ES3052734T3 - Combined solution phase and solid phase dna amplification - Google Patents

Combined solution phase and solid phase dna amplification

Info

Publication number
ES3052734T3
ES3052734T3 ES25150312T ES25150312T ES3052734T3 ES 3052734 T3 ES3052734 T3 ES 3052734T3 ES 25150312 T ES25150312 T ES 25150312T ES 25150312 T ES25150312 T ES 25150312T ES 3052734 T3 ES3052734 T3 ES 3052734T3
Authority
ES
Spain
Prior art keywords
amplification
immobilized
primers
nucleic acid
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES25150312T
Other languages
English (en)
Spanish (es)
Inventor
David Allan Davidson
Graham Worsley
Jarrett Killpack
Samuel Reed
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dnae Diagnostics Ltd
Original Assignee
Dnae Diagnostics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dnae Diagnostics Ltd filed Critical Dnae Diagnostics Ltd
Application granted granted Critical
Publication of ES3052734T3 publication Critical patent/ES3052734T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6825Nucleic acid detection involving sensors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403Cells and electrode assemblies
    • G01N27/414Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/118Prognosis of disease development
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Electrochemistry (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES25150312T 2019-05-08 2020-05-07 Combined solution phase and solid phase dna amplification Active ES3052734T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB201906461 2019-05-08

Publications (1)

Publication Number Publication Date
ES3052734T3 true ES3052734T3 (en) 2026-01-13

Family

ID=67002052

Family Applications (3)

Application Number Title Priority Date Filing Date
ES25150312T Active ES3052734T3 (en) 2019-05-08 2020-05-07 Combined solution phase and solid phase dna amplification
ES23185680T Active ES3023484T3 (en) 2019-05-08 2020-05-07 Combined solution phase and solid phase dna amplification
ES20725896T Active ES2954784T3 (es) 2019-05-08 2020-05-07 Amplificación de ADN en fase de solución y en fase sólida combinadas

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES23185680T Active ES3023484T3 (en) 2019-05-08 2020-05-07 Combined solution phase and solid phase dna amplification
ES20725896T Active ES2954784T3 (es) 2019-05-08 2020-05-07 Amplificación de ADN en fase de solución y en fase sólida combinadas

Country Status (7)

Country Link
US (1) US20220333167A1 (enExample)
EP (3) EP3966346B1 (enExample)
JP (2) JP7611858B2 (enExample)
CN (3) CN114207146B (enExample)
CA (2) CA3139356C (enExample)
ES (3) ES3052734T3 (enExample)
WO (1) WO2020225564A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022104138A1 (en) * 2020-11-14 2022-05-19 Life Technologies Corporation System and method for automated repeat sequencing

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
JP2009000070A (ja) * 2007-06-22 2009-01-08 Hitachi Ltd 大規模並列核酸分析方法
JP4853403B2 (ja) * 2007-07-02 2012-01-11 セイコーエプソン株式会社 画像読み取り装置
WO2012042399A1 (en) * 2010-09-30 2012-04-05 Nxp B.V. Biosensor device and method
GB2500715A (en) * 2012-03-30 2013-10-02 Gene Onyx Ltd Single nucleotide polymorphism detection using an ISFET array
US9914968B2 (en) * 2012-09-26 2018-03-13 Cepheid Honeycomb tube
EP2722398B1 (en) * 2012-10-18 2017-07-12 F. Hoffmann-La Roche AG Dual probe assay for the detection of HCV
GB201407334D0 (en) * 2014-04-25 2014-06-11 Dna Electronics Ltd Integrated nucleic acid test system, instrument and method
EP3215631A4 (en) * 2014-11-05 2018-08-08 California Institute of Technology Microfluidic measurements of the response of an organism to a drug
WO2018046689A1 (en) * 2016-09-08 2018-03-15 Danmarks Tekniske Universitet A polymeric chip system and uses thereof

Also Published As

Publication number Publication date
CN119955908A (zh) 2025-05-09
EP4293124A3 (en) 2024-01-17
CA3139356A1 (en) 2020-11-12
EP4293124A2 (en) 2023-12-20
EP4524260B1 (en) 2025-10-22
CA3251083A1 (en) 2025-10-30
JP7611858B2 (ja) 2025-01-10
ES3023484T3 (en) 2025-06-02
CN114207146B (zh) 2025-01-10
EP3966346B1 (en) 2023-08-23
ES2954784T3 (es) 2023-11-24
JP2022531707A (ja) 2022-07-08
EP4524260A3 (en) 2025-05-14
CN119955908B (zh) 2026-05-08
US20220333167A1 (en) 2022-10-20
CA3139356C (en) 2025-04-01
JP2025060794A (ja) 2025-04-10
CN114207146A (zh) 2022-03-18
EP4524260A2 (en) 2025-03-19
CN119753103A (zh) 2025-04-04
EP3966346A1 (en) 2022-03-16
EP4293124B1 (en) 2025-02-19
WO2020225564A1 (en) 2020-11-12

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