ES2490613T3 - ARMET como marcador de cáncer - Google Patents

ARMET como marcador de cáncer Download PDF

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Publication number
ES2490613T3
ES2490613T3 ES09798890.1T ES09798890T ES2490613T3 ES 2490613 T3 ES2490613 T3 ES 2490613T3 ES 09798890 T ES09798890 T ES 09798890T ES 2490613 T3 ES2490613 T3 ES 2490613T3
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Prior art keywords
cancer
armet
phase
sample
cancer marker
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ES09798890.1T
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English (en)
Inventor
Markus Roessler
Johann Karl
Michael Tacke
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57423—Specifically defined cancers of lung
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Hospice & Palliative Care (AREA)
  • Food Science & Technology (AREA)
  • Oncology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un método para evaluar el cáncer de pulmón, cáncer de colon, cáncer de mama o cáncer de ovario in vitro, que comprende medir en una muestra la concentración de: (a) proteína ARMET (rica en arginina, mutada en tumores en fase tempranas) y/o fragmentos de la misma. (b) opcionalmente uno o más marcadores adicionales de cáncer, y (c) usar el resultado de la medición de la fase (a) y opcionalmente de la fase (b) en la evaluación del cáncer, donde dicha muestra es suero o plasma, y donde un incremento de la concentración de una proteína ARMET y/o fragmentos de la misma es indicativo de cáncer de pulmón, cáncer de colon, cáncer de mama o cáncer de ovario

Description

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Análisis por LC-ESI-MSMS:
La digestión tríptica (100 µl) se separa mediante una HPLC bidimensional (MudPIT) en un sistema Nano-LC (Ultimate, Famos, Switchos; LC Packings, Idstein, Alemania). La separación se realiza con columnas bidimensionales 5 auto-empaquetadas (Sílice fundida: PicoFrit 75 µm, New Objective; RP: ProntoSil 120-5-C18 AQ+, Bischoff; SCX: Partisil 10, Whatman). Se generan 11 fracciones SCX mediante la elución en fases con cantidades crecientes sucesivamente de NH4Ac (de 0 a 1.500 mM). Estas se separan posteriormente en la parte RP de la columna y se analizan en línea usando escaneos dependientes de datos con una trampa de iones ESI-MS (LCQ deca XP; Thermo Electron, Massachusetts, E.E.U.U.; véanse los parámetros en la tabla X). Para cada muestra se realizan tres series.
10 Los datos brutos se procesan con un sistema no comercial de manejo de datos propio de Roche que usa Sequest como algoritmo de base (véase los parámetros en la Tab. X). Se combinaron las listas resultantes de péptidos identificados y proteínas de las series de réplicas.
La proteína ARMET se identifica con ayuda de las secuencias identificadas y que se dan en la Tab. 2. 15 Detección de ARMET como un marcador potencial de cáncer de pulmón:
Para cada paciente, se comparan las proteínas identificadas y el número de péptidos correspondientes de la muestra del tumor con los resultados que corresponden del tejido adyacente normal. De esta manera, se descubre que la 20 proteína ARMET está presente específicamente o es muy abundante en tejido tumoral y no es detectable o es difícilmente detectable en el tejido sano control.
Tabla 1: Adquisición de datos por MSMS y parámetros de búsqueda de bases de datos
Adquisición de datos por MSMS
Exclusión MS 350-2000 Da para iones precursores
Recuento de repeticiones
2
Duración de la repetición
0,25 min
Tamaño de lista de exclusión
50
Duración de la exclusión
5 min
Amplitud de la masa de exclusión
bajo 0,5 Da, alto 1,5 Da
Sequest
Número de iones 30
Intensidad mínima de los iones
10.000 unidades
Tolerancia de masa del precursor
1,5 Da
Tolerancia de masa del fragmento
1,5 Da
Xcorr
>1,8; 2,3, 2,8 (z = 1; 2; 3)
dCn
> 0,1
Sp
> 500
Bases de datos
Humangp (ensamblada por Roche Bioinformatics)
25 La proteína ARMET está fuertemente sobre-representada en tejido tumoral de pacientes que padecen cáncer de pulmón. Las siguientes secuencias peptídicas de la proteína ARMET se identifican mediante la búsqueda en el formulario de la base de datos “LCQ-MS2-data” en tejido tumoral:
Usando el método descrito anteriormente se han identificado las siguientes secuencias derivadas de ARMET. 30 Tabla 2: Secuencias identificadas por ESI-MSMS
Secuencia identificada
Tramo de aminoácidos de ARMET (cf. SEC ID Nº: 1)
• DRDVTFSPATIENELIK
• 43-59
• DVTFSPATIENELIK
• 45-59
• IINEVSKPLAHHIPVEK
• 85-101
• LCYYIGATDDAATK
• 71-84
17
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Claims (1)

  1. imagen1
ES09798890.1T 2008-12-22 2009-12-18 ARMET como marcador de cáncer Active ES2490613T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08022238 2008-12-22
EP08022238 2008-12-22
PCT/EP2009/009159 WO2010072383A1 (en) 2008-12-22 2009-12-18 Armet as a marker for cancer

Publications (1)

Publication Number Publication Date
ES2490613T3 true ES2490613T3 (es) 2014-09-04

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES09798890.1T Active ES2490613T3 (es) 2008-12-22 2009-12-18 ARMET como marcador de cáncer

Country Status (7)

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US (3) US8741587B2 (es)
EP (1) EP2380024B1 (es)
JP (1) JP5368579B2 (es)
CN (1) CN102317784B (es)
CA (1) CA2747942C (es)
ES (1) ES2490613T3 (es)
WO (1) WO2010072383A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102375057A (zh) * 2010-08-10 2012-03-14 复旦大学 一种均相荧光同时检测多种血清标志物的方法
CN103403556B (zh) * 2011-03-11 2015-06-24 霍夫曼-拉罗奇有限公司 Armet作为慢性阻塞性肺病(copd)的标志物
US9891231B2 (en) * 2012-01-24 2018-02-13 University Of Massachusetts Soluble MANF in pancreatic β-cell disorders
KR20150023904A (ko) 2012-06-27 2015-03-05 버그 엘엘씨 전립선암의 진단 및 치료에서의 마커의 용도
WO2014205374A1 (en) * 2013-06-20 2014-12-24 The Trustees Of The University Of Pennsylvania Methods for diagnosing pancreatic cancer
US9851357B2 (en) * 2013-07-15 2017-12-26 Inserm (Institute National De La Sante Et De La Recherche Medicale Method for the prognosis of survival time of a patient suffering from a solid cancer
CN104677998A (zh) * 2013-11-29 2015-06-03 沈阳药科大学 血浆评估肺癌的生物标记物
US20150268237A1 (en) * 2014-03-24 2015-09-24 Josef Kerimo Bioassay system and method for detecting analytes in body fluids
EP3125920B1 (en) 2014-04-04 2020-12-23 Del Mar Pharmaceuticals Dianhydrogalactitol, diacetyldianhydrogalactitol or dibromodulcitol to treat non-small-cell carcinoma of the lung and ovarian cancer
US20170157243A1 (en) * 2014-06-24 2017-06-08 University Of Massachusetts MANF as a Regulator of Immune System Function
JP6759229B2 (ja) 2014-12-08 2020-09-23 バーグ エルエルシー 前立腺癌の診断および処置におけるフィラミンａを含むマーカーの使用
CN105891482B (zh) * 2016-03-29 2017-12-19 复旦大学附属中山医院 一种基于生物标志物谱针对中国农村人口肺结节人群的肺癌风险预测模型
CN105717147B (zh) * 2016-03-29 2018-11-23 复旦大学附属中山医院 一种基于ct影像及生物标志物谱针对中国城市人口肺结节人群的肺癌风险预测试剂盒
WO2017201225A1 (en) * 2016-05-19 2017-11-23 Poc Medical Systems, Inc. Cancer screening via detection and quantification of multiple biomarkers
CN106645725B (zh) * 2017-01-06 2018-07-24 安徽医科大学 基于manf作为标记物的肝细胞肝癌和肝内胆管细胞癌鉴别产品及方法
CN117173083A (zh) * 2022-07-22 2023-12-05 浙江省肿瘤医院 基于舌象图像和肿瘤标志物的肿瘤预测系统、方法及应用

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US7189507B2 (en) * 2001-06-18 2007-03-13 Pdl Biopharma, Inc. Methods of diagnosis of ovarian cancer, compositions and methods of screening for modulators of ovarian cancer
MXPA05006382A (es) 2002-12-20 2005-10-24 Hoffmann La Roche Uso de nicotinamida n-metiltransferasa como un marcador para cancer colorrectal.
WO2004108964A1 (en) * 2003-06-04 2004-12-16 Agency For Science, Technology And Research Differentially regulated hepatocellular carcinoma genes and uses thereof

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EP2380024B1 (en) 2014-06-11
JP2012513016A (ja) 2012-06-07
US8741587B2 (en) 2014-06-03
CA2747942A1 (en) 2010-07-01
JP5368579B2 (ja) 2013-12-18
CN102317784A (zh) 2012-01-11
US20140219998A1 (en) 2014-08-07
CA2747942C (en) 2016-12-06
WO2010072383A8 (en) 2010-12-29
US20110212465A1 (en) 2011-09-01
US20160377625A1 (en) 2016-12-29
EP2380024A1 (en) 2011-10-26
WO2010072383A1 (en) 2010-07-01
CN102317784B (zh) 2014-07-16

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