ES2789000T3 - Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente - Google Patents
Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente Download PDFInfo
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- ES2789000T3 ES2789000T3 ES15849196T ES15849196T ES2789000T3 ES 2789000 T3 ES2789000 T3 ES 2789000T3 ES 15849196 T ES15849196 T ES 15849196T ES 15849196 T ES15849196 T ES 15849196T ES 2789000 T3 ES2789000 T3 ES 2789000T3
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
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- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
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| US201462062256P | 2014-10-10 | 2014-10-10 | |
| PCT/US2015/054756 WO2016057829A1 (en) | 2014-10-10 | 2015-10-08 | Nanopore-based polymer analysis with mutually-quenching fluorescent labels |
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| WO2017123647A1 (en) | 2016-01-15 | 2017-07-20 | Quantapore, Inc. | Optically-based nanopore analysis with reduced background |
| WO2017180319A1 (en) * | 2016-04-14 | 2017-10-19 | Quantapore, Inc. | Mixed optical signals in polymer analysis with nanopores |
| JP2019522983A (ja) * | 2016-07-05 | 2019-08-22 | クアンタポール， インコーポレイテッド | 光学ベースのナノポア配列決定 |
| CA3031812A1 (en) * | 2016-08-19 | 2018-02-22 | Quantapore, Inc. | Optically-based nanopore sequencing using quenching agents |
| JP2019528776A (ja) * | 2016-10-10 | 2019-10-17 | クアンタポール， インコーポレイテッド | 光学に基づくナノポア配列決定における蛍光標識組成物の制御 |
| LU100392B1 (en) * | 2017-07-27 | 2019-02-12 | Wisconsin Alumni Res Foundation Warf | A method to determine the throughput speed of a pore |
| CN110243728B (zh) * | 2018-03-07 | 2023-02-10 | 中国石油天然气股份有限公司 | 一种乳液液滴粒径分布的确定方法及用于标定乳液的化合物 |
| DE102019200929A1 (de) * | 2019-01-25 | 2020-07-30 | Robert Bosch Gmbh | Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls |
| WO2022005957A1 (en) * | 2020-06-30 | 2022-01-06 | Dimensiongen | Devices and methods for genomic structural analysis |
| CN113533275B (zh) * | 2021-06-30 | 2023-11-28 | 东南大学 | 固态纳米孔-荧光共振能量转移复合检测方法及系统 |
Family Cites Families (220)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH614781A5 (https=) | 1977-06-27 | 1979-12-14 | Contraves Ag | |
| US5821058A (en) | 1984-01-16 | 1998-10-13 | California Institute Of Technology | Automated DNA sequencing technique |
| US4962037A (en) | 1987-10-07 | 1990-10-09 | United States Of America | Method for rapid base sequencing in DNA and RNA |
| US5131755A (en) | 1988-02-19 | 1992-07-21 | Chadwick Curt H | Automatic high speed optical inspection system |
| US5126022A (en) | 1990-02-28 | 1992-06-30 | Soane Tecnologies, Inc. | Method and device for moving molecules by the application of a plurality of electrical fields |
| US6054034A (en) | 1990-02-28 | 2000-04-25 | Aclara Biosciences, Inc. | Acrylic microchannels and their use in electrophoretic applications |
| JP3123249B2 (ja) | 1991-09-10 | 2001-01-09 | 株式会社日立製作所 | Ｄｎａ分子の長さ計測方法および計測装置 |
| US5405747A (en) * | 1991-09-25 | 1995-04-11 | The Regents Of The University Of California Office Of Technology Transfer | Method for rapid base sequencing in DNA and RNA with two base labeling |
| EP0636186B1 (en) | 1992-04-03 | 1998-11-25 | The Perkin-Elmer Corporation | Probe composition and method |
| US5470705A (en) | 1992-04-03 | 1995-11-28 | Applied Biosystems, Inc. | Probe composition containing a binding domain and polymer chain and methods of use |
| US5498392A (en) | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5387926A (en) | 1992-06-30 | 1995-02-07 | California Institute Of Technology | High speed digital framing camera |
| US5798042A (en) | 1994-03-07 | 1998-08-25 | Regents Of The University Of California | Microfabricated filter with specially constructed channel walls, and containment well and capsule constructed with such filters |
| US6001229A (en) | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6794127B1 (en) | 1997-06-16 | 2004-09-21 | Diversa Corporation | Capillary array-based sample screening |
| WO1997039150A1 (en) | 1996-04-15 | 1997-10-23 | University Of Southern California | Synthesis of fluorophore-labeled dna |
| US6399023B1 (en) | 1996-04-16 | 2002-06-04 | Caliper Technologies Corp. | Analytical system and method |
| US5945526A (en) | 1996-05-03 | 1999-08-31 | Perkin-Elmer Corporation | Energy transfer dyes with enhanced fluorescence |
| US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| US6136543A (en) | 1997-01-31 | 2000-10-24 | Hitachi, Ltd. | Method for determining nucleic acids base sequence and apparatus therefor |
| WO1998035012A2 (en) | 1997-02-12 | 1998-08-13 | Chan Eugene Y | Methods and products for analyzing polymers |
| DE19714221A1 (de) | 1997-04-07 | 1998-10-08 | Zeiss Carl Fa | Konfokales Mikroskop mit einem motorischen Scanningtisch |
| CZ297165B6 (cs) | 1997-04-21 | 2006-09-13 | Randox Laboratories Ltd. A British Company Of Ardmore | Zpusob výroby zarízení s pevnou fází k provádení multianalytních rozboru |
| AU8586298A (en) | 1997-07-25 | 1999-02-16 | University Of Massachusetts | Designed protein pores as components for biosensors |
| AU1517999A (en) | 1997-10-15 | 1999-05-03 | Aclara Biosciences, Inc. | Laminate microstructure device and method for making same |
| AU1603199A (en) | 1997-12-03 | 1999-06-16 | Curagen Corporation | Methods and devices for measuring differential gene expression |
| US20020144905A1 (en) | 1997-12-17 | 2002-10-10 | Christian Schmidt | Sample positioning and analysis system |
| US7244349B2 (en) | 1997-12-17 | 2007-07-17 | Molecular Devices Corporation | Multiaperture sample positioning and analysis system |
| EP1040349B2 (de) | 1997-12-17 | 2012-12-19 | Ecole Polytechnique Federale De Lausanne (Epfl) | Positionierung und elektrophysiologische charakterisierung einzelner zellen und rekonstituierter membransysteme auf mikrostrukturierten trägern |
| CN1248741C (zh) | 1997-12-23 | 2006-04-05 | 斯特里斯公司 | 具有集成过滤器的抗菌混合剂输送系统 |
| US6265163B1 (en) | 1998-01-09 | 2001-07-24 | Lynx Therapeutics, Inc. | Solid phase selection of differentially expressed genes |
| DE19804372A1 (de) | 1998-02-04 | 1999-08-05 | Michael W Dr Dr Dahm | Verfahren zur quantitativen Bestimmung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits |
| GB9805301D0 (en) | 1998-03-12 | 1998-05-06 | Imperial College | Detector |
| US6306589B1 (en) | 1998-05-27 | 2001-10-23 | Vysis, Inc. | Biological assays for analyte detection |
| US6504943B1 (en) | 1998-07-20 | 2003-01-07 | Sandia Corporation | Information-efficient spectral imaging sensor |
| US6447724B1 (en) | 1998-08-11 | 2002-09-10 | Caliper Technologies Corp. | DNA sequencing using multiple fluorescent labels being distinguishable by their decay times |
| US6210896B1 (en) | 1998-08-13 | 2001-04-03 | Us Genomics | Molecular motors |
| US6790671B1 (en) | 1998-08-13 | 2004-09-14 | Princeton University | Optically characterizing polymers |
| US6263286B1 (en) | 1998-08-13 | 2001-07-17 | U.S. Genomics, Inc. | Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer |
| US6906749B1 (en) | 1998-09-16 | 2005-06-14 | Dalsa, Inc. | CMOS TDI image sensor |
| EP1271154A3 (en) | 1998-09-18 | 2005-08-17 | Massachusetts Institute Of Technology | Biological applications of semiconductor nanocrystals |
| US6251303B1 (en) | 1998-09-18 | 2001-06-26 | Massachusetts Institute Of Technology | Water-soluble fluorescent nanocrystals |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US6426231B1 (en) | 1998-11-18 | 2002-07-30 | The Texas A&M University System | Analyte sensing mediated by adapter/carrier molecules |
| US6252303B1 (en) | 1998-12-02 | 2001-06-26 | Advanced Micro Devices, Inc. | Intergration of low-K SiOF as inter-layer dielectric |
| WO2000034527A2 (en) | 1998-12-11 | 2000-06-15 | The Regents Of The University Of California | Targeted molecular bar codes |
| FR2788008B1 (fr) | 1998-12-30 | 2001-03-23 | Inst Curie | Milieu thermosensible pour la separation electrocinetique d'especes au sein d'un canal de separation |
| WO2000043547A1 (en) | 1999-01-20 | 2000-07-27 | Northwestern University | Dna mobility modifier |
| US6671044B2 (en) | 1999-01-25 | 2003-12-30 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells in broad flat flow |
| US6249341B1 (en) | 1999-01-25 | 2001-06-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6473176B2 (en) | 1999-01-25 | 2002-10-29 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6608682B2 (en) | 1999-01-25 | 2003-08-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6975400B2 (en) | 1999-01-25 | 2005-12-13 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| WO2000056937A2 (en) | 1999-03-25 | 2000-09-28 | Hyseq, Inc. | Solution-based methods and materials for sequence analysis by hybridization |
| US20040214221A1 (en) | 1999-05-07 | 2004-10-28 | Klaus Muehlegger | High density labeling of DNA with modified or "chromophore" carrying nucleotides and DNA polymerases used |
| US7122152B2 (en) | 1999-05-10 | 2006-10-17 | University Of Florida | Spatiotemporal and geometric optimization of sensor arrays for detecting analytes fluids |
| JP3815969B2 (ja) | 1999-05-12 | 2006-08-30 | アクララ バイオサイエンシーズ， インコーポレイテッド | 微量流体デバイスにおける多重方式蛍光検出 |
| US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| EP2383776B1 (en) | 1999-06-22 | 2015-02-25 | President and Fellows of Harvard College | Solid state nanopore device for evaluating biopolymers |
| US8206568B2 (en) | 1999-06-22 | 2012-06-26 | President And Fellows Of Harvard College | Material deposition techniques for control of solid state aperture surface properties |
| US6464842B1 (en) | 1999-06-22 | 2002-10-15 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US6982146B1 (en) | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
| AU7105300A (en) | 1999-09-03 | 2001-04-10 | Lifebeam Technologies, Inc. | Optical system for rapid polymer analysis |
| EP1212462B1 (en) | 1999-09-07 | 2006-01-25 | The Regents of the University of California | Methods of determining the presence of double stranded nucleic acids in a sample |
| US6335420B1 (en) | 1999-11-22 | 2002-01-01 | Ibc Advanced Technologies, Inc. | Polyamide ligand-containing polymeric resins and methods of using the same for removing, separating and/or concentrating desired metal ions from solutions |
| CA2398107C (en) | 2000-01-28 | 2013-11-19 | Althea Technologies, Inc. | Methods for analysis of gene expression |
| WO2001059453A2 (en) | 2000-02-11 | 2001-08-16 | The Texas A & M University System | Biosensor compositions and methods of use |
| US6616895B2 (en) | 2000-03-23 | 2003-09-09 | Advanced Research Corporation | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| US20060292041A1 (en) | 2000-03-23 | 2006-12-28 | Dugas Matthew P | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US6917726B2 (en) | 2001-09-27 | 2005-07-12 | Cornell Research Foundation, Inc. | Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes |
| US20050153284A1 (en) | 2000-06-30 | 2005-07-14 | Zeno Foldes-Papp | Single molecule sequencing method |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| US6934408B2 (en) | 2000-08-25 | 2005-08-23 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
| US6583865B2 (en) | 2000-08-25 | 2003-06-24 | Amnis Corporation | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
| US6608680B2 (en) | 2000-08-25 | 2003-08-19 | Amnis Corporation | TDI imaging system for kinetic studies |
| AU2001293232A1 (en) | 2000-08-29 | 2002-03-13 | The Rockefeller University | Methods employing fluorescence quenching by metal surfaces |
| US6939451B2 (en) | 2000-09-19 | 2005-09-06 | Aclara Biosciences, Inc. | Microfluidic chip having integrated electrodes |
| WO2002042496A2 (en) | 2000-11-27 | 2002-05-30 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US6998251B2 (en) | 2001-01-12 | 2006-02-14 | Syngenta Participations Ag | Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics |
| EP1384022A4 (en) | 2001-04-06 | 2004-08-04 | California Inst Of Techn | NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES |
| US6743905B2 (en) | 2001-04-16 | 2004-06-01 | Applera Corporation | Mobility-modified nucleobase polymers and methods of using same |
| KR100889351B1 (ko) | 2001-05-15 | 2009-03-18 | 가부시키가이샤 에바라 세이사꾸쇼 | Ｔｄｉ 검출 디바이스, 피드-스루 장비 및 이들 디바이스를 이용하는 전자빔 장치 |
| US20040175710A1 (en) | 2001-05-22 | 2004-09-09 | Haushalter Robert C. | Method for in situ, on-chip chemical synthesis |
| US6618140B2 (en) | 2001-06-18 | 2003-09-09 | Amnis Corporation | Spectral deconvolution of fluorescent markers |
| US7009163B2 (en) | 2001-06-22 | 2006-03-07 | Orbotech Ltd. | High-sensitivity optical scanning using memory integration |
| WO2003003015A2 (en) | 2001-06-28 | 2003-01-09 | Advanced Research And Technology Institute, Inc. | Methods of preparing multicolor quantum dot tagged beads and conjugates thereof |
| JP2004537053A (ja) | 2001-07-25 | 2004-12-09 | アプレラ コーポレイション | 電気泳動検出システムにおける時間遅延積分 |
| US7280207B2 (en) | 2001-07-25 | 2007-10-09 | Applera Corporation | Time-delay integration in a flow cytometry system |
| WO2003010289A2 (en) | 2001-07-25 | 2003-02-06 | The Trustees Of Princeton University | Nanochannel arrays and their preparation and use for high throughput macromolecular analysis |
| US20080288178A1 (en) | 2001-08-24 | 2008-11-20 | Applera Corporation | Sequencing system with memory |
| US6627433B2 (en) | 2001-08-24 | 2003-09-30 | Applera Corporation | Multi-channel analyte-separation device employing side-entry excitation |
| US6852492B2 (en) | 2001-09-24 | 2005-02-08 | Intel Corporation | Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication |
| US6706203B2 (en) | 2001-10-30 | 2004-03-16 | Agilent Technologies, Inc. | Adjustable nanopore, nanotome, and nanotweezer |
| DE10154291B4 (de) | 2001-11-05 | 2005-05-19 | Hain Lifescience Gmbh | Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes |
| DE10162535A1 (de) | 2001-12-19 | 2003-07-03 | Gnothis Holding Sa Ecublens | Sequenzierung über Lochmembranen |
| US7008547B2 (en) | 2002-03-14 | 2006-03-07 | Sarnoff Corporation | Solid phase sensors |
| JP3752466B2 (ja) | 2002-04-24 | 2006-03-08 | 株式会社日立製作所 | 遺伝子検査方法 |
| US8278055B2 (en) | 2002-05-01 | 2012-10-02 | Intel Corporation | Methods and device for analyte characterization |
| US7744816B2 (en) | 2002-05-01 | 2010-06-29 | Intel Corporation | Methods and device for biomolecule characterization |
| EP1504114B1 (en) | 2002-05-10 | 2017-07-12 | The Texas A & M University System | Stochastic sensing through covalent interactions |
| US7005264B2 (en) | 2002-05-20 | 2006-02-28 | Intel Corporation | Method and apparatus for nucleic acid sequencing and identification |
| US20050019784A1 (en) | 2002-05-20 | 2005-01-27 | Xing Su | Method and apparatus for nucleic acid sequencing and identification |
| US6952651B2 (en) | 2002-06-17 | 2005-10-04 | Intel Corporation | Methods and apparatus for nucleic acid sequencing by signal stretching and data integration |
| TWI234584B (en) | 2002-08-16 | 2005-06-21 | Univ Nat Taiwan Normal | Nucleic acid sequencing method |
| WO2004029580A2 (en) | 2002-09-25 | 2004-04-08 | Amersham Biosciences (Sv) Corp | High density fluidic chip design and method of sample injection |
| AU2003301244A1 (en) | 2002-10-15 | 2004-05-04 | Advanced Research Corporation | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| CN1161613C (zh) | 2002-10-22 | 2004-08-11 | 中国人民解放军南京军区联勤部军事医学研究所 | 免疫细胞微流体阵列 |
| US7390457B2 (en) | 2002-10-31 | 2008-06-24 | Agilent Technologies, Inc. | Integrated microfluidic array device |
| US20040137158A1 (en) | 2003-01-15 | 2004-07-15 | Kools Jacques Constant Stefan | Method for preparing a noble metal surface |
| US7410564B2 (en) | 2003-01-27 | 2008-08-12 | Agilent Technologies, Inc. | Apparatus and method for biopolymer identification during translocation through a nanopore |
| CA2513899C (en) | 2003-01-29 | 2013-03-26 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| WO2004085609A2 (en) | 2003-02-28 | 2004-10-07 | Brown University | Nanopores, methods for using same, methods for making same and methods for characterizing biomolecules using same |
| US7250115B2 (en) | 2003-06-12 | 2007-07-31 | Agilent Technologies, Inc | Nanopore with resonant tunneling electrodes |
| US7444053B2 (en) | 2003-06-16 | 2008-10-28 | The Regents Of The University Of California | Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity |
| US7248771B2 (en) | 2003-06-16 | 2007-07-24 | Brigham Young University | Integrated sensor with electrical and optical single molecule sensitivity |
| WO2005017025A2 (en) | 2003-08-15 | 2005-02-24 | The President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US7075161B2 (en) | 2003-10-23 | 2006-07-11 | Agilent Technologies, Inc. | Apparatus and method for making a low capacitance artificial nanopore |
| US20050095599A1 (en) | 2003-10-30 | 2005-05-05 | Pittaro Richard J. | Detection and identification of biopolymers using fluorescence quenching |
| US20050136408A1 (en) | 2003-12-19 | 2005-06-23 | May Tom-Moy | Methods and systems for characterizing a polymer |
| US7271896B2 (en) | 2003-12-29 | 2007-09-18 | Intel Corporation | Detection of biomolecules using porous biosensors and raman spectroscopy |
| US20050164211A1 (en) | 2004-01-22 | 2005-07-28 | Hannah Eric C. | Carbon nanotube molecular labels |
| WO2005071110A2 (en) | 2004-01-23 | 2005-08-04 | Lingvitae As | Improving polynucleotide ligation reactions |
| US20050186576A1 (en) | 2004-02-19 | 2005-08-25 | Intel Corporation | Polymer sequencing using selectively labeled monomers and data integration |
| US7279337B2 (en) | 2004-03-10 | 2007-10-09 | Agilent Technologies, Inc. | Method and apparatus for sequencing polymers through tunneling conductance variation detection |
| US7238485B2 (en) | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US20050227239A1 (en) | 2004-04-08 | 2005-10-13 | Joyce Timothy H | Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes |
| US20070264623A1 (en) | 2004-06-15 | 2007-11-15 | President And Fellows Of Harvard College | Nanosensors |
| US20060019259A1 (en) | 2004-07-22 | 2006-01-26 | Joyce Timothy H | Characterization of biopolymers by resonance tunneling and fluorescence quenching |
| JP4897681B2 (ja) | 2004-07-23 | 2012-03-14 | エレクトロニック・バイオサイエンシーズ・エルエルシー | イオン・チャネルを通過する時間的に変化する電流を検出するための方法及び装置 |
| US20070054276A1 (en) | 2004-08-12 | 2007-03-08 | Sampson Jeffrey R | Polynucleotide analysis and methods of using nanopores |
| US7972858B2 (en) | 2004-08-13 | 2011-07-05 | President And Fellows Of Harvard College | Ultra high-throughput opti-nanopore DNA readout platform |
| CA2579150C (en) | 2004-09-17 | 2014-11-25 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
| WO2006137891A2 (en) | 2004-09-29 | 2006-12-28 | University Of Florida Research Foundation, Inc. | Membrane with nanochannels for detection of molecules |
| US7371533B2 (en) | 2004-10-05 | 2008-05-13 | University Of Ottawa | Methods for separation of polymeric compounds |
| WO2006052882A1 (en) | 2004-11-09 | 2006-05-18 | President And Fellows Of Harvard College | Formation of eddies in constrained fluidic channels and uses thereof |
| US7609309B2 (en) | 2004-11-18 | 2009-10-27 | Kla-Tencor Technologies Corporation | Continuous clocking of TDI sensors |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US20060210995A1 (en) | 2005-03-15 | 2006-09-21 | Joyce Timothy H | Nanopore analysis systems and methods of using nanopore devices |
| ATE529734T1 (de) | 2005-04-06 | 2011-11-15 | Harvard College | Molekulare charakterisierung mit kohlenstoff- nanoröhrchen-steuerung |
| US20060231419A1 (en) | 2005-04-15 | 2006-10-19 | Barth Philip W | Molecular resonant tunneling sensor and methods of fabricating and using the same |
| US7879575B2 (en) | 2005-04-27 | 2011-02-01 | The Trustees Of The University Of Pennsylvania | Nanostructures that provide a modified nanoenvironment for the enhancement of luminescence |
| EP1721657A1 (en) | 2005-05-13 | 2006-11-15 | SONY DEUTSCHLAND GmbH | A method of fabricating a polymeric membrane having at least one pore |
| JP2007006720A (ja) | 2005-06-28 | 2007-01-18 | Toshiba Corp | 個体識別方法、並びに個体識別検査のためのアレイ、装置及びシステム |
| US20090148348A1 (en) | 2005-08-11 | 2009-06-11 | Eksigent Technologies, Llc | Plastic surfaces and apparatuses for reduced adsorption of solutes and methods of preparing the same |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| WO2007041340A2 (en) | 2005-09-30 | 2007-04-12 | The Trustees Of Columbia University In The City Of New York | Microfluidic cells with parallel arrays of individual dna molecules |
| WO2007041621A2 (en) | 2005-10-03 | 2007-04-12 | Xingsheng Sean Ling | Hybridization assisted nanopore sequencing |
| KR100721430B1 (ko) | 2005-10-12 | 2007-05-23 | 학교법인 포항공과대학교 | 나노다공성 멤브레인 및 이의 제조방법 |
| US7397232B2 (en) | 2005-10-21 | 2008-07-08 | The University Of Akron | Coulter counter having a plurality of channels |
| US7835870B2 (en) | 2005-11-01 | 2010-11-16 | Georgia Institute Of Technology | Methods and systems for evaluating the length of elongated elements |
| WO2007120265A2 (en) | 2005-11-14 | 2007-10-25 | Applera Corporation | Coded molecules for detecting target analytes |
| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| DE602006016984D1 (de) | 2005-12-12 | 2010-10-28 | Us Gov Health & Human Serv | Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren |
| EP1987275A1 (en) | 2006-02-13 | 2008-11-05 | Koninklijke Philips Electronics N.V. | Microfluidic device for molecular diagnostic applications |
| US20070224613A1 (en) | 2006-02-18 | 2007-09-27 | Strathmann Michael P | Massively Multiplexed Sequencing |
| US20070202008A1 (en) | 2006-02-28 | 2007-08-30 | Schembri Carol T | Systems and methods of lipoprotein size fraction assaying |
| US20070215472A1 (en) | 2006-03-15 | 2007-09-20 | Slater Gary W | Electroosmotic flow for end labelled free solution electrophoresis |
| US20070218494A1 (en) | 2006-03-17 | 2007-09-20 | Gary Slater | Branched polymer lables as drag-tags in free solution electrophoresis |
| US7849581B2 (en) | 2006-05-05 | 2010-12-14 | University Of Utah Research Foundation | Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof |
| US7777505B2 (en) | 2006-05-05 | 2010-08-17 | University Of Utah Research Foundation | Nanopore platforms for ion channel recordings and single molecule detection and analysis |
| JP2009543058A (ja) | 2006-06-30 | 2009-12-03 | アプライド バイオシステムズ， エルエルシー | 結合相互作用を分析する方法 |
| US7553730B2 (en) | 2006-07-14 | 2009-06-30 | Agilent Technologies, Inc. | Methods of fabrication employing nanoscale mandrels |
| US20080032290A1 (en) | 2006-08-03 | 2008-02-07 | Young James E | Nanopore flow cells |
| ES2404665T3 (es) | 2006-09-06 | 2013-05-28 | Medical Research Council | Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante |
| WO2008049795A1 (en) | 2006-10-23 | 2008-05-02 | Flexgen Bv | Method and system for calibrating laser focus and position in micro-arrays |
| GB2457402B (en) | 2006-12-01 | 2011-10-19 | Univ Columbia | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| US7544578B2 (en) | 2007-01-03 | 2009-06-09 | International Business Machines Corporation | Structure and method for stochastic integrated circuit personalization |
| US8003319B2 (en) | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
| US20100035268A1 (en) | 2007-02-21 | 2010-02-11 | Joseph Beechem | Materials and methods for single molecule nucleic acid sequencing |
| US20080254995A1 (en) | 2007-02-27 | 2008-10-16 | Drexel University | Nanopore arrays and sequencing devices and methods thereof |
| EP2156179B1 (en) | 2007-04-04 | 2021-08-18 | The Regents of The University of California | Methods for using a nanopore |
| WO2009020682A2 (en) | 2007-05-08 | 2009-02-12 | The Trustees Of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| CA2689626C (en) | 2007-06-06 | 2016-10-25 | Pacific Biosciences Of California, Inc. | Methods and processes for calling bases in sequence by incorporation methods |
| DK2171088T3 (en) | 2007-06-19 | 2016-01-25 | Stratos Genomics Inc | Nucleic acid sequencing in a high yield by expansion |
| GB0713143D0 (en) | 2007-07-06 | 2007-08-15 | Ucl Business Plc | Nucleic acid detection method |
| GB0717150D0 (en) | 2007-09-04 | 2007-10-17 | Univ Warwick | Apparatus and method |
| US8273532B2 (en) | 2007-10-02 | 2012-09-25 | President And Fellows Of Harvard College | Capture, recapture, and trapping of molecules with a nanopore |
| GB0721340D0 (en) | 2007-10-30 | 2007-12-12 | Isis Innovation | Polymerase-based single-molecule DNA sequencing |
| US8852864B2 (en) | 2008-01-17 | 2014-10-07 | Sequenom Inc. | Methods and compositions for the analysis of nucleic acids |
| WO2009108914A1 (en) | 2008-02-28 | 2009-09-03 | Electronic Bio Sciences, Llc | System and method to improve sequencing accuracy of a polymer |
| EP4230747A3 (en) | 2008-03-28 | 2023-11-15 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
| CN102084003A (zh) | 2008-04-04 | 2011-06-01 | 生命科技公司 | 用于成像和测序的扫描系统和方法 |
| US20110172404A1 (en) | 2008-05-19 | 2011-07-14 | Cornell University | Self-Assembly of Nanoparticles Through Nuclei Acid Engineering |
| US8013300B2 (en) | 2008-06-20 | 2011-09-06 | Carl Zeiss Nts, Llc | Sample decontamination |
| US8628919B2 (en) | 2008-06-30 | 2014-01-14 | Bionano Genomics, Inc. | Methods and devices for single-molecule whole genome analysis |
| EP2310534B1 (en) | 2008-07-07 | 2018-09-05 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| ATE544063T1 (de) | 2008-07-17 | 2012-02-15 | Koninkl Philips Electronics Nv | Nanoporenvorrichtung und verfahren zur nukleinsäureanalyse |
| CA2774710C (en) | 2008-09-22 | 2016-08-02 | University Of Washington | Msp nanopores and related methods |
| WO2010036287A1 (en) | 2008-09-24 | 2010-04-01 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| CN201302544Y (zh) | 2008-12-04 | 2009-09-02 | 浙江大学 | 高俘获率高灵敏度的微流控spr生物传感装置 |
| WO2010080617A2 (en) | 2008-12-19 | 2010-07-15 | The Board Of Trustees Of The University Of Illinois | Detecting and sorting methylated dna using a synthetic nanopore |
| CN101519695B (zh) * | 2009-02-19 | 2012-04-18 | 中国人民解放军第三军医大学第一附属医院 | 多靶标量子点标记核酸芯片及其制备方法和检测方法 |
| CA2756233A1 (en) | 2009-03-26 | 2010-09-30 | Trustees Of Boston University | Method for imaging on thin solid-state interface between two fluids |
| JP5372570B2 (ja) | 2009-03-30 | 2013-12-18 | 株式会社日立ハイテクノロジーズ | ナノポアを用いたバイオポリマー決定方法、システム、及びキット |
| US8986928B2 (en) | 2009-04-10 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| WO2010129869A1 (en) | 2009-05-07 | 2010-11-11 | The Trustees Of Boston University | Manufacture of nanoparticles using nanopores and voltage-driven electrolyte flow |
| US8926904B2 (en) | 2009-05-12 | 2015-01-06 | Daniel Wai-Cheong So | Method and apparatus for the analysis and identification of molecules |
| US9255002B2 (en) | 2009-09-22 | 2016-02-09 | Cornell University | Apparatus and method for forming self-assembly arrays |
| EP2483680A4 (en) * | 2009-09-30 | 2014-01-01 | Quantapore Inc | ULTRASOUND SEQUENCING OF BIOLOGICAL POLYMERS WITH THE HELP OF A MARKED NANOPORE |
| US20140335513A9 (en) | 2009-09-30 | 2014-11-13 | Quantapore, Inc. | Hybrid nanopore device with optical detection and methods of using same |
| EP2491138A1 (en) | 2009-10-21 | 2012-08-29 | Bionano Genomics, Inc. | Methods and related devices for single molecule whole genome analysis |
| US9127313B2 (en) | 2009-12-01 | 2015-09-08 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
| CN102918166A (zh) | 2010-03-30 | 2013-02-06 | 波士顿大学董事会 | 用于纳米孔解链依赖性核酸测序的工具和方法 |
| US20130256118A1 (en) | 2010-05-11 | 2013-10-03 | Trustees Of Boston University | Use of Nanopore Arrays For Multiplex Sequencing of Nucleic Acids |
| US8865078B2 (en) | 2010-06-11 | 2014-10-21 | Industrial Technology Research Institute | Apparatus for single-molecule detection |
| CN103069267B (zh) | 2010-09-29 | 2016-05-11 | 株式会社日立高新技术 | 生物聚合物的光学的解析装置以及方法 |
| WO2012121756A1 (en) | 2011-03-04 | 2012-09-13 | Quantapore, Inc. | Apparatus and methods for performing optical nanopore detection or sequencing |
| WO2012170499A2 (en) | 2011-06-06 | 2012-12-13 | The University Of North Carolina At Greensboro | Nanopore fabrication and applications thereof |
| US20130040827A1 (en) | 2011-08-14 | 2013-02-14 | Stephen C. Macevicz | Method and compositions for detecting and sequencing nucleic acids |
| GB201119032D0 (en) | 2011-11-03 | 2011-12-14 | Isis Innovation | Multisomes: encapsulated droplet networks |
| GB2497784A (en) | 2011-12-20 | 2013-06-26 | Base4 Innovation Ltd | Identification of polymers using nanopore devices |
| GB201212135D0 (en) | 2012-07-09 | 2012-08-22 | Base4 Innovation Ltd | Improved sequencing apparatus |
| WO2014066905A2 (en) * | 2012-10-28 | 2014-05-01 | Quantapore, Inc. | Reducing background fluorescence in mems materials by low energy ion beam treatment |
| CA2910019A1 (en) * | 2013-05-24 | 2014-11-27 | Quantapore, Inc. | Nanopore-based nucleic acid analysis with mixed fret detection |
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| EP3204519A1 (en) | 2017-08-16 |
| EP3204519B1 (en) | 2020-04-29 |
| JP6740222B2 (ja) | 2020-08-12 |
| US20200354787A1 (en) | 2020-11-12 |
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