ES299521A1 - Microtome cooling and dehumidifying - Google Patents

Microtome cooling and dehumidifying

Info

Publication number
ES299521A1
ES299521A1 ES0299521A ES299521A ES299521A1 ES 299521 A1 ES299521 A1 ES 299521A1 ES 0299521 A ES0299521 A ES 0299521A ES 299521 A ES299521 A ES 299521A ES 299521 A1 ES299521 A1 ES 299521A1
Authority
ES
Spain
Prior art keywords
liquid
heat sink
tube
immersed
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES0299521A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Whirlpool Corp
Original Assignee
Whirlpool Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Whirlpool Corp filed Critical Whirlpool Corp
Publication of ES299521A1 publication Critical patent/ES299521A1/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D17/00—Arrangements for circulating cooling fluids; Arrangements for circulating gas, e.g. air, within refrigerated spaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T83/00—Cutting
    • Y10T83/283—With means to control or modify temperature of apparatus or work

Abstract

Apparatus for cooling a micistone having a specimen holder 15 and a knife 13 for cutting a specimen 15a comprises an air conduit adapted to receive the holder and knife and means for directing a stream of cooled, dried air through the conduit. The micistone is supported on a table 11 and enclosed in a cover 19 which is hingedly connected to the table. The cooling apparatus is a separate unit comprising a refrigerating system the evaporator 36 of which is immersed in a liquid heat sink 33. The air conduit 41 includes a portion 42 in the hinged cover 19, a portion 43 connecting the portion 42 with the top of the heat sink housing 34 and a cooling chamber 44. The cooled, dried air passes from the heat sink 33 through the portions 43, 42 onto the specimen 15a and subsequently into the chamber 44. Liquid, for example calcium chloride, is pumped from the heat sink 33 through a hollow tube 51 and sprayed into the chamber 44. A blower 58 mounted on the tube 51 draws air through an aperture 47 in a partition 46 of the chamber 44, and returns the air via apertures 50 to the heat sink 33. The sprayed liquid is allanges to flow into a drain-tube 62 having its exit immersed in the liquid in the heat sink. The volume of liquid in the sink 33 is controlled by a float-operated valve 67. When the valve is closed the liquid collected in the tube 62 passes via a tube 70 into a boiler 71. A device for storing specimens in frozen condition preparatory to cutting sections therefrom comprises a reservoir of anti-freeze liquid which is connected in a closed circuit with a coil immersed in the liquid of the heat sink 33, and a magnetically operated pump. The specimens are supported on members having extensions which are immersed in the liquid of the reservoir.
ES0299521A 1963-05-06 1964-05-05 Microtome cooling and dehumidifying Expired ES299521A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US27801463 US3236133A (en) 1963-05-06 1963-05-06 Microtome cooling and dehumidifying

Publications (1)

Publication Number Publication Date
ES299521A1 true ES299521A1 (en) 1964-09-01

Family

ID=23063331

Family Applications (1)

Application Number Title Priority Date Filing Date
ES0299521A Expired ES299521A1 (en) 1963-05-06 1964-05-05 Microtome cooling and dehumidifying

Country Status (5)

Country Link
US (1) US3236133A (en)
CH (1) CH417142A (en)
ES (1) ES299521A1 (en)
GB (1) GB1059772A (en)
NL (1) NL6404958A (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1170796A (en) * 1967-02-17 1969-11-19 Shandon Scient Ind Ltd Improvements in or relating to Microtomes
US3803958A (en) * 1969-06-02 1974-04-16 Moran H Fernandez Ultra thin sectioning with ultra sharp diamond edge at ultra low temperature
US3646841A (en) * 1969-06-02 1972-03-07 Humberto Fernandez Moran Villa Apparatus using ultrasharp diamond edge for ultrathin sectioning
US3894208A (en) * 1971-02-12 1975-07-08 Wilfried Hellmuth Bergmann Method of abrasive machining of material by means of an energy beam
GB1361334A (en) * 1972-04-07 1974-07-24 Imp Metal Ind Kynoch Ltd Method and apparatus for cutting elongate articles
US4091580A (en) * 1977-06-29 1978-05-30 Timex Corporation Process for holding and cutting sheet glass
DE8327637U1 (en) * 1983-09-27 1984-01-05 Parke, Davis & Co., 07950 Morris Plains, N.J. Microtome
DE8812708U1 (en) * 1988-10-10 1989-01-05 Cambridge Instruments Gmbh, 6907 Nussloch, De
US4918941A (en) * 1989-05-16 1990-04-24 Board Of Regents, The University Of Texas System Cryogenic ultramicrotome seal
DE4012600C2 (en) * 1989-05-26 1998-05-14 Leica Ag Microtome
US5533342A (en) * 1995-02-08 1996-07-09 Gordon; Abe Semi-automated cryostat
DE10244055B8 (en) * 2002-09-21 2005-10-20 Microm Int Gmbh Cryostat microtome with a tempering device
JP4840763B2 (en) * 2006-01-18 2011-12-21 セイコーインスツル株式会社 Automatic thin section preparation apparatus and automatic thin section preparation apparatus
GB0707594D0 (en) * 2007-04-19 2007-05-30 Ionoptika Ltd Freeze-fracture sample holder
DE102008047415B4 (en) * 2008-09-16 2010-12-02 Leica Biosystems Nussloch Gmbh Microtome for making cuts of an object
DE102009061068B4 (en) * 2009-04-17 2015-05-07 Leica Biosystems Nussloch Gmbh Method for producing microscopic thin sections of tissue samples
US10222312B2 (en) * 2016-06-28 2019-03-05 Anton Paar Quantatec, Inc. Cryogenic temperature controller for volumetric sorption analyzers

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2292973A (en) * 1941-02-06 1942-08-11 Spencer Lens Co Microtome
US2379932A (en) * 1944-02-18 1945-07-10 Niagara Blower Co Heat exchange device
US2507632A (en) * 1944-11-30 1950-05-16 Eastman Kodak Co Process for dehydrating materials under low-pressure conditions
US2670657A (en) * 1949-06-24 1954-03-02 Rca Corp High speed microtomy
DE1032572B (en) * 1954-06-01 1958-06-19 Hellmuth Sitte Microtome, especially ultramicrotome
US2961908A (en) * 1954-09-04 1960-11-29 Villalobos Hum Fernandez-Moran Microtome
US2859946A (en) * 1955-01-31 1958-11-11 John R Boyle Heat exchange device
US3027734A (en) * 1960-03-18 1962-04-03 Reflectotherm Inc Chilling and freezing systems

Also Published As

Publication number Publication date
CH417142A (en) 1966-07-15
US3236133A (en) 1966-02-22
GB1059772A (en) 1967-02-22
NL6404958A (en) 1964-11-09

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