ES2526925T3 - Métodos para la detección mejorada de moléculas pequeñas de RNA - Google Patents

Métodos para la detección mejorada de moléculas pequeñas de RNA Download PDF

Info

Publication number
ES2526925T3
ES2526925T3 ES08838229.6T ES08838229T ES2526925T3 ES 2526925 T3 ES2526925 T3 ES 2526925T3 ES 08838229 T ES08838229 T ES 08838229T ES 2526925 T3 ES2526925 T3 ES 2526925T3
Authority
ES
Spain
Prior art keywords
amplification
modified
base
duplex
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08838229.6T
Other languages
English (en)
Spanish (es)
Inventor
Igor Vassily Kutyavin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2526925T3 publication Critical patent/ES2526925T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/117Modifications characterised by incorporating modified base
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/207Modifications characterised by siRNA, miRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES08838229.6T 2007-10-09 2008-10-08 Métodos para la detección mejorada de moléculas pequeñas de RNA Active ES2526925T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97865707P 2007-10-09 2007-10-09
US978657P 2007-10-09
PCT/US2008/079169 WO2009048928A1 (en) 2007-10-09 2008-10-08 Methods, compositions and kits for the improved detection of small rna molecules

Publications (1)

Publication Number Publication Date
ES2526925T3 true ES2526925T3 (es) 2015-01-16

Family

ID=40242697

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08838229.6T Active ES2526925T3 (es) 2007-10-09 2008-10-08 Métodos para la detección mejorada de moléculas pequeñas de RNA

Country Status (7)

Country Link
US (2) US8637276B2 (enExample)
EP (1) EP2198059B1 (enExample)
JP (1) JP5486501B2 (enExample)
AU (1) AU2008310955B2 (enExample)
CA (1) CA2702584A1 (enExample)
ES (1) ES2526925T3 (enExample)
WO (1) WO2009048928A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8637276B2 (en) * 2007-10-09 2014-01-28 Igor Vassily Kutyavin Methods, compositions and kits for the improved detection of small RNA molecules
US9074248B1 (en) * 2012-12-18 2015-07-07 Qiagen Gmbh Primers for helicase dependent amplification and their methods of use
KR20160098097A (ko) * 2015-02-09 2016-08-18 (주) 하임바이오텍 마이크로알앤에이 검출용 키트 및 방법
WO2017087943A1 (en) * 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
US10913986B2 (en) 2016-02-01 2021-02-09 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
WO2018016885A1 (ko) * 2016-07-21 2018-01-25 주식회사 하임바이오텍 알앤에이 검출용 키트 및 방법
JP7055691B2 (ja) * 2017-07-11 2022-04-18 株式会社東芝 短鎖核酸伸長用プライマーセット、アッセイキット、短鎖核酸伸長方法、増幅方法及び検出方法
ES3030914T3 (en) * 2019-11-04 2025-07-02 Mirnax Biosens S L Bivalent reverse primer
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
CN118166080B (zh) * 2024-04-15 2025-04-04 苏州市立医院 一种无RNA逆转录酶的miRNA一步法检测方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6218118B1 (en) * 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
JP2003525292A (ja) 2000-03-01 2003-08-26 エポック・バイオサイエンシーズ・インコーポレイテッド ミスマッチ識別のための修飾オリゴヌクレオチド
CA2562390C (en) 2004-04-07 2014-12-02 Exiqon A/S Novel methods for quantification of micrornas and small interfering rnas
WO2007127992A2 (en) 2006-04-28 2007-11-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates
US8637276B2 (en) * 2007-10-09 2014-01-28 Igor Vassily Kutyavin Methods, compositions and kits for the improved detection of small RNA molecules

Also Published As

Publication number Publication date
US8980558B2 (en) 2015-03-17
CA2702584A1 (en) 2009-04-16
US8637276B2 (en) 2014-01-28
AU2008310955B2 (en) 2015-01-29
WO2009048928A1 (en) 2009-04-16
US20140170666A1 (en) 2014-06-19
EP2198059B1 (en) 2014-12-03
US20100304442A1 (en) 2010-12-02
AU2008310955A1 (en) 2009-04-16
JP2011501663A (ja) 2011-01-13
EP2198059A1 (en) 2010-06-23
JP5486501B2 (ja) 2014-05-07

Similar Documents

Publication Publication Date Title
ES2526925T3 (es) Métodos para la detección mejorada de moléculas pequeñas de RNA
ES2700606T3 (es) Uso de trifosfatos de desoxinucleósidos modificados en sus bases para mejorar la detección de ácidos nucleicos
US9487824B2 (en) Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences
US8143006B2 (en) Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
US9121056B2 (en) Use of products of PCR amplification carrying elements of secondary structure to improve PCR-based nucleic acid detection
CN105339505B (zh) 基于通用报告体的基因分型方法和材料
US9914963B2 (en) Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
US20110171649A1 (en) Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
ES2575378T3 (es) Métodos y reactivos para reducir la amplificación no específica
ES2886605T3 (es) Procedimiento genérico para la estabilización de ARN específico
ES2966013T3 (es) Diseño específico de alelo de cebadores cooperativos para genotipado de variantes de ácido nucleico mejorado
US20210388431A1 (en) Thermokinetically balanced isothermal amplification of nucleic acid sequences