ES2490613T3 - ARMET como marcador de cáncer - Google Patents
ARMET como marcador de cáncer Download PDFInfo
- Publication number
- ES2490613T3 ES2490613T3 ES09798890.1T ES09798890T ES2490613T3 ES 2490613 T3 ES2490613 T3 ES 2490613T3 ES 09798890 T ES09798890 T ES 09798890T ES 2490613 T3 ES2490613 T3 ES 2490613T3
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- ES
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- Prior art keywords
- cancer
- armet
- phase
- sample
- cancer marker
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57423—Specifically defined cancers of lung
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/475—Assays involving growth factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Hospice & Palliative Care (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Oncology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Un método para evaluar el cáncer de pulmón, cáncer de colon, cáncer de mama o cáncer de ovario in vitro, que comprende medir en una muestra la concentración de: (a) proteína ARMET (rica en arginina, mutada en tumores en fase tempranas) y/o fragmentos de la misma. (b) opcionalmente uno o más marcadores adicionales de cáncer, y (c) usar el resultado de la medición de la fase (a) y opcionalmente de la fase (b) en la evaluación del cáncer, donde dicha muestra es suero o plasma, y donde un incremento de la concentración de una proteína ARMET y/o fragmentos de la misma es indicativo de cáncer de pulmón, cáncer de colon, cáncer de mama o cáncer de ovario
Description
Análisis por LC-ESI-MSMS:
La digestión tríptica (100 µl) se separa mediante una HPLC bidimensional (MudPIT) en un sistema Nano-LC (Ultimate, Famos, Switchos; LC Packings, Idstein, Alemania). La separación se realiza con columnas bidimensionales 5 auto-empaquetadas (Sílice fundida: PicoFrit 75 µm, New Objective; RP: ProntoSil 120-5-C18 AQ+, Bischoff; SCX: Partisil 10, Whatman). Se generan 11 fracciones SCX mediante la elución en fases con cantidades crecientes sucesivamente de NH4Ac (de 0 a 1.500 mM). Estas se separan posteriormente en la parte RP de la columna y se analizan en línea usando escaneos dependientes de datos con una trampa de iones ESI-MS (LCQ deca XP; Thermo Electron, Massachusetts, E.E.U.U.; véanse los parámetros en la tabla X). Para cada muestra se realizan tres series.
10 Los datos brutos se procesan con un sistema no comercial de manejo de datos propio de Roche que usa Sequest como algoritmo de base (véase los parámetros en la Tab. X). Se combinaron las listas resultantes de péptidos identificados y proteínas de las series de réplicas.
La proteína ARMET se identifica con ayuda de las secuencias identificadas y que se dan en la Tab. 2. 15 Detección de ARMET como un marcador potencial de cáncer de pulmón:
Para cada paciente, se comparan las proteínas identificadas y el número de péptidos correspondientes de la muestra del tumor con los resultados que corresponden del tejido adyacente normal. De esta manera, se descubre que la 20 proteína ARMET está presente específicamente o es muy abundante en tejido tumoral y no es detectable o es difícilmente detectable en el tejido sano control.
Tabla 1: Adquisición de datos por MSMS y parámetros de búsqueda de bases de datos
- Adquisición de datos por MSMS
- Exclusión MS 350-2000 Da para iones precursores
- Recuento de repeticiones
- 2
- Duración de la repetición
- 0,25 min
- Tamaño de lista de exclusión
- 50
- Duración de la exclusión
- 5 min
- Amplitud de la masa de exclusión
- bajo 0,5 Da, alto 1,5 Da
- Sequest
- Número de iones 30
- Intensidad mínima de los iones
- 10.000 unidades
- Tolerancia de masa del precursor
- 1,5 Da
- Tolerancia de masa del fragmento
- 1,5 Da
- Xcorr
- >1,8; 2,3, 2,8 (z = 1; 2; 3)
- dCn
- > 0,1
- Sp
- > 500
- Bases de datos
- Humangp (ensamblada por Roche Bioinformatics)
25 La proteína ARMET está fuertemente sobre-representada en tejido tumoral de pacientes que padecen cáncer de pulmón. Las siguientes secuencias peptídicas de la proteína ARMET se identifican mediante la búsqueda en el formulario de la base de datos “LCQ-MS2-data” en tejido tumoral:
Usando el método descrito anteriormente se han identificado las siguientes secuencias derivadas de ARMET. 30 Tabla 2: Secuencias identificadas por ESI-MSMS
- Secuencia identificada
- Tramo de aminoácidos de ARMET (cf. SEC ID Nº: 1)
- • DRDVTFSPATIENELIK
- • 43-59
- • DVTFSPATIENELIK
- • 45-59
- • IINEVSKPLAHHIPVEK
- • 85-101
- • LCYYIGATDDAATK
- • 71-84
17
Claims (1)
-
imagen1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP08022238 | 2008-12-22 | ||
EP08022238 | 2008-12-22 | ||
PCT/EP2009/009159 WO2010072383A1 (en) | 2008-12-22 | 2009-12-18 | Armet as a marker for cancer |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2490613T3 true ES2490613T3 (es) | 2014-09-04 |
Family
ID=40474747
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09798890.1T Active ES2490613T3 (es) | 2008-12-22 | 2009-12-18 | ARMET como marcador de cáncer |
Country Status (7)
Country | Link |
---|---|
US (3) | US8741587B2 (es) |
EP (1) | EP2380024B1 (es) |
JP (1) | JP5368579B2 (es) |
CN (1) | CN102317784B (es) |
CA (1) | CA2747942C (es) |
ES (1) | ES2490613T3 (es) |
WO (1) | WO2010072383A1 (es) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102375057A (zh) * | 2010-08-10 | 2012-03-14 | 复旦大学 | 一种均相荧光同时检测多种血清标志物的方法 |
EP2684050B1 (en) * | 2011-03-11 | 2016-05-11 | Roche Diagnostics GmbH | Armet as marker for chronic obstructive pulmonary disease (copd) |
CA2861541C (en) | 2012-01-24 | 2021-11-30 | University Of Massachusetts | Soluble manf in pancreatic beta-cell disorders |
NZ703411A (en) | 2012-06-27 | 2017-09-29 | Berg Llc | Use of markers in the diagnosis and treatment of prostate cancer |
EP3011334A1 (en) * | 2013-06-20 | 2016-04-27 | The Trustees Of The University Of Pennsylvania | Methods for diagnosing pancreatic cancer |
EP3022561B1 (en) * | 2013-07-15 | 2019-08-28 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Method for the prognosis of survival time of a patient suffering from a solid cancer |
CN104677998A (zh) * | 2013-11-29 | 2015-06-03 | 沈阳药科大学 | 血浆评估肺癌的生物标记物 |
EP3123172B1 (en) * | 2014-03-24 | 2021-01-13 | Instrumentation Laboratory Company | Bioassay system and method for detecting analytes in body fluids |
CN106659765B (zh) | 2014-04-04 | 2021-08-13 | 德玛医药 | 二脱水半乳糖醇及其类似物或衍生物用于治疗非小细胞肺癌和卵巢癌的用途 |
US20170157243A1 (en) * | 2014-06-24 | 2017-06-08 | University Of Massachusetts | MANF as a Regulator of Immune System Function |
AU2015360694B2 (en) | 2014-12-08 | 2021-10-14 | Berg Llc | Use of markers including filamin a in the diagnosis and treatment of prostate cancer |
CN105891482B (zh) * | 2016-03-29 | 2017-12-19 | 复旦大学附属中山医院 | 一种基于生物标志物谱针对中国农村人口肺结节人群的肺癌风险预测模型 |
CN105717147B (zh) * | 2016-03-29 | 2018-11-23 | 复旦大学附属中山医院 | 一种基于ct影像及生物标志物谱针对中国城市人口肺结节人群的肺癌风险预测试剂盒 |
WO2017201225A1 (en) * | 2016-05-19 | 2017-11-23 | Poc Medical Systems, Inc. | Cancer screening via detection and quantification of multiple biomarkers |
CN106645725B (zh) * | 2017-01-06 | 2018-07-24 | 安徽医科大学 | 基于manf作为标记物的肝细胞肝癌和肝内胆管细胞癌鉴别产品及方法 |
CN117173083A (zh) * | 2022-07-22 | 2023-12-05 | 浙江省肿瘤医院 | 基于舌象图像和肿瘤标志物的肿瘤预测系统、方法及应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7189507B2 (en) * | 2001-06-18 | 2007-03-13 | Pdl Biopharma, Inc. | Methods of diagnosis of ovarian cancer, compositions and methods of screening for modulators of ovarian cancer |
WO2004057336A2 (en) | 2002-12-20 | 2004-07-08 | Roche Diagnostics Gmbh | Use of nicotinamide n-methyltransferase as a marker for colorectal cancer |
EP1631682A1 (en) * | 2003-06-04 | 2006-03-08 | Agency for Science, Technology and Research | Differentially regulated hepatocellular carcinoma genes and uses thereof |
-
2009
- 2009-12-18 WO PCT/EP2009/009159 patent/WO2010072383A1/en active Application Filing
- 2009-12-18 CA CA2747942A patent/CA2747942C/en not_active Expired - Fee Related
- 2009-12-18 ES ES09798890.1T patent/ES2490613T3/es active Active
- 2009-12-18 CN CN200980152833.7A patent/CN102317784B/zh not_active Expired - Fee Related
- 2009-12-18 JP JP2011541227A patent/JP5368579B2/ja not_active Expired - Fee Related
- 2009-12-18 EP EP09798890.1A patent/EP2380024B1/en not_active Not-in-force
-
2011
- 2011-05-09 US US13/103,123 patent/US8741587B2/en active Active
-
2014
- 2014-04-14 US US14/251,788 patent/US20140219998A1/en not_active Abandoned
-
2016
- 2016-09-08 US US15/259,392 patent/US20160377625A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CA2747942A1 (en) | 2010-07-01 |
JP2012513016A (ja) | 2012-06-07 |
WO2010072383A8 (en) | 2010-12-29 |
US20160377625A1 (en) | 2016-12-29 |
EP2380024B1 (en) | 2014-06-11 |
US20110212465A1 (en) | 2011-09-01 |
JP5368579B2 (ja) | 2013-12-18 |
CN102317784B (zh) | 2014-07-16 |
EP2380024A1 (en) | 2011-10-26 |
CA2747942C (en) | 2016-12-06 |
WO2010072383A1 (en) | 2010-07-01 |
CN102317784A (zh) | 2012-01-11 |
US8741587B2 (en) | 2014-06-03 |
US20140219998A1 (en) | 2014-08-07 |
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