ES2524351T3 - Método para la identificación electroquímica de secuencias diana de nucleótidos - Google Patents

Método para la identificación electroquímica de secuencias diana de nucleótidos Download PDF

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Publication number
ES2524351T3
ES2524351T3 ES09757698.7T ES09757698T ES2524351T3 ES 2524351 T3 ES2524351 T3 ES 2524351T3 ES 09757698 T ES09757698 T ES 09757698T ES 2524351 T3 ES2524351 T3 ES 2524351T3
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Prior art keywords
oxidizable
target sequences
nucleotide target
reducible compound
electric current
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ES09757698.7T
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English (en)
Inventor
Benoît LIMOGES
Thibaut Defever
Damien Marchal
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Centre National de la Recherche Scientifique CNRS
Universite Paris Diderot Paris 7
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Centre National de la Recherche Scientifique CNRS
Universite Paris Diderot Paris 7
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Método de identificación electroquímica de secuencias diana de nucleótidos, siendo el método del tipo según el cual: se proporciona una muestra biológica susceptible de contener una secuencia diana de nucleótidos determinada; se proporcionan medios de amplificación activables que comprenden nucleótidos libres, para provocar la replicación de dicha secuencia diana de nucleótidos determinada y la formación de secuencias diana de nucleótidos replicadas bajo la forma de dobles cadenas de ácidos nucleicos; se proporciona un compuesto oxido-reducible apto para reaccionar frente a dichos nucleótidos y se pone en contacto dicho compuesto oxido-reducible con dicha muestra biológica; se activan dichos medios de amplificación activables; se aplica un campo eléctrico a la muestra para activar dicho compuesto oxido-reducible y se mide la corriente eléctrica que atraviesa la muestra representativa de la actividad electroquímica de dicho compuesto oxido-reducible; y se determina la presencia de dicha secuencia diana de nucleótidos determinada si la corriente eléctrica disminuye; caracterizado porque se proporciona un compuesto oxido-reducible que se intercala durante la replicación entre los nucleótidos que forman dichas secuencias diana replicadas, provocando dichas secuencias diana replicadas la inhibición de la actividad electroquímica de dicho compuesto oxido-reducible intercalado debido a lo cual la corriente eléctrica disminuye.

Description

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Claims (1)

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ES09757698.7T 2008-06-05 2009-06-04 Método para la identificación electroquímica de secuencias diana de nucleótidos Active ES2524351T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0803143A FR2932191B1 (fr) 2008-06-05 2008-06-05 Methode d'identification electrochimique de sequences cibles de nucleotides.
FR0803143 2008-06-05
PCT/FR2009/000653 WO2009147322A1 (fr) 2008-06-05 2009-06-04 Méthode d'identification électrochimique de séquences cibles de nucléotides

Publications (1)

Publication Number Publication Date
ES2524351T3 true ES2524351T3 (es) 2014-12-05

Family

ID=40193801

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09757698.7T Active ES2524351T3 (es) 2008-06-05 2009-06-04 Método para la identificación electroquímica de secuencias diana de nucleótidos

Country Status (11)

Country Link
US (2) US8962240B2 (es)
EP (1) EP2288731B1 (es)
JP (1) JP5536762B2 (es)
KR (1) KR101710728B1 (es)
CN (1) CN102112630B (es)
BR (1) BRPI0913446A2 (es)
CA (1) CA2726028C (es)
ES (1) ES2524351T3 (es)
FR (1) FR2932191B1 (es)
RU (1) RU2532855C2 (es)
WO (1) WO2009147322A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011056606B3 (de) * 2011-12-19 2013-01-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
CN102937613B (zh) * 2012-11-03 2015-07-01 福建医科大学 一种基于亚甲基蓝指示剂定量检测pcr的电化学安培检测法
JP2015171338A (ja) * 2014-03-12 2015-10-01 学校法人慶應義塾 核酸のリアルタイム検出方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6200752B1 (en) * 1998-01-14 2001-03-13 Joseph R. Lakowicz Method and composition for detecting the presence of a nucleic acid sequence in a sample
US6063573A (en) * 1998-01-27 2000-05-16 Clinical Micro Sensors, Inc. Cycling probe technology using electron transfer detection
DE19964220C2 (de) * 1998-11-23 2003-07-03 Friz Biochem Gmbh Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche
US6833267B1 (en) * 1998-12-30 2004-12-21 Clinical Micro Sensors, Inc. Tissue collection devices containing biosensors
US6824974B2 (en) * 2001-06-11 2004-11-30 Genorx, Inc. Electronic detection of biological molecules using thin layers
US20040197801A1 (en) * 2002-11-15 2004-10-07 Liu Timothy Z. Nucleic acid sequence detection
US7479557B2 (en) * 2004-06-10 2009-01-20 Agency For Science, Technology +Research DNA threading intercalators
JP5320077B2 (ja) * 2006-03-03 2013-10-23 ユニベルシテ パリ ディドロ−パリ ７ 標的核酸配列の電気化学的検出方法
US20080125333A1 (en) * 2006-05-31 2008-05-29 Antara Biosciences Inc. Devices and kits for detecting one or more target agents

Also Published As

Publication number Publication date
JP5536762B2 (ja) 2014-07-02
WO2009147322A1 (fr) 2009-12-10
RU2532855C2 (ru) 2014-11-10
RU2010149453A (ru) 2012-06-10
KR20110016912A (ko) 2011-02-18
FR2932191A1 (fr) 2009-12-11
US20150125870A1 (en) 2015-05-07
CA2726028A1 (fr) 2009-12-10
US9856526B2 (en) 2018-01-02
CA2726028C (fr) 2017-01-10
CN102112630A (zh) 2011-06-29
US20110136126A1 (en) 2011-06-09
EP2288731B1 (fr) 2014-08-20
US8962240B2 (en) 2015-02-24
KR101710728B1 (ko) 2017-02-27
BRPI0913446A2 (pt) 2020-08-04
EP2288731A1 (fr) 2011-03-02
FR2932191B1 (fr) 2010-12-03
CN102112630B (zh) 2018-02-27
JP2011523556A (ja) 2011-08-18

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