ES2547715T3 - Análisis de ácido nucleico - Google Patents

Análisis de ácido nucleico Download PDF

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Publication number
ES2547715T3
ES2547715T3 ES10800649.5T ES10800649T ES2547715T3 ES 2547715 T3 ES2547715 T3 ES 2547715T3 ES 10800649 T ES10800649 T ES 10800649T ES 2547715 T3 ES2547715 T3 ES 2547715T3
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Spain
Prior art keywords
rna
microvesicles
urine
nucleic acid
extraction
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ES10800649.5T
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English (en)
Spanish (es)
Inventor
Leileata M. Russo
Kevin C. Miranda
Johan Skog
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General Hospital Corp
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General Hospital Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D249/00—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms
    • C07D249/02—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms not condensed with other rings
    • C07D249/08—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/12—Antidiuretics, e.g. drugs for diabetes insipidus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
ES10800649.5T 2009-07-16 2010-07-16 Análisis de ácido nucleico Active ES2547715T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22602509P 2009-07-16 2009-07-16
US22610609P 2009-07-16 2009-07-16
US226106P 2009-07-16
US226025P 2009-07-16
PCT/US2010/042365 WO2011009104A1 (en) 2009-07-16 2010-07-16 Nucleic acid analysis

Publications (1)

Publication Number Publication Date
ES2547715T3 true ES2547715T3 (es) 2015-10-08

Family

ID=43449850

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10800649.5T Active ES2547715T3 (es) 2009-07-16 2010-07-16 Análisis de ácido nucleico

Country Status (13)

Country Link
US (3) US20110195426A1 (cg-RX-API-DMAC7.html)
EP (1) EP2454588B1 (cg-RX-API-DMAC7.html)
JP (3) JP5909444B2 (cg-RX-API-DMAC7.html)
KR (2) KR20180029105A (cg-RX-API-DMAC7.html)
CN (2) CN102498397B (cg-RX-API-DMAC7.html)
AU (1) AU2010273928A1 (cg-RX-API-DMAC7.html)
BR (1) BR112012001077A2 (cg-RX-API-DMAC7.html)
CA (1) CA2768371A1 (cg-RX-API-DMAC7.html)
DK (1) DK2454588T3 (cg-RX-API-DMAC7.html)
ES (1) ES2547715T3 (cg-RX-API-DMAC7.html)
IN (1) IN2012DN00866A (cg-RX-API-DMAC7.html)
SG (2) SG2014014617A (cg-RX-API-DMAC7.html)
WO (1) WO2011009104A1 (cg-RX-API-DMAC7.html)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007103572A2 (en) * 2006-03-09 2007-09-13 Aethlon Medical, Inc. Extracorporeal removal of microvesicular particles
US11266736B2 (en) 2008-04-17 2022-03-08 Vin De Bona Trading Company Pte Ltd Method of painting micro vesicles
US20130029339A1 (en) 2009-09-09 2013-01-31 The General Hospital Corporation Use of microvesicles in analyzing kras mutations
WO2011031877A1 (en) 2009-09-09 2011-03-17 The General Hospital Corporation Use of microvesicles in analyzing nucleic acid profiles
JP5755326B2 (ja) * 2010-06-11 2015-07-29 日立化成株式会社 腎機能の特徴を決定する方法
WO2012031008A2 (en) 2010-08-31 2012-03-08 The General Hospital Corporation Cancer-related biological materials in microvesicles
WO2012064993A1 (en) * 2010-11-10 2012-05-18 Exosome Diagnosties, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
US20120196285A1 (en) * 2011-01-31 2012-08-02 Esoterix Genetic Laboratories, Llc Methods for Enriching Microparticles or Nucleic Acids Using Binding Molecules
EP3569607A1 (en) 2011-05-11 2019-11-20 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
EP2717989B1 (en) 2011-06-10 2018-05-30 Hitachi Chemical Co., Ltd. Vesicle capturing devices and methods for using same
EP2748335B1 (en) 2011-08-22 2018-10-03 Exosome Diagnostics, Inc. Urine biomarkers
US10174361B2 (en) * 2011-11-10 2019-01-08 Exosome Diagnostics, Inc. Cerebrospinal fluid assay
CN104995311A (zh) 2012-08-30 2015-10-21 外来体诊断公司 用于核酸测定的对照
CA2887058C (en) * 2012-10-03 2022-02-22 Exosome Diagnostics, Inc. Use of microvesicles in diagnosis, prognosis, and treatment of medical diseases and conditions
CN104736724A (zh) * 2012-10-05 2015-06-24 日立化成株式会社 尿液外泌小体mRNA和使用其检测糖尿病型肾病的方法
US20150233800A1 (en) 2012-10-11 2015-08-20 Vin-De-Bona Trading Company Pte Ltd Method of painting microvesicles
ES2949637T3 (es) 2013-03-13 2023-10-02 Univ Miami Método de aislamiento y purificación de microvesículas a partir de sobrenadantes de cultivos celulares y fluidos biológicos
US9662649B2 (en) 2013-05-06 2017-05-30 Hitachi Chemical Company America, Ltd. Devices and methods for capturing target molecules
BR112016002716B1 (pt) 2013-08-06 2023-02-28 Exosome Diagnostics, Inc Método para diagnóstico ou monitoramento de um progresso ou recorrência de um câncer de próstata com escore de gleason alto
WO2015069790A1 (en) * 2013-11-06 2015-05-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method for subtyping lymphoma types by means expression profiling
WO2015082372A1 (en) * 2013-12-02 2015-06-11 Albert-Ludwigs-Universität Freiburg Process for the diagnosis of cancer by using exosomes
WO2015153732A2 (en) 2014-04-01 2015-10-08 Cornell University Use of double-stranded dna in exosomes: a novel biomarker in cancer detection
CA2946384A1 (en) * 2014-04-22 2015-10-29 Trovagene, Inc. Concentrating nucleic acids in urine
CN104031915A (zh) * 2014-06-17 2014-09-10 复旦大学附属肿瘤医院 用于结直肠癌预后的基因片段及其应用
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
JP6631852B2 (ja) 2014-11-12 2020-01-15 日立化成株式会社 臓器障害を診断するための方法および装置
WO2016172598A1 (en) * 2015-04-22 2016-10-27 The Broad Institute Inc. Exosomes and uses thereof
GB2539497A (en) 2015-06-19 2016-12-21 Airbus Operations Ltd A Rotational Joint For An Aircraft Folding Wing
DE112016003948T5 (de) 2015-08-31 2018-05-09 City Of Sapporo Molekulare verfahren zum beurteilen einer urothelialen erkrankung
WO2017181161A1 (en) 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
WO2018057820A1 (en) 2016-09-21 2018-03-29 Predicine, Inc. Systems and methods for combined detection of genetic alterations
CN109642229A (zh) * 2016-05-13 2019-04-16 外来体诊断公司 从生物液体分离细胞外囊泡和共分离无细胞dna的自动和手动方法
WO2018213392A1 (en) 2017-05-17 2018-11-22 Exosome Diagnostics, Inc. Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection
WO2019014486A1 (en) 2017-07-12 2019-01-17 Exosome Diagnostics, Inc. METHODS FOR ISOLATING AND ENRICHING POPULATIONS OF EXTRACELLULAR VESICLES DERIVED FROM BIOFLUIDS, AND METHODS OF USE THEREOF
JP7069587B2 (ja) * 2017-07-31 2022-05-18 東ソー株式会社 分析機器の洗浄方法
WO2019116234A1 (en) * 2017-12-12 2019-06-20 Nestec S.A. Methods for determining microbiome ribosomal rna composition changes
JP7444386B2 (ja) * 2018-06-07 2024-03-06 株式会社Ｌｓｉメディエンス ヒト尿からのマイクロベシクルの分離方法及び分析方法
EP3884045B1 (en) 2018-11-20 2023-01-11 Exosome Diagnostics, Inc. Methods for internal controls of microvesicle isolations
CN109856259A (zh) * 2018-12-29 2019-06-07 南通大学附属医院 一种血清胰腺癌外泌体特异蛋白及其再验证过程
CN114958834A (zh) * 2020-03-09 2022-08-30 中山大学 一种adar蛋白细胞内高效结合底物及应用
EP4158065A1 (en) 2020-05-29 2023-04-05 Exosome Diagnostics, Inc. Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
IL300361A (en) 2020-08-15 2023-04-01 Regeneron Pharma will fall into obesity in patients with variants of nucleic acid compounds encoding the calcitonin receptor
WO2023288130A1 (en) 2021-07-16 2023-01-19 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
EP4479561A1 (en) 2022-02-18 2024-12-25 Exosome Diagnostics, Inc. Use of microvesicle signatures in the identification and treatment of renal disorders
CN114480656B (zh) * 2022-03-07 2023-12-26 温州医科大学附属眼视光医院 Slc12a3和/或slc12a9在作为葡萄膜黑色素瘤治疗和预后检测指标中的应用
EP4584397A1 (en) 2022-09-07 2025-07-16 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5840867A (en) 1991-02-21 1998-11-24 Gilead Sciences, Inc. Aptamer analogs specific for biomolecules
CA2218875C (en) 1991-07-23 2000-11-07 The Research Foundation Of State University Of New York Improvements in the in situ pcr
US5582981A (en) 1991-08-14 1996-12-10 Gilead Sciences, Inc. Method for identifying an oligonucleotide aptamer specific for a target
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5556773A (en) 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
PT938320E (pt) * 1996-03-26 2010-09-22 Michael S Kopreski Método que permite a utilização de arn extracelular extraído de plasma ou de soro para detectar, monitorizar ou avaliar o cancro
AU735416B2 (en) 1996-11-06 2001-07-05 Sequenom, Inc. Dna diagnostics based on mass spectrometry
US6994960B1 (en) 1997-05-28 2006-02-07 The Walter And Eliza Hall Institute Of Medical Research Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage
US6004755A (en) 1998-04-07 1999-12-21 Incyte Pharmaceuticals, Inc. Quantitative microarray hybridizaton assays
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
FR2788780B1 (fr) 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
ATE428688T1 (de) * 2000-01-28 2009-05-15 Mip Technologies Ab Funktionelle monomere zur molekularen erkennung und katalyse
US20040241176A1 (en) * 2000-04-27 2004-12-02 Ap Cells. Inc. Method of producing membrane vesicles
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
US6913879B1 (en) 2000-07-10 2005-07-05 Telechem International Inc. Microarray method of genotyping multiple samples at multiple LOCI
US6525154B1 (en) 2000-07-20 2003-02-25 The Regents Of The University Of California Molecular imprinting for the recognition of peptides in aqueous solution
CA2414782C (en) * 2001-05-11 2012-10-09 Regents Of The University Of Minnesota Intron associated with myotonic dystrophy type 2 and methods of use
WO2002099064A2 (en) * 2001-06-05 2002-12-12 Gene Logic, Inc. Rna purification methods
US6696271B2 (en) 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins
WO2003023065A1 (en) 2001-09-06 2003-03-20 Syngenta Participations Ag Dna methylation patterns
US20030162190A1 (en) 2001-11-15 2003-08-28 Gorenstein David G. Phosphoromonothioate and phosphorodithioate oligonucleotide aptamer chip for functional proteomics
ES2431944T3 (es) 2002-06-26 2013-11-28 Cold Spring Harbor Laboratory Métodos para determinar los perfiles de metilación
WO2004020667A1 (en) 2002-09-02 2004-03-11 Pamgene B.V. Novel integrated microarray analysis
US7141371B2 (en) 2002-09-06 2006-11-28 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods for detecting and localizing DNA mutations by microarray
EP1498144A1 (en) * 2003-07-15 2005-01-19 Universite Pierre Et Marie Curie Paris Vi Extracellular vesicles from non-pathogenic amoebae useful as vehicle for transferring a molecule of interest to an eukaryotic cell
US7384589B2 (en) 2003-08-01 2008-06-10 Lawrence Livermore National Security, Llc Nanoscale molecularly imprinted polymers and method thereof
US8021847B2 (en) * 2004-06-02 2011-09-20 Proxy Life Science Holdings, Inc. Microvesicle-based compositions and methods
WO2005121369A2 (en) * 2004-06-02 2005-12-22 Sourcepharm, Inc. Rna-containing microvesicles and methods therefor
KR20070058441A (ko) * 2004-07-01 2007-06-08 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 면역억제성 엑소솜
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20080187923A1 (en) 2005-04-15 2008-08-07 Cedars-Sinai Medical Center Flow-Cytometric Heteroduplex Analysis for Detection of Genetic Alterations
WO2006119439A2 (en) * 2005-05-03 2006-11-09 Althea Technologies, Inc. Compositions and methods for the analysis of degraded nucleic acids
CA2643322C (en) * 2006-02-24 2015-07-21 Novartis Ag Microparticles containing biodegradable polymer and cationic polysaccharide for use in immunogenic compositions
JP2008035779A (ja) * 2006-08-07 2008-02-21 Mitsubishi Rayon Co Ltd 核酸の分解度を測定する方法と核酸アレイ
US20080287669A1 (en) * 2007-05-16 2008-11-20 Braman Jeffrey C Methods and compositions for identifying compounds useful in nucleic acid purification
EP2613149B1 (en) * 2007-07-25 2014-09-17 University Of Louisville Research Foundation, Inc. Exosome-associated microRNA as a diagnostic marker
JP5769417B2 (ja) * 2007-08-16 2015-08-26 ザ ロイヤル インスティチューション フォー ザ アドバンスメント オブ ラーニング／マギル ユニバーシティ 腫瘍細胞由来微小胞
EP2198296B1 (en) * 2007-09-05 2015-11-11 Laurentian University Method of using tumour rna integrity to measure response to chemotherapy in cancer patients
ES2419129T3 (es) * 2007-09-14 2013-08-19 The Ohio State University Research Foundation Expresión de miARN en microvesículas de sangre periférica humana y usos del mismo
KR20100127768A (ko) * 2008-02-01 2010-12-06 더 제너럴 하스피탈 코포레이션 의학적 질환 및 병태의 진단, 예후, 및 치료에 있어서 미세소포체의 용도

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Publication number Publication date
DK2454588T3 (en) 2015-09-28
JP2017131244A (ja) 2017-08-03
IN2012DN00866A (cg-RX-API-DMAC7.html) 2015-07-10
EP2454588B1 (en) 2015-07-15
KR20120037992A (ko) 2012-04-20
SG2014014617A (en) 2014-05-29
AU2010273928A2 (en) 2012-02-09
CN102498397A (zh) 2012-06-13
EP2454588A4 (en) 2012-12-12
US20110195426A1 (en) 2011-08-11
JP5909444B2 (ja) 2016-04-26
HK1171083A1 (zh) 2013-03-15
SG177677A1 (en) 2012-02-28
WO2011009104A1 (en) 2011-01-20
AU2010273928A1 (en) 2012-02-09
EP2454588A1 (en) 2012-05-23
JP2016116512A (ja) 2016-06-30
CN102498397B (zh) 2016-04-06
CN105779436A (zh) 2016-07-20
KR20180029105A (ko) 2018-03-19
US20160348095A1 (en) 2016-12-01
JP2012533308A (ja) 2012-12-27
US20170114389A1 (en) 2017-04-27
CA2768371A1 (en) 2011-01-20
BR112012001077A2 (pt) 2020-08-11
HK1173213A1 (en) 2013-05-10

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