ES2872048T3 - Métodos celulares para acoplar interacciones de proteínas y selección y diversificación de moléculas de unión - Google Patents
Métodos celulares para acoplar interacciones de proteínas y selección y diversificación de moléculas de unión Download PDFInfo
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
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- G—PHYSICS
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- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
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- G—PHYSICS
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Applications Claiming Priority (1)
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| JP (1) | JP2016511405A (https=) |
| ES (1) | ES2872048T3 (https=) |
| PT (1) | PT3588089T (https=) |
| WO (1) | WO2014133405A2 (https=) |
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| PT3247728T (pt) | 2015-01-20 | 2020-07-16 | Igm Biosciences Inc | Moléculas de ligação do recetor da superfamília do fator de necrose tumoral (fnt) e utilização das mesmas |
| KR102357312B1 (ko) | 2015-03-04 | 2022-02-03 | 아이쥐엠 바이오사이언스 인코포레이티드 | Ｃｄ20 결합 분자 및 그의 용도 |
| EP3824903A1 (en) | 2015-09-30 | 2021-05-26 | IGM Biosciences Inc. | Binding molecules with modified j-chain |
| EP3356401B1 (en) | 2015-09-30 | 2020-06-24 | IGM Biosciences, Inc. | Binding molecules with modified j-chain |
| GB201704115D0 (en) * | 2017-03-15 | 2017-04-26 | Oxford Genetics Ltd | Method of selecting for antibodies |
| CN113388638A (zh) * | 2021-04-13 | 2021-09-14 | 中国人民解放军西部战区总医院 | 一种同时结合TGFβ和VEGF的双靶点融合蛋白质粒的构建方法 |
| CA3248464A1 (en) * | 2022-01-13 | 2023-07-20 | Massachusetts Institute Of Technology | ANALYSIS, SCREENING AND SELECTION OF FUNCTIONS OF SOLUBBLE PROTEINS IN CELLULAR BANK OF SECRETED PROTEINS |
| JP2025516055A (ja) * | 2022-05-03 | 2025-05-26 | エムブレース セラピューティクス，インコーポレーテッド | 組換え抗体の産生方法及び関連する細胞系 |
| WO2023250481A2 (en) * | 2022-06-23 | 2023-12-28 | Fuller Laboratories | Rickettsia igm assay |
| EP4602367A2 (en) * | 2022-10-12 | 2025-08-20 | Massachusetts Institute Of Technology | Library-scale methods for polypeptide functional analysis |
| CN116236584B (zh) * | 2023-02-20 | 2024-03-22 | 四川大学 | 一种用于高效递送siRNA的多糖-多肽偶联物 |
| TW202525834A (zh) * | 2023-12-28 | 2025-07-01 | 大陸商上海藥明生物技術有限公司 | 構建生產去岩藻糖基化蛋白質的細胞的方法及其應用 |
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| US7179462B2 (en) * | 2000-06-02 | 2007-02-20 | University Of Connecticut Health Center | α (2) macroglobulin receptor as a heat shock protein receptor and uses thereof |
| EP1949107A1 (en) * | 2005-11-03 | 2008-07-30 | Fred Hutchinson Cancer Research Center | Negative immunomodulation of immune responses by nkg2d-positive cd4-positive cells |
| WO2010129485A2 (en) * | 2009-05-04 | 2010-11-11 | San Diego State University Foundation | Compositions and methods for identifying enzyme and transport protein inhibitors |
| CA2762837C (en) * | 2009-05-20 | 2021-08-03 | Novimmune S.A. | Synthetic polypeptide libraries and methods for generating naturally diversified polypeptide variants |
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| US20140249044A1 (en) | 2014-09-04 |
| US9487773B2 (en) | 2016-11-08 |
| JP2016511405A (ja) | 2016-04-14 |
| EP2962103A2 (en) | 2016-01-06 |
| EP3588089B1 (en) | 2021-03-10 |
| WO2014133405A2 (en) | 2014-09-04 |
| PT3588089T (pt) | 2021-06-02 |
| EP3588089A1 (en) | 2020-01-01 |
| WO2014133405A3 (en) | 2015-02-26 |
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