MX2023003278A - Generacion de una linea celular de ovario de hamster chino recombinante de alta produccion para la produccion de proteinas terapeuticas. - Google Patents
Generacion de una linea celular de ovario de hamster chino recombinante de alta produccion para la produccion de proteinas terapeuticas.Info
- Publication number
- MX2023003278A MX2023003278A MX2023003278A MX2023003278A MX2023003278A MX 2023003278 A MX2023003278 A MX 2023003278A MX 2023003278 A MX2023003278 A MX 2023003278A MX 2023003278 A MX2023003278 A MX 2023003278A MX 2023003278 A MX2023003278 A MX 2023003278A
- Authority
- MX
- Mexico
- Prior art keywords
- cell line
- generation
- producing recombinant
- chinese hamster
- hamster ovary
- Prior art date
Links
- 210000004978 chinese hamster ovary cell Anatomy 0.000 title 1
- 230000001225 therapeutic effect Effects 0.000 title 1
- 230000014616 translation Effects 0.000 title 1
- 108010052167 Dihydroorotate Dehydrogenase Proteins 0.000 abstract 2
- 102100032823 Dihydroorotate dehydrogenase (quinone), mitochondrial Human genes 0.000 abstract 2
- 102000005396 glutamine synthetase Human genes 0.000 abstract 2
- 108020002326 glutamine synthetase Proteins 0.000 abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
- C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
- C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Reproductive Health (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención se refiere a una línea celular que comprende los genes endógenos de dihidroorotato deshidrogenasa (DHODH) y glutamina sintetasa (GS) que están parcial o totalmente inactivados, y su uso para producir proteínas recombinantes.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP20306068 | 2020-09-21 | ||
PCT/EP2021/075864 WO2022058603A1 (en) | 2020-09-21 | 2021-09-21 | Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2023003278A true MX2023003278A (es) | 2023-04-12 |
Family
ID=72840426
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2023003278A MX2023003278A (es) | 2020-09-21 | 2021-09-21 | Generacion de una linea celular de ovario de hamster chino recombinante de alta produccion para la produccion de proteinas terapeuticas. |
Country Status (12)
Country | Link |
---|---|
US (1) | US20240043878A1 (es) |
EP (1) | EP4214308A1 (es) |
JP (1) | JP2023544267A (es) |
KR (1) | KR20230068401A (es) |
CN (1) | CN116457468A (es) |
AU (1) | AU2021342718A1 (es) |
BR (1) | BR112023005002A2 (es) |
CA (1) | CA3195524A1 (es) |
IL (1) | IL301442A (es) |
MX (1) | MX2023003278A (es) |
TW (1) | TW202227622A (es) |
WO (1) | WO2022058603A1 (es) |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
KR100267720B1 (ko) | 1997-12-29 | 2001-03-02 | 박호군 | 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법 |
US20030166201A1 (en) * | 2001-04-30 | 2003-09-04 | Jensen Michael C. | Selection systems for genetically modified cells |
EP2674495A1 (en) | 2012-06-14 | 2013-12-18 | Sanofi | CHO expression system |
SG11201702516UA (en) | 2014-10-23 | 2017-04-27 | Sanofi Sa | Novel selection marker for cell transfection and protein production |
EP3821027A1 (en) * | 2018-07-13 | 2021-05-19 | Enzene Biosciences Ltd. | Double knock-out cho cell line method of its generation and producing therapeutic proteins therefrom |
BR112021018124A2 (pt) * | 2019-03-19 | 2021-11-16 | Sanofi Sa | Linhagem celular compreendendo marcador de seleção e usos do mesmo para produção de proteína |
WO2020243642A2 (en) * | 2019-05-31 | 2020-12-03 | The Trustees Of Columbia University In The City Of New York | Multiply auxotrophic cell line for the production of recombinant proteins and methods thereof |
-
2021
- 2021-09-17 TW TW110134938A patent/TW202227622A/zh unknown
- 2021-09-21 KR KR1020237009323A patent/KR20230068401A/ko unknown
- 2021-09-21 EP EP21777795.2A patent/EP4214308A1/en active Pending
- 2021-09-21 JP JP2023518094A patent/JP2023544267A/ja active Pending
- 2021-09-21 BR BR112023005002A patent/BR112023005002A2/pt unknown
- 2021-09-21 AU AU2021342718A patent/AU2021342718A1/en active Pending
- 2021-09-21 US US18/245,872 patent/US20240043878A1/en active Pending
- 2021-09-21 CN CN202180074963.4A patent/CN116457468A/zh active Pending
- 2021-09-21 MX MX2023003278A patent/MX2023003278A/es unknown
- 2021-09-21 IL IL301442A patent/IL301442A/en unknown
- 2021-09-21 CA CA3195524A patent/CA3195524A1/en active Pending
- 2021-09-21 WO PCT/EP2021/075864 patent/WO2022058603A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
TW202227622A (zh) | 2022-07-16 |
IL301442A (en) | 2023-05-01 |
EP4214308A1 (en) | 2023-07-26 |
KR20230068401A (ko) | 2023-05-17 |
CA3195524A1 (en) | 2022-03-24 |
BR112023005002A2 (pt) | 2023-04-18 |
AU2021342718A1 (en) | 2023-06-08 |
JP2023544267A (ja) | 2023-10-23 |
US20240043878A1 (en) | 2024-02-08 |
WO2022058603A1 (en) | 2022-03-24 |
CN116457468A (zh) | 2023-07-18 |
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