ES2969671T3 - Obtención de imágenes con multiplexación usando MERFISH, microscopía de expansión y tecnologías relacionadas - Google Patents

Obtención de imágenes con multiplexación usando MERFISH, microscopía de expansión y tecnologías relacionadas Download PDF

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Publication number
ES2969671T3
ES2969671T3 ES17869515T ES17869515T ES2969671T3 ES 2969671 T3 ES2969671 T3 ES 2969671T3 ES 17869515 T ES17869515 T ES 17869515T ES 17869515 T ES17869515 T ES 17869515T ES 2969671 T3 ES2969671 T3 ES 2969671T3
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nucleic acid
probes
expandable material
rna
cells
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English (en)
Spanish (es)
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Xiaowei Zhuang
Jeffrey Moffitt
Guiping Wang
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Harvard College
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Harvard College
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36Embedding or analogous mounting of samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES17869515T 2016-11-08 2017-11-08 Obtención de imágenes con multiplexación usando MERFISH, microscopía de expansión y tecnologías relacionadas Active ES2969671T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662419033P 2016-11-08 2016-11-08
US201762569127P 2017-10-06 2017-10-06
PCT/US2017/060558 WO2018089438A1 (en) 2016-11-08 2017-11-08 Multiplexed imaging using merfish, expansion microscopy, and related technologies

Publications (1)

Publication Number Publication Date
ES2969671T3 true ES2969671T3 (es) 2024-05-21

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ES17869515T Active ES2969671T3 (es) 2016-11-08 2017-11-08 Obtención de imágenes con multiplexación usando MERFISH, microscopía de expansión y tecnologías relacionadas

Country Status (5)

Country Link
US (2) US20190276881A1 (de)
EP (2) EP3538867B1 (de)
CN (2) CN116242687A (de)
ES (1) ES2969671T3 (de)
WO (1) WO2018089438A1 (de)

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Also Published As

Publication number Publication date
US20190276881A1 (en) 2019-09-12
EP3538867A4 (de) 2020-08-19
CN110168346B (zh) 2023-03-03
EP3538867C0 (de) 2024-01-03
CN110168346A (zh) 2019-08-23
EP4339956A3 (de) 2024-06-19
EP3538867B1 (de) 2024-01-03
CN116242687A (zh) 2023-06-09
WO2018089438A8 (en) 2018-07-19
EP3538867A1 (de) 2019-09-18
WO2018089438A1 (en) 2018-05-17
US20240271193A1 (en) 2024-08-15
EP4339956A2 (de) 2024-03-20

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