ES481782A1 - Un metodo de ensayo de fijacion especifico para determinar un ligando en un medio liquido. - Google Patents

Un metodo de ensayo de fijacion especifico para determinar un ligando en un medio liquido.

Info

Publication number
ES481782A1
ES481782A1 ES481782A ES481782A ES481782A1 ES 481782 A1 ES481782 A1 ES 481782A1 ES 481782 A ES481782 A ES 481782A ES 481782 A ES481782 A ES 481782A ES 481782 A1 ES481782 A1 ES 481782A1
Authority
ES
Spain
Prior art keywords
assay
prosthetic group
label component
residue
holoenzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES481782A
Other languages
English (en)
Inventor
William E Hornby
David L Morris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of ES481782A1 publication Critical patent/ES481782A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Prostheses (AREA)

Abstract

Ensayo de fijación específico mejorado por el empleo de un resto de grupo prostético orgánico como componente de marcado en el conjugado de marcado. Los grupos prostéticos son una clase de cofactores de enzimas reconocida en la técnica. Un cofactor de enzima es una sustancia no proteínica cuya presencia se requiere para que una enzima exhiba su actividad catalítica y que sufre un cambio químico durante el ciclo catalítico de la enzima de que se trate (a la que se hace referencia como la enzima madre). Una coenzima es un tipo de cofactor de enzima que se modifica químicamente en el curso de la reacción catalizada por la enzima madre. La regeneración de la forma original del cofactor requiere su participación en una reacción separada que es catalizada por una enzima diferente en la enzima madre.
ES481782A 1978-06-22 1979-06-21 Un metodo de ensayo de fijacion especifico para determinar un ligando en un medio liquido. Expired ES481782A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US91796178A 1978-06-22 1978-06-22

Publications (1)

Publication Number Publication Date
ES481782A1 true ES481782A1 (es) 1980-07-01

Family

ID=25439569

Family Applications (1)

Application Number Title Priority Date Filing Date
ES481782A Expired ES481782A1 (es) 1978-06-22 1979-06-21 Un metodo de ensayo de fijacion especifico para determinar un ligando en un medio liquido.

Country Status (21)

Country Link
JP (1) JPS6145776B2 (es)
AR (1) AR220947A1 (es)
AT (1) AT363615B (es)
AU (1) AU530673B2 (es)
BE (1) BE877060A (es)
BR (1) BR7903958A (es)
CH (1) CH659713A5 (es)
DD (1) DD144461A5 (es)
DE (1) DE2924249C2 (es)
DK (1) DK154670C (es)
ES (1) ES481782A1 (es)
FI (1) FI70726C (es)
FR (1) FR2429258B1 (es)
GB (1) GB2023607B (es)
IE (1) IE48264B1 (es)
IL (1) IL57570A (es)
LU (1) LU81410A1 (es)
NL (1) NL181527C (es)
NO (1) NO154447C (es)
SE (1) SE436522B (es)
ZA (1) ZA793068B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1183450A (en) * 1980-10-30 1985-03-05 Alfred C. Greenquist Homogeneous specific binding assay device and method
US4378428A (en) * 1981-03-30 1983-03-29 Baker Instruments Corporation Method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
AU574646B2 (en) * 1982-07-19 1988-07-14 Cooperbiomedical Inc. Enzyme assay method
US4550075A (en) * 1983-06-22 1985-10-29 Kallestad Laboratories, Inc. Method for ligand determination utilizing an immunoassay monitorable by biotin-containing enzymes, and compositions therefor
JPS60197698A (ja) * 1983-12-20 1985-10-07 カリフオルニア・インステイテユ−ト・オブ・テクノロジ− アミノ誘導化されたオリゴヌクレオチドの合成
GB8608435D0 (en) * 1986-04-07 1986-05-14 Cranfield Inst Of Tech Specific binding assays
EP0274343A1 (en) * 1987-01-06 1988-07-13 IntraCel Corporation A system of reactants involving an apo-enzyme useful in immunological analysis and a method for carrying out immunoassays with this system
NL8800820A (nl) * 1988-03-31 1989-10-16 Philips Nv Cassette.
KR101164048B1 (ko) * 2002-05-16 2012-07-18 에프. 호프만-라 로슈 아게 비-재생성 효소-보조효소 복합체를 포함하는 방법 및 시약시스템
EP1860439B1 (en) 2005-03-03 2010-10-13 Sekisui Medical Co., Ltd. Immunoassay method and reagent therefor
FR2889873B1 (fr) * 2005-08-18 2007-11-16 Ceva Sante Animale Sa Methode de detection de la progesterone dans le lait de vache et kit correspondant
EP3576129B1 (en) * 2018-06-01 2023-05-03 Thermo Fisher Scientific (Bremen) GmbH Method for detecting the isotopic labelling state of unknown species of molecules

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN142734B (es) * 1975-04-28 1977-08-20 Miles Lab

Also Published As

Publication number Publication date
NO792084L (no) 1979-12-28
AR220947A1 (es) 1980-12-15
IE791156L (en) 1979-12-22
SE7905514L (sv) 1979-12-23
NO154447B (no) 1986-06-09
GB2023607B (en) 1983-01-06
DK154670B (da) 1988-12-05
NO154447C (no) 1986-09-17
SE436522B (sv) 1984-12-17
FR2429258A1 (fr) 1980-01-18
AU4823079A (en) 1980-01-03
DD144461A5 (de) 1980-10-15
DK258079A (da) 1979-12-23
JPS552997A (en) 1980-01-10
NL181527C (nl) 1987-09-01
DE2924249C2 (de) 1989-04-27
AT363615B (de) 1981-08-25
BE877060A (fr) 1979-10-15
DE2924249A1 (de) 1980-01-03
LU81410A1 (fr) 1979-10-30
JPS6145776B2 (en) 1986-10-09
DK154670C (da) 1989-05-01
GB2023607A (en) 1980-01-03
ATA438979A (de) 1981-01-15
FR2429258B1 (fr) 1985-03-29
FI70726B (fi) 1986-06-26
CH659713A5 (de) 1987-02-13
IL57570A0 (en) 1979-10-31
AU530673B2 (en) 1983-07-28
FI70726C (fi) 1986-10-06
ZA793068B (en) 1980-08-27
BR7903958A (pt) 1980-02-12
IE48264B1 (en) 1984-11-14
NL7904879A (nl) 1979-12-28
IL57570A (en) 1982-07-30
FI791937A (fi) 1979-12-23

Similar Documents

Publication Publication Date Title
JPS5592698A (en) Test strip for glucose determination
ES481782A1 (es) Un metodo de ensayo de fijacion especifico para determinar un ligando en un medio liquido.
AU552420B2 (en) Unified test means for ion determination
GB1548741A (en) Heterogenous specific binding assay method and test systemfor use therein
IE45546L (en) Process for the determination of substrates or of enzyme¹activities
DK0470201T3 (da) Analysefremgangsmåde, reagenssammensætning og anvendelse deraf til glucosebestemmelse
ES495782A0 (es) Procedimiento de analisis para determinar la presencia de unanalito en una muestra liquida
JPS5663262A (en) Ascorbin acid resisting composition for detecting components in liquid sample testing device therefor and method thereof
SE7503358L (es)
ES467831A1 (es) Procedimiento para la preparacion de un reactivo para deter-minar un material inmunologicamente activo
ES8401136A1 (es) Un metodo enzimatico mejorado para el analisis cinetico de fluidos biologicos.
DE3777713D1 (de) Verfahren zum nachweis eines analyten sowie hierfuer geeignetes mittel.
GR3002496T3 (en) Immunometric assay
ES8302309A1 (es) Procedimiento para determinar la concentracion de glucosa en una muestra de ensayo.
DE3473475D1 (en) Process for determining substrate or enzymatic activity
AU2462388A (en) Method for self-performing enzyme kinetics
EP0278116A3 (en) A method for immunoassay and kit therefor
JPS63198999A (ja) リボフラビン連結検定法および材料
GB1535671A (en) Testing tube for determining the alcohol content of hydrocarbon fuels
JPS5682090A (en) Method and preparation of apo glucoseoxidase
JPS5636048A (en) Enzyme electrode
ES463068A1 (es) Metodo para la determinacion de peroxidos generados por reacciones enzimaticas yno quimicas.
JPS561896A (en) Determination of hydrogen peroxide and reagent for it
EP0255089A3 (en) Cobalt containing reagents and methods for the determination of analytes in aqueous fluids
NO168444C (no) Fremgangsmaate for fremstilling av en stabil, naturlig matriks til anvendelse ved immunologisk paavisning av psa

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20001009