CA3195524A1 - Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production - Google Patents
Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein productionInfo
- Publication number
- CA3195524A1 CA3195524A1 CA3195524A CA3195524A CA3195524A1 CA 3195524 A1 CA3195524 A1 CA 3195524A1 CA 3195524 A CA3195524 A CA 3195524A CA 3195524 A CA3195524 A CA 3195524A CA 3195524 A1 CA3195524 A1 CA 3195524A1
- Authority
- CA
- Canada
- Prior art keywords
- gene
- dhodh
- cell line
- endogenous
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
- C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
- C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Reproductive Health (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20306068.6 | 2020-09-21 | ||
| EP20306068 | 2020-09-21 | ||
| PCT/EP2021/075864 WO2022058603A1 (en) | 2020-09-21 | 2021-09-21 | Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3195524A1 true CA3195524A1 (en) | 2022-03-24 |
Family
ID=72840426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3195524A Pending CA3195524A1 (en) | 2020-09-21 | 2021-09-21 | Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20240043878A1 (https=) |
| EP (2) | EP4214308B1 (https=) |
| JP (1) | JP2023544267A (https=) |
| KR (1) | KR20230068401A (https=) |
| CN (1) | CN116457468A (https=) |
| AU (1) | AU2021342718A1 (https=) |
| BR (1) | BR112023005002A2 (https=) |
| CA (1) | CA3195524A1 (https=) |
| ES (1) | ES3010648T3 (https=) |
| HU (1) | HUE070250T2 (https=) |
| IL (1) | IL301442A (https=) |
| MX (1) | MX2023003278A (https=) |
| PL (1) | PL4214308T3 (https=) |
| TW (1) | TWI912369B (https=) |
| WO (1) | WO2022058603A1 (https=) |
| ZA (1) | ZA202303597B (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12529071B2 (en) | 2019-03-19 | 2026-01-20 | Sanofi | Selection marker-comprising cell line and uses thereof for protein production |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
| KR100267720B1 (ko) | 1997-12-29 | 2001-03-02 | 박호군 | 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법 |
| US20030166201A1 (en) * | 2001-04-30 | 2003-09-04 | Jensen Michael C. | Selection systems for genetically modified cells |
| JP5756016B2 (ja) * | 2008-10-29 | 2015-07-29 | サンガモ バイオサイエンシーズ， インコーポレイテッド | グルタミンシンセターゼ遺伝子の発現を不活性化する方法及び組成物 |
| EP2674495A1 (en) * | 2012-06-14 | 2013-12-18 | Sanofi | CHO expression system |
| AU2015334872B2 (en) * | 2014-10-23 | 2021-12-16 | Sanofi | Novel selection marker for cell transfection and protein production |
| US12497624B2 (en) * | 2018-07-13 | 2025-12-16 | Lonza Ltd | Methods for improving production of biological products by reducing the level of endogenous protein |
| WO2020012446A1 (en) * | 2018-07-13 | 2020-01-16 | Enzene Biosciences Limited | Double knock-out cho cell line method of its generation and producing therapeutic proteins therefrom |
| US12529071B2 (en) * | 2019-03-19 | 2026-01-20 | Sanofi | Selection marker-comprising cell line and uses thereof for protein production |
| WO2020243642A2 (en) * | 2019-05-31 | 2020-12-03 | The Trustees Of Columbia University In The City Of New York | Multiply auxotrophic cell line for the production of recombinant proteins and methods thereof |
| CN110343668B (zh) * | 2019-07-23 | 2021-11-19 | 珠海联邦制药股份有限公司 | 一种敲除gs基因的cho细胞株及其制备方法与应用 |
-
2021
- 2021-09-17 TW TW110134938A patent/TWI912369B/zh active
- 2021-09-21 PL PL21777795.2T patent/PL4214308T3/pl unknown
- 2021-09-21 ES ES21777795T patent/ES3010648T3/es active Active
- 2021-09-21 AU AU2021342718A patent/AU2021342718A1/en active Pending
- 2021-09-21 WO PCT/EP2021/075864 patent/WO2022058603A1/en not_active Ceased
- 2021-09-21 CA CA3195524A patent/CA3195524A1/en active Pending
- 2021-09-21 EP EP21777795.2A patent/EP4214308B1/en active Active
- 2021-09-21 MX MX2023003278A patent/MX2023003278A/es unknown
- 2021-09-21 IL IL301442A patent/IL301442A/en unknown
- 2021-09-21 BR BR112023005002A patent/BR112023005002A2/pt unknown
- 2021-09-21 US US18/245,872 patent/US20240043878A1/en active Pending
- 2021-09-21 HU HUE21777795A patent/HUE070250T2/hu unknown
- 2021-09-21 KR KR1020237009323A patent/KR20230068401A/ko active Pending
- 2021-09-21 CN CN202180074963.4A patent/CN116457468A/zh active Pending
- 2021-09-21 EP EP24214023.4A patent/EP4541812A3/en active Pending
- 2021-09-21 JP JP2023518094A patent/JP2023544267A/ja active Pending
-
2023
- 2023-03-15 ZA ZA2023/03597A patent/ZA202303597B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL301442A (en) | 2023-05-01 |
| MX2023003278A (es) | 2023-04-12 |
| EP4214308B1 (en) | 2024-11-20 |
| US20240043878A1 (en) | 2024-02-08 |
| AU2021342718A9 (en) | 2025-03-27 |
| CN116457468A (zh) | 2023-07-18 |
| KR20230068401A (ko) | 2023-05-17 |
| EP4214308A1 (en) | 2023-07-26 |
| HUE070250T2 (hu) | 2025-05-28 |
| PL4214308T3 (pl) | 2025-04-14 |
| TWI912369B (zh) | 2026-01-21 |
| ZA202303597B (en) | 2024-08-28 |
| BR112023005002A2 (pt) | 2023-04-18 |
| ES3010648T3 (en) | 2025-04-04 |
| WO2022058603A1 (en) | 2022-03-24 |
| AU2021342718A1 (en) | 2023-06-08 |
| TW202227622A (zh) | 2022-07-16 |
| JP2023544267A (ja) | 2023-10-23 |
| EP4541812A2 (en) | 2025-04-23 |
| EP4541812A3 (en) | 2025-06-11 |
| EP4214308C0 (en) | 2024-11-20 |
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Legal Events
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