ES2477141R1 - Sistema basado en un vector viral de expresión transitoria que permite múltiples aplicaciones - Google Patents
Sistema basado en un vector viral de expresión transitoria que permite múltiples aplicaciones Download PDFInfo
- Publication number
- ES2477141R1 ES2477141R1 ES201290074A ES201290074A ES2477141R1 ES 2477141 R1 ES2477141 R1 ES 2477141R1 ES 201290074 A ES201290074 A ES 201290074A ES 201290074 A ES201290074 A ES 201290074A ES 2477141 R1 ES2477141 R1 ES 2477141R1
- Authority
- ES
- Spain
- Prior art keywords
- multiple applications
- viral
- system based
- vectors
- allows multiple
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
Abstract
Sistema basado en un vector viral de expresión transitoria que permite múltiples aplicaciones.#Se describen unos vectores virales adecuados para su transfección en árboles leñosos con el fin de transportar y expresar genes beneficiosos. Se ejemplifican específicamente vectores para transfectar árboles de cítricos. Los vectores permiten la expresión de proteínas útiles, tales como aquellas que pueden proteger a los árboles de enfermedades. Se ejemplifican específicamente métodos para transfectar plantas leñosas que permiten múltiples aplicaciones de los vectores mientras se evita la exclusión de la superinfección.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US32197010P | 2010-04-08 | 2010-04-08 | |
US61/321,970 | 2010-04-08 | ||
US201161440445P | 2011-02-08 | 2011-02-08 | |
US61/440,445 | 2011-02-08 | ||
PCT/US2011/031671 WO2011127340A2 (en) | 2010-04-08 | 2011-04-08 | Viral-based transient-expression vector system that allows multiple applications |
Publications (3)
Publication Number | Publication Date |
---|---|
ES2477141A2 ES2477141A2 (es) | 2014-07-15 |
ES2477141R1 true ES2477141R1 (es) | 2014-08-06 |
ES2477141B2 ES2477141B2 (es) | 2015-02-23 |
Family
ID=44763557
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201290074A Active ES2477141B2 (es) | 2010-04-08 | 2011-04-08 | Sistema basado en un vector viral de expresión transitoria que permite múltiples aplicaciones |
Country Status (5)
Country | Link |
---|---|
CN (2) | CN105087638B (es) |
BR (1) | BRPI1101853A2 (es) |
ES (1) | ES2477141B2 (es) |
WO (1) | WO2011127340A2 (es) |
ZA (1) | ZA201208259B (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015031130A1 (en) * | 2013-08-28 | 2015-03-05 | Southern Gardens Citrus | Citrus plants resistant to citrus huanglongbing (ex greening) caused by candidatus liberibacter asiaticus (las) and bacterial canker caused by (xanthomonas axonopodis pv. citri) (xac) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040029280A1 (en) * | 1997-03-14 | 2004-02-12 | Selective Genetics, Inc. | Viral vectors with modified tropism |
US20040087537A1 (en) * | 2002-09-19 | 2004-05-06 | Molmed Spa | Compositions comprising fusion polypeptides HIV-Nef and NGF, and methods of using the same |
WO2009099877A2 (en) * | 2008-01-31 | 2009-08-13 | State Of Oregon Acting By And Through The State Board Of Higher Educ. On Behalf Of Oregon State University | Closterovirus vectors and methods |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1500394A1 (en) * | 2003-07-22 | 2005-01-26 | Polichem S.A. | Pharmaceutical compositions comprising ascorbic acid or the treatment of fungal superinfections and fungal recurrences |
JP5992654B2 (ja) * | 2007-12-21 | 2016-09-14 | ニュートリー株式会社 | 整腸及び/又は便通改善のための組成物 |
CN101270395A (zh) * | 2008-05-08 | 2008-09-24 | 重庆大学 | 柑桔衰退病毒一步法实时荧光反转录聚合酶链式反应固相化试剂盒及其检测方法 |
-
2011
- 2011-04-08 CN CN201510204207.5A patent/CN105087638B/zh active Active
- 2011-04-08 CN CN201180027897.1A patent/CN102933713B/zh not_active Expired - Fee Related
- 2011-04-08 ES ES201290074A patent/ES2477141B2/es active Active
- 2011-04-08 WO PCT/US2011/031671 patent/WO2011127340A2/en active Application Filing
- 2011-04-08 BR BRPI1101853-4A patent/BRPI1101853A2/pt not_active Application Discontinuation
-
2012
- 2012-11-05 ZA ZA2012/08259A patent/ZA201208259B/en unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040029280A1 (en) * | 1997-03-14 | 2004-02-12 | Selective Genetics, Inc. | Viral vectors with modified tropism |
US20040087537A1 (en) * | 2002-09-19 | 2004-05-06 | Molmed Spa | Compositions comprising fusion polypeptides HIV-Nef and NGF, and methods of using the same |
WO2009099877A2 (en) * | 2008-01-31 | 2009-08-13 | State Of Oregon Acting By And Through The State Board Of Higher Educ. On Behalf Of Oregon State University | Closterovirus vectors and methods |
Non-Patent Citations (2)
Title |
---|
EHRENGRUBER MARKUS U et al. Semliki forest virus vectors with mutations in the nonstructural protein 2 gene permit extended superinfection of neuronal and non-neuronal cells.Journal of Neurovirology AUG 2007 00/08/2007 VOL: 13 No: 4 Pags: 353-363 ISSN 1355-0284 Doi: doi:10.1080/13550280701393204 * |
FOLIMONOVA SVETLANA Y et al. Infection with Strains of Citrus Tristeza Virus Does Not Exclude Superinfection by Other Strains of the Virus.Journal of Virology FEB 2010 00/02/2010 VOL: 84 No: 3 Pags: 1314-1325 ISSN 0022-538X(print) ISSN 1098-5514(electronic) Doi: doi:10.1128/JVI.02075-09 * |
Also Published As
Publication number | Publication date |
---|---|
ES2477141A2 (es) | 2014-07-15 |
ZA201208259B (en) | 2013-07-31 |
CN105087638A (zh) | 2015-11-25 |
ES2477141B2 (es) | 2015-02-23 |
CN102933713A (zh) | 2013-02-13 |
CN105087638B (zh) | 2019-07-12 |
CN102933713B (zh) | 2015-05-13 |
BRPI1101853A2 (pt) | 2015-08-11 |
WO2011127340A3 (en) | 2012-08-09 |
WO2011127340A2 (en) | 2011-10-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CL2017002998A1 (es) | Plantas que tienen expresión modificada de thca sintasa | |
CL2014003381A1 (es) | Celulas de planta que expresan las proteinas insecticidas cry1fa y cry2aa asi como los productos y procedimientos que se desprenden de las mismas. | |
DOP2013000240A (es) | Composiciones y métodos para transferencia de energía mitocondrial de línea germinal autóloga | |
AR103056A1 (es) | Métodos para conferir o aumentar la resistencia a los herbicidas en plantas y/o algas con variantes de protoporfirinógeno oxidasa | |
UY34663A (es) | Acido nucleico y proteina recombinante del gen de la endotoxina axmi345, vectores, células, composiciones y métodos para proteger plantas | |
CL2017002962A1 (es) | Superficie de césped híbrido y soporte para la misma. | |
UY34662A (es) | Acido nucleico y proteina recombinante del gen de la toxina axmi335, vectores, células, composiciones y métodos para proteger plantas | |
SV2016005264A (es) | Proteínas de fusión tatk-cdkl5, composiciones, formulaciones y uso de estas | |
AR120102A2 (es) | Genes de tolerancia a herbicidas y métodos para usar los mismos | |
CR20150375A (es) | Elementos reguladores de plantas y usos de los mismos | |
BR112015030105A2 (pt) | Construção de gene quimérico, vetor recombinante, usos dos mesmos, bem como método para a produção de uma planta monocotiledônea com maior rendimento em comparação com uma planta monocotiledônea do tipo selvagem correspondente | |
BR112015009340A2 (pt) | Transformação de plastídeo usando vetores de dna lineares | |
UY34551A (es) | Sistema automático y métodos para separar y singularizar embriones de planta | |
BR112014006794A2 (pt) | vetores com base em vírus da tristeza dos citros para expressão de gene/s estranho/s | |
UY35783A (es) | Elementos regulatorios de zea mays y usos de los mismos. | |
ES2477141R1 (es) | Sistema basado en un vector viral de expresión transitoria que permite múltiples aplicaciones | |
BR112017000376A2 (pt) | composições e métodos para aumentar o desenvolvimento vegetal e seu rendimento | |
CO2017007985A2 (es) | Genes de acetohidroxiácido sintasa mutados en euphorbiaceae y material vegetal que comprende dichos genes | |
AR101239A1 (es) | Almacenamiento y manipulación de embriones de plantas | |
UY35782A (es) | Elementos regulatorios de zea mays y usos de los mismos. | |
MX367917B (es) | Metodos y sistemas para extraer embriones monocotiledoneos. | |
CL2017002400A1 (es) | Un constructo de acidos nucleicos para conferir tolerancia a herbicidas a las plantas | |
Kinney | The discourse of columns | |
AR087578A1 (es) | Metodos para aumentar el contenido de proteinas, aceites y/o aminoacidos en una planta | |
CL2016001674A1 (es) | Casete de expresión génica, que comprende un promotor unido operativamente a un transgen, vectores y células huésped que comprenden dicho casete de expresión, así como método para la expresión de genes heterólogos en plantas. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 2477141 Country of ref document: ES Kind code of ref document: B2 Effective date: 20150223 |