PT3588089T - Métodos baseados em células para acoplamento de interações de proteínas e seleção e diversificação de moléculas de ligação - Google Patents
Métodos baseados em células para acoplamento de interações de proteínas e seleção e diversificação de moléculas de ligaçãoInfo
- Publication number
- PT3588089T PT3588089T PT190200493T PT19020049T PT3588089T PT 3588089 T PT3588089 T PT 3588089T PT 190200493 T PT190200493 T PT 190200493T PT 19020049 T PT19020049 T PT 19020049T PT 3588089 T PT3588089 T PT 3588089T
- Authority
- PT
- Portugal
- Prior art keywords
- diversification
- cell
- binding molecule
- based methods
- protein interactions
- Prior art date
Links
- 230000008878 coupling Effects 0.000 title 1
- 238000010168 coupling process Methods 0.000 title 1
- 238000005859 coupling reaction Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 230000006916 protein interaction Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361771562P | 2013-03-01 | 2013-03-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT3588089T true PT3588089T (pt) | 2021-06-02 |
Family
ID=50680097
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT190200493T PT3588089T (pt) | 2013-03-01 | 2014-03-03 | Métodos baseados em células para acoplamento de interações de proteínas e seleção e diversificação de moléculas de ligação |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9487773B2 (https=) |
| EP (2) | EP2962103A2 (https=) |
| JP (1) | JP2016511405A (https=) |
| ES (1) | ES2872048T3 (https=) |
| PT (1) | PT3588089T (https=) |
| WO (1) | WO2014133405A2 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL292311A (en) | 2014-04-03 | 2022-06-01 | Igm Biosciences Inc | J-chain qualified |
| PT3247728T (pt) | 2015-01-20 | 2020-07-16 | Igm Biosciences Inc | Moléculas de ligação do recetor da superfamília do fator de necrose tumoral (fnt) e utilização das mesmas |
| KR102357312B1 (ko) | 2015-03-04 | 2022-02-03 | 아이쥐엠 바이오사이언스 인코포레이티드 | Ｃｄ20 결합 분자 및 그의 용도 |
| EP3824903A1 (en) | 2015-09-30 | 2021-05-26 | IGM Biosciences Inc. | Binding molecules with modified j-chain |
| EP3356401B1 (en) | 2015-09-30 | 2020-06-24 | IGM Biosciences, Inc. | Binding molecules with modified j-chain |
| GB201704115D0 (en) * | 2017-03-15 | 2017-04-26 | Oxford Genetics Ltd | Method of selecting for antibodies |
| CN113388638A (zh) * | 2021-04-13 | 2021-09-14 | 中国人民解放军西部战区总医院 | 一种同时结合TGFβ和VEGF的双靶点融合蛋白质粒的构建方法 |
| CA3248464A1 (en) * | 2022-01-13 | 2023-07-20 | Massachusetts Institute Of Technology | ANALYSIS, SCREENING AND SELECTION OF FUNCTIONS OF SOLUBBLE PROTEINS IN CELLULAR BANK OF SECRETED PROTEINS |
| JP2025516055A (ja) * | 2022-05-03 | 2025-05-26 | エムブレース セラピューティクス，インコーポレーテッド | 組換え抗体の産生方法及び関連する細胞系 |
| WO2023250481A2 (en) * | 2022-06-23 | 2023-12-28 | Fuller Laboratories | Rickettsia igm assay |
| EP4602367A2 (en) * | 2022-10-12 | 2025-08-20 | Massachusetts Institute Of Technology | Library-scale methods for polypeptide functional analysis |
| CN116236584B (zh) * | 2023-02-20 | 2024-03-22 | 四川大学 | 一种用于高效递送siRNA的多糖-多肽偶联物 |
| TW202525834A (zh) * | 2023-12-28 | 2025-07-01 | 大陸商上海藥明生物技術有限公司 | 構建生產去岩藻糖基化蛋白質的細胞的方法及其應用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3832847B2 (ja) * | 1992-07-17 | 2006-10-11 | ダナ−ファーバー キャンサー インスティテュート | 標的分子の細胞内結合方法 |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US7179462B2 (en) * | 2000-06-02 | 2007-02-20 | University Of Connecticut Health Center | α (2) macroglobulin receptor as a heat shock protein receptor and uses thereof |
| EP1949107A1 (en) * | 2005-11-03 | 2008-07-30 | Fred Hutchinson Cancer Research Center | Negative immunomodulation of immune responses by nkg2d-positive cd4-positive cells |
| WO2010129485A2 (en) * | 2009-05-04 | 2010-11-11 | San Diego State University Foundation | Compositions and methods for identifying enzyme and transport protein inhibitors |
| CA2762837C (en) * | 2009-05-20 | 2021-08-03 | Novimmune S.A. | Synthetic polypeptide libraries and methods for generating naturally diversified polypeptide variants |
-
2014
- 2014-02-28 US US14/193,679 patent/US9487773B2/en not_active Expired - Fee Related
- 2014-03-03 JP JP2015560136A patent/JP2016511405A/ja active Pending
- 2014-03-03 PT PT190200493T patent/PT3588089T/pt unknown
- 2014-03-03 EP EP14722391.1A patent/EP2962103A2/en not_active Withdrawn
- 2014-03-03 EP EP19020049.3A patent/EP3588089B1/en not_active Not-in-force
- 2014-03-03 WO PCT/PT2014/000016 patent/WO2014133405A2/en not_active Ceased
- 2014-03-03 ES ES19020049T patent/ES2872048T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20140249044A1 (en) | 2014-09-04 |
| US9487773B2 (en) | 2016-11-08 |
| JP2016511405A (ja) | 2016-04-14 |
| EP2962103A2 (en) | 2016-01-06 |
| EP3588089B1 (en) | 2021-03-10 |
| WO2014133405A2 (en) | 2014-09-04 |
| ES2872048T3 (es) | 2021-11-02 |
| EP3588089A1 (en) | 2020-01-01 |
| WO2014133405A3 (en) | 2015-02-26 |
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