ES465871A1 - Mejoras en un metodo colorimetrico para la determinacion de la actividad de la enzima -glutamil-transpeptidasa - Google Patents

Mejoras en un metodo colorimetrico para la determinacion de la actividad de la enzima -glutamil-transpeptidasa

Info

Publication number
ES465871A1
ES465871A1 ES465871A ES465871A ES465871A1 ES 465871 A1 ES465871 A1 ES 465871A1 ES 465871 A ES465871 A ES 465871A ES 465871 A ES465871 A ES 465871A ES 465871 A1 ES465871 A1 ES 465871A1
Authority
ES
Spain
Prior art keywords
gamma
glutamyl
colorimetric method
glutamyl transpeptidase
compositions useful
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES465871A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Electronics Inc
Original Assignee
Coulter Electronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc
Publication of ES465871A1 publication Critical patent/ES465871A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Mejoras en un método colorimétrico para la determinación de la actividad de la enzima gamma-glutamil-transpeptidasa en un fluido biológico, donde la enzima de dicho fluido se hace reaccionar catalíticamente con un substrato de un péptido sintético de ácido L-glutámico en un medio acuoso para formar un producto de escisión diazotable del péptido y el producto de escisión se diazota y se hace reaccionar con un compuesto copulante para producir un colorante con una intensidad de color proporcional a la actividad enzimática, cuya mejora consiste en emplear como substrato una sal soluble en agua de un compuesto seleccionado entre el grupo formado por L-gamma-glutamil-3-sulfoanilida y L-gamma-glutamil-4-sulfoanilida.
ES465871A 1977-01-12 1978-01-10 Mejoras en un metodo colorimetrico para la determinacion de la actividad de la enzima -glutamil-transpeptidasa Expired ES465871A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05/758,800 US4087331A (en) 1977-01-12 1977-01-12 Colorimetric method for determining gamma-glutamyl transpeptidase and compositions useful therein

Publications (1)

Publication Number Publication Date
ES465871A1 true ES465871A1 (es) 1979-01-01

Family

ID=25053170

Family Applications (1)

Application Number Title Priority Date Filing Date
ES465871A Expired ES465871A1 (es) 1977-01-12 1978-01-10 Mejoras en un metodo colorimetrico para la determinacion de la actividad de la enzima -glutamil-transpeptidasa

Country Status (12)

Country Link
US (1) US4087331A (es)
JP (1) JPS53111793A (es)
BE (1) BE862746A (es)
BR (1) BR7800102A (es)
DE (1) DE2801070A1 (es)
DK (1) DK11678A (es)
ES (1) ES465871A1 (es)
FR (1) FR2377631A1 (es)
GB (1) GB1596461A (es)
IT (1) IT1103114B (es)
NL (1) NL7800164A (es)
SE (1) SE7800201L (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS53147034A (en) * 1977-05-30 1978-12-21 Wako Pure Chem Ind Ltd Gamma-glutamyl-p-aminoanilide derivatives and process for their preparation
DE3070194D1 (en) * 1979-04-16 1985-03-28 Massachusetts Inst Technology An in vitro method for determining malignancy in mammalian tissue
US4334017A (en) * 1979-04-16 1982-06-08 Massachusetts Institute Of Technology Method for detecting cancer in mammalian tissue
JPS589698A (ja) * 1981-07-10 1983-01-20 Toyo Jozo Co Ltd γ−グルタミルトランスペプチダ−ゼ活性測定用基質、およびそれを用いる活性測定法
WO1983003254A1 (en) * 1982-03-11 1983-09-29 Arthur Babson Stabilization of diazonium salt solutions
JPS58198756A (ja) * 1982-05-14 1983-11-18 Nitto Boseki Co Ltd 新規な５−アミノサリチル酸の定量法
US4511651A (en) * 1982-07-30 1985-04-16 American Monitor Corporation Reagent composition and assay for the determination of γ-glutamyltransferase activity
US4588836A (en) * 1982-09-01 1986-05-13 Toyo Jozo Kabushiki Kaisha Novel synthetic substrate and assay method using the same
US4560650A (en) * 1983-06-08 1985-12-24 Coulter Electronics, Inc. Method and compositions for determination of gamma glutamyl transpeptidase
JPS60164499A (ja) * 1984-02-07 1985-08-27 Kyowa Medetsukusu Kk 酵素活性測定法
DE3687928T2 (de) * 1985-06-20 1993-07-08 Fuji Photo Film Co Ltd Reagenzblatt und integrierendes mehrschichtiges analytisches element zur messung der wirksamkeit von gamma-glutamyl-transferase.
US4751178A (en) * 1985-09-26 1988-06-14 Eastman Kodak Company Substrates, compositions, elements and methods for the determination of gamma-glutamyltransferase
US6280965B1 (en) 1999-05-26 2001-08-28 Nova Southeastern University Simple non-radioactive assay for estimating protein kinase C and protein phosphatase-1
WO2010038895A1 (ja) 2008-10-03 2010-04-08 味の素株式会社 ＣａＳＲアゴニスト
KR101493717B1 (ko) * 2010-03-04 2015-02-16 아지노모토 가부시키가이샤 알킬아민 유도체
EP2551262A4 (en) 2010-03-24 2014-01-15 Ajinomoto Kk GLUTAMATE DERIVATIVES OR SALTS THEREOF
CN114487409B (zh) * 2022-04-14 2022-07-19 启德医药科技（苏州）有限公司 一种转肽酶活性的检测方法及检测试剂盒

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3892631A (en) * 1972-08-21 1975-07-01 Warner Lambert Co Diagnostic reagents used for the determination of gamma-glutamyl transpeptidase in biological fluids
US3769173A (en) * 1972-08-21 1973-10-30 Warner Lambert Co Determination of gamma-glutamyl transpeptidase in biological fluids
US3986931A (en) * 1972-12-05 1976-10-19 Boehringer Mannheim G.M.B.H. γ-GLUTAMYL-4-NITROANILIDE COMPOUNDS AND THEIR USE IN DETERMINING γ-GLUTAMYL TRANSPEPTIDASE

Also Published As

Publication number Publication date
NL7800164A (nl) 1978-07-14
BR7800102A (pt) 1978-12-19
DE2801070A1 (de) 1978-07-20
SE7800201L (sv) 1978-07-13
IT7847597A0 (it) 1978-01-11
FR2377631A1 (fr) 1978-08-11
GB1596461A (en) 1981-08-26
US4087331A (en) 1978-05-02
IT1103114B (it) 1985-10-14
BE862746A (nl) 1978-05-02
DK11678A (da) 1978-07-13
JPS53111793A (en) 1978-09-29

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