ES2575008T8 - Nuevas cepas de levadura mutantes capaces de acumular una gran cantidad de lípidos - Google Patents

Nuevas cepas de levadura mutantes capaces de acumular una gran cantidad de lípidos Download PDF

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Publication number
ES2575008T8
ES2575008T8 ES09784257.9T ES09784257T ES2575008T8 ES 2575008 T8 ES2575008 T8 ES 2575008T8 ES 09784257 T ES09784257 T ES 09784257T ES 2575008 T8 ES2575008 T8 ES 2575008T8
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ES
Spain
Prior art keywords
lipids
accumulating
large amount
yeast strains
mutant yeast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09784257.9T
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English (en)
Other versions
ES2575008T3 (es
Inventor
Jean-Marc Nicaud
Thierry Chardot
Athanasios Beopoulos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut National de la Recherche Agronomique INRA
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut National de la Recherche Agronomique INRA filed Critical Centre National de la Recherche Scientifique CNRS
Application granted granted Critical
Publication of ES2575008T3 publication Critical patent/ES2575008T3/es
Publication of ES2575008T8 publication Critical patent/ES2575008T8/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ES09784257.9T 2008-07-11 2009-07-08 Nuevas cepas de levadura mutantes capaces de acumular una gran cantidad de lípidos Active ES2575008T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0854786 2008-07-11
FR0854786 2008-07-11
PCT/FR2009/000848 WO2010004141A2 (fr) 2008-07-11 2009-07-08 Nouvelles souches de levure mutantes capables d'accumuler une grande quantité de lipides

Publications (2)

Publication Number Publication Date
ES2575008T3 ES2575008T3 (es) 2016-06-23
ES2575008T8 true ES2575008T8 (es) 2016-07-05

Family

ID=40348006

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09784257.9T Active ES2575008T3 (es) 2008-07-11 2009-07-08 Nuevas cepas de levadura mutantes capaces de acumular una gran cantidad de lípidos

Country Status (6)

Country Link
US (1) US8597931B2 (es)
EP (1) EP2310517B1 (es)
CA (1) CA2729626C (es)
DK (1) DK2310517T3 (es)
ES (1) ES2575008T3 (es)
WO (1) WO2010004141A2 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2927089B1 (fr) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
DK2310517T3 (en) 2008-07-11 2016-06-06 Inst Nat De La Rech Agronomique (Inra) Novel mutant yeast strains having a capability to accumulate a large amount of lipids
FR2962133B1 (fr) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
FR3002774A1 (fr) 2013-03-04 2014-09-05 Agronomique Inst Nat Rech Levures mutantes ayant une production accrue de lipides et d'acide citrique
FR3005317B1 (fr) 2013-05-02 2016-03-18 Agronomique Inst Nat Rech Levures mutantes capables de produire un acide gras inhabituel
DE102013209950A1 (de) 2013-05-28 2014-12-04 Technische Universität Dresden Hefestamm und Verfahren zur Produktion von Lycopin
EP3155094A1 (en) 2014-06-11 2017-04-19 Institut National De La Recherche Agronomique (INRA) Improved lipid accumulation in yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof
EP3085788A1 (en) 2015-04-23 2016-10-26 Institut National De La Recherche Agronomique Mutant yeast strain capable of degrading cellobiose
EP3106520A1 (en) 2015-06-17 2016-12-21 Institut National De La Recherche Agronomique Mutant yarrowia strain capable of degrading galactose
EP3348647A1 (en) 2017-01-13 2018-07-18 Institut National De La Recherche Agronomique Mutant yeast strain capable of producing medium chain fatty acids
EP3360956A1 (en) 2017-02-10 2018-08-15 Institut National De La Recherche Agronomique Mutant yeast strain capable of degrading cellulose
WO2018200837A1 (en) * 2017-04-27 2018-11-01 Heliae Development Llc Single-stage fermentation methods of culturing microorganisms
CN108441431B (zh) * 2018-03-27 2019-08-30 南京工业大学 一株高抗逆性解脂耶氏酵母及其应用
CN115786149B (zh) * 2022-08-02 2025-06-06 江南大学 一种生产母乳脂质替代品酿酒酵母菌株及其应用

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US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4937189A (en) 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
US5254466A (en) 1989-11-06 1993-10-19 Henkel Research Corporation Site-specific modification of the candida tropicals genome
ATE238425T1 (de) 1993-07-23 2003-05-15 Dsm Nv Selektionmarker-genfreie rekombinante stämme: verfahren zur ihrer herstellung und die verwendung dieser stämme
US6051431A (en) 1994-07-22 2000-04-18 Dsm N.V. Selection marker gene free recombinant strains: a method for obtaining them and the use of these strains
EP0747484A1 (en) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Upstream activator sequences and recombinant promoter sequences functional in yarrowia and vectors containing them
WO1997044470A1 (en) 1996-05-21 1997-11-27 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
DK0814165T3 (da) 1996-06-21 2003-07-21 Suntory Ltd Fremgangsmåde til konstruktion af transformerede gærceller, der ikke indeholder et selekterbart markørgen
FR2782733B1 (fr) 1998-09-01 2002-02-08 Agronomique Inst Nat Rech Procede de transformation non-homologue de yarrowia lipolytica
FR2784394B1 (fr) 1998-10-09 2001-01-05 Lesaffre & Cie Cassette d'integration-excision
PT1276874E (pt) 2000-04-28 2008-01-11 Mayoly Spindler Lab Clonagem e expressão de uma lipase extracelular resistente aos ácidos de yarrowia lipolytica
US6673613B2 (en) 2000-07-26 2004-01-06 Cognis Corporation Use of CYP52A2A promoter to increase gene expression in yeast
EP1506302A4 (en) 2002-05-23 2006-02-08 Cognis Ip Man Gmbh CANDIDA TROPICALIS BLOCKED BY OXIDATION BETA NON REVERSIBLE
JP2004179160A (ja) 2002-11-26 2004-06-24 Samsung Sdi Co Ltd リチウム−硫黄電池用正極
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7459546B2 (en) 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
AU2004290051A1 (en) 2003-11-12 2005-05-26 E.I. Dupont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
US7202356B2 (en) 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
DK1766023T3 (da) 2004-06-04 2010-12-13 Fluxome Sciences As Metabolisk manipulerede celler til fremstillingen af polyumættede fedtsyrer
AU2005267202B2 (en) 2004-06-25 2011-10-27 Corteva Agriscience Llc Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7264949B2 (en) 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
TWI237867B (en) 2004-10-29 2005-08-11 Taiwan Semiconductor Mfg Method of improving to deposit dielectric
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
FR2879215B1 (fr) 2004-12-15 2010-08-20 Inst Francais Du Petrole Production d'acides dicarboxyliques par des souches mutantes ameliorees de yarrowia lipolytica
EP2090558B1 (en) * 2006-10-30 2017-01-11 Kyocera Corporation Slide member
FR2927089B1 (fr) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
DK2310517T3 (en) 2008-07-11 2016-06-06 Inst Nat De La Rech Agronomique (Inra) Novel mutant yeast strains having a capability to accumulate a large amount of lipids
EP2327776A1 (en) 2009-11-30 2011-06-01 Institut National De La Recherche Agronomique Method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation with a recombinant Yarrowia sp
FR2962133B1 (fr) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides

Also Published As

Publication number Publication date
WO2010004141A2 (fr) 2010-01-14
EP2310517B1 (fr) 2016-03-16
EP2310517A2 (fr) 2011-04-20
US20110183387A1 (en) 2011-07-28
CA2729626C (fr) 2017-12-12
ES2575008T3 (es) 2016-06-23
WO2010004141A3 (fr) 2010-04-22
US8597931B2 (en) 2013-12-03
DK2310517T3 (en) 2016-06-06
CA2729626A1 (fr) 2010-01-14

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