ES346631A1 - Procedimiento de estabilizacion de reactivos enzimaticos deensayo combinados. - Google Patents
Procedimiento de estabilizacion de reactivos enzimaticos deensayo combinados.Info
- Publication number
- ES346631A1 ES346631A1 ES346631A ES346631A ES346631A1 ES 346631 A1 ES346631 A1 ES 346631A1 ES 346631 A ES346631 A ES 346631A ES 346631 A ES346631 A ES 346631A ES 346631 A1 ES346631 A1 ES 346631A1
- Authority
- ES
- Spain
- Prior art keywords
- dehydrogenase
- cysteine
- dinucleotide
- adenine
- compositions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000203 mixture Substances 0.000 title abstract 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 abstract 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 2
- 235000018417 cysteine Nutrition 0.000 abstract 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 abstract 2
- UPLLQJUZZIYKHI-DFWYDOINSA-N (2s)-2-aminopentanedioic acid;2-oxobutanedioic acid Chemical compound OC(=O)CC(=O)C(O)=O.OC(=O)[C@@H](N)CCC(O)=O UPLLQJUZZIYKHI-DFWYDOINSA-N 0.000 abstract 1
- OROGUZVNAFJPHA-UHFFFAOYSA-N 3-hydroxy-2,4-dimethyl-2H-thiophen-5-one Chemical compound CC1SC(=O)C(C)=C1O OROGUZVNAFJPHA-UHFFFAOYSA-N 0.000 abstract 1
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 abstract 1
- 102000004420 Creatine Kinase Human genes 0.000 abstract 1
- 108010042126 Creatine kinase Proteins 0.000 abstract 1
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 abstract 1
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 102000016901 Glutamate dehydrogenase Human genes 0.000 abstract 1
- 108010024636 Glutathione Proteins 0.000 abstract 1
- 108010063907 Glutathione Reductase Proteins 0.000 abstract 1
- 102100036442 Glutathione reductase, mitochondrial Human genes 0.000 abstract 1
- 108010009384 L-Iditol 2-Dehydrogenase Proteins 0.000 abstract 1
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 abstract 1
- FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 abstract 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 abstract 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 abstract 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 abstract 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 abstract 1
- NRFJZTXWLKPZAV-UHFFFAOYSA-N N-(2-oxo-3-thiolanyl)acetamide Chemical compound CC(=O)NC1CCSC1=O NRFJZTXWLKPZAV-UHFFFAOYSA-N 0.000 abstract 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract 1
- XJLXINKUBYWONI-NNYOXOHSSA-N NADP zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-N 0.000 abstract 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 abstract 1
- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 abstract 1
- 102000013009 Pyruvate Kinase Human genes 0.000 abstract 1
- 108020005115 Pyruvate Kinase Proteins 0.000 abstract 1
- 102100026974 Sorbitol dehydrogenase Human genes 0.000 abstract 1
- 108090000340 Transaminases Proteins 0.000 abstract 1
- 102000003929 Transaminases Human genes 0.000 abstract 1
- 229960004308 acetylcysteine Drugs 0.000 abstract 1
- 235000013877 carbamide Nutrition 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 229960004753 citiolone Drugs 0.000 abstract 1
- 239000005515 coenzyme Substances 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 229960003180 glutathione Drugs 0.000 abstract 1
- 235000003969 glutathione Nutrition 0.000 abstract 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 abstract 1
- -1 thioglycollates Chemical compound 0.000 abstract 1
- 239000001226 triphosphate Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Emergency Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Steering-Linkage Mechanisms And Four-Wheel Steering (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEB0090269 | 1966-12-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES346631A1 true ES346631A1 (es) | 1969-01-01 |
Family
ID=6985205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES346631A Expired ES346631A1 (es) | 1966-12-12 | 1967-10-31 | Procedimiento de estabilizacion de reactivos enzimaticos deensayo combinados. |
Country Status (11)
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3791929A (en) * | 1968-09-08 | 1974-02-12 | Searle & Co | Phosphatase assay using lyophilized aryl phosphate monesters |
| DE2050267C3 (de) * | 1970-10-13 | 1974-06-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur Herstellung von stabilen Zubereitungen von reduziertem Nicotinamidadenindinucleotid |
| BE786291A (fr) * | 1971-07-20 | 1973-01-15 | Technicon Instr | Compositions de diagnostic pour determination de la glutamate-oxalate-transaminase (got) et de la glutamate-pyruvate-transaminase (gpt) |
| DE3044454C2 (de) * | 1980-11-26 | 1982-12-09 | Boehringer Mannheim Gmbh, 6800 Mannheim | Stabilisierte Enzymzubereitung |
| EP0463755A1 (en) * | 1990-06-27 | 1992-01-02 | INSTRUMENTATION LABORATORY S.p.A. | Stable aqueous NADH reagent and kit |
| DE4124286A1 (de) * | 1991-07-22 | 1993-01-28 | Boehringer Mannheim Gmbh | Verfahren zur stabilisierung von proteinen bei optischen tests |
-
1966
- 1966-12-12 DE DE19661598157 patent/DE1598157C3/de not_active Expired
-
1967
- 1967-09-21 AT AT860767A patent/AT277937B/de not_active IP Right Cessation
- 1967-09-22 NO NO16982967A patent/NO122347B/no unknown
- 1967-09-26 GB GB4379467A patent/GB1153109A/en not_active Expired
- 1967-10-12 JP JP6569167A patent/JPS5018399B1/ja active Pending
- 1967-10-12 CH CH1424767A patent/CH481380A/de not_active IP Right Cessation
- 1967-10-17 DK DK515667A patent/DK133287C/da active
- 1967-10-26 NL NL6714582A patent/NL6714582A/xx not_active Application Discontinuation
- 1967-10-31 ES ES346631A patent/ES346631A1/es not_active Expired
- 1967-11-10 FI FI303467A patent/FI48511C/fi active
- 1967-12-12 BE BE707864D patent/BE707864A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NL6714582A (enrdf_load_stackoverflow) | 1968-06-13 |
| JPS5018399B1 (enrdf_load_stackoverflow) | 1975-06-28 |
| FI48511C (fi) | 1974-10-10 |
| DK133287B (da) | 1976-04-20 |
| DE1598157C3 (de) | 1973-11-22 |
| CH481380A (de) | 1969-11-15 |
| BE707864A (enrdf_load_stackoverflow) | 1968-06-12 |
| FI48511B (enrdf_load_stackoverflow) | 1974-07-01 |
| DE1598157B2 (de) | 1973-04-19 |
| DK133287C (da) | 1976-10-18 |
| GB1153109A (en) | 1969-05-21 |
| DE1598157A1 (de) | 1972-03-23 |
| NO122347B (enrdf_load_stackoverflow) | 1971-06-14 |
| AT277937B (de) | 1970-01-12 |
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