ES2548133T3 - Método de detección de niveles muy bajos de analito en una muestra fluida de película delgada contenida en una cámara de pequeño espesor - Google Patents

Método de detección de niveles muy bajos de analito en una muestra fluida de película delgada contenida en una cámara de pequeño espesor Download PDF

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ES2548133T3
ES2548133T3 ES09730905.8T ES09730905T ES2548133T3 ES 2548133 T3 ES2548133 T3 ES 2548133T3 ES 09730905 T ES09730905 T ES 09730905T ES 2548133 T3 ES2548133 T3 ES 2548133T3
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target analyte
chamber
fluid sample
sample
biological fluid
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Stephen C. Wardlaw
Robert A. Levine
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Abbott Point of Care Inc
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Abbott Point of Care Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Materials Engineering (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Un método para realizar un inmunoensayo de una muestra de fluido biológico para la cuantificación de un analito diana en una cámara de muestra de película delgada en el que la cámara tiene un espesor de 10 μm o menos, comprendiendo dicho método las etapas de: proporcionar una pluralidad de anticuerpos o ligandos (6) de captura específicos del analito diana, suficiente para unir todo el analito (12) diana añadido, los cuales están fijos a una superficie (4) de una cámara de prueba (2) de la muestra de película delgada o a estructuras inmovilizadas en la cámara de análisis (2), siendo dichos anticuerpos o ligandos (6) de captura específicos de un primer epítopo o epítopos en las moléculas (12) de analito diana que están presentes en dicha muestra de fluido biológico; llenar dicha cámara (2) de prueba de la muestra con una mezcla de dicha muestra de fluido biológico y nanopartículas fluorescentes (8, 8', 10, 10') acopladas a anticuerpos que se unen selectivamente a un segundo epítopo o epítopos de las moléculas (12) de analito diana, que están presentes en dicha muestra de fluido biológico; y fotografiar dicha muestra quiescente en dicha cámara (2) de prueba de la muestra y contar las moléculas de analito diana (12) que son capturadas por dichos anticuerpos o ligandos (6) de captura inmóviles y haciéndolos detectables fotografiando las nanopartículas fluorescentes inmovilizadas (10, 10') acopladas a anticuerpos que están unidos a un segundo epítopo sobre el analito diana inmovilizado (12).

Description

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Claims (1)

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ES09730905.8T 2008-04-09 2009-04-02 Método de detección de niveles muy bajos de analito en una muestra fluida de película delgada contenida en una cámara de pequeño espesor Active ES2548133T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43571 1987-04-27
US4357108P 2008-04-09 2008-04-09
PCT/US2009/039283 WO2009126505A1 (en) 2008-04-09 2009-04-02 Method of detecting very low levels of analyte within a thin film fluid sample contained in a thin thickness chamber

Publications (1)

Publication Number Publication Date
ES2548133T3 true ES2548133T3 (es) 2015-10-14

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ES09730905.8T Active ES2548133T3 (es) 2008-04-09 2009-04-02 Método de detección de niveles muy bajos de analito en una muestra fluida de película delgada contenida en una cámara de pequeño espesor

Country Status (7)

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US (1) US20090258371A1 (es)
EP (1) EP2281197B1 (es)
JP (1) JP5539314B2 (es)
CN (1) CN102047116A (es)
CA (1) CA2720072C (es)
ES (1) ES2548133T3 (es)
WO (1) WO2009126505A1 (es)

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Publication number Publication date
EP2281197B1 (en) 2015-09-02
EP2281197A1 (en) 2011-02-09
JP2011518324A (ja) 2011-06-23
US20090258371A1 (en) 2009-10-15
WO2009126505A1 (en) 2009-10-15
JP5539314B2 (ja) 2014-07-02
CA2720072C (en) 2013-11-19
CN102047116A (zh) 2011-05-04
CA2720072A1 (en) 2009-10-15

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