CN114207146B - 组合的液相和固相dna扩增 - Google Patents

组合的液相和固相dna扩增 Download PDF

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Publication number
CN114207146B
CN114207146B CN202080034226.7A CN202080034226A CN114207146B CN 114207146 B CN114207146 B CN 114207146B CN 202080034226 A CN202080034226 A CN 202080034226A CN 114207146 B CN114207146 B CN 114207146B
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Prior art keywords
immobilized
nucleic acid
amplification
primer
primers
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Chinese (zh)
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CN114207146A (zh
Inventor
大卫·艾伦·戴维森
格雷厄姆·沃斯利
贾勒特·基尔帕克
赛缪尔·里德
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Dnae Diagnostics Ltd
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Dnae Diagnostics Ltd
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Priority to CN202510021594.2A priority Critical patent/CN119753103A/zh
Priority to CN202510020785.7A priority patent/CN119955908B/zh
Publication of CN114207146A publication Critical patent/CN114207146A/zh
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6825Nucleic acid detection involving sensors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403Cells and electrode assemblies
    • G01N27/414Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/118Prognosis of disease development
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Electrochemistry (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080034226.7A 2019-05-08 2020-05-07 组合的液相和固相dna扩增 Active CN114207146B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202510021594.2A CN119753103A (zh) 2019-05-08 2020-05-07 组合的液相和固相dna扩增
CN202510020785.7A CN119955908B (zh) 2019-05-08 2020-05-07 组合的液相和固相dna扩增

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201906461 2019-05-08
GB1906461.7 2019-05-08
PCT/GB2020/051122 WO2020225564A1 (en) 2019-05-08 2020-05-07 Combined solution phase and solid phase dna amplification

Related Child Applications (2)

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CN202510020785.7A Division CN119955908B (zh) 2019-05-08 2020-05-07 组合的液相和固相dna扩增
CN202510021594.2A Division CN119753103A (zh) 2019-05-08 2020-05-07 组合的液相和固相dna扩增

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CN114207146A CN114207146A (zh) 2022-03-18
CN114207146B true CN114207146B (zh) 2025-01-10

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CN202080034226.7A Active CN114207146B (zh) 2019-05-08 2020-05-07 组合的液相和固相dna扩增
CN202510021594.2A Pending CN119753103A (zh) 2019-05-08 2020-05-07 组合的液相和固相dna扩增
CN202510020785.7A Active CN119955908B (zh) 2019-05-08 2020-05-07 组合的液相和固相dna扩增

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CN202510020785.7A Active CN119955908B (zh) 2019-05-08 2020-05-07 组合的液相和固相dna扩增

Country Status (7)

Country Link
US (1) US20220333167A1 (enExample)
EP (3) EP3966346B1 (enExample)
JP (2) JP7611858B2 (enExample)
CN (3) CN114207146B (enExample)
CA (2) CA3139356C (enExample)
ES (3) ES3052734T3 (enExample)
WO (1) WO2020225564A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022104138A1 (en) * 2020-11-14 2022-05-19 Life Technologies Corporation System and method for automated repeat sequencing

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007086935A2 (en) * 2005-08-01 2007-08-02 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
JP2009000070A (ja) * 2007-06-22 2009-01-08 Hitachi Ltd 大規模並列核酸分析方法
JP4853403B2 (ja) * 2007-07-02 2012-01-11 セイコーエプソン株式会社 画像読み取り装置
WO2012042399A1 (en) * 2010-09-30 2012-04-05 Nxp B.V. Biosensor device and method
GB2500715A (en) * 2012-03-30 2013-10-02 Gene Onyx Ltd Single nucleotide polymorphism detection using an ISFET array
US9914968B2 (en) * 2012-09-26 2018-03-13 Cepheid Honeycomb tube
EP2722398B1 (en) * 2012-10-18 2017-07-12 F. Hoffmann-La Roche AG Dual probe assay for the detection of HCV
GB201407334D0 (en) * 2014-04-25 2014-06-11 Dna Electronics Ltd Integrated nucleic acid test system, instrument and method
EP3215631A4 (en) * 2014-11-05 2018-08-08 California Institute of Technology Microfluidic measurements of the response of an organism to a drug
WO2018046689A1 (en) * 2016-09-08 2018-03-15 Danmarks Tekniske Universitet A polymeric chip system and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007086935A2 (en) * 2005-08-01 2007-08-02 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids

Also Published As

Publication number Publication date
CN119955908A (zh) 2025-05-09
EP4293124A3 (en) 2024-01-17
CA3139356A1 (en) 2020-11-12
EP4293124A2 (en) 2023-12-20
EP4524260B1 (en) 2025-10-22
ES3052734T3 (en) 2026-01-13
CA3251083A1 (en) 2025-10-30
JP7611858B2 (ja) 2025-01-10
ES3023484T3 (en) 2025-06-02
EP3966346B1 (en) 2023-08-23
ES2954784T3 (es) 2023-11-24
JP2022531707A (ja) 2022-07-08
EP4524260A3 (en) 2025-05-14
CN119955908B (zh) 2026-05-08
US20220333167A1 (en) 2022-10-20
CA3139356C (en) 2025-04-01
JP2025060794A (ja) 2025-04-10
CN114207146A (zh) 2022-03-18
EP4524260A2 (en) 2025-03-19
CN119753103A (zh) 2025-04-04
EP3966346A1 (en) 2022-03-16
EP4293124B1 (en) 2025-02-19
WO2020225564A1 (en) 2020-11-12

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