5 (de) 1984-07-11
DE3381699D1 (de) 1990-08-09
PT76536B (en) 1986-03-12
ZA832526B (en) 1984-11-28
KR840004451A (ko) 1984-10-15
GB2118947A (en) 1983-11-09
GR78559B (es) 1984-09-27
ES521468A0 (es) 1984-11-01
IL68349A0 (en) 1983-07-31
JPS58189198A (ja) 1983-11-04
AU1347683A (en) 1983-10-20
FI831245L (fi) 1983-10-17
NZ203864A (en) 1986-08-08
PT76536A (en) 1983-05-01

Similar Documents

Publication Publication Date Title
EP0077650A3 (en) Cloning vectors
IE812297L (en) Stabilising transformed cells
IE822817L (en) Stabilization and selection of host cells containing¹recombinant dna
EP0077649A3 (en) Cloning vectors for use in Streptomyces
EP0085548A3 (en) Cloning vectors
ES8500995A1 (es) Un procedimiento para la preparacion de un vector de clonacion de adn recombinante.
WO1995002058A3 (en) Bacillus thuringiensis transposon tn5401 and its use in a site-specific recombination system for bacillus thuringiensis strain development
EP0115173A3 (en) Cloning vectors in streptomyces
DE3477936D1 (en) Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes
DK460183A (da) Funktionelt uafhaengige rekombinante dna-klonende vektorer til anvendelse i streptomyces
DK360785D0 (da) Plasmid phjl210 og dermed beslaegtede bifunktionelle kloningsvektorertil anvendelse i streptomyces
AU5971090A (en) Metalloproteinase inhibitor sequence recombinant vector system for using same and recombinant-dna method for the manufacture of same
DK0533701T3 (da) Færgevektor til udvikling af rekombinant Bacillus thuringiensis stamme
IL80559A0 (en) Streptomycin sensitive gene having many cloning sites,plasmid vector containing the same and microorganism containing the vector
AU551162B2 (en) Plasmid cloning vector for bacillus subtilis
Cohen et al. RNA polymerase from vegetative cells and spores of Bacillus cereus T: II. Peptide composition

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20040601