ES2571835T3 - Moléculas y métodos con plantilla para el uso de tales moléculas - Google Patents

Moléculas y métodos con plantilla para el uso de tales moléculas

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Publication number
ES2571835T3
ES2571835T3 ES07114663T ES07114663T ES2571835T3 ES 2571835 T3 ES2571835 T3 ES 2571835T3 ES 07114663 T ES07114663 T ES 07114663T ES 07114663 T ES07114663 T ES 07114663T ES 2571835 T3 ES2571835 T3 ES 2571835T3
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molecules
template
methods
templated
template molecules
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Henrik Pedersen
Alex Haahr Gouilaev
Christian Klarner Sams
Frank Abilgaard Sløk
Per-Ola Freskgård
Anette Holtmann
Olsen Eva Kampmann
Gitte Nystrup Husemoen
Jakob Felding
Thomas Franch
Thomas Thisted
Lene Hyldtoft
Mads Nørregaard-Madsen
Michael Anders Godskesen
Glad Sanne Schrøder
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Nuevolution AS
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Nuevolution AS
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    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D405/00Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/04Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
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    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
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    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
    • C07H19/10Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
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    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • C07H19/20Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1062Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1068Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
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    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C40B50/04Methods of creating libraries, e.g. combinatorial synthesis using dynamic combinatorial chemistry techniques
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    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00452Means for the recovery of reactants or products
    • B01J2219/00454Means for the recovery of reactants or products by chemical cleavage from the solid support
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00596Solid-phase processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068Means for controlling the apparatus of the process
    • B01J2219/00702Processes involving means for analysing and characterising the products
    • B01J2219/00707Processes involving means for analysing and characterising the products separated from the reactor apparatus
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    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
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    • B01J2219/0072Organic compounds
    • B01J2219/00722Nucleotides
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    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718Type of compounds synthesised
    • B01J2219/0072Organic compounds
    • B01J2219/00729Peptide nucleic acids [PNA]
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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
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  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Un método para sintetizar una composición de moléculas de diferentes plantillas que comprenden una pluralidad de grupos funcionales, comprendiendo dicho método las etapas de i) proporcionar una pluralidad de plantillas que tienen diferentes elementos de codificación y / o un orden diferente de elementos de codificación, dichas plantillas que comprenden una pluralidad de nucleótidos y una secuencia de 3 a 100 elementos de codificación, en el que cada elemento de codificación comprende al menos un grupo de reconocimiento capaz de reconocer un predeterminado elemento que complementa, y en el que cada elemento de codificación comprende o consiste en de 4 a 100 subunidades, ii) proporcionar una pluralidad de bloques de construcción, en el que cada bloque comprende la construcción a) al menos un elemento de complementación que comprende al menos un grupo de reconocimiento capaz de reconocer un elemento de codificación predeterminado, b) al menos una entidad funcional que comprende al menos un grupo funcional y al menos un grupo reactivo, y c) al menos un enlazador que separa la al menos una entidad funcional desde el al menos un elemento que complementa, iii) poner en contacto cada uno de dichos elementos de codificación con un elemento de complementación capaz de reconocer dicha codificación elemento, dicho elemento comprende una pluralidad de nucleótidos complementando, iv) formar una plantilla complementando que comprende al menos 3 elementos de complementación en la forma de un lineal secuencia de elementos que complementan por enzimáticamente que une dicho por lo menos 3 elementos de complementación, y v) la obtención de una composición de diferentes moléculas con plantilla comprendiendo cada uno unido covalentemente, grupos funcionales mediante la vinculación de un grupo funcional de al menos una entidad funcional a un grupo funcional de otra entidad funcional, en donde la molécula de plantilla está unida por medio de un conector a la plantilla o la plantilla complementaria a la templated la síntesis de la molécula de plantilla, y en el que la síntesis de las moléculas con plantilla no implica traducción mediada por ribosomas de un ácido nucleico.
ES07114663T 2001-06-20 2002-06-20 Moléculas y métodos con plantilla para el uso de tales moléculas Expired - Lifetime ES2571835T3 (es)

Applications Claiming Priority (2)

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DKPA200100962 2001-06-20
DKPA200200415 2002-03-15

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EP (3) EP1402024B1 (es)
JP (1) JP2004535193A (es)
CN (1) CN1539014A (es)
AT (1) ATE371026T1 (es)
CA (1) CA2451524A1 (es)
CY (2) CY1117518T1 (es)
DE (1) DE60221986D1 (es)
DK (3) DK1402024T3 (es)
ES (2) ES2571835T3 (es)
IL (1) IL159268A0 (es)
NO (1) NO20035226L (es)
NZ (1) NZ530098A (es)
RU (1) RU2004101289A (es)
WO (1) WO2002103008A2 (es)

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EP1905829B1 (en) 2016-02-17
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RU2004101289A (ru) 2005-07-10
NO20035226D0 (no) 2003-11-24
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EP1905829A2 (en) 2008-04-02
DK1402024T3 (da) 2007-12-27
IL159268A0 (en) 2004-06-01
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EP1402024A2 (en) 2004-03-31
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CY1117518T1 (el) 2017-04-26
EP1905829A3 (en) 2009-06-24
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EP2305808B1 (en) 2016-03-02
ATE371026T1 (de) 2007-09-15

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