ES465871A1 - Mejoras en un metodo colorimetrico para la determinacion de la actividad de la enzima -glutamil-transpeptidasa - Google Patents
Mejoras en un metodo colorimetrico para la determinacion de la actividad de la enzima -glutamil-transpeptidasaInfo
- Publication number
- ES465871A1 ES465871A1 ES465871A ES465871A ES465871A1 ES 465871 A1 ES465871 A1 ES 465871A1 ES 465871 A ES465871 A ES 465871A ES 465871 A ES465871 A ES 465871A ES 465871 A1 ES465871 A1 ES 465871A1
- Authority
- ES
- Spain
- Prior art keywords
- gamma
- glutamyl
- colorimetric method
- glutamyl transpeptidase
- compositions useful
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 101710107035 Gamma-glutamyltranspeptidase Proteins 0.000 title abstract 2
- 101710173228 Glutathione hydrolase proenzyme Proteins 0.000 title abstract 2
- 238000004737 colorimetric analysis Methods 0.000 title abstract 2
- 102000006640 gamma-Glutamyltransferase Human genes 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 230000008878 coupling Effects 0.000 abstract 2
- 238000010168 coupling process Methods 0.000 abstract 2
- 238000005859 coupling reaction Methods 0.000 abstract 2
- 230000007017 scission Effects 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- BEHLMOQXOSLGHN-UHFFFAOYSA-N benzenamine sulfate Chemical class OS(=O)(=O)NC1=CC=CC=C1 BEHLMOQXOSLGHN-UHFFFAOYSA-N 0.000 abstract 1
- 239000013060 biological fluid Substances 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Mejoras en un método colorimétrico para la determinación de la actividad de la enzima gamma-glutamil-transpeptidasa en un fluido biológico, donde la enzima de dicho fluido se hace reaccionar catalíticamente con un substrato de un péptido sintético de ácido L-glutámico en un medio acuoso para formar un producto de escisión diazotable del péptido y el producto de escisión se diazota y se hace reaccionar con un compuesto copulante para producir un colorante con una intensidad de color proporcional a la actividad enzimática, cuya mejora consiste en emplear como substrato una sal soluble en agua de un compuesto seleccionado entre el grupo formado por L-gamma-glutamil-3-sulfoanilida y L-gamma-glutamil-4-sulfoanilida.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/758,800 US4087331A (en) | 1977-01-12 | 1977-01-12 | Colorimetric method for determining gamma-glutamyl transpeptidase and compositions useful therein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES465871A1 true ES465871A1 (es) | 1979-01-01 |
Family
ID=25053170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES465871A Expired ES465871A1 (es) | 1977-01-12 | 1978-01-10 | Mejoras en un metodo colorimetrico para la determinacion de la actividad de la enzima -glutamil-transpeptidasa |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US4087331A (es) |
| JP (1) | JPS53111793A (es) |
| BE (1) | BE862746A (es) |
| BR (1) | BR7800102A (es) |
| DE (1) | DE2801070A1 (es) |
| DK (1) | DK11678A (es) |
| ES (1) | ES465871A1 (es) |
| FR (1) | FR2377631A1 (es) |
| GB (1) | GB1596461A (es) |
| IT (1) | IT1103114B (es) |
| NL (1) | NL7800164A (es) |
| SE (1) | SE7800201L (es) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS53147034A (en) * | 1977-05-30 | 1978-12-21 | Wako Pure Chem Ind Ltd | Gamma-glutamyl-p-aminoanilide derivatives and process for their preparation |
| DE3070194D1 (en) * | 1979-04-16 | 1985-03-28 | Massachusetts Inst Technology | An in vitro method for determining malignancy in mammalian tissue |
| US4334017A (en) * | 1979-04-16 | 1982-06-08 | Massachusetts Institute Of Technology | Method for detecting cancer in mammalian tissue |
| US4425427A (en) | 1980-03-13 | 1984-01-10 | Vitafin N.V. | Simultaneous, kinetic, spectrophotometric analysis of blood serum for multiple components |
| JPS589698A (ja) * | 1981-07-10 | 1983-01-20 | Toyo Jozo Co Ltd | γ−グルタミルトランスペプチダ−ゼ活性測定用基質、およびそれを用いる活性測定法 |
| EP0103628A4 (en) * | 1982-03-11 | 1984-09-19 | Arthur Babson | STABILIZATION OF DIAZONIUM SALINE SOLUTIONS. |
| JPS58198756A (ja) * | 1982-05-14 | 1983-11-18 | Nitto Boseki Co Ltd | 新規な５−アミノサリチル酸の定量法 |
| US4511651A (en) * | 1982-07-30 | 1985-04-16 | American Monitor Corporation | Reagent composition and assay for the determination of γ-glutamyltransferase activity |
| US4588836A (en) * | 1982-09-01 | 1986-05-13 | Toyo Jozo Kabushiki Kaisha | Novel synthetic substrate and assay method using the same |
| US4560650A (en) * | 1983-06-08 | 1985-12-24 | Coulter Electronics, Inc. | Method and compositions for determination of gamma glutamyl transpeptidase |
| JPS60164499A (ja) * | 1984-02-07 | 1985-08-27 | Kyowa Medetsukusu Kk | 酵素活性測定法 |
| US4916059A (en) * | 1985-06-20 | 1990-04-10 | Fuji Photo Film Co., Ltd. | Reagent sheet and integral multilayer analytical element for measurement of GGT activity |
| US4751178A (en) * | 1985-09-26 | 1988-06-14 | Eastman Kodak Company | Substrates, compositions, elements and methods for the determination of gamma-glutamyltransferase |
| US6280965B1 (en) | 1999-05-26 | 2001-08-28 | Nova Southeastern University | Simple non-radioactive assay for estimating protein kinase C and protein phosphatase-1 |
| WO2010038895A1 (ja) | 2008-10-03 | 2010-04-08 | 味の素株式会社 | ＣａＳＲアゴニスト |
| EP2546231B1 (en) * | 2010-03-04 | 2018-11-07 | EA Pharma Co., Ltd. | Alkylamine derivative |
| WO2011118843A1 (ja) | 2010-03-24 | 2011-09-29 | 味の素株式会社 | グルタミン酸エステル誘導体又はその塩 |
| CN114487409B (zh) * | 2022-04-14 | 2022-07-19 | 启德医药科技（苏州）有限公司 | 一种转肽酶活性的检测方法及检测试剂盒 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3892631A (en) * | 1972-08-21 | 1975-07-01 | Warner Lambert Co | Diagnostic reagents used for the determination of gamma-glutamyl transpeptidase in biological fluids |
| US3769173A (en) * | 1972-08-21 | 1973-10-30 | Warner Lambert Co | Determination of gamma-glutamyl transpeptidase in biological fluids |
| US3986931A (en) * | 1972-12-05 | 1976-10-19 | Boehringer Mannheim G.M.B.H. | γ-GLUTAMYL-4-NITROANILIDE COMPOUNDS AND THEIR USE IN DETERMINING γ-GLUTAMYL TRANSPEPTIDASE |
-
1977
- 1977-01-12 US US05/758,800 patent/US4087331A/en not_active Expired - Lifetime
-
1978
- 1978-01-06 NL NL7800164A patent/NL7800164A/xx not_active Application Discontinuation
- 1978-01-09 BR BR7800102A patent/BR7800102A/pt unknown
- 1978-01-09 BE BE184101A patent/BE862746A/xx unknown
- 1978-01-09 SE SE7800201A patent/SE7800201L/xx unknown
- 1978-01-10 DK DK11678A patent/DK11678A/da unknown
- 1978-01-10 ES ES465871A patent/ES465871A1/es not_active Expired
- 1978-01-11 IT IT47597/78A patent/IT1103114B/it active
- 1978-01-11 DE DE19782801070 patent/DE2801070A1/de not_active Withdrawn
- 1978-01-11 FR FR7800695A patent/FR2377631A1/fr active Pending
- 1978-01-12 GB GB1249/78A patent/GB1596461A/en not_active Expired
- 1978-01-12 JP JP158178A patent/JPS53111793A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US4087331A (en) | 1978-05-02 |
| IT1103114B (it) | 1985-10-14 |
| DE2801070A1 (de) | 1978-07-20 |
| SE7800201L (sv) | 1978-07-13 |
| FR2377631A1 (fr) | 1978-08-11 |
| JPS53111793A (en) | 1978-09-29 |
| NL7800164A (nl) | 1978-07-14 |
| BR7800102A (pt) | 1978-12-19 |
| IT7847597A0 (it) | 1978-01-11 |
| DK11678A (da) | 1978-07-13 |
| BE862746A (nl) | 1978-05-02 |
| GB1596461A (en) | 1981-08-26 |
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