ES479278A1 - Un metodo de ensayo para determinar la presencia en una muestra de un analito. - Google Patents

Un metodo de ensayo para determinar la presencia en una muestra de un analito.

Info

Publication number
ES479278A1
ES479278A1 ES479278A ES479278A ES479278A1 ES 479278 A1 ES479278 A1 ES 479278A1 ES 479278 A ES479278 A ES 479278A ES 479278 A ES479278 A ES 479278A ES 479278 A1 ES479278 A1 ES 479278A1
Authority
ES
Spain
Prior art keywords
environment control
specific receptor
label
receptor assays
phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES479278A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syva Co
Original Assignee
Syva Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/893,650 external-priority patent/US4233402A/en
Priority claimed from US05/964,099 external-priority patent/US4275149A/en
Application filed by Syva Co filed Critical Syva Co
Publication of ES479278A1 publication Critical patent/ES479278A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Un método de ensayo para determinar la presencia en una muestra de un analito que es un miembro de un par fijante específico que consta de un ligando y su receptor homólogo, empleando en dicho método (a) un medio acuoso continuo, (b) partículas sólidas dispersables discretas a las que está conjugado uno de los miembros de dicho par para dar un conjugado de partícula, definiendo dicho conjugado de partícula un medio ambiente alrededor de dicho miembro conjugado de dicho par diferente de dicho medio acuoso © un sistema productor de señal capaz de producir una señal medible, estando afectado el nivel de dicha señal por el grado en que dicho sistema productor de señal está dentro del medio ambiente de dichas partículas, (d) estando al menos un miembro de dicho sistema productor de señal conjugado a un miembro del para dar un conjugado de marcador de señal, en que la cantidad de conjugado de marcador de señal unido a dicho conjugado de partícula está relacionada con la cantidad de analito endicho medio acuoso, método que comprende: combinar en un medio acuoso de determinación (a) dicha muestra (b) dicho conjugado de partícula dispersado de modo sustancialmente uniforme en dicho medio © dicho conjugado de marcador de señal, (d) el miembro homólogo de dicho par fijante específico, siendo el analito, el conjugado de partícula y el conjugado de marcado de señal el mismo miembro del par fijante específico, y (e) cualquier miembro restante de dicho sistema productor de señal con la condición de que cuando el analito es un primer receptor, dicho miembro de dicho par conjugado a dicha partícula puede ser el ligando homólogo o receptor de dicho primer receptor, y dicho miembro de dicho conjugado de marcador de señal puede ser receptor para dicho primer receptor o el ligando homólogo respectivamente, y determinar el nivel de dicha señal en comparación con el medio de determinación que tiene una cantidad conocida de analito.
ES479278A 1978-04-05 1979-04-04 Un metodo de ensayo para determinar la presencia en una muestra de un analito. Expired ES479278A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US05/893,650 US4233402A (en) 1978-04-05 1978-04-05 Reagents and method employing channeling
US05/964,099 US4275149A (en) 1978-11-24 1978-11-24 Macromolecular environment control in specific receptor assays

Publications (1)

Publication Number Publication Date
ES479278A1 true ES479278A1 (es) 1979-12-01

Family

ID=27129059

Family Applications (1)

Application Number Title Priority Date Filing Date
ES479278A Expired ES479278A1 (es) 1978-04-05 1979-04-04 Un metodo de ensayo para determinar la presencia en una muestra de un analito.

Country Status (13)

Country Link
EP (1) EP0022768B1 (es)
JP (1) JPS54139794A (es)
AU (1) AU531777B2 (es)
CA (1) CA1140033A (es)
DE (2) DE2966245D1 (es)
ES (1) ES479278A1 (es)
FR (1) FR2435716B1 (es)
GB (1) GB2019562B (es)
IL (1) IL57042A (es)
IT (1) IT1198432B (es)
NL (1) NL7902663A (es)
SE (1) SE444086B (es)
WO (1) WO1979000882A1 (es)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
US4486530A (en) * 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
US4366241A (en) * 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
CA1183450A (en) * 1980-10-30 1985-03-05 Alfred C. Greenquist Homogeneous specific binding assay device and method
IL64574A (en) * 1981-04-27 1986-01-31 Syva Co Method for determining the presence of an analyte by determination of the amount of fluorescence in competitive protein binding assays
US4442218A (en) * 1981-05-27 1984-04-10 Corning Glass Works Method of measuring degree of partitioning
CA1209889A (en) * 1981-09-18 1986-08-19 Litai Weng Homogeneous assay on a non-porous surface
JPS5877843U (ja) * 1981-11-20 1983-05-26 株式会社三協精機製作所 テ−プレコ−ダ
JPS58209994A (ja) * 1982-05-10 1983-12-07 Fujirebio Inc 活性蛋白等固定化物を用いた抗原の測定方法
US4435504A (en) * 1982-07-15 1984-03-06 Syva Company Immunochromatographic assay with support having bound "MIP" and second enzyme
US4687735A (en) * 1983-03-14 1987-08-18 Syntex (U.S.A.) Inc. Enzymatic poly-reactant channeling binding assay
GB2136565B (en) * 1983-03-15 1986-05-08 Boots Celltech Diagnostics Heterogeneous binding assay
GB8307156D0 (en) * 1983-03-15 1983-04-20 Celltech Ltd Heterogeneous binding assays
US4550075A (en) * 1983-06-22 1985-10-29 Kallestad Laboratories, Inc. Method for ligand determination utilizing an immunoassay monitorable by biotin-containing enzymes, and compositions therefor
US4560648A (en) * 1983-09-23 1985-12-24 Syntex (U.S.A.) Inc. Homogeneous enzyme immunoassay for ferritin
US4578350A (en) * 1983-09-23 1986-03-25 Syntex (U.S.A.) Inc. Immunoassays employing protected labels
ZA849594B (en) * 1983-12-12 1985-08-28 Miles Lab Nucleic acid hybridization assay employing antibodies to intercalation complexes
ATE64622T1 (de) * 1984-04-23 1991-07-15 Boston Biomed Res Inst Doppelspezifische antikoerper-determinanten.
US4717654A (en) * 1984-06-19 1988-01-05 Akzo N.V. Process for solid phase platelet antibody assay
GB8423227D0 (en) * 1984-09-14 1984-10-17 Unilever Plc Materials for specific binding assays
FR2585836B1 (fr) * 1985-08-02 1987-11-27 Commissariat Energie Atomique Procede homogene de detection et/ou de determination par luminescence d'un analyte dans un milieu susceptible de le contenir
DE3541033A1 (de) * 1985-11-19 1987-05-21 Boehringer Mannheim Gmbh Verfahren zur quantifizierung von zellpopulationen bzw. subpopulationen sowie hierfuer geeignetes reagenz
JPH01500059A (ja) * 1986-05-22 1989-01-12 ユニリーバー・ナームローゼ・ベンノートシヤープ アッセイ
IL85597A (en) * 1987-05-18 1992-08-18 Technicon Instr Immunoassay method and composition using conjugate of analyte and reaction intermediate for analyte capable of mediating signal generation
US4943525A (en) * 1987-11-02 1990-07-24 Bioventures, Inc. Simultaneous immunoassay for the determination of antigens and antibodies
JP2607102B2 (ja) * 1987-11-07 1997-05-07 株式会社シノテスト 均一系による特異的結合部位を有する物質の測定法
CA2019711A1 (en) * 1989-06-28 1990-12-28 Takashi Ikegami Sensitization of assays
GB2236852B (en) * 1989-09-25 1994-04-06 Scotgen Ltd DNA probe based assays and intermediates useful in the synthesis of cleavable nucleic acids for use in such assays
JPH0534345A (ja) * 1991-02-19 1993-02-09 Tdk Corp 化学発光を利用する抗原・抗体の測定方法
JP2683172B2 (ja) * 1991-10-01 1997-11-26 キヤノン株式会社 検体測定方法及び検体測定装置
USRE38525E1 (en) * 1998-07-03 2004-06-08 Torsana Diabetes Diagnostics A/S Optical sensor for in situ measurement of analytes
GB9814506D0 (en) * 1998-07-03 1998-09-02 Stanley Christopher J Optical sensor for insitu measurement of analytes
EP1542014B1 (en) 1998-07-03 2006-10-18 Precisense A/S Optical sensor for in situ measurement of analytes

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3169956A (en) * 1962-04-17 1965-02-16 Ferrania Spa Sucrose organo-phosphorus ester and method of preparing it
US3652761A (en) * 1969-09-04 1972-03-28 Corning Glass Works Immunochemical composites and antigen or antibody purification therewith
NL154599B (nl) * 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3690834A (en) * 1971-01-11 1972-09-12 Syva Co Ligand determination with spin labeled compounds by receptor displacement
US4118349A (en) * 1971-05-12 1978-10-03 Behringwerke Aktiengesellschaft Process for the manufacture of polystyrene latex compounds
US3817837A (en) * 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3852157A (en) * 1971-05-14 1974-12-03 Syva Corp Compounds for enzyme amplification assay
US3853987A (en) * 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3826613A (en) * 1973-03-06 1974-07-30 Us Navy Detection and titration of viruses and antibodies using latex
JPS50894A (es) * 1973-04-28 1975-01-07
DE2440367C2 (de) * 1973-08-30 1984-03-15 General Electric Co., Schenectady, N.Y. Verfahren zum Herstellen multimolekularer Schichten aus immunologisch komplexierten Proteinen auf einem Substrat
US3951748A (en) * 1974-11-11 1976-04-20 Medical Products, Inc. Sensitized matrix for detection of disease
SE388694B (sv) * 1975-01-27 1976-10-11 Kabi Ab Sett att pavisa ett antigen exv i prov av kroppvetskor, med utnyttjande av till porost berarmaterial bundna eller adsorberande antikroppar
IN142734B (es) * 1975-04-28 1977-08-20 Miles Lab
US4181650A (en) * 1975-08-25 1980-01-01 Maier Charles L Jr Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids
FR2334106A1 (fr) * 1975-12-02 1977-07-01 Pasteur Institut Gel magnetique convenant pour dosages immunoenzymatiques
US4080264A (en) * 1976-03-01 1978-03-21 Massachusetts Institute Of Technology Immunoassay by light scattering spectroscopy
GB1572220A (en) * 1976-10-07 1980-07-30 Mochida Pharm Co Ltd Immunochemical process of measuring physiologically active substances
US4134792A (en) * 1976-12-06 1979-01-16 Miles Laboratories, Inc. Specific binding assay with an enzyme modulator as a labeling substance
US4130462A (en) * 1976-12-16 1978-12-19 Syva Company Receptor steric hindrance immunoassay for receptor determination

Also Published As

Publication number Publication date
FR2435716A1 (fr) 1980-04-04
IL57042A0 (en) 1979-07-25
EP0022768B1 (en) 1983-10-05
NL7902663A (nl) 1979-10-09
SE444086B (sv) 1986-03-17
GB2019562B (en) 1983-03-30
DE2966245D1 (en) 1983-11-10
GB2019562A (en) 1979-10-31
IT7948641A0 (it) 1979-04-05
SE7910042L (sv) 1979-12-05
CA1140033A (en) 1983-01-25
EP0022768A1 (en) 1981-01-28
JPH0114541B2 (es) 1989-03-13
WO1979000882A1 (en) 1979-11-01
FR2435716B1 (fr) 1986-02-21
IT1198432B (it) 1988-12-21
DE2913551A1 (de) 1979-10-25
JPS54139794A (en) 1979-10-30
EP0022768A4 (en) 1980-08-18
AU4562979A (en) 1979-10-11
AU531777B2 (en) 1983-09-08
IL57042A (en) 1982-07-30

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