ES2599153T3 - Molécula de ARNi para la timidilato sintasa y uso de la misma - Google Patents

Molécula de ARNi para la timidilato sintasa y uso de la misma Download PDF

Info

Publication number
ES2599153T3
ES2599153T3 ES10758615.8T ES10758615T ES2599153T3 ES 2599153 T3 ES2599153 T3 ES 2599153T3 ES 10758615 T ES10758615 T ES 10758615T ES 2599153 T3 ES2599153 T3 ES 2599153T3
Authority
ES
Spain
Prior art keywords
thymidylate synthase
rnai molecule
seq
nucleotide sequence
rnai
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10758615.8T
Other languages
English (en)
Inventor
Hiromi Wada
Cheng Long HUANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Delta Fly Pharma Inc
Original Assignee
Delta Fly Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Delta Fly Pharma Inc filed Critical Delta Fly Pharma Inc
Application granted granted Critical
Publication of ES2599153T3 publication Critical patent/ES2599153T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088Compounds having three or more nucleosides or nucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4412Non condensed pyridines; Hydrogenated derivatives thereof having oxo groups directly attached to the heterocyclic ring
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/513Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim having oxo groups directly attached to the heterocyclic ring, e.g. cytosine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/53Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with three nitrogens as the only ring hetero atoms, e.g. chlorazanil, melamine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y201/00Transferases transferring one-carbon groups (2.1)
    • C12Y201/01Methyltransferases (2.1.1)
    • C12Y201/01045Thymidylate synthase (2.1.1.45)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering N.A.
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00Applications; Uses
    • C12N2320/30Special therapeutic applications
    • C12N2320/31Combination therapy

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Una molécula de ARNi que es capaz de suprimir la expresión de la timidilato sintasa por la acción de un ARNi que comprende un dominio de ARN de doble cadena compuesto por una cadena con sentido que consiste en la secuencia de nucleótidos representada por SEQ ID NO: 1 hibridada con una cadena antisentido que consiste en la secuencia de nucleótidos representada por SEQ ID NO: 2, en donde la cadena con sentido está unida a la cadena antisentido mediante una región enlazadora.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8

Claims (1)

  1. imagen1
ES10758615.8T 2009-03-31 2010-03-29 Molécula de ARNi para la timidilato sintasa y uso de la misma Active ES2599153T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009086463 2009-03-31
JP2009086463 2009-03-31
PCT/JP2010/055521 WO2010113844A1 (ja) 2009-03-31 2010-03-29 チミジル酸合成酵素に対するRNAi分子およびその用途

Publications (1)

Publication Number Publication Date
ES2599153T3 true ES2599153T3 (es) 2017-01-31

Family

ID=42828134

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10758615.8T Active ES2599153T3 (es) 2009-03-31 2010-03-29 Molécula de ARNi para la timidilato sintasa y uso de la misma

Country Status (14)

Country Link
US (1) US8524876B2 (es)
EP (1) EP2415870B1 (es)
JP (1) JP5687188B2 (es)
KR (1) KR101579638B1 (es)
CN (2) CN107090454A (es)
DK (1) DK2415870T3 (es)
ES (1) ES2599153T3 (es)
HR (1) HRP20161293T1 (es)
HU (1) HUE029171T2 (es)
PL (1) PL2415870T3 (es)
PT (1) PT2415870T (es)
RU (1) RU2535993C2 (es)
TW (1) TWI526211B (es)
WO (1) WO2010113844A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8592572B2 (en) 2011-05-23 2013-11-26 Delta-Fly Pharma, Inc. Liposome containing shRNA molecule targeting a thymidylate synthase and use thereof
US20120301537A1 (en) * 2011-05-23 2012-11-29 Delta-Fly Pharma, Inc. LIPOSOME CONTAINING shRNA MOLECULE TARGETING A THYMIDYLATE SYNTHASE AND USE THEREOF
US20140255471A1 (en) * 2013-03-11 2014-09-11 Wake Forest University Health Sciences Method of treating brain tumors
PL2992874T3 (pl) 2013-04-30 2019-01-31 Delta-Fly Pharma, Inc. Liposomy do podawania miejscowego i ich zastosowanie
JP5934848B1 (ja) 2014-10-30 2016-06-15 Delta−Fly Pharma株式会社 局所投与用リポプレックスの新規製造方法及び該リポプレックスを使用する抗腫瘍剤
JP2023167428A (ja) 2022-05-12 2023-11-24 コニカミノルタ株式会社 光学フィルムの製造方法、光学フィルム、偏光板及び液晶表示装置

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4910510B1 (es) 1968-01-26 1974-03-11
CA2087540C (en) 1991-05-27 1999-01-19 Tetsuhiko Shirasaka Composition, method and kit for potentiating antitumor activity and for curing tumor
RU2279277C2 (ru) * 2000-03-31 2006-07-10 Энджиоджен Фармасьютикалз Лтд. Лечение разделенными дозами агентов с сосудоразрушающей активностью
AU2003236649A1 (en) * 2002-05-15 2003-12-02 Falko Fend Egf receptor antagonists in the treatment of gastric cancer
EP2305812A3 (en) * 2002-11-14 2012-06-06 Dharmacon, Inc. Fuctional and hyperfunctional sirna
JP2007513611A (ja) * 2003-11-26 2007-05-31 ザ クイーンズ ユニヴァーシティ オブ ベルファスト 癌治療
JP4543188B2 (ja) 2004-03-10 2010-09-15 学校法人日本医科大学 ヒトチミジル酸合成酵素に対するRNAiとして作用するRNA配列
KR100844477B1 (ko) * 2004-06-09 2008-07-07 다이호야쿠힌고교 가부시키가이샤 항종양 효과 증강제, 항종양제 및 암 치료 방법
WO2007032428A1 (ja) * 2005-09-14 2007-03-22 Takara Bio Inc. shRNA発現のための有効な新規ループ配列
WO2008035481A1 (fr) 2006-09-19 2008-03-27 Mitsubishi Electric Corporation Actionneur
US20090318453A1 (en) 2006-09-22 2009-12-24 Taiho Pharmaceutical Co., Ltd Postoperative adjuvant chemotherapy for gastric cancer
WO2008124927A1 (en) * 2007-04-13 2008-10-23 Vincent Research & Consulting Inc. Sirna against thymidylate synthase and uses thereof in cancer treatment regimens
JP2009086463A (ja) 2007-10-01 2009-04-23 Fuji Xerox Co Ltd 無端状部材回転駆動装置および画像形成装置

Also Published As

Publication number Publication date
EP2415870A9 (en) 2012-07-11
US8524876B2 (en) 2013-09-03
JPWO2010113844A1 (ja) 2012-10-11
JP5687188B2 (ja) 2015-03-18
HUE029171T2 (hu) 2017-02-28
EP2415870B1 (en) 2016-07-20
US20120016012A1 (en) 2012-01-19
RU2011143753A (ru) 2013-05-10
EP2415870A1 (en) 2012-02-08
KR20110135980A (ko) 2011-12-20
RU2535993C2 (ru) 2014-12-20
WO2010113844A1 (ja) 2010-10-07
CN107090454A (zh) 2017-08-25
TW201038278A (en) 2010-11-01
HRP20161293T1 (hr) 2016-11-18
KR101579638B1 (ko) 2015-12-22
PT2415870T (pt) 2016-10-25
TWI526211B (zh) 2016-03-21
DK2415870T3 (en) 2016-10-10
CN102369283A (zh) 2012-03-07
PL2415870T3 (pl) 2017-01-31
EP2415870A4 (en) 2012-11-28

Similar Documents

Publication Publication Date Title
ES2599153T3 (es) Molécula de ARNi para la timidilato sintasa y uso de la misma
ES2548018T3 (es) Composiciones que comprenden 2,3,3,3-tetrafluoropropeno e hidrocarburos y usos de las mismas
AR090869A1 (es) COMPOSICIONES DE ARNi DE SERPINC1 Y SUS METODOS DE USO
ES2481040T3 (es) Oligonucleótidos inmunoestimulantes
AR121312A2 (es) AGENTES DE ARNi, COMPOSICIONES Y MÉTODOS DE USO DE LOS MISMOS PARA TRATAR ENFERMEDADES ASOCIADAS CON TRANSTIRETINA (TTR)
PE20210921A1 (es) Agentes arni como inhibidores de la transcripcion de la serpina 1 y composiciones que los comprenden
CY1120495T1 (el) Ολιγονουκλεοτιδικα anaλoγα που στοχευουν το ανθρωπινο lmna
CO2017007335A2 (es) Supresión del gen de la huntingtina inducida por la arni
WO2010033247A3 (en) Reduced size self-delivering rnai compounds
CY1116944T1 (el) Συνθεσεις και μεθοδοι για την αναστολη εκφρασης τρανσθυρετινης
WO2011008730A3 (en) Chemical modification of small hairpin rnas for inhibition of gene expression
MX355408B (es) Inhibidores a base de oligonucleotidos que comprenden un motivo de acido nucleico bloqueado.
BRPI0907008A2 (pt) Métodos otimizados para liberação de dsrna alvejando o gene pcsk9
MX2011013421A (es) Arnds formulado con lipido de direccionamiento del gen pcsk9.
ECSP099794A (es) INHIBICIÓN DE ARNi DE EXPRESIÓN DE ALFA-ENaC
ECSP11010803A (es) Compuestos de cobre nanoparticulados
AR069821A1 (es) Composiciones y metodos de uso de la interferencia de arn para el control de nematodos
ES2549192T3 (es) Construcción de expresión de ADN
IT1399742B1 (it) Microorganismi inattivati contenenti molecole di rna a doppio filamento (dsrna), loro uso come pesticidi e metodi per la loro preparazione
CR11601A (es) Pirimidil ciclopentanos hidroxilados en forma de inhibidores de akt proteina quinasa
CL2011000292A1 (es) Compuestos derivados de enfumafungina y sus sales farmaceuticamente aceptables, como inhibidores de (1,3)-beta-d-glucano sintasa; composicion farmaceutica; y uso para tratar una infeccion fungica.
ES2536640T3 (es) Alteración dirigida de ADN con oligonucleótidos
FR2926818B1 (fr) siRNA CATIONIQUES, SYNTHESE ET UTILISATION POUR L'ARN INTERFERENCE
PA8814801A1 (es) Triazolopiridazinas como inhibidores de par1, su preparación y su uso como medicamentos
AR127137A2 (es) Composiciones de arni de serpinc1 y sus métodos de uso