ES3010648T3 - Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production - Google Patents

Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production Download PDF

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Publication number
ES3010648T3
ES3010648T3 ES21777795T ES21777795T ES3010648T3 ES 3010648 T3 ES3010648 T3 ES 3010648T3 ES 21777795 T ES21777795 T ES 21777795T ES 21777795 T ES21777795 T ES 21777795T ES 3010648 T3 ES3010648 T3 ES 3010648T3
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Prior art keywords
gene
cell line
endogenous
dhodh
sequence
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ES21777795T
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English (en)
Spanish (es)
Inventor
Bruno Louis Dumas
Mohammed Nabil Lounis
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Sanofi SA
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Sanofi SA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
    • C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Reproductive Health (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ES21777795T 2020-09-21 2021-09-21 Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production Active ES3010648T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20306068 2020-09-21
PCT/EP2021/075864 WO2022058603A1 (en) 2020-09-21 2021-09-21 Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production

Publications (1)

Publication Number Publication Date
ES3010648T3 true ES3010648T3 (en) 2025-04-04

Family

ID=72840426

Family Applications (1)

Application Number Title Priority Date Filing Date
ES21777795T Active ES3010648T3 (en) 2020-09-21 2021-09-21 Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production

Country Status (16)

Country Link
US (1) US20240043878A1 (https=)
EP (2) EP4214308B1 (https=)
JP (1) JP2023544267A (https=)
KR (1) KR20230068401A (https=)
CN (1) CN116457468A (https=)
AU (1) AU2021342718A1 (https=)
BR (1) BR112023005002A2 (https=)
CA (1) CA3195524A1 (https=)
ES (1) ES3010648T3 (https=)
HU (1) HUE070250T2 (https=)
IL (1) IL301442A (https=)
MX (1) MX2023003278A (https=)
PL (1) PL4214308T3 (https=)
TW (1) TWI912369B (https=)
WO (1) WO2022058603A1 (https=)
ZA (1) ZA202303597B (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12529071B2 (en) 2019-03-19 2026-01-20 Sanofi Selection marker-comprising cell line and uses thereof for protein production

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8717430D0 (en) 1987-07-23 1987-08-26 Celltech Ltd Recombinant dna product
KR100267720B1 (ko) 1997-12-29 2001-03-02 박호군 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법
US20030166201A1 (en) * 2001-04-30 2003-09-04 Jensen Michael C. Selection systems for genetically modified cells
JP5756016B2 (ja) * 2008-10-29 2015-07-29 サンガモ バイオサイエンシーズ， インコーポレイテッド グルタミンシンセターゼ遺伝子の発現を不活性化する方法及び組成物
EP2674495A1 (en) * 2012-06-14 2013-12-18 Sanofi CHO expression system
AU2015334872B2 (en) * 2014-10-23 2021-12-16 Sanofi Novel selection marker for cell transfection and protein production
US12497624B2 (en) * 2018-07-13 2025-12-16 Lonza Ltd Methods for improving production of biological products by reducing the level of endogenous protein
WO2020012446A1 (en) * 2018-07-13 2020-01-16 Enzene Biosciences Limited Double knock-out cho cell line method of its generation and producing therapeutic proteins therefrom
US12529071B2 (en) * 2019-03-19 2026-01-20 Sanofi Selection marker-comprising cell line and uses thereof for protein production
WO2020243642A2 (en) * 2019-05-31 2020-12-03 The Trustees Of Columbia University In The City Of New York Multiply auxotrophic cell line for the production of recombinant proteins and methods thereof
CN110343668B (zh) * 2019-07-23 2021-11-19 珠海联邦制药股份有限公司 一种敲除gs基因的cho细胞株及其制备方法与应用

Also Published As

Publication number Publication date
IL301442A (en) 2023-05-01
MX2023003278A (es) 2023-04-12
EP4214308B1 (en) 2024-11-20
US20240043878A1 (en) 2024-02-08
AU2021342718A9 (en) 2025-03-27
CN116457468A (zh) 2023-07-18
KR20230068401A (ko) 2023-05-17
EP4214308A1 (en) 2023-07-26
HUE070250T2 (hu) 2025-05-28
PL4214308T3 (pl) 2025-04-14
TWI912369B (zh) 2026-01-21
ZA202303597B (en) 2024-08-28
BR112023005002A2 (pt) 2023-04-18
WO2022058603A1 (en) 2022-03-24
AU2021342718A1 (en) 2023-06-08
TW202227622A (zh) 2022-07-16
JP2023544267A (ja) 2023-10-23
EP4541812A2 (en) 2025-04-23
CA3195524A1 (en) 2022-03-24
EP4541812A3 (en) 2025-06-11
EP4214308C0 (en) 2024-11-20

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